dipeptidyl peptidase iv inhibitory activity of peptides in porcine skin gelatin hydrolysates
Báo cáo y học: "Dipeptidyl peptidase IV activity and/or structure homologs: Contributing factors in the pathogenesis of rheumatoid arthritis" doc
Ngày tải lên :
09/08/2014, 07:20
... enzymatically inactive (DPP6, DPP10) In some instances, the DPPIV activity is clearly intrinsic whereas in others the nature of the DPPIV activity remains debatable In these latter cases, DPPIV activity ... would include the systemic administration of DPPIV inhibitors to reduce circulating activated T cell activity with direct injection of recombinant DPPIV into the inflamed synovium to inactivate ... DPPIV activity in the context of higher circulating levels in the periphery may contribute towards a gradient of functional chemokine activity that will maintain attraction of activated migrating...
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Báo cáo khoa học: Extraenzymatic functions of the dipeptidyl peptidase IV-related proteins DP8 and DP9 in cell adhesion, migration and apoptosis doc
Ngày tải lên :
23/03/2014, 11:20
... function of increased DP9 expression may be to retain expressing cells in the tumor and in sites of expression in the injured liver It would be interesting to localize the DP9-expressing cells in tumors ... Dipeptidyl peptidase IV overexpression induces up-regulation of E-cadherin and tissue inhibitors of matrix metalloproteinases, resulting in decreased invasive potential in ovarian carcinoma cells ... evaluated In addition, the RGD motif of DP9 was replaced with Arg-Ala-Glu (RAE) to investigate whether this integrin-binding motif played a role The RGD integrin-binding motif was first identified in...
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Tài liệu Báo cáo khoa học: Different modes of dipeptidyl peptidase IV (CD26) inhibition by oligopeptides derived from the N-terminus of HIV-1 Tat indicate at least two inhibitor binding sites doc
Ngày tải lên :
20/02/2014, 11:20
... not be explained with GlyPro-R110-CO-(CH2)4Cl In order to examine the binding of inhibitory peptides to the noncompetitive binding site of substrate-loaded DP IV, docking studies of Tat(1–9) ... describing competitive binding of Tat(1–9) directly to the empty active site of DP IV [23], the docking studies demonstrate the possible binding of Tat(1–9) to two different binding sites, one binding ... single amino acid substitutions at certain positions of the parent structure alter the mode of inhibition indicating binding of the peptide to another binding site In addition to differences in...
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Báo cáo khoa học: The dipeptidyl peptidase IV family in cancer and cell biology pot
Ngày tải lên :
06/03/2014, 09:22
... nociception, is inactivated by DPIV enzyme activity [77] DPIV enzyme activity is effective on a number of chemokines in vitro (Table 2) DPIV in cell biology A number of DPIV-binding proteins have been ... costimulatory activity of CD26 involves the ligand-binding domain [124,125] A recent in vivo study using a DPIV-selective inhibitor in DPIV gko mice has further shown independence of immune functions of ... difference in the vicinity of the active site Ala657 in FAP, instead of Asp663 in DPIV, lessens the acidity and increases the size of this pocket, making FAP capable of endopeptidase activity [3] Inhibitors...
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Báo cáo khoa học: Epitope analysis of the rat dipeptidyl peptidase IV monoclonal antibody 6A3 that blocks pericellular fibronectin-mediated cancer cell adhesion pot
Ngày tải lên :
29/03/2014, 22:21
... presence of 6A3 In line with the previous results showing that FN ⁄ DPP IV binding is independent of DPP IV catalytic activity [3], 6A3-binding of rDPP IV also had no effect on its enzymatic activity, ... enzymatic activity of DPP IV [9] However, the binding between DPP IV and pericellular polyFN appears to be independent of this enzymatic activity [3,4] Indeed, the putative FN-binding site in DPP IV ... epitope in both native and denatured rDPP IV, independent of DPP IV enzymatic activity, which is suggestive of a noncompetitive inhibition mechanism The 6A3 epitope in full-length rDPP IV was...
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Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
Ngày tải lên :
08/03/2014, 08:20
... transformation of preg into androstadienol, a precursor in the biosynthesis of 1354 P Soucy et al (Eur J Biochem 270) Fig In uence of increasing amounts of P450red on the relative formation of DHEA ... 17,20-lyase activity In order to determine whether the transformation of preg into androstadienol requires prior synthesis of 17a-OHpreg or DHEA, we performed enzymatic assays using human and porcine ... formation of 17a-OH-preg and DHEA The lack of androstadienol synthesis in the presence of lM of ketoconazole (C), an inhibitor of cytochrome Fig Thin layer chromatography showing the transformation of...
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Báo cáo khoa học: The potent inhibitory activity of histone H1.2 C-terminal fragments on furin doc
Ngày tải lên :
16/03/2014, 13:20
... furin inhibitors and for the mechanistic studies of the regulation of furin activity in vivo Results Purification and identification of the endogenous furin inhibitors from the porcine liver Porcine ... constructing artificial furin inhibitors, we purified three fractions from the porcine liver using a combination of chromatographic techniques They all possessed high inhibitory activity against furin ... inhibitory activity analysis were finished in less than 10 Expression of full-length porcine histone H1.2 and its N- and C-terminal fragments and their inhibitory activity assay toward furin In...
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Báo cáo khoa học: Inhibitory activity of double-sequence analogues of trypsin inhibitor SFTI-1 from sunflower seeds: an example of peptide splicing docx
Ngày tải lên :
29/03/2014, 09:20
... proteinase peptidic inhibitor consisting of 16 amino acids The inhibitor (BiKF) was designed by combining two building loops of BBI and was able to inhibit both trypsin and chymotrypsin independently ... volumes of the trypsin and substrate solutions The chymotrypsin inhibitory activity in the presence of trypsin was determined by the same procedure, using a reverse order of enzyme addition for preincubation ... synthesis and kinetic studies of a bicyclic and bifunctional proteinase peptidic inhibitor consisting of 16 amino acids The inhibitor was designed by combining two binding loops of BBI As postulated...
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Báo cáo y học: "Anti-inflammatory activity of monogalactosyldiacylglycerol in human articular cartilage in vitro: activation of an antiinflammatory cyclooxygenase-2 (COX-2) pathway" doc
Ngày tải lên :
12/08/2014, 17:21
... anti-proliferative activity [4,5], this class of compounds revealed an anti-inflammatory activity in vitro by inhibiting the generation of a superoxide anion in primed leucocytes, showing an inhibitory ... in the mouse chondrocytic cell line MC615 a pathway involving p38 and NF-kB is activated in inflammation [13,15] In order to investigate the possible signaling pathways repressed by MGDG in inflammation ... represses p38 (p38 Mitogen Activated Protein Kinase), involved in IL-6 (Interleukin-6) and IL-8 (Interleukin-8) production A) Inhibition of IL-6 and IL-8 synthesis by the p38 inhibitor SB 203580 Human...
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activate cognition activity of student in physics teaching via using experiment with the aid of computer (demonstrated via chaptercurrent in different environment 11th grade advanced pro
Ngày tải lên :
24/08/2014, 14:01
... REASONING AND PRACTICAL BASES OF ACTIVATION OF COGNITIVE ACTIVITIES IN PHYSICS TEACHING VIA EXPERIMENTING WITH THE AID OF COM 1.1 Activate cognitive activities for students in teaching In the ... thinking, enhancing self-learning, self-studying, selfproblem solving, creative practicing capacity of students…Actively employing advanced, modern method and information technology in teaching ... results in an increase in intensity, speed, frequency of the activity as well as sustainability of cognition process This explain the opinion that positiveness is high degree of cognition It brings...
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Study of GDNF family receptor alpha 2 and inhibitory activity of GDNF family receptor alpha 2b (GFR alpha 2b) isoform
Ngày tải lên :
14/09/2015, 13:13
... will be of great interest in the future if Domain of GFRα1a and GFRα2a can be structurally determined along with the other ligand binding domains (Domain and 3) for a more precise definition of the ... ligand induced neurite outgrowth of GFRα1a, in a Rho-ROCK dependent mechanism Figure 6.6 RhoA dominate negative mutant prevented inhibitory effects of GFRα1b Figure 6.7 Combinatory effect of retinoic ... GFRα2c (lacking exon and 3) In order to gain a better understanding of the biological significance, the expression levels of GFRα2 isoforms in different regions of the human brain were determined and...
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Tài liệu Báo cáo khoa học: Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase pdf
Ngày tải lên :
19/02/2014, 18:20
... there is a type I SPase activity in M pneumoniae, we determined the N-terminus of P40 as it appears in protein extracts of M pneumoniae Determination of the N-terminus of P40 by Edman degradation ... (CAF) [30]: during the first step of the labeling reaction the e-amino groups of lysine are specifically converted to homoarginine In a second step, the free amino terminal groups of the peptides are ... state of this peptide is unexpected: a peptide containing two basic amino acids (arginine and histidine) and a blocked amino terminus should not acquire more than two positive charges in positive...
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Báo cáo khoa học: Ets-1/ Elk-1 is a critical mediator of dipeptidyl-peptidase III transcription in human glioblastoma cells pdf
Ngày tải lên :
29/03/2014, 09:20
... N-terminal domain of Ets-1 is involved in the formation of a complex with other proteins, whereas its C-terminal domain is involved in DNA binding [38] By contrast, the DNA-binding domain of Elk-1 ... binding a heterodimer of Ets-1 and Elk-1 to the probe, and the N-terminus of Ets-1 and the C-terminus of Elk-1 were involved in the formation of the heterodimer Preincubation of nuclear proteins ... III activity is increased with histological aggressiveness of ovarian primary carcinomas Gynecol Oncol 91, 194–200 16 Smyth M & O’Cuinn G (1994) Dipeptidyl aminopeptidase activities of guinea-pig...
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Báo cáo y học: "Pre-clinical development as microbicide of zinc tetra-ascorbo-camphorate, a novel terpenoid derivative: Potent in vitro inhibitory activity against both R5- and X4-tropic HIV-1 strains without significant in vivo mucosal toxicity" docx
Ngày tải lên :
10/08/2014, 05:21
... rating of "minimal," scores of to receive a vaginal irritation rating of "mild," scores of to 11 receive a vaginal irritation rating of "borderline," and scores of 12 to 16, receive a vaginal ... control) Figure into primary C14 Evaluation of cells inhibitory activity on HIV-1 DNA content Evaluation of C14 inhibitory activity on HIV-1 DNA content into primary cells Monocyte-derived macrophages ... to examine the safety and toxicity profiles of C14 derivative Importantly, antiviral concentrations of C14 derivative did not result in detectable levels of inflammation or toxicity in vivo Our...
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ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE AND IN SITU ACTIVITY OF NITRIFYING BIOFILMS
Ngày tải lên :
05/09/2013, 08:40
... distributions of in situ nitrifying activities were determined The relationship between the spatial organization of nitrifying bacterial populations and the in situ activity of these populations within ... Microbial diversity of ammonia-oxidizing bacteria The phylogenetic microbial diversity of two types of nitrifying biofilms, a domestic wastewater and an autotrophic nitrifying biofilms, were determined ... sequential oxidation of NH4+ and NO2- in the oxic biofilm strata, that is, the active NH4+-oxidizing zone is located in the outer part of the oxic biofilm, whereas the active NO2 oxidizing zone is located...
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Tài liệu Báo cáo khoa học: The effect of small molecules in modulating the chaperone activity of aB-crystallin against ordered and disordered protein aggregation pdf
Ngày tải lên :
18/02/2014, 16:20
... overall activity of chaperone proteins Of the target proteins tested, Arg-HCl was found to specifically increase the activity of aB-crystallin against DTT-induced precipitation of insulin at intermediate ... chaperone activity of aB-crystallin against this fibril-forming protein, implying that it is the common positively charged group that plays a role in increasing the activity of aB-crystallin against ... chaperone activity of aB-crystallin against this fibril-forming target protein (Fig 4C) At 100 mm, Gdn-HCl had a negative effect on the chaperone activity of aB-crystallin in preventing amyloid...
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Tài liệu Báo cáo khoa học: The role of electrostatic interactions in the antitumor activity of dimeric RNases docx
Ngày tải lên :
19/02/2014, 06:20
... membranes, in particular of negatively charged membranes, in the mechanism of action of RNases, we investigated the ability of a series of RNases to destabilize model membranes as a representation of ... cytotoxic activity on eukaryotic and bacterial cells [32] Given the recently reported cytotoxic activity of the noncovalent dimers of RNase A [17], intriguing when compared to their sensitivity to ... Electrostatic interactions and antitumor RNases the dimer interface of the models or in the surroundings of the hinge loop residues Likewise, the models of nonexchanging and exchanging HHP-RNase...
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Tài liệu Báo cáo khoa học: ATPase activity of magnesium chelatase subunit I is required to maintain subunit D in vivo ppt
Ngày tải lên :
19/02/2014, 12:20
... conformational transition upon binding to BchH may bring the integrin I domain of BchD into contact with the integrin-binding motif of BchH, simultaneously triggering porphyrin metallation This would ... shown binding of Mg2+ to this subunit [21] An integrin I domain, suggested to exist in the C-terminus of the 70 kDa subunit, is a metal binding site (MIDAS motif) that can be expected to bind Mg2+ ... however, the lack of XAN-G has to be explained by an inhibited activity of the deficient XAN-H proteins Interestingly, the recombinant R capsulatus BchI proteins with exchanged amino acid residues...
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Tài liệu Báo cáo khoa học: Design, structure and biological activity of b-turn peptides of CD2 protein for inhibition of T-cell adhesion ppt
Ngày tải lên :
19/02/2014, 13:20
... directly interrupt the binding mechanism of CD2–CD58 Inhibition of cell adhesion involves interrupting the interaction of key residues at the protein– protein interface If we consider hydrogen bonding ... preliminary examination of biological activity of 12-mer linear and cyclic peptides, the peptides were truncated to six amino acid residues in order to elucidate the minimum number of critical amino ... CD2–CD58 interaction This correlates with the higher inhibitory activity of the cEL peptide and the very low inhibitory activity of the cYT peptide compared to other peptides The flanking residue of...
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Tài liệu Báo cáo khoa học: Inhibitory properties of cystatin F and its localization in U937 promonocyte cells ppt
Ngày tải lên :
19/02/2014, 17:20
... molar excess of papain The residual activity of papain was measured with Z-FR-AMC Relative activity of cystatin F is shown, from 0% (uninhibited enzyme) to 100% (the lowest activity of the enzyme) ... restore monomers and inhibitory activity under nondenaturing conditions Loss of inhibitory potential of dimerized cystatin F can be explained by blocking of the N-terminal part, disabling protease access, ... Dimerization of cystatin F leads to a loss of inhibitory activity (C) Immunoblot of cystatin F run on nondenaturing PAGE Cystatin F (200 nM) was incubated 15 at 37 C in phosphate buffer pH 6.0 using different...
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