dimerization apos s role in the mechanism of antimicrobial activity

... excess of CRI Monomers MSSAE and MSSG could not be tested as such for inhibition by CRI, because the strongly reducing conditions of the assay produce the cleavage of their mixed disuldes This in ... 3, show that inside the cells BS-RNase, MSSG and MSSAE are all present as dimers These dimers are covalent, as when the electrophoresis run was performed under reducing conditions, most of the ... to growing SVT2 cells 125Ilabelled MSSAE was detached by high salt from SVT2 cells at increasing time intervals In the insert, autoradiographic scans of the SDS/PAGE runs D and M mark the electrophoretic...

Ngày tải lên: 20/02/2014, 11:20

... sister She never behaves with respect to the other Just because she is her sister – except perhaps in those cases when her mother says, “Give in to her.” In the game of sisters playing at “sisters,” ... are sisters: they are dressed alike, they walk about holding hands – in short, they enact whatever emphasizes their relationship as sisters visa-vis adults and strangers The elder, holding the ... the other sister in the whole play situation.) Only actions that fit these rules are acceptable in the play situation In the game a situation is chosen that stresses the fact that these girls...

Ngày tải lên: 23/06/2014, 09:52

... lane 4), therefore confirming the interaction of these two proteins previously observed biochemically [37] or using a yeast two-hybrid assay [39] Interestingly, in the M2H system, the interaction ... Movies are presented at ten frames per second, representing a speed increase of ·40 Statistical analysis was performed using the graphpad prism, version (GraphPad Software, Inc., San Diego, CA, USA) ... with GRASP55 We next sought to define the nature of the GRASP55 binding site in the MT1-MMP ICD pACT plasmids driving the expression of MT1-MMP ICDs containing single-, double- and triple-point mutations...

Ngày tải lên: 18/02/2014, 04:20

... 58 As histidine residues are central to copper binding in many proteins they probably form part of the copper binding sites in this protein Although there are four histidines in the C-terminal, ... constituent of senile plaques of different disease origin [27] In the current study the ability of stefin B to bind copper was assessed We used isothermal titration Copper binds to cystatin B calorimetry ... function, specific to the cerebellum [18] It is located not only in the lysosomes and in the cytoplasm, but also in the nucleus [19] Lack of expression of stefin B is associated with signs of cerebellar...

Ngày tải lên: 19/02/2014, 06:20

... L-arabinose Methylation analysis demonstrated that a substantial amount of the L-arabinose residues (14%) in soy arabinogalactan is present as terminal residues [21], suggesting that of these arabinogalactans, ... of both linear and L-arabinose-substituted galactan side chains of pectin This is in agreement with a previous study in which the synergy of enzymes degrading the pectin side chains was studied ... genes encoding main chain cleaving enzymes as well as with the arabinanolytic genes [1] The absence of expression of galA in the presence of pectic galactan indicates that galactose is not an inducer...

Ngày tải lên: 21/02/2014, 01:21

... to assess the effects of the glycone moiety in this way as none of these compounds were substrates These data indicate that ¯avonoid-3-glycosides are not substrates for CBG DISCUSSION Fig Structures ... supernatant was estimated by activity measurement assays using 4NPGlc as substrate Expression of cbg-1 in P pastoris and isolation of reCBG Large-scale expression was achieved using 250 mL cultures in L ... determined by measuring the b-glucosidase activity in Ó FEBS 2002 50 mM sodium phosphate (pH range 2.8±7.6) The thermal stability of CBG was assessed by measuring the residual b-glucosidase activity...

Ngày tải lên: 08/03/2014, 16:20

... and (so far only identified in yeast) PEX17 Several other peroxins are involved in the subsequent steps of the import The import of matrix proteins seems to involve a cascade of interactions between ... molecular mass complex consists of TbPEX5 and TbPEX14-N,H as confirmed by SDS/PAGE analysis SDS/PAGE analyses of corresponding peak fractions confirmed the presence of both proteins in the shifted ... mislocalization of all three enzymes in these cells clearly shows the involvement of PEX14 in the targeting of PTS-1, PTS-2 and I-PTS proteins to the glycosome To further analyse the effect of...

Ngày tải lên: 23/03/2014, 17:21

... must satisfy restrictions of the null hypothesis, so the Wald statistics should be close to zero Table represents results for Wald tests of both hypotheses testing The p-value (p

Ngày tải lên: 30/03/2014, 05:20

... not shown) This suggests that the signaling pathway from the IFN-c receptor to the acyltransferase involves tyrosine kinases but not protein kinase C Such a finding is not unexpected as the Janus ... forms [27] Inhibition of precursor processing offers protection against a lethal dose of endotoxin [28] To assess whether an acyltransferase inhibitor affects transcription or the processing ... Resch, K (1998) The role of lysophosphatide acyltransferases and protein kinase C isoforms in the regulation of lymphocyte responses Biochem Soc Trans 26, 370–374 Jackson, S. K (1997) The role of...

Ngày tải lên: 31/03/2014, 01:20

... the submucosal protein expression of IL-1b, IL-6 and TNF There was no apparent submucosal staining in the vast majority of sections Occasional sections had positive staining in blood vessels and ... although the staining was not considered to be very strong cytokines in the intestines leading to greater tissue damage This study also demonstrated decreased proinflammatory cytokine levels in the intestines ... Gy G = 45 Gy There was no change in the level of expression at any time point Staining was only observed in the crypts, as indicated by the arrows There was no staining seen in the villi Ong...

Ngày tải lên: 09/08/2014, 08:22

... no staining in non-degenerate NP cells However, as in these previous studies, with degeneration, there was increased SA-βgal staining Because these discs (in our study) also showed shorter MTLs, ... particularly as p16INK4a may accumulate in response to specific forms of stress, including oxidative damage [18] Since the initial description of the pH-dependent staining of senescent fibroblasts by ... negative Senescence-associated β-galactosidase staining in cells directly extracted from human intervertebral disc tissue SA-β-gal staining was not observed in any of the NP cells isolated from the...

Ngày tải lên: 09/08/2014, 10:20

... the P1BS cis-acting element was present in the promoters of the genes SULTR1;3 and SULTR2;1, raising the possibility of the involvement of PHR1 in the crosstalk between sulfate and Pi signaling ... deficiency The pattern of expression of these genes in the pho1 mutant, with basal level of expression in Pi-sufficient roots but overexpression in Pi-deficient shoots, suggests that the response of these ... non-overlapping tissue-specific expression of miR395 and SULTR2;1 in roots [23] It is thus possible that the distinct response of SULTR2;1 and SULTR3;4 in roots and shoots of the phr1 mutant may also...

Ngày tải lên: 11/08/2014, 11:21

... mild asthma resulted in a significant increase in airway hyperresponsiveness that was associated with the elevation of sputum eosinophil numbers [17] A variety of findings suggest that atopic individuals ... on these cells T cells from asthmatic lungs show defective expression of Fas Co-culturing of T cells with IL-4 downregulates Fas expression on the cell surface The expression of IL-4 in the asthmatic ... the secondary lack of Fas expression might explain the persistence of inflammatory cellular infiltrates in allergic asthma Apoptosis of TH2 lymphocytes (and possibly eosinophils) could represent...

Ngày tải lên: 12/08/2014, 18:20

... bleeding principally from NVUGB - the usual setting as this group comprises 80% to 90% of all bleeders seen [28] Because of their effect on decreasing portal pressure, somatostatin and analogues ... stabilizes clot [11] and, possibly, accelerates healing of bleeding lesions over the 72 hours following endoscopic hemostasis [16,17] Somatostatin and octreotide inhibit acid, and decrease both pepsin ... endoscopy with possible banding The mainstay of diagnosis and mortality-improving treatment for patients with peptic ulcers at high risk of rebleeding remains early endoscopy Competing interests...

Ngày tải lên: 12/08/2014, 23:24

... hydrolysis reactions, carboxylesterases can increase the polarity of an exogenous substrate thus enhancing its elimination Exogenous substrates of carboxylesterases include angiotensin-converting ... carboxylesterases also contain four cysteine residues that are involved in disulfide bonds The carboxylesterase conserved catalytic site is comprised of a triad of the amino acid residues serine (Ser), ... CAUXIN, CES5 Figure Multi-tissue Northern blot analysis of human carboxylesterases: Distribution of carboxylesterases in human tissues was examined using a multi-tissue Northern Blot purchased...

Ngày tải lên: 24/08/2014, 12:18

... 30 minutes A list of core questions was used in the interviews: the subjects were asked to answer these questions at some point during the interview but no rigid structure was set and the subjects ... tasks, and they are often more interested in issues such as love, personal image or social standing than the mastery of school subjects Students motivation therefore lends itself to analysis ... matters to students, willingness to answer student s questions and quickness in marking and returning student s work after testing or collecting As for teacher s carefulness in lesson preparing,...

Ngày tải lên: 28/03/2015, 09:42

... attaching a His6 tag at the N-terminus of the expressed protein For analysis of the expressed protein, harvested cells were washed and lysed in SDS sample buffer The sample was treated with sonication ... Rho A [35] The results of those studies suggest that the lower eukaryotes and bacterial enzymes evolved as a separate lineage from the mammalian TGases The physiological roles of these invertebrate ... lane 2, 10 lg The arrow indicates the transcripts of PpTGase Mouse ribosomal RNA was used as a size marker Analyses of the recombinant and the plasmodial PpTGases were performed by SDS/PAGE on 7.5%...

Ngày tải lên: 17/03/2014, 23:20

... vessels, is mainly attributed to the presence of a lateral gradient in auxin concentration, auxin transport occurring preferentially towards the lower half of the bent stem This hypothesis is supported ... experiments showing that exogenous IAA induces or suppresses tension wood formation, when applied to the lower and upper sides of an inclined stem, respectively Reports concerning the intervention of ... of other phytohormones in this process are somewhat conflicting Several observations indicate that tension wood induction is a complex process The response to gravity, in terms of lateral auxin...

Ngày tải lên: 09/08/2014, 02:21

... seedlings [31] This suggests that the pattern of AtMYB60 expression in response to osmotic stress might be rather complex and dosedependent Even though the detailed analysis of the mechanisms ... expression domain of transcription factors, such as the MADS-box AGAMOUS gene [33] Most interestingly, seed-specific enhancers have been mapped in the intronic regions of seed-expressed genes in ... regulate the expression of the VvMYB60 gene extends beyond the scope of this work, it will be intriguing to further investigate the expression profile of VvMYB60 in different grape tissues in response...

Ngày tải lên: 11/08/2014, 11:21

... tyrosine kinases, cytokine receptors lack intrinsic enzymatic activities and need receptor-associated kinases to pass the signals downstream Janus kinases (JAKs) were identified in the early 9 0s, ... on their drug binding specificity The first class of PPIases, Cyclophilins, selectively bind to the immunosuppressive drug Cyclosporin A (CsA), and the second class, FK506-binding proteins (FKBPs), ... The signaling process involving STAT family proteins and its upstream kinase JAKs, the JAK-STAT pathway, is identified as one of the major cytokine signaling pathways, in which STATs are phosphorylated...

Ngày tải lên: 15/09/2015, 17:10