dig detection of pcr products
Cloning genes by PCR
Ngày tải lên :
25/10/2013, 22:20
... after Digest Ligation Figure 10.6 Schematic diagram showing a typical Southern blot of digested genomic DNA before and after ligation as part of IPCR The ‘Digest’ lane shows detection of a specific ... PROTOCOL PART C – PCR OF TAILED cDNA Add the following to a 0.5 or 0.2 ml PCR tube in a preheated heat block at 94°C: ● µl of 10 × PCR buffer; ● µl of 10 mM dNTP; ● 20 µM of GSP2; ● 20 µM of poly-G primer; ... time of is recommended and in case of long amplification products, a 3–4 extension time should be used for a total of 40 cycles For the secondround nested PCR, 1–10 µl of the first round PCR should...
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Báo cáo khoa học: The lactate dehydrogenases encoded by the ldh and ldhB genes in Lactococcus lactis exhibit distinct regulation and catalytic properties ) comparative modeling to probe the molecular basis pdf
Ngày tải lên :
16/03/2014, 05:20
... maximum at a pH of about 5.5 The pH dependence of this activation fitted well with a pKa of 6.3 ± 0.1 Lactacte dehydrogenases of Lactococcus lactis Fig Effect of pH on the activity of LDHB (d) and ... Examination of the model of the R state of LDH shows a much higher density of positive charge in the region of contact between the monomers than in LDHB, particularly in the region of contact ... carried out in the absence of Pi (Fig 2) At pH 7.0, the activity of LDHB was very low regardless of the presence of Pi; however, at lower pH values, the stimulatory effect of Pi increased progressively,...
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Genes and Autoimmunity - Intracellular Signaling and Microbiome Contribution by Spaska Angelova Stanilova pot
Ngày tải lên :
17/03/2014, 21:20
... surface of B cells and a subset of Treg cells [50] This suggested a function of FCRL3 in the regulation of autoimmunity, although its functions remain unknown Variants of the promoter of the Secretoglobin ... Spaska Angelova Stanilova, PhD, Dsc Professor of Molecular Biology and Immunology Head of Department of Molecular Biology, Immunology and Medical Genetics Faculty of Medicine, Trakia University Bulgaria ... involvement a: Limitation of motion in extremes of gaze b: Evident restriction of motion c: Fixation of a globe or globes V VI Corneal involvement 0: Absent a: Stippling of the cornea b: Ulceration...
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Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx
Ngày tải lên :
07/08/2014, 18:21
... total volume of 100 µl containing mM MgCl2, 0.2 mM each dNTPs, 20 pmol of each PCR primer, U of Taq DNA polymerase (Takara, Japan), and 10 µl of templates Temperature conditions consisted of an initial ... (7/1,682) 0.59 (10/1,682) 0.65 (11/1,682) a No of positive/No of samples examined No of negative/No of samples examined b Table Antibiotic resistance profiles of isolated E coli O157:H7 Antimicrobial ... described [14] Oligonucleotide sequence of primers and the predicted sizes of PCR amplified products are listed in Table Each primer pair Molecular determination of E coli O157:H7 had been determined...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
Ngày tải lên :
07/08/2014, 18:21
... the first round PCR consisted of 2.5 µl of 10X PCR buffer, 2.5 µl of 25 mM MgCl , µl of 2.5 mM deoxynucleoside triphosphate (dNTPs), 2.5 U of Taq-polymerase (Promega, USA), pmol of each primer, ... conserved regions of the 16S rRNA gene fragment (116 bp) of both Ehrlichia spp and Anaplasma spp were used The composition of PCR mix, reaction conditions and the sequence of PCR probe were essentially ... one cycle of extension at 72 C for For second round nested -PCR, pmol of each primer, HE1 and HE3 (Genotech, Korea) and µl of first PCR product as template DNA were included in the PCR mix described...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
Ngày tải lên :
09/08/2014, 10:23
... the role that this variety of intraarticular bacterial DNA plays in the pathogenesis of ReA and other forms of arthritides However, detection of bacterial DNAs in the ST of patients with arthritis ... increase the yield of PCR products [30-32] Pure DNA from either E coli or C trachomatis was used as a positive control for the broad-range PCR screening system Amplification products were visualized ... manipulation These included pipeting PCR components under a laminar flow of sterile air, using only sterile equipments, dedicated pre -PCR and post -PCR rooms, and dedicated sets of pipettes, disposable gloves,...
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Báo cáo y học: "Tetra-O-methyl nordihydroguaiaretic acid (Terameprocol) inhibits the NF-B-dependent transcription of TNF-a and MCP-1/CCL2 genes by preventing RelA from binding its cognate sites on DNA" ppsx
Ngày tải lên :
11/08/2014, 03:20
... analyzed per plate of experimental gene to avoid plate-to-plate variation Final RT -PCR data is expressed as the ratio of copy numbers of experimental gene per 103 or 104 copies of GAPDH for samples ... μg/ml of LPS or μg/ml of LPS and 25 μM TMP Following 24 h of treatment, supernatants were collected and levels of TNF-a (A) and MCP-1/ CCL2 (B) were determined by ELISA To assess the effects of ... to the backbone structure of NDGA [11] A number of these compounds have been examined for their antiviral activity For example, an analysis of eight methylated forms of NDGA for their ability...
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Báo cáo khoa học: " Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant" potx
Ngày tải lên :
12/08/2014, 18:22
... detecting of C chauvoei Detection threshold of the PCR method The detection thresholds for samples consisting of manure, soil, silage and biogas substrate before and after pasteurisation and digested ... present study where only 12% (3 out of 24) of the blood or muscle pieces were above the detection threshold of PCR, while 9% of the meat juice samples were above the detection threshold (Table 2) Inhibitory ... 44:175-178 Burtscher C, Wuertz S: Evaluation of the use of PCR and reverse transcriptase PCR for detection of pathogenic bacteria in biosolids from anaerobic digestor and aerobic composters Appl Environ...
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Báo cáo y học: "Wound healing and inflammation genes revealed by array analysis of ''''macrophageless'''' PU.1 null mice" ppt
Ngày tải lên :
14/08/2014, 14:21
... roles of hundreds of genes by the similarity of their temporal profile with genes whose function as part of the serum response cascade is well characterized [3,4] To overcome the problems of extended ... of genes have profiles that are largely independent of inflammation Genes in these clusters are expressed with similar profile whether wounds are in wild-type skin, where there is an influx of ... profiles, regardless of the levels of gene expression Nine clusters were identified in this way, and of these, seven correlated with clear episodes in the repair process The other two had profiles...
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Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
Ngày tải lên :
07/10/2015, 09:55
... Cytokine Profile Study using Real-Time PCR, TaqMan® Technology Real-Time PCR is a sensitive and specific means of quantifying a gene of interest It has the ability to monitor the progress of the PCR ... the number of cycles of PCR takes place, the higher the incidence of Taqman® probe binding and this in turn causes greater intensity of light emission cDNA Figure 19 TaqMan® Principles of First ... Cross tabulation of TSAB status in the strains of mice immunized with TSHR Table 20 51 61 Summary of cytokine profile and immunological markers in control and treated groups of Balb/c and Swiss...
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ESTROGENIC ACTIVITY LEVEL OF NAKDONG RIVER BASIN AND ITS CONTROL BY WATER TREATMENT PROCESSES
Ngày tải lên :
05/09/2013, 08:40
... downstream of Busan area of Nakdong river basin Estrogenic activity and concentration of estrogenic substances in the Nakdong River basin Relative estrogenic activity of the main and branch stream of ... reaction (min), v: volume of culture used in assay (ml), OD420: absorbance at the end of reaction, OD570: light scattering at the end of reaction, OD595: cell density at the start of the assay The results ... indicator for evaluating the performance of treatment processes, Proceeding of 2002 IWA World Water Congress, e20217a Kamata M., Evaluation of estrogenic activity of natural water by Yeast two-hybrid...
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Moment and Shear, Generated by DL on the Composite Section
Ngày tải lên :
29/09/2013, 12:20
... consists of a pair of 25k axles spaced 4ft apart Apply the dynamic load allowance to the tandem LRFD pre-stressed beam.mcd 7/1/2003 18 of 71 Load Combinations Combination 1: The effect of the design ... the effect of the design lane Combination 2: The effect of the design truck combined with the effect of the design lane Combination 3: For both the negative moment between points of contraflexure ... spans, and reaction at interior piers only 90% of two design trucks spaced a minimum of 50 ft between the lead axle of truck and the rear axle of truck 90% the design Lane The distance between...
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a discourse analysis of opening and closing speeches by masters of ceremonyon reality television showsin american english versus vietnames
Ngày tải lên :
26/11/2013, 12:41
... users of American English and Vietnamese as well as interpreters iii TABLE OF CONTENTS STATEMENT OF AUTHORSHIP i ABSTRACT ii LIST OF ABBREVIATIONS vi LIST OF TABLES ... substitution of a noun as head of a noun phrase by one or ones, or the substitution of a whole phrase by the same 15 - Verbal substitution is by means of “Do” “Do” operates as head of a verbal ... first part of something” by Oxford Advanced Learner’s Dictionary It also says closing is “coming at the end of a speech, a period of time or an activity” [24] The combination of the meaning of those...
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A RESEARCH ON ENGLISH INVERSION AND DIFFICULTIES ENCOUNTERED BY ENGLISH MAJORS OF HAI PHONG PRIVATE UNIVERSITY
Ngày tải lên :
11/12/2013, 23:48
... Thi Hong Hanh TABLE OF CONTENTS ACKNOWLEDGEMENTS SYMBOLS AND ABBREVIATIONS PART ONE: INTRODUCTION Rationale Aims of the study Methods of the study Scope of the study Design of the study PART TWO: ... orders in English 1.2 Definition of Inversion 1.3 Operators 1.4 Communicative classification of sentence types Summary of chapter one CHAPTER II: DIFFERENT TYPES OF INVERSION AND ITS EQUIVALENTS ... by one or more of these three criteria: a) The placing of the operator in front of subject: [24] Will John speak to the boss today? (Quirk.R, 1973:191) b) The initial positioning of an interrogative...
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A study on imperative and errors made by vietnamese learners
Ngày tải lên :
11/12/2013, 23:51
... Scope of the study Design of the study PART II: DEVELOPMENT Chapter 1: An overview of English mood Definition of mood Kinds of mood ... sincere thanks to all of you Hai Phong, Dinh Thi Phuong TABLE OF CONTENTS ACKNOWLEDGEMENT PART I: INTRODUCTION Rationale Aims of the study Methods of the study ... carried out my research paper Scope of the study Due to the limitation of time, I only focus on imperative mood and some errors that learners often make Due to lack of experience and knowledge, this...
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A study on subjuntive and errors made by vietnamese learners
Ngày tải lên :
11/12/2013, 23:53
... source of books These are methods I did my research IV – Scope of the study Because of the limitation of time and the range of study, I just concentrate on subjunctive mood – one kind of mood, ... to the association 3.2 Use of the past forms of the subjunctive mood Like the past forms of the subjunctive of the active voice, the past forms of the subjunctive of the passive voice are used ... past participle 3.1 Use of the present forms of the subjunctive mood Like the present subjunctive of the active voice, the present subjunctive of the passive voice is often used in subordinate...
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Tài liệu How to Win Friends and Influence People by Dale Carnegie docx
Ngày tải lên :
24/01/2014, 00:20
... grew as a child grows It grew and developed out of that laboratory, out of the experiences of thousands of adults Years ago, we started with a set of rules printed on a card no larger than a postcard ... his hat that night, walked into the town meeting, and blew off steam in public As a result of that talk of indignation, the citizens of Clifton, New Jersey, urged him to run for the town council ... speaker, the head of a large national association of food manufacturers, told how he had been unable to stand up and express his ideas at meetings of a board of directors As a result of learning to...
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