differentiated parental dna chain causes stem cell pattern of cell type switching in schizosaccharomyces pombe
Asymmetric cell division in the regulation of neural stem cell self renewel in drosophila melanogaster
Ngày tải lên :
11/09/2015, 14:32
... germline stem cell and its surrounding cap cells (in ovary) or hub cells (in testes) is a good example of how stem cells utilizing this mechanism establishes polarity and maintains its stem cell ... function in promoting and inhibiting stem cell self-renewal, loss of Pins function causes a reduction in neuroblast numbers in the larval brain due to occurrence of GMC/GMC siblings and in turn ... maintain a stem cell pool and to generate sufficient numbers of fully differentiated cells Defects in asymmetric stem cell division disrupt this 21 Introduction intricate equilibrium; very often...
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Báo cáo khoa học: " Linkage disequilibrium pattern of the ATM gene in breast cancer patients and controls; association of SNPs and haplotypes to radio-sensitivity and post-lumpectomy local recurrence" doc
Ngày tải lên :
09/08/2014, 10:21
... illustration of the protein with important areas such as substrate binding domains, Leucine zipper, ATP-binding domains, FAT domain and PI3K domain [17] together with exonic information (The size of the ... the domains of the protein described by [17], such http://www.ro-journal.com/content/2/1/25 as the PI3K domain, substrate binding domains and ATPbinding domains, is illustrated in Figure Single ... both in monomeric (active) and dimeric (inactive) state [16] The protein contains several important domains such as 1) the C-terminal protein kinase domain (PI3K-domain), 2) the substrate binding...
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Báo cáo y học: " Virological pattern of hepatitis B infection in an HIV-positive man with fatal fulminant hepatitis B: a case report" doc
Ngày tải lên :
11/08/2014, 17:21
... formation As RC -DNA genomes may have a competitive advantage over duplex linear (DL) -DNA genomes in initiating infection [11], our finding of nucleotide mutations in DR1 may indicate reduced ... first report of mixed HBV D/F genotype infection in plasma, and preC stop codon dominance in different compartments HBV genotype F was originally isolated in the Amerindian populations of the Americas ... of the liver, thus leading to the dominance of a welladapted variant in these sites of replication AA: amino acids; ALT: alanine aminotransferase; AP: alkaline phosphatase; AST: aspartate aminotransferase;...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... properties of derived cell types [19] Using genome-wide bisulfite sequencing (BS-seq) [21], we have recently determined the DNA methylation profile of the human embryonic stem cell line HSF1 [22] ... is being affected by specific changes in methylation of the binding site, instead of more general methylation changes in the surrounding region In these comparisons we matched the two cell lines ... splicing machinery is in any way regulated by the methylation of these sites, it is intriguing that this is yet one more piece of evidence indicating that splicing at chromatin are coupled with DNA...
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Tế bào gốc (Stem cell)
Ngày tải lên :
18/08/2012, 20:56
... thư tinh hoàn, đa u tủy, lơ-xê-mi, ung thư vú, u nguyên bào thần kinh, u lympho Non-Hodgkin, carcinoma tế bào thận, tái tạo tim sau đau tim, đái đường type I, tổn thương xương sụn, bệnh Parkinson… ... không tạo nên thể sinh vật hoàn chỉnh mà tạo nên tế bào, mô định Các tế bào gốc phôi lấy từ khối tế bào bên (inner cell mass) tế bào gốc vạn c/ Tế bào gốc đa (multipotent stem cells): Là tế bào ... loại tế bào gốc trưởng thành giai đoạn biệt hóa thấp c/ Tế bào gốc trưởng thành (Adult stem cells/Somatic stem cells): Còn gọi tế bào gốc thân Là tế bào chưa biệt hóa tìm thấy với số lượng mô người...
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Tài liệu Review of Chronic Graft- Versus-Host Disease in Children After Allogeneic Stem Cell Transplantation: Nursing Perspective pptx
Ngày tải lên :
12/02/2014, 19:20
... (Th2) cells can initiate a response against autoantigens leading to B -cell hyperre activity and production of autoantibodies, causing targetorgan damage, including skin, mucosa, eyes, joints, ... GVHD is initiated, T-cells produce additional proinflammatory cytokines, including tumor necrosis fac tor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-2, which in turn attract more T-cells ... Consensus recom mends a system for scoring chronic GVHD manifestations in organs and sites, including the skin, mouth, eyes, gastro intestinal tract, liver, lungs, joints and fascia, genital...
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Tài liệu INNOVATIONS IN STEM CELL TRANSPLANTATION doc
Ngày tải lên :
18/02/2014, 04:20
... blood cell or a brain cell During this last decade, the number of published articles or books investigating the role of stem cells in cell transplantation or regenerative medicine increased remarkably ... consist of two transmembrane glycoproteins, the α and β chains which are restricted to cells of the immune system (e.g B cells, dendritic cells - DCs), but can be induced by other cell types during ... the recent developments in the field of basic and clinical aspects of stem cells and cell transplantation Although we are close to finding pathways for stem cell therapies in many medical conditions,...
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Tài liệu New Advances in Stem Cell Transplantation Edited by Taner Demirer ppt
Ngày tải lên :
20/02/2014, 08:20
... factors including the key pluripotency regulator Oct4 Conserved RNA binding protein and stem cell marker Inhibitor of microRNA processing in embryonic stem (ES) and carcinoma (EC) cells Table Combinations ... procedures 5.1 Directprogramming of somatic cells into another cell type Switching from one somatic cell type into another cell type, not necessarily via a pluripotent cell state was first demonstrated ... or a brain cell Because of the unique abilities of stem cells, as opposed to a typical somatic cell, they are currently the target of ongoing research Research on stem cells is advancing in the...
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Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... each institution should maintain through its ESCRO committee a registry of hES cell lines in use and of investigators working in this field and descriptive information on the types of hES cell ... Banking of hES Cell Lines As hES cell research advances, it will be increasingly important for institutions that obtain, store, and use cell lines to have confidence in the value of stored cells, ... cells for the purpose of generating new hES cell lines, including the procurement of blastocysts in excess of clinical need from infertility clinics, blastocysts made through in vitro fertilization...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
Ngày tải lên :
07/03/2014, 00:20
... enhancement of IRES-dependent translation of p27kip1 in differentiated cells such as 293T cells [23], the protein level of p27kip1 in Ptb) ⁄ ) ES cells was not different from that in wild -type ES cells ... (A) Expression of cell cyclerelated proteins in Ptb) ⁄ ) ES cells Expression of p27, cyclin A, cyclin B and cyclin E was examined by western blotting Whole cell extracts from wild -type, heterozygous ... Polypyrimidine tract-binding protein promotes insulin secretory granule biogenesis Nat Cell Biol 6, 207–214 Kosinski PA, Laughlin J, Singh K & Covey LR (2003) A complex containing polypyrimidine tract-binding...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
Ngày tải lên :
16/03/2014, 18:20
... fluorescence measured in q0 cells was lower than that in the parental cell lines containing mtDNA The decrease was substantial in lung (90%) and muscle (40%) cells but was less evident in bone (less ... normally in the absence of mtDNA These findings are in line with results found in hepatoma-derived Hep1q0 cells [16] ROS When DCF signal was assessed as a direct index of ROS, all of the q0 cells ... proteins causing half-maximal inhibition of the rate of NBT reduction GST activity was assayed in the supernatant of cell lysates, as described [39] Briefly, 150 lg protein were incubated in 50...
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Autoimmune Diseases – Contributing Factors, Specific Cases of Autoimmune Diseases, and Stem Cell and Other Therapies pdf
Ngày tải lên :
16/03/2014, 21:20
... 50–69% insulin-positive area taken as starting point and 0% insulin-positive area as end-stage (In' t Veld 2011) In all stages, CD8+ T cells are predominant, increasing in number with decreasing insulin-positive ... of clinical presentation, insulitis, a chronic inflammatory infiltrate of the islets affecting primarily insulin containing islets, is present in the majority of cases The mononuclear cell infiltrates ... becoming the most prominent infiltrating cell type in insulin-deficient islets Human T1D Involvement in CD4+ CD8+ the NOD mouse autoantibodies T cells T cells autoantigen Expression Subcellular...
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Commercializing the stem cell sciences pot
Ngày tải lên :
20/03/2014, 06:20
... successfully developing with this model, including Stem Cells Inc (http://www stemcellsic.com), Cellartis (http://www.cellartis.com) and Cellular Dynamics International (http://www.cellulardynamics.com) ... been in routine use in clinical medicine ever since, often providing a source of disease-specific cells or normal cells to test drug reactions Human embryonic, adult and induced pluripotent cell ... role of innovation systems in the commercialization of biotechnology 3.1 The international context of stem cell science 55 58 3.1.1 Innovating for enhanced competitiveness 60 3.1.2 The principle...
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NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) ppt
Ngày tải lên :
20/03/2014, 09:21
... characteristics of mesenchymal stem cells in cancer immunotherapy in mice Research results show that the mesenchymal stem cells injected into mice at the dose of 107 cells/ml can inhibit the proliferation of ... cell- based therapy: a new paradigm in regenerative medicine, J Cell Mol Med 13, 2009 STEM CELL RESEARCH USING MESENCHYMAL (MESENCHYMAL STEM CELL - MSC) IN THE TREATMENT OF CANCER AGENCY - RECTUM RATS ... Therapeutic potential of mesenchymal stem cells producing interferonalpha in a mouse melanoma lung metastasis model, Stem Cells 26, (2008), 2332-8 Satija, N K et al., Mesenchymal stem cell- based therapy:...
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Báo cáo khoa học: A transcription factor of lipid synthesis, sterol regulatory element-binding protein (SREBP)-1a causes G1 cell-cycle arrest after accumulation of cyclin-dependent kinase (cdk) inhibitors pdf
Ngày tải lên :
23/03/2014, 07:20
... Sato R (1998) Characterization of two Chinese hamster ovary cell lines expressing the COOH-terminal domains of sterol regulatory element-binding protein (SREBP)-1 Cell Struct Funct 23, 187–192 ... explaining the p27 protein elevation (Fig 4A,D) The data show that SREBP-1a regulates an assortment of genes involved in the control of cell proliferation On induction of exogenous SREBP-1a protein ... of endogenous SREBP-1 on cell growth To determine the physiological relevance of the growth-inhibitory action of SREBP-1a, the role of endogenous SREBP-1a in cell proliferation was examined in...
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Expression pattern of matrix metalloproteinases in human gynecological cancer cell lines ppt
Ngày tải lên :
28/03/2014, 14:20
... other MMPs in at least one of the cell lines For MMP-8, -12 and -21 only very weak mRNA expression could be observed in single cell lines Nine MMPs, which were present in most of the cell lines, were ... expression pattern concerning endometrial cancer cell lines In addition, MMP-3, -10 and -13 were expressed in cervical carcinoma and teratocarcinoma cell lines only Caski and PA-1 cell lines could ... performed in independent triplicates Western blotting For protein extraction, 106 cells were lysed in precooled Ripa-buffer (Pierce, Rockford, Ilinois) containing phosphatase inhibitors (Phosphatase Inhibitor...
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stem cell biology - daniel r. marshak
Ngày tải lên :
08/04/2014, 12:53
... generation of DSB in some cells, but not in other related cells, is the key to defining the program of switching in cell lineage Should the observed pattern of switching in mitotically dividing cells ... General Issues Introduction: Stem Cell Biology, Daniel R Marshak, David Gottlieb, and Richard L Gardner Differentiated Parental DNA Chain Causes Stem Cell Pattern of Cell- type Switching in Schizosaccharomyces ... Orientation of DNA replication establishes matingtype switching pattern in S pombe Nature 400: 181–184 ——— 2000 swi1 and swi3 perform imprinting, pausing, and termination of DNA replication in S pombe Cell...
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hematopoietic stem cell protocols
Ngày tải lên :
11/04/2014, 09:45
... unenriched cells will be sorted, complete the staining by incubating in streptavidin conjugated to Texas Red or PharRed for 15–20 on ice After washing, resuspend the cells in staining medium containing ... not included in the lineage cocktail After incubating for 20 min, wash the cells twice by diluting in staining medium followed by centrifugation The cells can now either be pre-enriched using ... sorting of individual cells produces cell suspensions of very high purity (>99%) However, FACS is a relatively slow method of isolating cells capable of sorting 2–50 × 103 cells/s (1.2–30 × 105 cells/min)...
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university of california press fundamentals of the stem cell debate the scientific religious ethical and political issues dec 2007
Ngày tải lên :
11/06/2014, 12:52
... the same cells and in different cells (D) These cells, grown from the neural retina, show staining common to brain neural stem cells (DCX, red) and staining found only in neural stem cells derived ... Doublecortin (DCX, red), vimentin (green), and nestin (blue) staining in a neurosphere demonstrate the intimate commingling of the cells in a sphere as well as expression of multiple markers both in ... determinants during division of a neural stem cell in a fly embryo, showing the opposite localizations for ACD determinants in the stem cell and differentiating cell The Miranda protein, stained red,...
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