differentially expressed proteins identified by proteomic analysis

... identify differentially expressed genes Effect of gene-specific knockdown on the accumulation of BaMV To investigate the roles of the differentially expressed genes identified by cDNA-AFLP analysis ... http://www.biomedcentral.com/1471-2229/10/286 Page of 12 Table Transcript-derived fragments identified by cDNA-AFLP analysis and differentially expressed between Mockand Bamboo mosaic virus-inoculated Nicotiana benthamiana ... Additional material Additional file 1: Table S1: Transcript-derived fragments identified by cDNA-AFLP analysis and differentially expressed between Mockand Bamboo mosaic virus-inoculated Nicotiana benthamiana...

Ngày tải lên: 11/08/2014, 11:21

... designed by the Ethics Committee and signed by the patients involved in the study before the trial All the patients understood the trial’s purpose and procedures Proteomics Proteomics analysis, ... present study, 31 proteins were identified as differentially expressed among normal colorectal mucosa, nmCRC and mCRC To some extent, this result is consistent with data reported by other groups ... that are free of contaminating serum proteins, red blood cells, connective tissue and necrotic tissue [10] The 31 spots representing differentially expressed proteins among normal colorectal mucosa,...

Ngày tải lên: 23/03/2014, 03:20

... the 2,067 total differentially expressed genes identified, 127 genes were found to be differentially expressed in both tissues, 115 of which were consistently differentially expressed, i.e., ... are expressed in male gametes of plants [10], it is not surprising to see much few differentially expressed (DE) genes were identified when anthers were used Because the Se gene appears to be expressed ... be differentially expressed in the semigametic genotype 57-4 compared to its non-semigametic isogenic line Pima S-1 (Additional file and 2) Of the 284 differentially Page of expressed genes identified...

Ngày tải lên: 11/08/2014, 11:21

... here on proteins, and sets of proteins, having evidence of a case-control difference in plasma concentration for CHD or stroke, and on the overlap of these proteins with those altered by Ealone ... spectrometry (MS/MS) analysis Protein identification and case versus control concentration assessment The acquired liquid chromatography MS/MS data were processed by a Computational Proteomics Analysis System ... disease, groups that contained proteins that were targeted for depletion, and groups in which all proteins had been annotated as ‘defunct’ by IPI, were excluded from analysis Page of 13 quantified...

Ngày tải lên: 11/08/2014, 12:20

... cellular proteins by Western blot Following the identification of cellular proteins by proteomic method, immunoblot analysis was carried out to confirm their presence In Western blot analysis, ... sixty one host proteins were successfully identified and categorized into eight different groups as follows: cytoskeleton proteins, stress Table Structural proteins of PRRSV identified by gel slice ... purified PRRSV virions by CsCl gradients combined with sucrose gradients ultracentrifugation Virion-associated proteins were identified by using 2-DE/MS proteomics approach followed by Western blot...

Ngày tải lên: 12/08/2014, 01:22

... doi:10.1186/ar2937 Cite this article as: Sugino H, et al.: DNA microarray analysis of rheumatoid arthritis susceptibility genes identified by genome-wide association studies Arthritis Research & Therapy ... CTLA4 123890 16380915 No change 0.767 STAT4 600558 17804842 Up 1.38 x 10–8 a P values determined by comparison between 112 rheumatoid arthritis patients and 45 healthy individuals Abbreviations ... studies; RA, rheumatoid arthritis Competing interests This study was financially supported partly by the grant from the Ministry of Health, Labor and Welfare of Japan Author details Graduate School...

Ngày tải lên: 12/08/2014, 11:23

... blood cell membrane proteins include: (a) integral proteins, (b) peripheral proteins and (c) glycosylphosphatidylinositol (GPI) anchored proteins Integral proteins Integral proteins comprise up ... of identified proteins from AARBC membrane pellet fraction 116 Classification of identified proteins from FNRBC membrane pellet fraction 134 Table 5.6 Comparison of AARBC and FNRBC membrane proteins ... information on the membrane proteins of AARBCs In this thesis, I explore the possibility of using a proteomics strategy to identify membrane proteins differentially expressed on adult anucleate...

Ngày tải lên: 09/10/2015, 11:06

... expression of apoptosis-related proteins by hypoxia in the JAR cell line demonstrated using the PowerBlot Western array We used a proteomic method to identify hypoxia-regulated proteins The PowerBlot ... proapoptotic and antiapoptotic proteins were detected PowerBlot analysis showed altered expression of many proteins involved in apoptosis, including the expression profiles of proteins involved in the ... not by regulation of the JNK gene, but by modulating phosphorylation of JNKs Thus, Bag-1 may inhibit apoptosis by suppressing the expression of Bid and Bad Bag-1 may also enhance apoptosis by...

Ngày tải lên: 31/10/2012, 15:12

... Step by Step Analysis • Introduction to tcpdump • Writing tcpdump Filters • Examination of Datagram Fields • Beginning Analysis • Real World Examples • Step by Step Analysis This page ... set of fragments with fragment ID 28843 The first fragment is 1480 bytes and the offset is byte The second fragment starts at byte 1480 and has a length of 1480; this is normal Finally, the third ... to 65,535 How many bytes are needed to represent the maximum value of 65,535? 216 -1 represents two bit fields or bytes for each port Minimally, you can see we need at least bytes in the UDP header...

Ngày tải lên: 10/12/2013, 16:15

... 2010 FEBS 4911 Proteomics of a PD model T Alberio et al Table Identification of differentially expressed proteins Protein spots in silver-stained gels were analyzed by ANOVA DA, proteins that showed ... Proteomics analysis reveals quantitative changes in 23 proteins Proteomic investigations were conducted on b-gal and a-syn cells treated or not with dopamine, as described above Statistical analysis, ... subunit by comparison with 2D electrophoresis (2-DE) maps available in the SWISS 2D-PAGE database (http://www.expasy.org) Additionally, 21 differentially expressed proteins were identified by LC-MS-MS...

Ngày tải lên: 15/02/2014, 01:20

... were differentially expressed in our study Some genes were differentially expressed in both tissues, showing increased apoptotic ⁄ autophagic activity Among these genes, MTA-1 was strongly expressed ... days 3–7, followed by a decrease at day 14, and then a smaller increase at days 17–24 at 25 °C compared with controls (13 °C) (Fig 2A and Table 1) The most differentially expressed transcripts ... lethal values [22] Under mild thermal stress at 25 °C, genes coding for antistress proteins were differentially expressed Protection against cellular stress, inflammation and stimulation of immune...

Ngày tải lên: 18/02/2014, 16:20

... proteins according to their cellular localization (Fig 3A) reveals that the majority of these proteins are integral membrane proteins (27%), membrane-associated proteins (21%), or cytosolic proteins ... encode 48 proteins that are specifically recognized by the UIM domain of Hrs (Fig and Table 1) The specific binding of the identified proteins to the Hrs UIM domain suggests that these proteins may ... between two proteins in vitro [23], rather than indirect analysis of the interactions between fusion proteins inside the yeast nucleus Moreover, our IVEC screening approach complements other proteomic...

Ngày tải lên: 07/03/2014, 03:20

... DCV-injected flies was analyzed by HPLC and the molecule induced by the viral infection was detected by MALDI-TOF MS (Fig 2B) The molecule was purified to homogeneity by gel permeation and reversed-phase ... pathway) mutant flies were infected by septic injury, and expression of phk genes was analyzed by Northern blot (C) phk-3 Expression is upregulated by LPS (+) and repressed by the molting hormone ecdysone ... induced by septic injury Phk-2 is induced by DCV infection, whereas Phk-3 is induced by bacterial challenge The third member of this family in Drosophila, Phk-1, is not induced and is specifically expressed...

Ngày tải lên: 08/03/2014, 08:20

... starvation by addition of Dulbecco’s modified Eagle’s medium/10% fetal bovine serum The cells were harvested at different time points after serum addition Synchronization was confirmed by FACS analysis ... (Promega) by introducing an AvrII-site and thereby abolishing the initiating ATG, generating pGL2-Avr A fragment encoding for the first eight codons of E2F6 and partial 5¢UTR was amplified by PCR ... region (5¢UTR) (A) Identification of the E2F6 transcriptional start site by primer extension analysis Primer extension analysis was performed with radiolabelled primer SG50 (right lane) A genomic...

Ngày tải lên: 08/03/2014, 09:20

... at 405 nm RESULTS BIAcore analysis of rhIGF1R binding by IGF analogues High af®nity binding of IGF-I, IGF-II and their analogues to rhIGF1R was measured by BIAcore analysis (Figs and 2), and ... BIAcore analysis of IGF-II and IGF-II analogue binding to highanity recombinant IGF1R Binding of IGF-II (A), des-(1±6)-IGF-II (B) and Arg6-IGF-II (C) to rhIGF1R measured by BIAcore analysis ... DISCUSSION We have made a direct comparison of rhIGF1R binding by IGF-I, IGF-II and 14 analogues using BIAcore analysis IGF1R binding by all of these analogues has not previously been compared in...

Ngày tải lên: 08/03/2014, 22:20

... also to be regulated by light in leaves Aspartic proteinase genes have been shown to be induced by wounding in tomato leaves [39], by senescence in daylily petals [7] and by low osmoticum in B ... gene specific, we examined whether the AtPasp A1 gene was regulated by light We isolated leaf The three genes are differentially expressed Although, we have not detected the AtPasp A3 protein, ESTs ... and A2 proteins have overall 81% identity while the protein derived from A3 gene is 63 and 64% identical to the predicted A1 and A2 proteins, respectively Unsurprisingly, the predicted proteins...

Ngày tải lên: 17/03/2014, 11:20

... enhanced by adding a secondary task because the Timed Up and Go Test time w.962s(Go)-G te6 Falls and walking under dual-task in elderly subjects Each subject was interviewed successively by two ... following tests in this order First, a single leg balance test with eyes open followed by eyes closed, and second, gait analysis in single- and dual-task conditions were analysed 271 Tests Single leg ... disturbed by the dual task, whereas those of the nonfallers were not impact Thus, the results of Bowen et al.32 cannot be extended to the normally healthy population It has also been suggested by Bronstein...

Ngày tải lên: 28/03/2014, 20:20

... p53 proteins mutated at specific phosphorylation sites expressed in insect cells by using PAb240 as a reference control for total p53 protein Western blot analyses of murine full-length p53 proteins ... in which proteins are expressed and post-translationally modified [8] Experimental procedures Baculoviral expression and purification of p53 proteins S37D S376A S376D S378A S378D Fig p53 proteins ... Recombinant human and murine wild-type p53 proteins are phosphorylated at S15 and acetylated at K382 when expressed in insect cells Western blot analyses of purified p53 proteins are shown, using PAb240...

Ngày tải lên: 30/03/2014, 16:20

... greater The stabilization of wild-type StP by a half-saturating concentration of phosphate can be expressed quantitatively by a dramatic up-shift in Cm value by 4.0 M, compared to the control reaction ... stability brought about by the mutations is reflected by significant shifts of the Cm values for the mutants, relative to that for the wild-type, to higher urea concentrations by % M or greater The ... was precipitated with mL of M NaCl and removed by centrifugation at 13 000 g The DNA in the supernatant was precipitated with ethanol and purified by standard protocols [16] From a comparison of...

Ngày tải lên: 31/03/2014, 01:20

... publications For each of the microarray studies, if a gene was identified by the original authors using their criteria as differentially expressed after TGFβ stimulation at any of the time points ... http://www.translational-medicine.com/content/7/1/12 Figure set) Differentially expressed genes in the two subgroups of glioblastomas with strong and weak TGFβ response (in the Freije dataDifferentially expressed genes in the two ... anti-TGFβ therapies are likely to be beneficial for those patients By examining the genes differentially expressed between the two identified subgroups of glioblastomas that showed different TGFβ...

Ngày tải lên: 18/06/2014, 15:20