different+immunometric+assays+for+the+detection+of+pth

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... Moreover, to assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

Ngày tải lên: 03/11/2012, 10:58

... supported by the research project The research on the development of the total evaluation technique for the hazardous impacts by the chemical substances towards human and ecology (the project ... and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts in isolated rat hepatocytes treated with 6-nitrochrysene, ... postlabeling method, etc.) Therefore, it is important to develop a simple rapid in vitro method for evaluating the generation of reactive oxygen species from a variety of genotoxic chemicals We...

Ngày tải lên: 05/09/2013, 08:40

... respectively One of the weaknesses of this study is the small sample size In addition, the majority of the lesions examined appear to be of the Class I type, rather than Class II lesions for which the technology ... appropriate to the purpose of the test? Use examiners representative of the target or primary clinical providers of the test? Show evidence that the test can distinguish cancer/precancer from other conditions ... predictive value Therefore, the lack of apparent control as well as documentation with respect to the manner in which the sample was selected limits the utility of this study Poate et al performed a...

Ngày tải lên: 06/03/2014, 02:21

... drops of the creatine solution, three drops of the ethanolic solution of 1-naphthol and then two drops of the potassium hydroxide solution; shake after the addition of each reagent The formation of ... incubation for 24 h ± h, examine the plates (9.4.3) for the presence of typical colonies of Salmonella and atypical colonies that may be Salmonella (see Note) Mark their position on the bottom of the ... the results of the interpretation, indicate the presence or absence of Salmonella in a test portion of x g or x ml of product (see ISO 7218) See annex C for the precision data obtained from the...

Ngày tải lên: 07/03/2014, 16:20

... the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the literature [18] ... one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to NO2 that are responsible for the high sensitivity associated with the promotion However, whether ... and was then diffracted onto the detector The detector collected data for all angles in the range 4±648, 2y, simultaneously Fig gives the diffraction spectra of the WO3 before and after the NO...

Ngày tải lên: 19/03/2014, 16:47

... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... between the 833 bp of the ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87% nucleotide identity The 834 bp sequence of the ... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses...

Ngày tải lên: 18/06/2014, 22:20

... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... between the 833 bp of the ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87% nucleotide identity The 834 bp sequence of the ... to those in the RRV sequence, and highlight the similarity of the primer sequences within the RV2 lineage of rhadinoviruses and the dissimilarity with members of the RV1 lineage of rhadinoviruses...

Ngày tải lên: 20/06/2014, 04:20

... adipogenic differentiated hMSCs, also in the corner areas of the microcavity (Figure 3f) The performed studies verify the biocompatibility of the Si3N4 membrane and the suitability of the microcavity for ... between the individual cell responses Analysis of the biocompatibility of the MCC Figure The miniaturized cell culture chamber (a) Work flow of the fabrication (b) Design of the MCC The MCC contains ... Parkinson disease, for the development of new sensitive drug screening methods and for the quantification of toxicodynamic and toxicokinetic effects Application of the MCC for the analysis of nanoparticleinduced...

Ngày tải lên: 21/06/2014, 00:20

... (12) then corresponds to the correlation of the real part, z1c(nT), of the SMF’s output, y1c(nT), with the known real symbols, an, over the known signal duration B Performance The performance of ... detectors as the phase diversity between the sources increases We still note the best performance of the completely informed detectors (C1 and O1) and the lowest performance of the less informed detectors ... statistical theory of detection, the optimal receiver for the detection of the K symbols an from both the K primary data xv(nT) and the K’ secondary data bTv(nT)’ consists in comparing to a threshold the...

Ngày tải lên: 21/06/2014, 00:20

... Page of adjust the pH of the mixture to 9.1 under stirring The NaHTe solution prepared in the first step was added to the reaction mixture under N protection The resultant mixture was stirred for ... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis of the cutoff value Detection of ... Page of predenatured at 95°C for 10 min, then 55°C for 30 The particles coupled with streptavidin were added into the hybridization mixtures and incubated at 37°C for 10 and enriched in the bottom...

Ngày tải lên: 21/06/2014, 01:20

... probability of detection close to 85% could be achieved for a probability of false alarm 10% in the case of vocal fold paralysis in male utterances, while the probability of detection for vocal ... probability of detection for vocal fold paralysis in men was measured, that is approximately equal to 90% for probability of false alarm 10% The gain in the probability of detection for vocal fold ... probability of detection approximately 73% for probability of false alarm 4% [33] For the same probability of false alarm, a probability of detection, which falls between 80.95% for rectangular...

Ngày tải lên: 21/06/2014, 20:20

... selectivity when they are used just for Zn(II) detection On the other hand, the fluorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... intracellular environment for the detection of Zn(II) References ð1Þ Wherein, A is the value of the fluorescence intensity, C is the concentration of Zn(II), and the range of C is from 0.2 lM to ... happened in the temperature range from 200 °C to 450 °C due to the degradation of the GNQ grafted to the MWNTs The content of the GNQ grafted to the MWNTs is about 12 wt%, which is similar to the calculation...

Ngày tải lên: 22/06/2014, 01:20

... corresponds to the maximal value in the images, the intensity value of the vertical bright stripes therefore always equals 255, which is the maximal possible value as the depth of all the considered ... of a widely used real-time industrial application The core component of the algorithm is the selection and the extraction of the features best describing the image contents The first step of the ... illuminations must be used for covering the complete circumference of a metallic cylinder The number NC of needed cameras depends not only on the diameter of the object DO , the width of the ray aperture’s...

Ngày tải lên: 22/06/2014, 19:20

... Comparison of Diagnostic Methods for the Detection of Parasites in Fish al., 1998; Anderson, 1999) Our primer sets were designed for identification of different flukes and they were useful for detection ... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods for the Detection of Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods for the Detection of Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the...

Ngày tải lên: 06/08/2014, 19:20

... recommended for diagnosis of CHV in formalin-fixed tissues The optimized ISH assay, which developed in this study, may be a valid tool for the study of pathogenesis and diagnosis of CHV infection ... hybridization for CHV In this study, several ISH protocols, which were consisted of the changes of enzyme-concentrations, the time of hybridization and hybridization probes, were compared in formalin ... practicality of digoxigenin-labeled probe is better than those of biotin-labeled probe in the hybridization assay for the detection of CHV These results suggest that ISH assay using digoxigenin-labeled...

Ngày tải lên: 07/08/2014, 17:22

... RT-PCR for the detection of Japanese encephalitis virus 349 Fig Sensitivity of real-time RT-PCR assay for quantitative detection of JEV (A) and detection of JEV by RT-PCR (B) One milliliter of JEV ... and 5' NTR didn’t Therefore, primers and probe of 3' NTR for the diagnostic purpose of JEV were selected The concentration of primers and probe giving the highest fluorescent and the lowest threshold ... Comparison of real-time RT-PCR and gel electrophoresis analysis of the product for the detection of JEV RNA Three different primers and probes were designed based on conserved regions of 5' NTR,...

Ngày tải lên: 07/08/2014, 18:20

... monitor the development of the antibody level using the yolk diluted 1,000 times with PBST The yolk antibody also showed no cross reaction to any other bacteria (Table 1), and the antibody levels of ... combinations at 7,000 rpm for 30 After centrifugation at 14,000 g for 40 of the supernatant from previous centrifugation, the flagella of pellet were resuspended in PBS and stored at 20oC The protein concentrations ... buffer (pH 9.6) to the concentration of 10 µg/ml and 100 µg of diluted MAb was added into ELISA plate (Costar, Netherlands) The plate was incubated for h at 37oC and overnight at 4oC The wells were...

Ngày tải lên: 07/08/2014, 18:21

... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... elbaliava gnisu dengised erew stegrat lla rof seborp dna sremirp RCP-TR* emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5 ... eborp/remirp redael fo gningised eht rof ylno detcelfer saw noiger B2 laitrap ot redaeL morf gnirevoc ,revewoh ,niarts 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat...

Ngày tải lên: 07/08/2014, 18:21

... etis-A eht ni egnahc lanoitamrofnoc lacol a secudni gnidnib nicymomoraP DJ isilguP ,S awazihsoY ,D ymruoF 054-344 ,36 ,9791 scirtaideP yduts pu-wollof dellortnoc raey-ruof a fo stluser :nicymanak ... ta nim 03 rof detabucni erew setalp eht ,yltneuqesbuS llew hcae ot dedda saw noitulos etartsbus )]dica cinoflus enilozaihtzneb lyhte-3[id-oniza-2,2( STBA lµ 001 ,gnihsaw retfA h rof esadixorep ... ydobitna gnicudorp llec amodirbyh elbats A tnatanrepus erutluc gnisu sllec gnicudorp-ydobitna rof neercs ot demrofrep saw ASILE tceridni evititepmoc ,noisuf eht retfa syad evlewT ]8[ ylsuoiverp debircsed...

Ngày tải lên: 07/08/2014, 18:21

... cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( STBA etartsbus eht fo emulov lacitnedi na ,h rof Co73 ta noitabucni retfA ... rof yllacipocsorcim denimaxe saw llew hcaE syad rof 2OC %5 rednu ,Co73 ta detabucni erew setalp ehT llew/sllec 000,02 fo tnuoc llec lanif a ot erutxim eht ot dedda erew sllec 401FT eht ,h rof ... emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ares detavitcani eht htiw tset NS ehT evitisop...

Ngày tải lên: 07/08/2014, 18:21