diagnostic and prognostic immunohistochemistry of dipeptidyl peptidase iv

... (2002) Detection of the activity of the ectopeptidases DPIV and APN using sensitive fluorogenic substrates In Ectopeptidases: CD13/Aminopeptidase N and CD26/Dipeptidylpeptidase IV in 27 28 29 30 ... Tat(1–9) and Asp709 as well as Asp739 of DP IV, between the C-terminal Glu9 of Tat(1–9) and the side chains of Arg560 and Lys554 of DP IV Furthermore, the side chain of Asp5 of Tat(1–9) was DP IV cleaves ... carbonyl group of Val4 of the peptide and the backbone amide hydrogen of Ala743 of the enzyme, between the side chain carbonyl group of Asn7 of Tat(1–9) and the side chain of Lys554 of DP IV and finally,...

Ngày tải lên: 20/02/2014, 11:20

... Sensitivity, specificity, and positive and negative predictive values (PPVs and NPVs) of the ELISA tests were calculated To analyze anti-CFFCP and anti-CCP2 status with relevant quantitative variables, ... study of diagnostic value were included in a prospective study of prognostic factors in early RA Two years of follow-up data were available for 98 of 134 patients Results for radiographic and clinical ... some RA sera Positive results with CFFCP1, CFFCP2, and CFFCP3 were observed in 14.3%, 27.4%, and 9.5% of sera from anti-CCP2-negative patients, respectively, and antiCCP2-positive status was observed...

Ngày tải lên: 09/08/2014, 14:22

... human liver fibrosis severity [6] Dipeptidyl peptidase (DP8) and dipeptidyl peptidase (DP9) are recently cloned proteinases of the DPIV gene family DP8 and DP9 are closely related peptidases of 61% ... (2002) Prolonged survival and decreased Functions of dipeptidyl peptidases, DP8 and DP9 15 16 17 18 19 20 21 22 23 24 25 26 27 invasive activity attributable to dipeptidyl peptidase IV overexpression ... Council of Australia project grant 142607 to GWM and MDG and PhD scholarships to DMTY and XMW References Gorrell MD (2005) Dipeptidyl peptidase IV and related enzymes in cell biology and liver disorders...

Ngày tải lên: 23/03/2014, 11:20

... concentrations of IL-12 and IL-15 in sera of RA patients are increased independently of disease activity, while blood plasma DPPIV activity correlates inversely and T-cell DPPIV activity/CD26 expression ... independent of antigenic reactivity or, conversely, where CD26 antigenic activity but not DPPIV activity was measured This is complicated further by a lack of understanding of the breadth of substrate and ... Ohhashi M, Nagatsu T: Activities of dipeptidyl peptidase II and dipeptidyl peptidase IV in mice with lupus erythematosus-like syndrome and in patients with lupus erythematosus and rheumatoid arthritis...

Ngày tải lên: 09/08/2014, 07:20

... perception and nociception, is inactivated by DPIV enzyme activity [77] DPIV enzyme activity is effective on a number of chemokines in vitro (Table 2) DPIV in cell biology A number of DPIV-binding ... S9B Dipeptidyl peptidase IV (DPIV) S9C Acylaminoacyl peptidase S9D Glutamyl endopeptidase (plant) S9B subclan - DPIV gene family DPIV Fibroblast activation protein (FAP) DP8 DP9 Nonenzyme DPIV-related ... acinar cells of mucous and salivary glands and pancreas; by the uterus; and by immune organs such as thymus, spleen and lymph node [22–25] (Fig 2) Our recent study using the DPIV selective inhibitor,...

Ngày tải lên: 06/03/2014, 09:22

... epitope in both native and denatured rDPP IV, independent of DPP IV enzymatic activity, which is suggestive of a noncompetitive inhibition mechanism The 6A3 epitope in full-length rDPP IV was finely ... structures of DPP -IV (CD26) from rat kidney exhibit flexible accommodation of peptidase- selective inhibitors Biochemistry (Mosc) 45, 7474–7482 24 Gorrell MD (2005) Dipeptidyl peptidase IV and related ... species-specificity of 6A3-recognition of DPP IV in immunoprecipitation and in immunoblotting assays We found that 6A3 strongly recognized rDPP IV but only negligibly bound to native human (h)DPP IV and mouse...

Ngày tải lên: 29/03/2014, 22:21

... ng/ml cutoff point, PCT had a sensitivity of 85% and a specificity of 95% in predicting infection, whereas CRP had a sensitivity of only 64% and a specificity of 84% at a cutoff level of 150 mg/l ... al [35] evaluated the diagnostic value of PCT and CRP in a group of 59 patients undergoing CPB At a cutoff level of ng/ml, PCT had a sensitivity of 86% and a specificity of 98% in predicting infection, ... Peak PCT level of 7.7 ng/ml had a sensitivity of 100% and a specificity of 100% in predicting organ failure Peak PCT level of ng/ml had a sensitivity of 100% and a specificity of 65% in predicting...

Ngày tải lên: 13/08/2014, 03:20

... a-helices and a final b-strand, F, which protrudes from the core of the monomer and runs anti-parallel to the same strand of a second monomer, allowing for the formation of the dimer The surface of interaction ... a-helices Five b-strands, labelled from A to E, run all contiguous The N-terminus of the monomer includes strand A, an a-helix and a long stretch, and the C-terminal portion includes two relatively ... functional cagPAI, is the activation of nuclear transcription factor-jB and the induction of pro-inflammatory production of cytokines, mainly interleukin (IL)-8 [12] The activation of a pro-inflammatory...

Ngày tải lên: 06/03/2014, 00:21

... indicating the loss of a total of 4844 E T Anderson et al (Eur J Biochem 269) Ó FEBS 2002 Proteolytic activity and thermal stability of the C5a peptidase Fig Incubation of S512A C5a peptidase precursor ... region of C5a peptidase Cleavage of the pro-sequence peptide and maturation of C5a peptidase precursor is realized via an intramolecular autoprocessing mechanism Alternatively, processing of C5a peptidase ... exhibits characteristic positive band at 194 nm and negative bands at 210 nm and 215 nm Heating of C5a peptidase up to 98 °C abolished these features (Fig 5D inset) Monitoring of the ellipticity at...

Ngày tải lên: 08/03/2014, 16:20

... frequency of liver disease, suggesting the influence of alcoholism These results correspond to those of Alvarez et al (6) and Van den Brande (15) but contrast with those of Katz et al (17) and Umeki ... importance of sputum AFB smear/ culture for the diagnosis of TB in the elderly A positive sputum AFB smear was obtained in 57.1% of elderly and in 57.5 % of young TB patients, and a positive AFB ... limit), Fernandez-Villar et al (30) found an 18.2% incidence of hepatotoxicity in the risk factor group (advanced age, chronic liver disease, abuse of alcohol, and others), and van den Brande et...

Ngày tải lên: 15/03/2014, 03:20

... AAS and MJ are recipients of junior and senior research fellowships from Council of Scientific and Industrial Research, Government of India, and University Grants Commission, New Delhi, respectively ... formation of shift (Fig 7B; lanes and 7) This result suggests that shift was created by binding a heterodimer of Ets-1 and Elk-1 to the probe, and the N-terminus of Ets-1 and the C-terminus of Elk-1 ... C ⁄ EBP and Sp1 (Fig 1), and removal of this region results in a 40% decrease in promoter activity Fig Elevation of DPP-III mRNA, promoter activity and pElk-1 levels by H-ras in human liver cells...

Ngày tải lên: 29/03/2014, 09:20

... as 18 peptidases for efficient hydrolysis of the imported peptides [9] Of these peptidases, four are proline-specific: proline iminopeptidase, prolinase, X-prolyl dipeptidyl aminopeptidase and prolidase ... relationships of prolidase, hence providing a greater understanding of the characteristics of this peptidase, which would be of industrial use in the debittering of fermented foods Results and Discussion ... unit of prolidase activity is defined as hydrolysis of lmol of peptide in FEBS Journal 275 (2008) 271–280 ª 2007 The Authors Journal compilation ª 2007 FEBS 273 S I Yang and T Tanaka Relative activity...

Ngày tải lên: 30/03/2014, 04:20

... skeletal/soft-tissue injury, limb ischemia, shock and age (Figure 1) Shock and age are rated with a score of 0-2 each, skeletal/softtissue injury with a score of 1-4 and limb ischemia with a score of ... conception and design of the study, acquisition of data, analysis and interpretation of data, drafting the article and revising of the article for important intellectual content All authors have read and ... http://www.josr-online.com/content/5/1/25 Page of great challenge in terms of diagnostics, operative treatment and perioperative management Furthermore, the question of limb salvage in children with vascular...

Ngày tải lên: 20/06/2014, 04:20

... from São Paulo and Espírito Santo States are composed by, respectively: 67.2% and 42.2% Caucasian; 6.2% and 8.5% Afrodescendant; 25.4% and 48.6% Brown (Pardo) and 1.3% and 0.7% Native Brazilians ... at stage IV, in a total of 89 staged cancers We have also observed a slight predominance of poor staged cancers, as 43.8% of cases had a better prognosis profile (I and II) versus 56.2% of the ... FIGO-staging profile has Page of 10 also been documented in other studies performed in Brazil: Badiglian Filho et al [27] have reported an incidence of 26.3% and 42%, and Derchain et al [32] of 34% and...

Ngày tải lên: 20/06/2014, 08:20

... Positive vs negative 47 vs 42 26 vs 63 0.022 0.116 MAGE-A3/4 Positive vs negative 24 vs 65 0.009 NY-ESO-1 Positive vs negative 19 vs 70 0.068 CTA positive with vs without 52 vs 37 0.001 CTA positive ... expression and overall survival The correlation of clinicopathological parameters and individual or combined CTA expression with overall survival was further investigated As shown in Table 3, univariate ... expression Positive (n = 51) P value Down-regulated (n = 38) MAGE-A1 Positive 18 33 30 Positive 11 13 Negative 40 25 Negative MAGE-A3/4 0.144 0.184 NY-ESO-1 11 Negative Table Expression of cancer-testis...

Ngày tải lên: 10/08/2014, 10:20

... positive and negative predictive values, and accuracy (defined as the sum of concordant cells divided by the sum of all cells in a × table) and their 95% CIs were calculated Comparisons of these diagnostic ... 0.41 (95% CI 0.34 to 0.48), a positive predictive value of 0.30 (95% CI 0.24 to 0.38) and a negative predictive value of 0.92 (95% CI 0.83 to 0.96) The accuracy of PCT was found to be 0.50 (95% ... cutoff value of 0.2 μg/l exhibited a sensitivity of 0.77 and a specificity of 0.59 for identifying patients with bacterial/ parasitic infection, and its efficiency was comparable to that of the...

Ngày tải lên: 13/08/2014, 03:21

... clinicians and/ or validated clinical severity scores Conceptually, the likelihood of an adverse outcome should determine the medical indication, the length of hospitalization and the allocation of healthcare ... secretagogue of the hypothalamo– pituitary–adrenal axis to produce adrenocorticotropic hormone and cortisol Serum cortisol levels have been reported to be proportionate to the degree of stress and, by ... disturbances 11 Of course, any biomarkers will always oversimplify the interpretation of important variables, and therefore biomarkers are meant to complement, rather than to supersede, the judgment of clinicians...

Ngày tải lên: 13/08/2014, 10:20

... 0.981) and the optimal cutoff value was 1020.5 pg/ml (Figure 2a) The BNP levels at admission representing a negative predictive value of 100% and a positive predictive value of 100% were 115.0 and ... design of the study and revised the manuscript critically YIH, HRK, SHJ, DGK and YRS participated in the design and coordination of the study YAB reviewed chest radiography KSJ conceived of the ... The medical records of the patients were reviewed, and the following information was recorded: age, gender, aetiology of CKD, prior history of congestive heart failure (CHF) and coronary artery...

Ngày tải lên: 13/08/2014, 16:20

... subgroup of conduct disordered adolescents identified as callous unemotional [29] Intermittent explosive disorder (IED) is characterized by impulsive aggressive and assaultive behaviors out of proportion ... maintained attitude of ill-will [18-20] Problems with Irritability in the DSM A Depression 1) Irritable mood was ill-conceived as a DSM diagnostic definer of dysthymia and major depressive disorder ... context, increased motor activity, an expansive/elated commonly delusional outlook, decreased sleep, and often also by grandiose ideation, paranoia, dysphoria, distractibility, and irritability/anger...

Ngày tải lên: 13/08/2014, 18:21

... functions of ghrelin by correlations with a wide number of markers of inflammation, organ dysfunction and metabolism and to examine potential protective effects of ghrelin in critically ill patients and ... recording of intensive care treatment, such as volume therapy, vasopressor infusions, demand of ventilation and ventilation hours, antibiotic and antimycotic therapy, renal replacement therapy and ... liver disease [23] To test the impact of the underlying etiology of critical illness we performed extensive subgroup analysis Therefore, nonsepsis patients were divided into liver cirrhosis and...

Ngày tải lên: 13/08/2014, 20:22