diabetes in vitro study

Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Ngày tải lên : 25/10/2012, 11:10

... the loading of pravastatin into erythrocytes was directly proportional with increase of drug concentration in the incubation medium, in the 2-10 mg/ml concentration range This finding is in concurrence ... endocytosis, including vinblastine, chlorpromazine, hydrocortisone, propranolol, tetracaine, retinol, and primaquine [16, 19] The current work aims to study the encapsulation of pravastatin in human carrier ... pravastatin However, the relative impacts of different in vitro findings on the overall in vivo drug delivery efficacy of these cellular carriers remain to be evaluated during the future in vivo...

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Báo cáo khoa học: Human skin cell stress response to GSM-900 mobile phone signals In vitro study on isolated primary cells and reconstructed epidermis docx

Ngày tải lên : 07/03/2014, 11:20

... possible induction of cell stress in the skin by WÆkg)1 GSM-900 exposure for 48 h No apoptosis was induced in either skin cell type, in agreement with reports of other in vitro studies concluding ... mJÆcm2 single dose, h post exposure) Scale bar: 25 lm Human reconstructed epidermis GSM-900 did not induce an in ammatory process in hRE In these experiments using haematoxylin ⁄ eosin-stained reconstructed ... expression (Fig 6J) In the case of Hsp70 (Fig 6G–I), instead of being expressed only in the cytoplasm as in NHDFe, it was also expressed in the nucleus UVB exposure induced a slight increase in Hsp70 expression,...

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báo cáo hóa học:" Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study" docx

Ngày tải lên : 20/06/2014, 01:20

... protein was steadily expressed in fibrin/PLGA and PLGA Interestingly, suppression of collagen type I was observed in fibrin/PLGA and PLGA at weeks and weeks β-actin gene was steadily expressed in ... participated in the design of the study and conceived of the study RBHI participated in the design of the study and conceived of the study GK participated in the design of the study, conceived the study ... of in vitro conMeasurement of cell proliferation activity of in vitro constructs Fibrin/PLGA and PLGA construct exhibited similar growth pattern in vitro Cells proliferation was gradually increased...

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báo cáo hóa học:" The effect of newer anti-rheumatic drugs on osteogenic cell proliferation: an in-vitro study" doc

Ngày tải lên : 20/06/2014, 01:20

... cells remaining in the intra-trabecular spaces and plated at fragment per well in a 24-well plate containing ml of control medium (α-MEM containing 1% Gentamicin v/v and 10% foetal bovine serum) ... increasing the action of the first and negating that of the second That drugs can interact is not unexpected Systematically investigating such interactions in vitro is slowly becoming a routine ... guidelines has been established in some reviews[17,18] In light of these many uncertainties, we carried out an invitro study of the effect of various antirheumatoid drugs alone and in combination...

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Báo cáo khoa học: "Contribution of the xenograft bone plate-screw system in lumbar transpedicular stabilization of dogs: an in-vitro study" docx

Ngày tải lên : 07/08/2014, 20:23

... Harper KD, Nightingale RW The role of imaging and In Situ biomechanical testing in assessing pedicle screw pull-out strength Spine 1996, 21, 1962-1968 Sandén B, Olerud C, Petrén-Mallmin M, Larsson ... and clinical application of these systems [6] In addition, there have been no answers with the detailed information on which instrument is the best choice in spinal surgery All the systems include ... loosening and instability The load values were recorded in mm intervals up to 16 mm; using the moment-force relation, the forces were converted to the moment with using the radian angle definition,...

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Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot

Ngày tải lên : 09/08/2014, 01:22

... and 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer ... all time points Alizarin red staining was performed to assess mineralisation, and no positive staining was observed either in the MSCs or in native tissue during the culture period investigated...

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Báo cáo y học: "Differential direct effects of cyclo-oxygenase-1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitro study" pptx

Ngày tải lên : 09/08/2014, 07:20

... Therefore it remains important to extend these in vitro studies with animal in vivo studies and even clinical setups Conclusion Although they are in vitro findings, the results of the present study suggest ... to those of indomethacin, indicating that inhibition of COX-1 results in an adverse effect on proteoglycan synthesis and retention In contrast, when COX-2 is selectively inhibited in OA cartilage ... seen within the context of the significant anti-inflammatory effects of these NSAIDs By inhibiting joint inflammation, they may indirectly be beneficial to cartilage, specifically when inflammation...

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Báo cáo y học: "Exogenous sphingomyelinase increases collagen and sulphated glycosaminoglycan production by primary articular chondrocytes: an in vitro study" pps

Ngày tải lên : 09/08/2014, 08:22

... protein kinase PKR plays a pivotal role in cartilage homeostasis [8], we inhibited PKR activity to determine whether PKR is involved in the observed changes in matrix synthesis In control cells, inhibition ... dose-dependent increase in cell death with a concomitant decrease in cell number This is in accordance with the known role for ceramide in initiating a cellular stress response resulting in cell death ... This in accordance with studies in human keratinocytes, which have shown that a rapid (15 minutes) but transient (returning to baseline by hour) increase in endogenous ceramide occurs following...

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Báo cáo y học: "Key regulatory molecules of cartilage destruction in rheumatoid arthritis: an in vitro study" docx

Ngày tải lên : 09/08/2014, 10:22

... several genes involved in the NF-κB signaling pathway showed differential expression, including interleukin-1 receptor antagonist (IL1RN), receptor-interacting serine/threonine kinase (RIPK2), ... IGFBP-3, = insulin-like growth factor binding protein-3, 4; IL-1α, 1β, 6, = interleukin-1α, 1β, 6, 8; IL-1ra = interleukin-1 receptor antagonist; MCP-1 = monocyte chemoattractant protein-1; MIP-1α, ... (TLR2), spermine synthase (SMS), thioredoxin interacting protein (TXNIP) and BCL2-related protein A1 (BCL2A1) Apart from NF-κB-associated genes, some cytokines/chemokines and receptors were induced,...

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Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc

Ngày tải lên : 11/08/2014, 08:21

... [17] In comparison with NF161, NF177 demonstrated stronger analgesic activity in the writhing test in mice, remarkably lower anti-inflammatory activity in the carrageenininduced paw edema assay in ... activity in leukocytes and ECs CAM activity can be regulated by modulating their expression or intrinsic adhesive functions [18] PMNs maintain several of these receptors in an inactive state during ... heat-inactivated bovine calf serum (BCS), 100 U/ml penicillin, 100 µg/ml streptomycin, U.I./ml heparin, 12 µg/ml bovine brain extract and 200 mM glutamine HUVEC were grown to confluence in flasks...

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In vitro study of surface functionalization of titanium substrates for potential enhancement of osseointegration and reduction of bacterial infection

Ngày tải lên : 10/09/2015, 09:13

... usually has many binding sites (e.g many amino or carboxylic groups in one molecule), and immobilization may interfere with the ligand binding domain or transform the molecule into an unfavorable ... osseointegration (Williams, 2008) Implants can be integrated in host bone by mechanical fit such as using a screw in a predrilled hole or impacting the prosthesis into a pre-prepared cavity in ... successful bone healing For example, fibronectin and vitronectin can interact with the integrins on mesenchymal stem cells (MSCs) and enhance their attachment on the implant surface, and fibrinogen, von...

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In vivo and in vitro study on potential combination therapy of COL 3 and tamoxifen in breast cancer a pilot study

Ngày tải lên : 09/10/2015, 11:24

... Chapter In Vitro Studies 5.1 PROTEIN BINDING STUDY 5.1.1 Introduction The binding of drugs to plasma and tissue proteins is a major determinant of drug distribution throughout the body Protein binding ... COL-3 bind mainly to albumin So protein-binding interaction, where competition for the protein-biding site involves, could be a good reason to explain this phenomenon Some cases of protein displacement ... protein binding of acenocoumarol was reduced at higher concertations of E7070[153] However, in the present study, in vitro protein binding of COL-3 was not altered by tamoxifen, suggesting that interactions...

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Remineralization of early enamel caries lesions using different bioactive elements containing toothpastes an in vitro study pdf

Ngày tải lên : 16/09/2016, 17:14

... combination of arginine and calcium carbonate favors calcium and phosphate ions to deposit dentin-like minerals within dentin tubules and on the dentin surface This combination works on the principle ... within dentin tubules and on the dentin surface The in vitro study was designed to evaluate the remineralization potential of various commercial toothpastes that contain different active remineralization ... surface in the ACC and CSP groups (Figs 3b, 3c) Discussion 168 The present study demonstrates that Colgate Sensitive Pro-Relief and NovaMin R are superior in promoting remineralization and inhibiting...

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báo cáo hóa học: " A refined in vitro model to study inflammatory responses in organotypic membrane culture of postnatal rat hippocampal slices" potx

Ngày tải lên : 19/06/2014, 22:20

... of in vitro hippocampal slice culture as an intermediate model between single cell lines and in vivo models Furthermore, we wanted to examine the possibility of refining the model by taking into ... (n = in each group) preclinical in vivo experiments Therefore, we also wanted to study the possibility of refining the model itself by taking into the account the experimental design involving ... reliable, defined model to be used in preclinical drug studies involving immune reactions and inflammation Competing interests The author(s) declare that they have no competing interests Page...

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Báo cáo khoa học: "An ultrastructural study on cytotoxic effects of mono(2-ethylhexyl) phthalate (MEHP) on testes in Shiba goat in vitro" pptx

Ngày tải lên : 07/08/2014, 18:20

... Araldite-M Semithin sections of µm were stained with 0.5% toluidine blue for light microscopy Ultrathin sections were stained with uranyl acetate and lead citrate, and examined in a JEM-1200 EX ... showed marginated chromatins along the nuclear membrane, ruptured vesicles within the MNB, and some swollen and ruptured cell organelles, e.g mitochondria Discussion In this study, the in vitro model ... pathologic findings, Sertoli nuclei frequently showed a slight margination of chromatin, especially at the electron microscope level [18] The peculiar morphological change obtained in this study was...

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báo cáo khoa học: " A cluster randomized trial to improve adherence to evidence-based guidelines on diabetes and reduce clinical inertia in primary care physicians in Belgium: study protocol [NTR 1369]" potx

Ngày tải lên : 11/08/2014, 16:21

... opinion leaders and experts in diabetes care [41] Modeling phase In the modeling phase, we delineated the components of our complex intervention and the underlying mechanisms by which they influence ... registry, clinician reminders, facilitated relay of clinical information to clinicians, patient reminder systems, and continuous quality improvement Five interventions are not included in the service ... self-monitoring of blood glucose levels Level two: professional Clinician education Interventions for clinician education include an increased understanding of principles guiding clinical care...

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Báo cáo khoa học: "Comparative study on the in vitro and in vivo properties of two bovine herpesvirus-5 reference strains" pptx

Ngày tải lên : 12/08/2014, 18:22

... caused by infectious bovine rhinotracheitis virus in calves in Hungary Acta Vet Acad Sci Hung 1969, 19:145-151 Moretti B, Orfei Z, Mondino G, Persechino A: Infectious bovine rhinotracheitis, clinical ... been found circulating during the last years [22] As suggested by our in vitro results, A663 strain could have diminished capacities to be maintained in the cattle population, in contrast with ... sulfolipocyclodextrin in a squalane -in- water and water -in- mineral oil emulsions for BHV-1 vaccines in cattle Vaccine 2000, 19:132-41 32 Reed CJ, Muench HA: A simple method of estimating fifty per cent end points...

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Báo cáo y học: "The triple combination of tenofovir, emtricitabine and efavirenz shows synergistic anti-HIV-1 activity in vitro: a mechanism of action study" pdf

Ngày tải lên : 12/08/2014, 23:21

... FTC-MP-terminated DNA/RT/TFV-DP to test the hypothesis that the incoming NRTI might act as the next nucleotide to the chain-terminated primer and form a DEC, thus stabilizing the preexisting chain-termination ... studied in parallel As shown in Fig 5a and Table 4, TFVterminated DNA was able to form DEC with RT in the presence of dCTP or FTC-TP Interestingly, TFV-terminated DNA+RT formed DEC with incoming ... ddCMP-terminated DNA in the presence of EFV, with the FTC-MPterminated DNA forming complexes 4.8- to 31-fold less In this study, NRTI+NRTI and NRTI+NNRTI drug combinations were investigated using cell-based...

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Báo cáo y học: " Sodium nitroprusside and peroxynitrite effect on hepatic DNases: an in vitro and in vivo study" potx

Ngày tải lên : 13/08/2014, 13:20

... changes monitored by using Beckman DU 530 spectrophotometer Protein carbonyls and protein nitrotyrosine were measured in plasma proteins and the remaining protein pellet according to the method of ... assisted during in vivo and in vitro experiments and did the graphical presentation DS performed statistical analysis and assisted during in vivo experiments DP and RK assisted during in vitro experiments ... endonuclease activity in the culture of neuronal cells In our in vivo study (Table 2), the activity of alkaline DNase increased within 24 h after exposure to SNP (achieving approximately a similar...

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Báo cáo sinh học: " In-vitro model systems to study Hepatitis C Virus" ppt

Ngày tải lên : 14/08/2014, 19:22

... life and contamination problems in primary hepatocytes, scientists tried to develop immortalized human hepatoma cell lines Many cell lines supported HCV infection and replication in- vitro such as ... detected in NS3, NS5A and NS5B proteins [22,24] Adaptive mutation in NS5A region causes interferon resistance The highly adapted 5.1 replicon contained three adaptive mutations (two in NS3 and one in ... failed to initiate productive HCV infection With the passage of time, new molecular techniques made it possible to develop an efficient in- vitro culture system to facilitate the study of HCV Initial...

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