dfi values of human sperm samples from two commercial scsa ® laboratories
Báo cáo y học: "Flow cytometric assessment of the signaling status of human B lymphocytes from normal and autoimmune individuals" doc
Ngày tải lên :
09/08/2014, 01:23
... analysis of cellular signaling status Importantly, temperature control of samples from the harvest to the time of the experiment must be monitored carefully Often, the temperature of the samples ... small number of primary B cells from human patients This technique will allow assessment of kinase activation in situations where cell numbers are limiting and/or the number of samples is great, ... κ Assessment of MAPK and NF-κB activation in peripheral B cells from normal individuals or SLE patients To compare MAPK and NF-κB activation in human B cells from the periphery of SLE patients...
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Báo cáo y học: " Analysis of the copy number profiles of several tumor samples from the same patient reveals the successive steps in tumorigenesis" ppt
Ngày tải lên :
09/08/2014, 20:22
... values drawn from a normal distribution with a mean of and a standard deviation of S Simulations were carried out for values of C from to 0.8 (80% of normal cells in the sample), and values of ... profile of the common precursor of two samples At each step of the algorithm, the profile of the common precursor of the two nodes joined, CP(a i ,a j ), is inferred This profile is reconstructed chromosome ... frequency Fk of each breakpoint is calculated from a reference data set of independent samples (b) An identical breakpoint score (IBS), characterizing the similarity of two profiles in terms of chromosome...
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báo cáo khoa học: " A qualitative exploration of travel-related risk behaviours of injection drug users from two Slovene regions" pps
Ngày tải lên :
11/08/2014, 18:20
... part of a study of HIV infection risks Of the sample, 47% (96/204) reported travel experiences in the previous ten years Drug injecting, safety of sex, number of sexual partners and duration of ... Republiki Sloveniji (In English: Report of the drug situation 2009 of the Republic of Slovenia) Edited by: Krek M Public Health Institute of the Republic of Slovenia Ljubljana; 2010 Trautmann F, ... exploration of travelrelated risk behaviours of injection drug users from two Slovene regions Harm Reduction Journal 2011 8:8 Submit your next manuscript to BioMed Central and take full advantage of: ...
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Báo cáo y học: "Analysis of infectious virus clones from two HIV-1 superinfection cases suggests that the primary strains have lower fitnesc" ppt
Ngày tải lên :
13/08/2014, 01:20
... Expression profiles were analysed with the GCNPro™ (Gene Network Central) software [68] Clustering of the gene expression profiles induced by the HIV-1 clones is shown for a selection of genes from a ... edu/BioEdit/bioedit.html] 58 MEGA software package [http://www.megasoftware.net] 59 Tamura K, Nei M: Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees ... fitness of clone B2.5 was slightly lower than that of clone B2.3, but higher than that of the B1 strains The ranking order of relative fitness is: B2.3 ≥ B2.5 > B1.1 ≥ B1.2 >> B1.3 The outcome of...
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Báo cáo y học: "Contamination of human DNA samples with mouse DNA can lead to false detection of XMRV-like sequences" docx
Ngày tải lên :
13/08/2014, 01:20
... high proportion of DNA samples from the healthy volunteers (19/36), but only 2/112 of the CFS patients, yielded PCR products of the correct size, as tested on an agarose gel None of the “no template” ... DNA Figure Sensitivity of nested PCR for XMRV gag A) Titration of genomic DNA from WPI-1282 PCR amplicons from 83.3, 13.8, 2.3, 0.3, 0.05 and cell equivalents of genomic DNA from the WPI-1282 cell ... laboratory strains of inbred mice [2] None of the samples from either CFS patients or healthy controls was positive in this assay, although we were able to detect a signal from two XMRV-infected...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
Ngày tải lên :
13/08/2014, 23:20
... promise of human ESC seen against the destruction of human embryos required to realize such aspirations, compelling arguments have been articulated both in support of and in opposition to human ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these ... been the only source for human ESCs The fact that live human embryos must be destroyed to produce human ESCs presents a substantial ethical obstacle for the advancement of human ESC research With...
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Báo cáo y học: "Computational prediction of human metabolic pathways from the complete human genome" ppt
Ngày tải lên :
14/08/2014, 14:21
... clusters of pathways The average length of HumanCyc pathways is 5.4 reaction steps Example HumanCyc pathways are shown in Figures and All HumanCyc pathways can be accessed online from the HumanCyc ... shows statistics from version 7.5 of HumanCyc (released in August 2003), after manual refinement of the PGDB was completed The 2,742 enzyme genes in HumanCyc correspond to 9.5% of the human genome, ... Our best estimate of the total number of human metabolic enzymes is the sum of the 1,653 known enzymes plus the 203 pathway holes, for a total of approximately 6.5% of the human genome allocated...
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Báo cáo y học: "Systematic bioinformatic analysis of expression levels of 17,330 human genes across 9,783 samples from 175 types of healthy and pathological tissues" ppt
Ngày tải lên :
14/08/2014, 21:20
... parameters were a mean of and standard deviation of This step of the sample-wise normalization was necessary to prevent a small number of aberrant samples from dominating the mean values for genes ... to 0.15 and that of the tissues jumped from 0.22 to 0.92 (Additional data file 5) Third, correlation of data from two large datasets where the same samples had been analyzed on two different array ... used data for 14 samples of human muscle biopsy samples from patients with inflammatory myopathies [9] For these cases, data from hybridizations on both HG-U95Av2 and HG-U133A human arrays were...
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Computational fluid dynamics analysis of a twisted airfoil shaped two-bladed H-Darrieus rotor made from fibreglass reinforced plastic (FRP)
Ngày tải lên :
05/09/2013, 15:28
... the convergence of the solution, the torque co-efficient (Ct) values are calculated for each value of input air velocity and rotor rotational speed and from the values of Ct, Cp values are obtained ... Variation of Ct with TSR, and (b) deviation of computational Ct from experimental Cp for H/D ratio 0.85 (a) (b) Figure (a) Variation of Cp with TSR, and (b) deviation of computational Cp from experimental ... Variation of Cp with TSR, and (b) deviation of computational Cp from experimental Cp for H/D ratio 1.10 (a) (b) Figure 11 (a) Variation of Ct with TSR, and (b) deviation of computational Ct from experimental...
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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
Ngày tải lên :
19/02/2014, 06:20
... fragments of different sizes were amplified from the RNA of QGP-1 cells and pancreas (Fig 2A) Determination of the nucleotide sequences of the RT-PCR products revealed A Confirmation of utilization of ... to include only the 5¢-UTR of the mRNA [15,16] However, comparison of the sequence of the 5¢-UTR of human pancreatic DNASE1 mRNA reported previously [15] with the human genomic sequence showed ... treat patients with stable angina pectoris, offers an in vivo model of mild myocardial ischemia in humans Irrespective of a lack of alteration in levels of other conventional cardiac markers such...
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Tài liệu Báo cáo khoa học: Two novel variants of human medium chain acyl-CoA dehydrogenase (MCAD) K364R, a folding mutation, and R256T, a catalytic-site mutation resulting in a well-folded but totally inactive protein pptx
Ngày tải lên :
20/02/2014, 02:21
... in one of the two defective alleles [5] Symptoms of MCAD deficiency cover a broad spectrum, ranging from hypoglycaemia to seizures, coma and sudden death, and usually present at a time of metabolic ... biochemical outcome of these point mutations Results The effect of chaperonin on the ability of the mutant proteins to fold For a number of other MCAD point mutations, co-overexpression of the GroELS ... standard deviation of the mean result) (B) Western blot analysis of native PAGE gels, indicative of relative amounts of soluble tetramer No MCAD activity was detected in lysates of cells expressing...
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Human Capital and the Development of Financial Institutions: Evidence from Thailand docx
Ngày tải lên :
06/03/2014, 10:20
... Characteristics of Village Banks # of Village Banks % of banks which offer savings % of banks which offer lending % of banks which offer savings and lending Median Years of Operation Median # of Members ... effects of the years of schooling of the head of the household, the years of schooling of the money manager and the average schooling of the heads of the village households are estimated All of the ... years of schooling of village heads of household Figures 2, and describe the relationship between the same bank policies and the years of schooling of the village bank’s money manager All of the...
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Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
Ngày tải lên :
08/03/2014, 22:20
... Identification of two sites of sulfation of human heparin cofactor II J Biol Chem 261, 15827–15830 10 Kim, Y.-S., Lee, K.-B & Linhardt, R.J (1988) Microheterogeneity of plasma glycoproteins heparin cofactor ... : mixture during the presence of the sulfation inhibitor Purification of HCII from plasma and from recombinant CHO cells HCII from outdated citrated human plasma of a single blood donor was isolated ... cofactor II: N-glycans and sulfation (Eur J Biochem 269) 985 Fig MALDI-TOF mass spectra of the reduced and permethylated total N-glycans enzymatically liberated from human HCII isolated (A) from...
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Comparative Study of HIV Associated Pulmonary Tuberculosis in Chest Clinics from Two Regions of Edo State, Nigeria docx
Ngày tải lên :
22/03/2014, 18:20
... level of PTB in Benin (28.6%) than their counterparts in Irrua (0%, P < 0.05), there is however no significant difference in the incidence of HIV and PTB by occupation of subjects from the two ... HIV-PTB co-infection rate of 5.2% from Lagos State, while Onipede et al.,18 reported 12.9% from Ile-Ife, Ogun State Okogun et al.,19 also reported a prevalence rate of 5.3% from Abeokuta and environs ... clinics from two regions of Edo States (Benin environs and Irrua environs), it should be noted that there is no significant difference in the incidence of HIV, PTB and HIV-PTB in the two regions...
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Support to the identification of potential risks for the environment and human health arising from hydrocarbons operations involving hydraulic fracturing in Europe doc
Ngày tải lên :
23/03/2014, 00:20
... equipment from the site; however, this is considered to pose a low risk of significant visual intrusion, in view of the small scale of equipment remaining on site Seismicity There are two types of induced ... the scope of this study, hydraulic fracturing is to be understood as the cycle of operations from the upstream acquisition of water, to chemical mixing of the fracturing fluid, injection of the ... Differences from Conventional Hydrocarbon practices None Many more shale gas wells are required for recovery of a given volume of gas than for recovery of the same volume of gas from conventional...
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Báo cáo khoa học: The phosphorylation pattern of human as1-casein is markedly different from the ruminant species potx
Ngày tải lên :
23/03/2014, 21:20
... MALDI-TOF MS analyses of the human b-casein digest, two peptides with protonated masses of 2407.85 and 2327.80 were identified These masses correspond to the expected masses of the peptide 1–18 of human ... Table The amino acid sequence of human Table Characterization of peptides from the tryptic digest of human as1-casein Peak numbers designations correspond to those of Fig The amino acid sequence ... mass of approximately 20 232 Da, which correlates well with the calculated mass of human as1-casein including two phosphate groups (20246 Da) (Fig 4) Ó FEBS 2003 Fig MALDI-TOF MS of intact human...
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Researching Design Learning: Issues and Findings from Two Decades of Research and Development pot
Ngày tải lên :
24/03/2014, 02:20
... essentially a team of experienced teachers with implicitly held views about the nature of being human; of what it means to learn; and of what is uniquely offered to those two concerns by learning ... General Certificate of Secondary Education Awarding Bodies One of the immediate outcomes of this project was the development by one of these awarding bodies of a new form of syllabus and examination ... part of the revision of 16+ examinations Prior to this point, there had been a twin system of qualifications at 16+; the General Certificate of Education (GCE), for the ‘top’ 25% of ability of...
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Báo cáo khoa học: Selection of a CXCR4 antagonist from a human heavy chain CDR3-derived phage library doc
Ngày tải lên :
28/03/2014, 22:20
... isolated from peripheral blood mononuclear cells from different species In this report, we compared the efficiency of a human HCDR3 library engineered from a nonimmunized donor with a set of conventional ... 4.1 software employing a two- state reaction model in agreement with the presence of linked reactions Analysis of amino acid sequences Ala scanning HCDR3 loops of randomly picked colonies of the ... length diversity, a set of forward and backward primers based on the nucleotide alignment of 1182 human VH sequences was used to amplify the complete diversity of the HCDR3 loops of the donor [12,30,31]...
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