developmental and regenerative potential stem cell biology and regenerative medicine

... Sources of Stem Cells for Tissue Repair Different types of stem cells include embryonic stem (ES) cells, umbilical cord blood stem cells, organ-specific somatic stem cells (e.g., neural stem cells ... pluripotent stem cell populations, such as bone marrow mesenchymal stem cells (MSCs) or adipose stem cells, for use in regenerative strategies Tissue culture evidence suggests that these stem cell ... Organ-specific multipotent stem cells are already somewhat specialized and may be easier to induce into desired cell types These cells could potentially be obtained from the patient and amplified in culture,...

Ngày tải lên: 07/07/2014, 01:20

... of different cell types, including ES cells, bone marrow stem cells, endothelial stem cells, and adipose stem cells Bone marrow stem cells in particular have been examined in clinical trials ... an ideal source of stem cells for therapeutic use For effective myocardial repair, cells must be delivered either systemically or locally, and the cells must survive, engraft, and differentiate ... cells are also excellent vehicles for cellular gene therapy (Chap 65) Figure 67-1 Strategies for transplantation of stem cells Undifferentiated or partially differentiated stem cells may be injected...

Ngày tải lên: 07/07/2014, 01:20

... pancreatic, and/ or gastrointestinal precursor cells may be candidates for cell- based therapy of diabetes Nervous System Neural cells have been differentiated from a variety of stem cell populations ... cell populations Human ES cells can be induced to generate neural stem cells, and these cells can give rise to neurons, oligodendroglia, and astrocytes These neural stem cells have been transplanted ... commencing Transdifferentiation of bone marrow and adipose stem cells into neural stem cells, and vice versa, has been reported, and clinical trials of such cells have begun for a number of neurologic...

Ngày tải lên: 07/07/2014, 01:20

... (eds): Essentials of Stem Cell Biology San Diego, Elsevier Academic Press, 2006 Mimeault M et al: Stem cells: A revolution in therapeutics-recent advances in stem cell biology and their therapeutic ... Jameson, and Ron McKay to this chapter in the 16th edition Further Readings Committee on the Biological and Biomedical Applications of Stem Cell Research et al: Stem Cells and the Future of Regenerative ... applications in regenerative medicine and cancer therapies Clin Pharmacol Ther 82(3):252, 2007 [PMID: 17671448] National Institutes of Health: Stem cell information page URL: http://stemcells.nih.gov/index.asp...

Ngày tải lên: 07/07/2014, 01:20

... Germ-line Stem Cells, 149 Amy A Kiger and Margaret T Fuller Primordial Germ Cells as Stem Cells, 189 Brigid Hogan 10 Embryonic Stem Cells, 205 Austin Smith 11 Embryonal Carcinoma Cells as Embryonic Stem ... normally demanded of stem cells in vivo and, indeed, in practice, the distinction between stem and transit amplifying cell may be difficult to make Stem cell, ” like many other terms in biology, ... Pancreatic Stem Cells, 499 Marcie R Kritzik and Nora Sarvetnick 22 Stem Cells in the Epithelium of the Small Intestine and Colon, 515 Douglas J Winton Index, 537 vii Introduction: Stem Cell Biology...

Ngày tải lên: 08/04/2014, 12:53

... culture and expand stem cells in vitro to obtain a sufficient quantity for analysis and potential therapeutic use Although the derivation of stem cells in vitro has been a major obstacle in stem cell ... cell biology, the number and types of cultured stem cells have increased progressively (Table 66-1) The cultured stem cells derived from resident stem cells are often called adult stem cells ... and pancreas, based on their specific locations, morphology, and biochemical markers Isolated Stem Cells Unequivocal identification of stem cells requires the separation and purification of cells,...

Ngày tải lên: 07/07/2014, 01:20

... Potency and source developmental stage of cultured stem cells For abbreviations of stem cells, see Table 66-1 Note that stem cells are often abbreviated with or without "cells," e.g., ES cells ... transplanting actively dividing stem cells Mouse ES cells can form teratomas when injected into immunosuppressed animals Potency and Differentiation of Stem Cells Developmental Potency The term ... cultured cells (sometimes called immortality) These features are primarily seen in ES cells, but have also been demonstrated for NS cells, MS cells, MAPCs, maGSCs (adult-derived tissue stem cells), and...

Ngày tải lên: 07/07/2014, 01:20

... MSH, McLaren A: Epigenetics of germ cells, stem cells, and early embryos Dev Cell 10:161, 2006 [PMID: 16506346] Lanza R et al (eds): Handbook of Stem Cells, vols and London, Elsevier Academic Press, ... function of stem cells and lead to a more effective means to manipulate stem cells for future therapeutic use Further Readings Cervera RP, Stojkovic M: Human embryonic stem cell derivation and nuclear ... essential molecular characteristics of stem cells It is also used to indicate common genetic programs shared among ES cells and tissue stem cells (HS and NS cells) A number of common genes, such...

Ngày tải lên: 07/07/2014, 01:20

... cancer stem and progenitor cells Stem Cells Dev 2009, 18:17-25 Ginestier C, Hur MH, Charafe-Jauffret E, Monville F, Dutcher J, et al: ALDH1 is a marker of normal and malignant human mammary stem cells ... marker for human hematopoietic stem and progenitor cells Blood 1997, 90:5002-5012 22 Wu Y, Wu PY: CD133 as a marker for cancer stem cells: progresses and concerns Stem Cells Dev 2009, 18:1127-1134 ... of ALDH and other stem cell markers The extracted xenograft cells were therefore maintained for 10-14 days in either standard RPMI medium containing fetal bovine serum (RPMI) or stem cell medium...

Ngày tải lên: 13/08/2014, 15:21

... Descriptions and definitions of each cell type in the models of stem cell differentiation Model Descriptions Definitions Pluripotent stem cell C57Bl/6 cell line A Adult neural stem cell *Neurosphere ... are neural stem cells in the adult mammalian brain Cell 1999, 97:703-716 Jordan CT, McKearn JP, Lemischka IR: Cellular and developmental properties of fetal hematopoietic stem cells Cell 1990, ... stages in the developmental hierarchy Model A [54] contains pluripotent embryonic stem cells (ESCs), MSCs in adult hematopoietic (hematopoietic stem cells (HSCs)) and neural (neural stem cells (NSCs))...

Ngày tải lên: 14/08/2014, 20:22

... organelles, and structures and functions of the bacteria covering issues from different aspects of cells including cell biology, microbiology, immunology and microscopy In terms of cell biology, cell ... accurate and concrete information to K-8 students on the correct functions of our cellular and viral system Many attempts have been made to present and simulate molecular processes in both cellular and ... supplementary material equipping students with the basics of cell biology, microbiology, immunology and microscopy within the context of Science and Technology Course SOFTWARE Sample software environments...

Ngày tải lên: 14/02/2014, 13:20

... system Biological functions of DPIV, FAP and DP8/9 The DPs have interesting roles in cell biology and in pathogenic processes DPIV and FAP have been identified both as potential cancer markers and ... recruitment of CD26 and CD45 to these rafts [80] and induces T -cell activation DPIV family in cancer and cell biology Fig Features of the effects of DP upon T -cell activation ⁄ function Cell- surface ... DP8 and DP9 immune function, and their potential as targets for inflammatory diseases As previously outlined, DP8 and DP9 are present in leucocytes and leucocyte DPIV family in cancer and cell biology...

Ngày tải lên: 06/03/2014, 09:22

... established two Ptb) ⁄ ) ES cell lines, and found that Ptb) ⁄ ) ES cells showed severe defects in cell proliferation in vivo and in vitro (Figs 3B and 4B) As Ptb) ⁄ ) ES cells exhibit a low proliferation ... blue-staining-negative cells to that of total cells Cells (3 · 105) were seeded on growth medium and counted every day for days of culture The circles indicate the values for wild-type cells and the triangles ... undifferentiated state and did not lead to precocious differentiation PTB in development and ES cells 2.5 1.5 0.5 +/+ –/– Fig Ptb) ⁄ ) ES cells have a severe defect in cell proliferation in vivo and in vivo...

Ngày tải lên: 07/03/2014, 00:20

... 2009 FEBS 1849 Biology and chemistry of b-secretase (BACE-1) C E Hunt and A J Turner BACE-1 promoter, and several studies have reported the upregulation of BACE-1 mRNA both in vitro and in vivo ... isophthalamide derivatives as the P2–P3 ligands showed good selectivity between BACE-1 and BACE-2, nanomolar potency in vitro and in cell- based studies, and a significant reduction in Ab40 levels ... 10001–10006 110 Andrau D, Dumanchin-Njock C, Ayral E, Vizzavona J, Farzan M, Boisbrun M, Fulcrand P, Hernandez JF, Martinez J, Lefranc-Jullien S et al (2003) BACE1- and BACE2-expressing human cells: characterization...

Ngày tải lên: 07/03/2014, 00:20

... pressure on the cell without causing the cell to burst 31 Plants have cell walls made of cellulose During cell division plant cells build dividing walls between the two new cells called the cell plate ... known about cells has increased greatly Cell Theory • Cells are the fundamental unit of life - nothing less than a cell is alive • All organisms are constructed of and by cells • All cells arise ... processes are carried out by cells • Groups of cells can be organized and function as multicellular organisms • Cells of multicellular organisms can become specialized in form and function to carry...

Ngày tải lên: 13/03/2014, 17:52

... all students Correlation to Cell Biology and Cancer should develop understanding of the cell • Cells store and use information to guide their functions Activities and • Cell functions are regulated ... Activities 1, 4, and • natural and human-induced hazards Activities 1, 4, and 23 Ä Cell Biology and Cancer • science and technology in local, national, and global challenges Activity Standard G: As ... growth-stimulating pathways In normal cells, inhibitory messages flow to a cell s nucleus 11 Cell Biology and Cancer A second cellular back-up system prompts a cell to commit suicide (undergo apoptosis)...

Ngày tải lên: 15/03/2014, 00:20

... PROJECT OVERVIEW AND DEFINITIONS ADULT STEM CELLS 19 EMBRYONIC STEM CELLS 31 OPPORTUNITIES FOR AND BARRIERS TO PROGRESS IN STEM CELL RESEARCH FOR REGENERATIVE MEDICINE 41 FINDINGS AND RECOMMENDATIONS ... manipulation of the stem cells and the development of a very large bank of embryonic stem cell lines In conjunction with research on stem cell biology and the development of stem cell therapies, ... embryonic and adult human stem cells will be required to most efficiently advance the scientific and therapeutic potential of regenerative medicine Research on both adult and embryonic human stem cells...

Ngày tải lên: 15/03/2014, 09:20

... the Rossmann-fold topology and a substrate-binding domain Evolution has ‘modeled’ the structure of these AOD enzymes to fit different amine substrates and cellular landscapes LSD1 was the subject ... diagrams, the AOD is colored blue and the FAD molecule is in a yellow ball -and- stick representation The N- and C-terminus of each structure are labeled as ‘N’ and ‘C’, respectively In each structure, ... [35] and preceded by a segment of 200 residues that includes a SWIRM domain and a putative unstructured region (Figs 3A and 4) The Tower domain protrudes from the globular AOD structure and provides...

Ngày tải lên: 16/03/2014, 02:20

... and Stem Cell and Other Therapies disease initiation method, with a single and defined myelin peptide allows for the observation and measurement of changing T cell specificities over time (Vanderlugt ... helper cells The cytotoxic CD8+ T cells then affect beta -cell damage by releasing perforin and granzyme, and by Fas-mediated apoptosis In this way, macrophages, CD4+ T cells, and CD8+ T cells ... islet beta-cells in the pancreas (Toyoda and Formby 1998) Understanding insights of the mechanism of immune-mediated islet cell destruction and the interaction between the immune system and pancreatic...

Ngày tải lên: 16/03/2014, 21:20