development of targeted treatments for nf1 tumors

... Determination of the level of RNA products of the H19 gene The PCR reactions were carried out in 25 μl volumes in the presence of ng/μl of each of the forward and the reverse primers using 0.05 units/μl of ... compared to Luc-H19/PEI treatments (P < 0.05) Discussion The present work shows the use of the regulatory sequences of the H19 gene for the development of DNAbased therapy for human ovarian cancer ... polymerase chain reaction (PCR) was carried out for 30 cycles (98°C for 15 sec, 58°C for 30 sec, and 72°C for 40 sec and finally 72°C for min) The integrity of the cDNA was assayed by RTPCR analysis...

Ngày tải lên: 18/06/2014, 15:20

... of 94°C for 30 sec, 59°C for 45 sec and 72°C for 60 sec, and finally 72°C for The PCR reactions were carried out in 25 μl volumes in the presence of ng/μl of each of the forward and the reverse ... successive slides of TCC tissue for H19 and IGF2-P4 transcripts The intensity of hybridization signal was indicated as (0) for no staining, (+1) for weak, (+2) for moderate and (+3) for strong signals ... carefully Samples of the tumors were fixed in 4% buffered formaldehyde and processed for histological examination for evidence of necrosis and persistent tumor Computerized measurements of tumor surface...

Ngày tải lên: 18/06/2014, 16:20

... International Journal of Energy and Environment (IJEE), Volume 4, Issue 3, 2013, pp.415-426 Output of the process Step input r(t) Performance of G1(z) -0.2 Performance of G2(z) Performance of G3(z) -0.6 ... responses considering three points’ performance in group Output of the process Step input r(t) Performance of G1(z) -0.4 Performance of G2(z) Performance of G3(z) -0.6 -0.8 r(t),y (t),y (t),y ... controller for temperature control inside a room within a desired band of temperatures for comfort The details of the geometry of the room and the HVAC system based on displacement ventilation for cooling...

Ngày tải lên: 05/09/2013, 16:11

... for the cattle, and herb for the service of man: that he may bring forth food out of the earth 'And wine that maketh glad the heart of man The Development of the Feeling for Nature in the Middle ... suggestive myth of Baldur, god of light and spring, killed by blind Hửdur, was the expression of general grief at the passing of beauty The Edda has a touching picture of the sorrow of Nature, of her ... collective picture of heaven and earth, of cloud movement, of the mute life of plants that side of Nature which had almost escaped the eye of antiquity occupied the Northerner most of all 'The Edda...

Ngày tải lên: 21/02/2014, 20:20

... (0.01%) coated wells of a 96-well plastic plate for h at 37 °C Corresponding dilutions of KLH alone, membrane fraction of nontransformed yeast, or membrane fraction of yeast transformed with the pJH2-SSTR2 ... presence of plasmids in transformed cells was verified by PCR on nucleic acid extracts Induction of I7 expression was performed as reported for the SSTR2 induction [24] with the exception of the ... commonly used for pharmacological studies of recombinant ORs This probably arises from the poor affinity of Ga15 for yeast Gbc, as such a lack of affinity has already been reported for Gpa1 ⁄ Ga15,16...

Ngày tải lên: 07/03/2014, 16:20

... semantic labels taken from the DDC The development WN-PDDC as generic resource (see figure 2) Before starting the development, the set of field labels relevant for the application must be identified ... always be profitably applied It also provides a criterion for building lexical resources for specific domains It can be applied in a semiautomatic way It has the advantage of using the information ... quality and quantity of terms and development time of the whole lexicon One of the issues that we are currently investigating is that of choosing the correct set of field labels from DDC: DDC is...

Ngày tải lên: 08/03/2014, 21:20

... stage of the research As a result, the sample of students was selected for the next stage of the research to create the model for the development of students’ ESP competence Development of students’ ... means of development of students’ ESP competence The scientific novelty of the research also includes the created model for the development of tourism students’ ESP competence The basis of the ... have to provide the unity of the studies and practice; the ESP course has to further the development of students’ knowledge of professional lexis and the development of their listening and speaking...

Ngày tải lên: 29/03/2014, 23:20

... 3A–C) Most of the cells in the lymphoid system of scid mice are NK cells, except for a few immature B or T cells due to 'leakiness' of the scid system, Page of 13 (page number not for citation ... demonstrate which of the acquired mutations were important for adaptation to mice Figure of potential therapeutics MARV model to assess the efficacy Use of the 'scid-adapted' for MARV Use of the 'scid-adapted' ... number of circulating white blood cells (WBC), percentage of lymphocytes, and absolute numbers of lymphocytes within the blood of the 'scid-adapted' MARVinfected mice, the very low number of circulating...

Ngày tải lên: 18/06/2014, 18:20

... 3A–C) Most of the cells in the lymphoid system of scid mice are NK cells, except for a few immature B or T cells due to 'leakiness' of the scid system, Page of 13 (page number not for citation ... demonstrate which of the acquired mutations were important for adaptation to mice Figure of potential therapeutics MARV model to assess the efficacy Use of the 'scid-adapted' for MARV Use of the 'scid-adapted' ... number of circulating white blood cells (WBC), percentage of lymphocytes, and absolute numbers of lymphocytes within the blood of the 'scid-adapted' MARVinfected mice, the very low number of circulating...

Ngày tải lên: 20/06/2014, 01:20

Ngày tải lên: 21/06/2014, 06:20

... understanding of key course knowledge elements About 2/3 of participants had applied knowledge and skills learned in the course in their professional work in plantation forestry Table Summary of follow-up ... understanding of key course knowledge elements Most of the participants had applied one or more areas of knowledge and skills learned in the course in their professional work in plantation forestry ... could be located were interviewed by telephone by Ms Duong Thanh Hoa of FSIV A total of 13 of the 25 attendees were contacted for this assessment Summary results are shown in Table The summary...

Ngày tải lên: 21/06/2014, 06:20

... Organisation Forest Service Department of Thua Thien Hue Forest Service Department of Thua Thien Hue Management Board of Sandy Forest Management Board of Sandy Forest Management Board of JBIC Project ... entirely of participants with at least some professional training in an aspect of forestry If farmers had been the audience it would have been difficult for them to understand a lot of the information ... edge of root collar (role of root collar in occlusion of branches); o Avoid all damage to bark; undercutting large branches before pruning can eliminate bark tearing • Selecting branches for form...

Ngày tải lên: 21/06/2014, 06:20

Ngày tải lên: 21/06/2014, 06:20

... deployment of selected clones with outstanding stem form Significant improvement in stem form is also achievable through use of improved seedling stock of A auriculiformis and A mangium Little information ... Booth of CSIRO Forestry and Forest Products which enables display of areas in Vietnam satisfying any set of climatic requirements such as those shown in Table This software has been used by the Forest ... clone in the foreground (dbh 18 cm), two-tree plot of a very poor clone behind the observer 5.3 Development of acacia hybrid clones The development of highly productive selected clones of the interspecific...

Ngày tải lên: 21/06/2014, 06:20

... submitted Implementing Report submitted Development of skilled human resources Development of skilled human resources Development of skilled human resources Development of skilled human resources Minimum ... DECLARATION COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT PROGRAM CARD Project Title: - Development of clam culture for improvement and diversification of livelihoods of the poor coastal communities ... performance of clam in the different treatments This analysis has been done to understand average and specific growth performance under different treatments It may be noted that, in the case of...

Ngày tải lên: 21/06/2014, 06:20

... guidelines for Acacia plantations managed for sawlog production in Vietnam These guidelines are based on two reviews carried out for the CARD Project 032/05 “Sustainable and profitable development of ... absence of more specific information, planting holes dug about one month before planting where two-thirds of the hole is filled with a mixture of top soil and fertiliser is recommended The size of ... may be necessary for a longer period of time Pruning# A first-lift pruning should be undertaken at the time of canopy closure and before crown lift has commenced Trees selected for pruning will...

Ngày tải lên: 21/06/2014, 06:20

... m3/ha for A auriculiformis and 40 - 50 m3/ha for A mangium for rotations of - years This equates to MAI’s of around 4-5 m3/ha for A auriculiformis and 5-7 m3/ha for A mangium Establishment cost for ... achieve a stumpage value of $US 2100 at the end of year This represents a net present value of $512 for the net return on the investment and an internal rate of return of 24% Many farmers now ... and forest extension staff Using formulas for discounted cash flow, the model calculates net present value (NPV) assuming a real (i.e net of inflation) discount rate of 10%, and internal rate of...

Ngày tải lên: 21/06/2014, 06:20

... submitted Implementing Report submitted Development of skilled human resources Development of skilled human resources Development of skilled human resources Development of skilled human resources with ... DECLARATION COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT PROGRAM CARD Project Title: - Development of clam culture for improvement and diversification of livelihoods of the poor coastal communities ... be recommended for clam culture in the intertidal area at stocking size of 1cm height for period of months Table Economical evaluation of clam rearing at stocking size of 1.7cm Treatments T1 T2...

Ngày tải lên: 21/06/2014, 06:20

... poor form • • Remove trees that are of poor form in the first 4.5 m of the stem The main reason for this will be large forks where the stem has split into at least two leaders Trees of poor form ... Dong Hoi thinning trial Collaboration for Agriculture and Rural Development (CARD) Program Sustainable and profitable development of acacia plantations for sawlog production in Vietnam Objective ... The trial has for replicates, with plots of four thinning treatments in each replicate It should provide valuable data on growth response to thinning of the acacia hybrid Total area of the trial,...

Ngày tải lên: 21/06/2014, 06:20

... Statistical for Graph-Pad Prism-4 Demo software Data are presented as mean ± standard deviation Result - Growth rate of larvae in different frequency of exchange of water Table 5.Mean ± SD of growth of ... DECLARATION COLLABORATION FOR AGRICULTURE AND RURAL DEVELOPMENT PROGRAM CARD Project Title: - Development of clam culture for improvement and diversification of livelihoods of the poor coastal communities ... Department of Nghi Loc District, Nghe An Province Technical of Fisheries Department of Dien Chau District, Nghe An Province Technical of Extension Centre of Ha Tinh Province Technical of Extension...

Ngày tải lên: 21/06/2014, 06:20