development and application of genomic models for large crop plant genomes

... division and DSB formation, 15 random images were acquired for lung sections co-stained with Ki-67 and cH2AX The number of nuclear Ki-67-positive cells in each 0.1 mm2 lung area, and the proportion of ... inset) at and dpi Scale bar 20 lm phosphorylated cH2AX by western blot (Fig 4a) Relative intensities of HA and cH2AX bands were quantified and normalized to b-actin, and the levels of HA and cH2AX ... proteins involved in NHEJ and HR a Western analysis of the NHEJ proteins, Ku86 and Ku70 b, c Quantification of the levels of Ku86 (b) and Ku70 (c) Levels of Ku86 trend upward and are statistically...

Ngày tải lên: 09/09/2015, 08:16

... database containing tens of millions of expression profiles Information and microarray expression data for genes involved in mitosis and meiosis, gamete formation and germ line development across species ... source of gene expression data, microarray data is used in this study for gene selection Microarray measures the expression profiles of thousands of genes at the same time and have been explored for ... 5.2 The development of antibody information database 142 5.2.1 The objective of the AAIR development 142 5.2.2 The collection of related information 143 5.2.3 The construction of AAIR...

Ngày tải lên: 12/09/2015, 08:20

... committee members Professor Fred Wong and Professor Marie Clement for their invaluable comments and suggestions for my thesis, and to my collaborators, Dr Damien Thévenin and Professor Donald Engelman ... Acknowledgements: First and foremost, I would like to express my sincere gratitude to my supervisors Professor Bevin Engelward and Professor Vincent Chow, for offering me the opportunity to be a part of their ... chemicals and reagents .42 Cell cultures .43 List of antibodies for western blot and immunofluorescence .44 List of antibodies for flow cytometry 45 Source of influenza...

Ngày tải lên: 30/09/2015, 06:20

Ngày tải lên: 14/11/2014, 12:55

... required upon hand-off for assay validation SJ, AW, SWA and KA performed the in vitro assays on monkeys treated with Ab-01 and control Ig and analyzed the data, and KA and SA performed the experiments ... phase of the project AGB performed all in vivo procedures on monkeys and contributed to the writing of the manuscript MFS was responsible for interface with the clinical team and for securing and ... (Sequence Detector Software v2.2.2) using universal thermal cycling conditions of 50°C for minutes, 95°C for 10 minutes, followed by 40 cycles of 95°C for 15 seconds and 60°C for minute Data output...

Ngày tải lên: 18/06/2014, 16:20

... and probes for SCO4410, SCO5639, SCO3202 and SCO4157 were designed using Primer software and used for qPCR as described previously [7] The sequences for the forward and reverse primers and dual ... Bioconductor: open software development for computational biology and bioinformatics Genome Biol 2004, 5:R80 R [http://www.R-project.org] R Development Core Team: R: a Language and Environment for Statistical ... dispersed growth of the mycelium and provide sufficient biomass for the ChIP protocol; this was to maximize formaldehyde penetration and to determine the genomic distribution of HspR under non-stressed...

Ngày tải lên: 14/08/2014, 21:20

... in units of two or three, and the combined action of salivary and pancreatic amylase leads to the production of large amounts of maltose and maltotriose, and relatively small amounts of limit ... the world for the centuries, and many of them have been studied and recommended as alternative treatments for diabetes [7] Babu and coworkers have created a database of 389 medicinal plants that ... DEVELOPMENT AND APPLICATION OF A HIGHTHROUGHPUT SCREENING ASSAY FOR DISCOVERY OF STARCH HYDROLASE INHIBITORS LIU TING TING (BSc, MSc Wageningen University) A THESIS SUBMITTED FOR THE DEGREE OF...

Ngày tải lên: 10/09/2015, 09:01

... 67 Table 6.1 Performances of best base models and best ensemble models for SJS/TEN study 84 Table 6.2 Performances of best base models and best ensemble models for TdP study ... 6.3 Performances of best base models and best ensemble models for serious psychiatric ADR study 86 Table 7.1 Performance profile of SVM models on testing and validation set for AM ... performances of most of the models are promising and some of the models achieve sensitivity and specificity values higher than 90% This demonstrates the huge potential of the application of the...

Ngày tải lên: 10/09/2015, 09:02

... Theories of droplet-based LPME 1.1.2.1.2 Developments and applications of droplet-based LPME 1.1.2.2 Membrane-based LPME 1.1.2.2.1 Theories of membrane-based LPME 13 13 1.1.2.2.2 Developments and applications ... effective combination of Lin and Dasgupa’s [37] and Jeannot and Cantwell’s [34] earlier works Enrichment factors of between 260- to 1600-fold were achieved within 10 of extraction of trace nitroaromatic ... model for carrier-mediated extraction Modified from ref [55] 1.1.2.2.2 Developments and applications of membrane-based LPME For membrane-based LPME, there are a few considerations in term of the...

Ngày tải lên: 12/09/2015, 08:20

... number of S and hence of A deteriorates when the mesh is refined, with ∆t being fixed by the maximum frequency of interest For large κ(A), nCG ∝ κ(A) [9], [42] and hence the number of iterations for ... equivalence of FDTD and a particular case of FETD method a stable hybrid 3-D FETD-FDTD method was proposed The focus of this thesis is in the development and subsequent applications of efficient ... for better field representation and use of a coarser mesh Computation of the modal reflection coefficient, input impedance and radiation pattern of real world antennas and the results obtained for...

Ngày tải lên: 14/09/2015, 11:39

... Instrumentation and Modes Since the introduction of commercial CE instrumentation from late 1988, the speed of development and application of this technique was enhanced Requirement of only simple ... Electropherogram of Environmental Water by FASS-CE-CCD……… ….………… ………………………… ……… 178 XXIV List of Symbols List of Symbols α normalized length of a plug σ total spatial variance of the concentration profile for ... instrumentation and modes, existing techniques to improve CE performance, and applications of CE as well A novel potential gradient detector (PGD) was designed and coupled with a portable CE system for separation...

Ngày tải lên: 14/09/2015, 11:40

... DEVELOPMENT AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... large- scale analysis of proteins, i.e proteomics (Pandey and Mann, 2000; Tyers and Mann, 2003) Therefore, the advancement of proteomics relies largely on the development of state -of- the-art proteomics ... Tandem Affinity Purification TOF Time of Flight TOF/TOF Time -of- Flight/Time -of- Flight UV Ultraviolet Y2H Yeast Two-Hybrid xvi Chapter Introduction The complete sequence of the human genome (Lander...

Ngày tải lên: 16/09/2015, 08:30

Ngày tải lên: 21/02/2014, 09:20

... in both dispersed and heterogeneous forms (supported) tsnl Advantanges of this Approach•The light absorption and energy levels of the semiconductor valence and conduction bands can be adjusted ... Treatment of large volumes of highly diluted toxins 3) Cost of treatment tsnl Possible Treatment ApproachesStep 1: Excavation, Soil Washing Conventional Treatment Options: 1)Filtration and/ or Adsorption ... characterization of the photocatalyst microstructure tsnl Photocatalysts Material Requirements 1) Efficient conversion of sunlight to electron-hole pairs 2) Surface trapping of electrons and holes before...

Ngày tải lên: 14/03/2014, 20:20

... 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of 2MF9AA 61 Synthesis and mass spectrum of 3MF9AA ... great promise as a field instrument for the rapid monitoring of PAH pollution xi The Development and Application of an Antibody-based Biosensor for the Detection of Petroleum-derived Compounds INTRODUCTION ... LIST OF TABLES Table Page Comparison of traditional and immunoassay techniques 11 Illustration of structural similarities of derivatized haptens and target analytes 43 NMR assignments for...

Ngày tải lên: 13/05/2014, 14:01

... cinegomorhc eht dna ,nim 51 rof detabucni erew setalp ehT dedda saw )ASU ,LPK ;tanoflusnilozihtneb-lyhte-3-id-oniza-'2.2( STBA etartsbus eht fo emulov lacitnedi na ,h rof Co73 ta noitabucni retfA ... rof yllacipocsorcim denimaxe saw llew hcaE syad rof 2OC %5 rednu ,Co73 ta detabucni erew setalp ehT llew/sllec 000,02 fo tnuoc llec lanif a ot erutxim eht ot dedda erew sllec 401FT eht ,h rof ... emulov lauqe na htiw dexim erew ares eht fo snoitulid dlof-owt laireS sllec 401FT gnisu setalporcim llew-69 a ni demrofrep saw nim 03 rof Co65 ta ares detavitcani eht htiw tset NS ehT evitisop...

Ngày tải lên: 07/08/2014, 18:21

... efficiency of the development of large amounts of microsatellite markers for coniferous species 3.2 Characterization and polymorphisms of microsatellite markers PCR primer pairs were able to design for ... two measures of variation (the number of alleles (NA) and polymorphism information content (PIC)) on one hand, and four measures of length [the number of repeats (NOR), the number of nucleotides ... average of 0.620, respectively (Tab IV) We then examined the correlations between two measures of polymorphic levels (NA and PIC) on one hand, and two measures of the length of repeat unit (NOR and...

Ngày tải lên: 08/08/2014, 01:22

... [2-7] and in children [8], and the development of multiple conditions including osteoarthritis of the foot [9,10] and knee [11], low back pain [12,13], foot ulcerations and amputations [14], and ... heel:forefoot profile will plantarflex a shorter foot more than a longer foot Page of 12 (page number not for citation purposes) Journal of Foot and Ankle Research 2009, 2:10 Last shape The last of ... and 81% of older people [23,25,27], 88% of females aged 20 to 60 years of age [24], and 80% of patients attending a general diabetic clinic [53] However, the reliability of previous methods of...

Ngày tải lên: 10/08/2014, 21:23

... SCAR reaction was performed with an initial denaturation step at 95°C for five minutes, followed by 35 cycles of 94°C for two minutes, 60°C for one minute, 72°C for one minute and a 10-minute final ... 0.25 kb for CAPS markers Ca-Afl0.30 and Ca-Afl-0.25, and 0.35 kb and 0.20 kb for CAPS markers Ca-Drd-0.35 and Ca-Drd-0.20 that could be easily visualized The species-specific patterns of CAPS ... black, and ddTTP red from both B nivea var nivea and B nivea var tenacissima in the ratios of 9:1, 8:2, 7:3, 6:4, 5:5, 4:6, 3:7, 2:8 and 1:9 respectively Results and Discussion RAPD markers for...

Ngày tải lên: 13/08/2014, 14:20

... times of ULOQ and diluted with blank plasma to get 1/5 and 1/10 concentrations of the spiked sample or as per requirement Calibration standards and six aliquots each of diluted samples (1/5 and ... Preparation of standard and sample solutions Standard stock solutions of naftopidil (1 mg/mL) and the IS (1 mg/mL) were separately prepared in 10 mL volumetric flasks with methanol Working solutions for ... 393.5/205.4 260.3/116.2 and and and and 10 (IS) 40 (IS) 25 (IS) (IS) Method validation 3.1 Specificity To verify the absence of interfering substances around the retention time of analytes, 20 blank...

Ngày tải lên: 02/09/2015, 13:48