determination of fy y from fx x

... Bisphenol A (2,3-dihydroxypropyl) glycidyl ether Bisphenol A (3-chloro-2-hydroxypropyl)(2,3-dihydroxypropyl) ether Bisphenol A (3-chloro-2-hydroxypropyl)glycidyl ether Bisphenol F diglycidyl ether Bisphenol ... FCM FP 1-hydroxycyclohexyl-phenylketone Accelerated solvent extraction Bisphenol A diglycidyl ether Bisphenol A bis(2,3-dihydroxypropyl)ether Bisphenol A bis (3-chloro-2-hydroxypropyl) ether ... widely known photoinitiators used for food applications include benzophenone (BP), 1-hydroxycyclohexylphenylketone (1-HCPK), isopropyl-9H-thioxanthen-9-one (ITX), benzyldimethyl ketal, 2-ethylhexyl-4-dimethyl-amino...

Ngày tải lên: 14/09/2015, 08:39

... acidity and the product extracted in chloroform After methylation with diazomethane it was analysed by GLCMS The absolute configuration of 2-hydroxy and 3-hydroxy fatty acids was determined by GLC ... secondary fatty acid on the proximal GlcN I was inferred by MALDI-TOF of the de-O-acylated product with ammonium hydroxide at 20 °C for 18 h This mild procedure is able to split the acyl and acyloxyacyl ... were assigned to tetracyl species with two acyloxyacyl amides in which the secondary fatty acids are C12:0 (2-OH) or C12:0 Thus, the hydrolysis of only these two primary fatty acids linked as esters...

Ngày tải lên: 24/03/2014, 00:21

... was subjected to compositional analysis and revealed the presence of 3-hydroxymyristic acid together with minor amounts of palmitic, 3-hydroxypalmitic, 2-hydroxy-palmitic and stearic acids Monosaccharide ... labile Kdo is only partly destroyed and a major peak (oxonium ion: m/z 375) is detected at 26.620 The fatty acid methyl esters were recovered by extraction with n-hexane and analysed by GC-MS The ... ionization energy of 70 eV and a ionizing current of 0.2 mA Glycosyl-linkage analysis of LPS, was performed according to the procedure of Sandford [10] The permethylated lipopolysaccharide was...

Ngày tải lên: 24/03/2014, 04:21

... hospital of the Medical Faculty of Van Yuzuncu Yil University (Van, Turkey) Identification methods: Identification of species was made on the basis of the requirement of CO2 for growth, production of ... susceptibility tests Antimicrobial susceptibility testing: Minimum inhibitory concentration (MIC) of doxycycline (DOX), rifampin (RIF), streptomycin (STR), tigecycline (TIG) and trimethoprim-sulfamethoxazole ... simultaneously exist in the same countries in many parts of the world [32] Furthermore experimental studies suggested that the development of mycobacterial resistance to RIF may lead to development of...

Ngày tải lên: 25/10/2012, 11:10

... analysis was conducted to unsure specificity of the primers and probe Real-time PCR was performed with the iCycler IQ ystem (Biorad, USA) µl of extracted DNA was analyzed with the PCR mixture ... can be explained by lower efficiency of C pneumoniae DNA extraction Sufficient recovery of final PCR product has been also seen when protein A from Staph aureus has been used for isolation of C ... diagnostically relevant levels of IgA Simultaneous detection of increased titers of IgG and IgA was documented only in patients Surprisingly, when DNA specimens extracted from 1.0 ml of serum aliquots...

Ngày tải lên: 26/10/2012, 08:57

... Syncope, the transient loss of consciousness with spontaneous recovery occurs as consequence of a period of cerebral hypoxia A number of conditions may disturb cerebral oxygenation, ranging from ... arrhythmias to periods of autonomic nervous system decompensation resulting in systemic hypotension and bradycardia In some individuals, global cerebral hypoxia may result not only in loss of ... Autonomically mediated forms of reflex syncope (such as neurocardiogenic or vasovagal syncope) may produce sudden episodes of profound hypotension and bradycardia resulting in loss of consciousness and,...

Ngày tải lên: 26/10/2012, 09:53

... of these enzymes synthesize 4-keto-6-deoxy-GDP-D-mannose, a key intermediate in the biosynthesis of many deoxyhexoses as GDP-D-rhamnose [31,32], which is a moiety of A201A Upstream of ata12 we ... sequencing of the ata cluster could provide an insight into the biosynthetic pathway of the other moieties of A201A and provide probes which may be useful to identify genes of the hygromycin A biosynthetic ... identification and quantification of puromycin were achieved by a Pac enzymatic assay [21] Preparation of 3¢-amino-3¢-deoxyadenosine 3¢-amino-3¢-deoxyadenosine was obtained from Helminthosporium sp ATCC20154...

Ngày tải lên: 21/02/2014, 01:21

... antibody [18] Conventional pepscan analysis however, does not allow easy analysis of the contribution of the carbohydrate portion To assess the involvement of carbohydrates in antibody recognition of ... obtained from PerSeptive Biosystems (Wiesbaden, Germany), acetonitrile from Alfa (Karlsruhe, Germany), triisopropylsilane and D2O from Sigma Aldrich (Steinheim, Germany), all other chemicals of analytical ... TOCSY spectrum of the PDTRP/SM3 complex are shown The peaks of Arg4 and Pro5 are so low in intensity that they not appear at the intensity cutoff shown Signals of Pro1, Asp2 and Thr3 are clearly...

Ngày tải lên: 21/02/2014, 15:20

... glutamine hydrolysis by CTP synthase The complete hydrolysis of glutamine necessary for calculating the molar enthalpy DH, and subsequently the rate of ể FEBS 2002 Glutaminase activity of CTP synthase ... the same type of allosteric activation of glutamine hydrolysis takes place by the phosphorylation of UTP on CTP synthase, only that for CTP synthase this allosteric effect exerted by the amino ... Another enzyme, that also catalyzes amino transfer from glutamine, is carbamoyl phosphate synthase (CPS) which is the rst enzyme of the de novo pyrimidine biosynthesis For CPS, glutamine hydrolysis...

Ngày tải lên: 21/02/2014, 15:20

... Peptide Synthesizer using Fmoc chemistry with pentrafluorophenyl amino acid active esters and a polyethylene glycol polystyrene support with a 5-(4¢-Fmoc-aminomethyl-3¢-5¢-dimethoxyphenoxy)valeric ... limited proteolysis of the inhibitory pro-sequence segment and may be either autocatalytic or involve accessory molecules The length of propeptide may vary and range from short polypeptide segments ... calculated from Vmax/[E], where [E] represents enzyme concentration The identity of hydrolyzed peptide fragments was determined by NH2terminal sequence and mass spectral analysis Kinetic studies of C5a...

Ngày tải lên: 08/03/2014, 16:20

... resonance study of the role of nitric oxide and catalase in the activation of guanylate cyclase by sodium azide and hydroxylamine Modulation of enzyme responses by heme proteins and their nitrosyl derivatives ... ferric when hydrogen peroxide binds Possibly hydrogen peroxide oxidizes the ferric iron center of chlorite dismutase, as it does in metmyoglobin [10] In the case of myoglobin, as with many heme proteins, ... relatively weak as in guanylate cyclase Perhaps, as in guanylate cyclase, cleavage of the Fe–His bond is part of the catalytic mechanism of chlorite dismutase Fig Schematic view of the formation of...

Ngày tải lên: 08/03/2014, 16:20

... biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation Also O-glycosidic glycans were recovered from wolffish kininogen The permethylated O-glycan pool was analysed by MALDI-TOF ... samples of the kininogens were subjected to N-glycan liberation by N-glycosidase F The released glycans were isolated by gel filtration and N-glycan pools were analysed by MALDI-TOF MS The spectrum of ... (saponification) of the N-glycans yielded glycans devoid of additional acetyl groups, an indication that these were O-acetyl substituents [15] The MALDI-TOF spectrum of the de-O-acetylated glycans reveals...

Ngày tải lên: 08/03/2014, 23:20

... dimensions of approximately 50 · 40 · 25 A, including three disulfide bonds (Cys20–Cys67, Cys50– Cys57 and Cys158–Cys187) and five cis peptide bonds Two of the cis peptide bonds of PPL2 (Gly147–Lys148 ... identity with plant chitinases belonging to glycosyl hydrolase family 18, has not been shown to have any chitinase activity [41] The lack of chitinase activity of concanavalin B can be explained by ... platycephala lectin (PPL2), is homologous to endochitinases of the glycosyl hydrolase family 18 that exhibit rabbit erythrocyte-agglutinating, N-acetylglucosamine-binding and chitin-hydrolyzing...

Ngày tải lên: 16/03/2014, 13:20

... described by Davies et al [22] The expression of wild-type OpaJ129 was determined by Western blotting using monoclonal antibody 15-1-P5.5 [18] The expression of wild-type OpaB128 was determined by Western ... OpaJ129 Fig Binding of His-tagged N-domains of CEACAM1 and by OpaJ129- and OpaB128-expressing E coli cells The binding of the N-terminal fragments of CEACAM1 and CEACAM8 by MS11OpaB-expressing E coli ... significant contamination from different Opa proteins expressed from other loci However, for detailed structure–function analysis pure protein is needed and by cytoplasmic expression of OpaB128 and OpaJ129...

Ngày tải lên: 17/03/2014, 10:20

... determine the photon flux The main advantages of this method are high sensitivity, accuracy and the experimental procedure is rather simple and feasible By this way, the photon intensity can be determined ... energy The average activity of the activation foils served as photon flux to which the foils were exposed The relation between the average photon flux, φ, and the number of detected gamma rays, ... computer based multichannel analyzer system The energy resolution of the system is 1.8 keV at 1.332 of 60Co standard source The gamma spectra were measured and analyzed by the program S100 (Canberra)...

Ngày tải lên: 22/03/2014, 11:20

... chromatography (EtOAc/ hexanes, : 1) gave the per-acetylated glycosyl glycerol N-Acetyl glycosyl glycerol De-O-acetylation was performed on per-acetylated glycosyl glycerol by treatment with 0.01 ... and 430, from N-acyl hexosamine are indicative that the hexosamine was acylated by two major fatty acids, C17:0 (m/z 414) and hydroxy (presumably 3-hydroxy) C17:0 (m/z 430) The ratio of the C17:0 ... analyzed by HPAEC-PAD For the carbohydrate linkage analysis, the Hakomori methylation analysis [17] was carried out The glycolipid or N-acetyl glycosyl glycerol was per-O-methylated with methyl...

Ngày tải lên: 23/03/2014, 13:20

... injection of F9 cells, in which HSF2 is highly expressed, under the skin of syngenic mice We verified that extracts produced from tumor cells displayed an HSE-binding activity similar to that of extracts ... Purification to homogeneity of HSF2 from F9 tumor extracts Elution fractions from the first and second cycle of HSE-affinity column (as well as HSF2 synthesized in reticulocyte lysates) were run on SDS/PAGE ... trimer separated from the two fixed trimers by a TA dinucleotide This result was confirmed by the next rounds of selection (Fig 6) The SELEX assay was stopped after six cycles of selection/amplification...

Ngày tải lên: 24/03/2014, 00:21

... glycolysis from glucose and gluconeogenesis from the added glycerol The rate of carbohydrate synthesis from glycerol was  60% of the rate of glycolysis from 40 mM glucose and exceeded that of ... as glycolytic products It can be envisaged that the operation of a redox couple between Gro-3-P and pyruvate, generated during glycolysis from glucose, facilitates the entry of some dihydroxyacetone ... glycolysis from 10 mM glucose by 20% and, under these conditions, about 17% of the glycerol carbon was diverted to glycolytic products Glycerol appears to inhibit glycolysis from glucose by two...

Ngày tải lên: 24/03/2014, 03:21

... composition of D hottai GSLs, we noticed that adults have no hydroxy fatty acid, plerocercoids have predominantly trihydroxy sphingoids, and hydroxy fatty acids are always bound with trihydroxy sphingoids ... GalSEGLx Sphingoid b Dihydroxy c Trihydroxy t18:0-C14:0 t18:0-C16:0 t18:0-C18:0 t18:0-C26h:0 t18:0-C18h:0 t20:0-C18h:0 d Fatty acid e Hydroxy fatty acid f Values are expressed as percentages of the ... were analysed as trimethylsilyl derivatives by GC/MS For methylation analysis, partially methylated alditol acetates were prepared from purified GSLs and analysed by GC/MS Detailed analytical procedures...

Ngày tải lên: 24/03/2014, 03:21

... due to the cofactor was significantly lower than that of the wild-type enzyme and, correspondingly, the amount of PLP released by NaOH treatment was only 10% of that expected for a fully saturated ... (1976) Band-shape analysis and resolution of electronic spectra of pyridoxal phosphate and others 3-hydroxypyridine-4-aldehydes Biochim Biophys Acta 421, 181–194 O’Leary, M.H & Malik, J.M (1972) ... A (1999) Lysine-69 plays a key role in catalysis by ornithine decarboxylase through acceleration of the Schiff base formation, decarboxylation, and product release steps Biochemistry 38, 11814–11826...

Ngày tải lên: 31/03/2014, 08:20