determination of antibody glycosylation by mass spectrometry

... corresponding with Simultaneous Determination of Various Macrolides by Liquid Chromatography /Mass Spectrometry 105 F i g T ot a l ion ch r om a t ogr a p h y (TI C, A) of m a cr olid e a n d t ia ... the simultaneous determination of macrolides The fragmentation patterns provide the confirmatory information of macrolides The relevance of these studies for the determination of macrolide in ... curves have been constructed by plotting area against the standard concentrations of macrolides in the range of 0.001 ㎍/㎖ ~ ㎍/㎖ Limit of detection (LOD) and limit of quantitation (LOQ) were based...

Ngày tải lên: 07/08/2014, 15:20

... polymorphism of apolipoprotein C1 M S Wroblewski et al also suggest approaches that might be used to determine the role of N-terminal truncation of ApoC1 Results Profile analysis The MALDI-TOF mass spectrometer ... shows an overview of the plasma protein profile Briefly, peak identification was accomplished by comparison of m ⁄ z values with those of known plasma proteins, Edman degradation of the entire sample ... 4708 expected N-terminal residue of each component, and ⁄ or C-terminal degradation by carboxypeptidase with removal of mass appropriate for the expected residues of each protein An additional approach...

Ngày tải lên: 19/02/2014, 05:20

... In Fig 2A three MALDI-TOF mass spectra of a control experiment are shown: the spectrum of [5-1H]F420 (Fig 2Aa); the spectrum of [5-1H2]F420H2 generated from [5-1H]F420 by Mtd-catalysed reduction ... respect to C5 of F420 In contrast, auto-oxidation of (5S)-[5-2H1]F420H2 yielded a : mixture of [5-1H]F420 and [5-2H]F420, as indicated by the relative intensities of the 772 and 773 Da mass peaks ... 2005 FEBS H Seedorf et al Stereochemistry of F420H2 oxidase Fig MALDI-TOF-MS analysis of F420 and F420H2 for the determination of the stereospecificity of F420H2 oxidase (FprA) The insets show...

Ngày tải lên: 20/02/2014, 03:20

... (A) MALDI-TOF-TOF mass spectrum of the light (d0) and heavy (d4) precursor ions presenting a mass difference of 4.024 Da (indicated by the curved arrow) (B) Fragmentation mass spectrum of the double-charged ... modified by nondeuterated and deuterated cross-linkers by MALDI-TOF-TOF mass spectrometry A selection of mass spectra illustrates how the comparison of chymotryptic peptides from different cross-linked ... were detected by MS using the 4.0247 atomic mass unit (amu) mass difference conferred by the binding of the nondeuterated or deuterated cross-linkers (Fig 3A) [13,29] Detection of modified peptides...

Ngày tải lên: 15/03/2014, 00:20

... semiquantitive assessment of the relative levels of toxicity of the different mutants was derived by comparing the number of colonies and growth of the spots at the different levels of dilution 4984 CD ... nucleotide is formed by hydrolysis of the 2¢ : 3¢-cyclophosphate group Additional interactions stabilize the initial intermediate of the reaction Following determination of the structure of the complex ... vivo inhibition of Analysis of Kid RNase model cell growth, underlining their relevance to our understanding of the basic activities of this toxin Results Selection and isolation of Kid mutants...

Ngày tải lên: 16/03/2014, 03:20

... enzymatic digestion of proteins to peptides, followed by chromatographic separation of the peptides and electrospray ionization directly into the source of a mass spectrometer The mass spectrometer ... then signals of low abundance will not be selected or 'picked' for sequencing by the instrument The overall protein coverage of the experiment is a function of the sensitivity of the mass spectrometer, ... to the use of more modern mass spectrometric equipment, we were able to identify many more proteins than previous largescale studies Virtually all of the proteins discovered by mass spectrometry...

Ngày tải lên: 14/08/2014, 16:21

... methodology of MS-based proteomics involves the use of a wide variety of 17 separation methods with mass spectrometry (MS), which consists of an ion source for volatizing proteins or peptides, a mass ... measured with certain degree of accuracy, it would be sufficient to help in identifying the protein represented by these peptides The masses of the peptides analysed by the mass spectrometry instrument ... prevention of this disease and improving the current therapies 1.2: Annexin 1.2.1: Structure of ANXA1 ANXA1 is one of the members of the annexin superfamily of 13 members (A1-A13) All members of this...

Ngày tải lên: 10/09/2015, 09:04

... 17294-2:2003(E) Water quality — Application of inductively coupled plasma mass spectrometry (ICP-MS) — Part 2: Determination of 62 elements WARNING — Persons using this part of ISO 17294 should be familiar ... Application of inductively coupled plasma mass spectrometry (ICP-MS) for the determination of elements — Part 1: General guidelines and basic principles Terms and definitions For the purposes of this ... 3.1 limit of application lowest concentration of an analyte that can be determined with a defined level of accuracy and precision Principle Multi-element determination of 62 elements by inductively...

Ngày tải lên: 03/07/2016, 18:45

... analysis of large underivatized peptides (8), and, soon after, of proteins, by the demonstration of the first mass spectrum of insulin in 1982 (9) and of a number of snake toxms with molecular massesup ... discovery by Mickey Barber was the use of a liquid matrix and the technique IS now often termed hquid secondary-ion mass spectrometry (LSIMS) when a primary beam of cesium ions is used Instead of fast ... years, mass spectra of proteins as large as 25 kDa were published using both techniques, concomitantly with the gradual acceptance of the potential of mass spectrometrtc analysts by a number of protein...

Ngày tải lên: 11/04/2014, 10:11

... analyzed Page of 14 (page number not for citation purposes) by MALDI-TOF mass spectrometric analysis In each of the different proteins highlighted, the presence of potential sites of citrullination ... followed by 2-DE analysis, enzymatic digestion of the various citrullinated aldolase obtained and analysis of the peptides by MALDI-TOF MS and by Fourier transform ion cyclotron resonance (FT-ICR) mass ... citrullination of aldolase To verify experimentally the effect of citrullination upon the mass profile observed by MALDI-TOF analysis, we proceeded with the in vitro deimination of aldolase purified...

Ngày tải lên: 09/08/2014, 13:22

... Analysis of complex peptide mixtures by LC-MS2 Peptide mixtures are resolved by liquid chromatography, ionized through electrospray and resolved by MS1 Selected peptides are fragmented by collision ... identification of the same peptides in different LC-MS runs is crucial (Figure 1b) This is achieved by high massaccuracy measurements, and also by aligning different runs based on the LC retention time of ... of mass analyzers (for example, a triple quadrupole MS) ‘filters’ only targeted peptides In combination with isotopelabeled standards, the abundance of peptides is quantitated by comparison of...

Ngày tải lên: 09/08/2014, 20:20

... epilepsy of which ~50% of patients harbor loss -of- function (LOF) mutations in the Epm2a gene that encodes laforin (97-98) 1.3 Research Objectives The objective of this research is to use mass spectrometry ... understanding of the reciprocal regulation of tyrosine phosphorylation by PTKs and PTPs is heavily curbed toward the activities of PTKs for a variety of reasons Apart from the fact that a multitude of PTKs ... half of them have been implicated in the genesis of at least one type of cancer (7) In fact, the regulation of protein tyrosine phosphorylation is controlled by the opposing activities of PTKs...

Ngày tải lên: 24/08/2014, 09:55

... - Bom N2 sach 99.99% - Mày pH Metrohm 646 (Thuy Si) - Phàn xù ly thi su dung cbUdng trình Microsoft Excel va origin Két qua va t h o l u a n Duàng Von-Ampe vàng Obi dUdng Von-Ampe vóng tU -0.2V ... M.D.Luque P.V.A.Prasad, J Arunachalam, Electroanalysis (1994) 589 de Castro, S.Gangadharan, P.Papoff, F.Bocci, N.F.Lanca, Microchem.J 59 (1998) 50 Ishiyama, T Tanaka, Anal.Chem 68 (1996) 3789 10...

Ngày tải lên: 12/03/2015, 14:47

... DEVELOPING SOFTWARE TOOLS FOR STRUCTURE DETERMINATION OF LARGE PROTEINS BY NMR SPECTROSCOPY ZHANG LEI B.SC (HONS.), NUS A THESIS SUBMITTED FOR THE DEGREE OF MASTER OF SCIENCE GRADUATE ... thus far i Table of Contents Acknowledgements i Summary iv List of Tables v List of Figures vi List of Abbreviations and Symbols Chapter 1: Introduction viii 1.1 Basic Principles of NMR 1.2 Spin-Spin ... distal end of side-chains are often involved in mid- to long-range NOEs, more high-quality distance constraints can be obtained for accurate structure determination of large proteins iv List of Tables...

Ngày tải lên: 04/10/2015, 15:45

... (2005) Determination of synthetic chelating agents in surface and waste water by ion chromatography -mass spectrometry, J Chromatogr A, 1085, 240-246 Nishikawa Y and Okumura T (1995) Determination of ... for the Examintion of Water would be to perform an inter-laboratory validation study of the proposed method Determination of EDTA in river water samples For the application of this analytical ... the Examination of Water Occurrence of EDTA in river water samples from three regions of Japan was examined EDTA was detected in ten of thirteen river water samples A high level of EDTA was observed...

Ngày tải lên: 05/09/2013, 10:15

... The raw data sets of the homonuclear 2D experiments typically consisted of 4K · 256 complex data points Results Determination of branching pattern and blood group determinants by ES-MS/MS Negative-ion ... linkage is supported by an rOe between GalVIII H1 and NAc protons of GlcNAc VII Extension of the 6-branch is by a GalIVb1–4GlcNAcIII linkage, as GalIV H1 gives an rOe to H4 of GlcNAc III Gal IV ... shifts of the structural reporter protons of GlcNAc III are almost identical with those reported for the GlcNAcb1–6 residue of lacto-N-hexaose [18] Ó FEBS 2004 Sequence determination of oligosaccharides...

Ngày tải lên: 19/02/2014, 12:20

... 10-80% of the base peak Low- to mid -mass sensitivity 197 50% of Mass 442 Mid -mass resolution and sensitivity 199 5-9% of Mass 198 Mid -mass ... (DECAFLUOROTRIPHENYLPHOSPHINE, DFTPP) Mass (M/z) Relative Abundance Criteria Purpose of Checkpoint1 51 10-80% of the base peak Low -mass sensitivity 68 50% of Mass 198 High -mass resolution...

Ngày tải lên: 22/03/2014, 14:20

... more sources of errors The specificity of the MS detection ensures an unequivocal determination of the analysed substances Page of (page number not for citation purposes) Journal of Occupational ... including the recondition step of the column for the next injection, was 21 Figure represents an example of a chromatogram of spiked EBC with each analyte Mass spectrometry and internal standardization ... detection of multiple analytes is possible However, the determination of these kinds of markers in breath condensate makes a sample enrichment step unavoidable when attempting to achieve a low limit of...

Ngày tải lên: 20/06/2014, 00:20

... tissues by LC-MS has been established The limit of detection and limit of quantitation were ng/g and ng/g These values satisfied the acceptance criteria of the limit of detection and limit of quantitation ... linear regression analysis of the log-transformed tissue concentrations, and was determined at the time when the Determination of Roxithromycin by Liquid Chromatography /Mass Spectrometry after Multiple-Dose ... detected or under LOQ Determination of Roxithromycin by Liquid Chromatography /Mass Spectrometry after Multiple-Dose Oral Administration in Broilers 39 Ta ble Concentration of roxithromycin in various...

Ngày tải lên: 07/08/2014, 17:22

... analysis of such solvents, it is essential that the vapor pressure is controlled by cooling the spray chamber, where the sample aerosol is generated This can be best affected by means of a Peltier ... the vapor pressure of even the most volatile solvent (such as acetone) is sufficiently reduced to allow stable plasma operation Removal of carbon The presence of high levels of organic solvent ... considerably more reactive, and so the use of platinum-tipped interface cones instead of the standard nickel cones is recommended Optimization of the appropriate level of oxygen for a particular organic...

Ngày tải lên: 05/03/2014, 21:20