determination of antibody affinity by surface plasmon resonance

... sensing of single binding events by localized surface plasmon resonance Nano Lett 2008, 8:3450-3455 11 Scarano S, Mascini M, Turner AP, Minunni M: Surface plasmon resonance imaging for affinity- based ... regenerated by washing with 100 μL of 0.01% SDS and 100 μL of mM HCl at a speed of 50 μL/min To equilibrate the chip surface, system buffer was supplemented at a speed of 200 μL/min for 30 Detection of ... laboratory detection of bacterial meningitis Am J Med 1983, 75:119-123 Nakatani K, Sando S, Saito I: Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat...

Ngày tải lên: 18/06/2014, 19:20

... sensing of single binding events by localized surface plasmon resonance Nano Lett 2008, 8:3450-3455 11 Scarano S, Mascini M, Turner AP, Minunni M: Surface plasmon resonance imaging for affinity- based ... regenerated by washing with 100 μL of 0.01% SDS and 100 μL of mM HCl at a speed of 50 μL/min To equilibrate the chip surface, system buffer was supplemented at a speed of 200 μL/min for 30 Detection of ... laboratory detection of bacterial meningitis Am J Med 1983, 75:119-123 Nakatani K, Sando S, Saito I: Scanning of guanine-guanine mismatches in DNA by synthetic ligands using surface plasmon resonance Nat...

Ngày tải lên: 20/06/2014, 03:20

... amount of LMW heparin or water (controls) Thus, the differences in the positions of the fitted straight lines are indicative of the amount of bound peptide It is clear that binding of heparin by the ... AP-1), the data cover only a limited part of the binding curve because of peptide solubility problems and because of the limits of detection offered by UV-based detection in CE The data therefore ... rate of lLÆmin)1 (over a concentration range of 10–300 lM) At the end of each sample injection, the same buffer was continuously flowed over the sensor surface to monitor dissociation At the end of...

Ngày tải lên: 31/03/2014, 23:20

... 12 Hutter E, Fendler JH: Exploitation of localized surface plasmon resonance Adv Mater 2004, 16:1685 13 Willets KA, Van Duyne RP: Localized surface plasmon resonance spectroscopy and sensing Annu ... Biosensing with plasmonic nanosensors Nat Mater 2008, 7:442 15 Xiang G, Zhang N, Zhou X: Localized surface plasmon resonance biosensing with large area of gold nanoholes fabricated by nanosphere ... size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy Nanoscale Research Letters 2011 6:336 Page of Submit your...

Ngày tải lên: 21/06/2014, 04:20

... conditions by different methods varied by as much as two orders of magnitude [12,13,17] We therefore decided to investigate membrane binding by surface plasmon resonance With this method the kinetics of ... Characterization of the surface generated by liposome binding to the modified dextran matrix of a surface plasmon resonance sensor chip An Biochem 280, 29–35 Abbott, A.J & Nelsestuen, G.L (1987) Association of ... AB koff;1 kon;2 AB þ B )* AB2 koff;2 ð2Þ ð3Þ where d½BŠ=dt ¼ À 2kon;1 ½AŠ½BŠ þ koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2 ½AB2 Š ð4Þ d½ABŠ=dt ¼ 2kon;1 ½AŠ½BŠ À koff;1 ½ABŠ À kon;2 ½ABŠ[B] þ 2koff;2...

Ngày tải lên: 08/03/2014, 23:20

... parameters of molecular modeling software package SYBYL 6.8 (http://www.tripos.com) The geometries of the ligands were subsequently optimized by using the Power method encoded in SYBYL 6.8 to ... plasmid were grown in LB medium containing 50 lgặmL)1 of ampicillin at 37 C The expression of PPARcLBD was induced by the addition of 0.2 mM of isopropyl b-D-thiogalactoside (IPTG) After induction ... is the rst report of use of CD to detect the conformational change and to monitor the Tm of PPARc unfolding The result indicated that the thermal stability of PPARcLBD enhanced by the ligand binding,...

Ngày tải lên: 16/03/2014, 16:20

... effect, namely, increase of IQE via emitter /plasmon coupling and increase of light extraction efficiency [LEE] by means of out-coupling of the generated photons Enhancement of IQE Figure Enhanced ... LED by mediating surface plasmon polaritons Proc SPIE 2008, 7032:70320R 24 Gifford DK, Hall DG: Emission through one of two metal electrodes of an organic light-emitting diode via surface- plasmon ... Exploitation of localized surface plasmon resonance Adv Mater 2004, 16:1685 52 Eustis S, El-Sayed MA: Why gold nanoparticles are more precious than pretty gold: nobel metal surface plasmon resonance...

Ngày tải lên: 21/06/2014, 05:20

... wet-etched surface, with the relationship of 0 xh À xh 2  2  2 0 þ yh À yh þ zh À zh ¼ te2 P0 is also on the normal to the surface expressed by Eq Based on Eqs and 8, P0 can be calculated by ,sffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi ... Continuous wet etching of gold nanostructures in the undiluted etchant a is SEM image of the sample before wet etching, which is obtained by evaporating 50 nm of gold onto the nanospheres of 175 nm in ... nanospheres in Fig were not well dispersed, by optimizing the concentration of the nanospheres by diluting it to a ratio of 1:15, better dispersion of silica nanospheres on glass substrate was...

Ngày tải lên: 22/06/2014, 00:20

... surface was smoothed using a × × averaging of voxels (downsampling) Using the ‘Create Surface function of Amira, a surface was derived from the isosurface, which allowed measurement of the surface ... voltage of 120 kV Series of micrographs (tilt-series) were recorded semiautomatically assisted by the Xplore 3D tomographymodule of the microscope control software (FEI) over a tilt range of at ... The surface area and volume of individual spherical particles were approximated by the formulas to calculate the surface area (4πr ) and the volume (4/3πr ) of a Van Doren et al Journal of Nanobiotechnology...

Ngày tải lên: 11/08/2014, 00:23

... oscillations of a particular frequency known as the resonance frequency The resonance frequency changes as the thickness of the surface of the crystal changes As the mass of the surface changes, the resonance ... of Hepatitis C virus aptamers88; 89 SPR is fast becoming the method of choice for the determination of binding affinity9 0 This is due to the fact that it allows for real time determination of ... the determination of binding affinity of orchatoxin aptamers 94 Gel electrophoresis which has demonstrated the highest binding affinity range reported of KD 10-13 M was used in the validation of...

Ngày tải lên: 10/09/2015, 09:22

... fabrication by LIL 1.3.2 Large-area nanoparticle array fabricated by colloidal lithography 1.3.3 Tuning of surface plasmon resonance by sphere size and film thickness 1.3.4 Tuning of surface plasmon resonance ... Theoretical Background 2.1 Fundamentals of surface plasmons (SPs) 2.1.1 Basic properties of surface plasmons Surface plasmon polaritons (SPPs), often referred to as surface plasmons (SPs), are resonant ... potential of allowing us to develop industrial applications by incorporating plasmonic resonance tuning 1.3.4 Tuning of surface plasmon resonance by bimetallic structures To the best of our knowledge,...

Ngày tải lên: 16/10/2015, 11:57

... internally reflecting base of the prism to the bound surface field of the surface plasmons Experimentally, the resonance is observed by monitoring the reflected light as a function of the incident angle ... Gratings fabricated by these two methods could be used for the surface plasmon coupling The process of PANI / SPANI multilayer film manipulated on the Au surface by the Layer -by- Layer technique ... the excitation of surface plasmons in SPS Fig 3.3 Comparison of angular and kinetic scans during adsorption of additional layer Fig 3.4 Sketch of the cell in EC-SPS Fig 3.5 Sketch of an evaporation...

Ngày tải lên: 08/11/2015, 17:00

... “Approximate model for surface- plasmon generation at slit apertures,” J Opt Soc Am A 23(7), 1608–1615 (2006) 16 L Zhang, A Kubo, L Wang, H Petek, and T Seideman, “Imaging of surface plasmon polariton ... “Gate-tuning of graphene plasmons revealed by infrared nano-imaging,” Nature 487(7405), 82–85 (2012) 21 A Huber, N Ocelic, D Kazantsev, and R Hillenbrand, “Near-Field Imaging of Mid-Infrared Surface ... a small region of tip-sample interaction, which is of the order of tens of nanometers [9] Several methods have been proposed to suppress the background, for instance, regulation of tip sample...

Ngày tải lên: 06/05/2014, 08:54

... “Approximate model for surface- plasmon generation at slit apertures,” J Opt Soc Am A 23(7), 1608–1615 (2006) 16 L Zhang, A Kubo, L Wang, H Petek, and T Seideman, “Imaging of surface plasmon polariton ... “Gate-tuning of graphene plasmons revealed by infrared nano-imaging,” Nature 487(7405), 82–85 (2012) 21 A Huber, N Ocelic, D Kazantsev, and R Hillenbrand, “Near-Field Imaging of Mid-Infrared Surface ... a small region of tip-sample interaction, which is of the order of tens of nanometers [9] Several methods have been proposed to suppress the background, for instance, regulation of tip sample...

Ngày tải lên: 06/05/2014, 08:58

... luminescence of GaN-based light-emitting diode with a localized surface plasmon resonance Microelectron Eng 2009, 86:1120-1123 Lepage D, Dubowski JJ: Surface plasmon effects induced by uncollimated ... would yield a surface roughness of 15 Å From these numbers, we can estimate that at a given energy, the contribution of surface roughness to SPs loss in scattering is reduced by a factor of 13 × [9] ... Burke JJ, Stegeman GI, Tamir T: Surface- polariton-like waves guided by thin, lossy metal films Phys Rev B 1986, 33:5186 Mills DL: Attenuation of surface polaritons by surface roughness Phys Rev B...

Ngày tải lên: 21/06/2014, 03:20

... the simultaneous determination of macrolides The fragmentation patterns provide the confirmatory information of macrolides The relevance of these studies for the determination of macrolide in ... curves have been constructed by plotting area against the standard concentrations of macrolides in the range of 0.001 ㎍/㎖ ~ ㎍/㎖ Limit of detection (LOD) and limit of quantitation (LOQ) were based ... an d We lfare Official Methods for Residual Substances in Livestock Products, Ministry of Health and Welfare, J apan, 1994 AOAC In te rn a tion a l Official Methods of Analysis of AOAC International...

Ngày tải lên: 07/08/2014, 15:20

... reproducibility of patients' setup by using the video -surface imaging reg- Figure Alignment of a daily image to the reference surface image Alignment of a daily image to the reference surface image Page of ... that brings the surface model of the daily treatment fraction into congruence with the reference surface The verification of patient setup can be performed by a number of methods of varying sophistication, ... calculated by means of Align RT were compared with the setup errors detected by the EPID images Figure treatment room surface registration system, mounted on the ceiling of the Photograph of the...

Ngày tải lên: 09/08/2014, 09:22

... LP, Huang LS, Lin CW, Lee CK, Chen JL, Hsu SM, Lin S: Determination of binding constant of DNA-binding drug to target DNA by surface plasmon resonance biosensor technology Curr Drug Targets Immune ... experiments Tsai et al Journal of Biomedical Science 2010, 17:49 http://www.jbiomedsci.com/content/17/1/49 Page of Figure Surface plasmon resonance sensorgram of the interaction between immobilized ... combining the amount of DNA in the tail with the distance of migration of 50 analyzed cells, were calculated using image analysis software (Comet Assay Software Project, http:// www.casp .of. pl/) Tail...

Ngày tải lên: 10/08/2014, 05:21

... - Bom N2 sach 99.99% - Mày pH Metrohm 646 (Thuy Si) - Phàn xù ly thi su dung cbUdng trình Microsoft Excel va origin Két qua va t h o l u a n Duàng Von-Ampe vàng Obi dUdng Von-Ampe vóng tU -0.2V ... M.D.Luque P.V.A.Prasad, J Arunachalam, Electroanalysis (1994) 589 de Castro, S.Gangadharan, P.Papoff, F.Bocci, N.F.Lanca, Microchem.J 59 (1998) 50 Ishiyama, T Tanaka, Anal.Chem 68 (1996) 3789 10...

Ngày tải lên: 12/03/2015, 14:47

... improvement in the study of surface plasmon With these methods, surface plasmons were easily generated by many researchers Till this time, although the properties of surface plasmon were well observed, ... 1968, the behavior of metal gratings7 where surface plasmon resonances were excited was described by Ritchie In the same year, surface plasmon was optically excited on metal film8 by Andreas Otto ... 1.4 Scope of thesis .19 Chapter 2: Theory 22 2.1 Principle of Surface Plasmon 22 2.11 Non-localized surface plasmon 29 2.12 Localized surface plasmon ...

Ngày tải lên: 09/09/2015, 11:16