detection of herpes simplex virus in csf by pcr

... performance of two real-time PCR assays for detection of herpes simplex virus J Clin Microbiol 2005, 43:2391-2398 Ashley RL: Laboratory techniques in the diagnosis of herpes simplex infection Genitourin ... questionnaire and an examination for genital lesion by the clinician/nursing officer The study was approved and Detection of HSV DNA, probe typing & melting point determination Quantitative PCR was performed ... 1) Of the samples confirmed by Rotorgene, of these had no mutations in the probe binding area The remaining samples resulted in poor quality sequencing reactions and the results were not interpretable...

Ngày tải lên: 19/06/2014, 08:20

... performance of two real-time PCR assays for detection of herpes simplex virus J Clin Microbiol 2005, 43:2391-2398 Ashley RL: Laboratory techniques in the diagnosis of herpes simplex infection Genitourin ... questionnaire and an examination for genital lesion by the clinician/nursing officer The study was approved and Detection of HSV DNA, probe typing & melting point determination Quantitative PCR was performed ... 1) Of the samples confirmed by Rotorgene, of these had no mutations in the probe binding area The remaining samples resulted in poor quality sequencing reactions and the results were not interpretable...

Ngày tải lên: 20/06/2014, 04:20

... STING Consistent with our in vitro findings, in vivo HSV-1 infection failed to elicit significant changes in RIP3 expression by either cell type Interestingly, and in contrast to our studies in ... Quantitation of herpes simplex virus type DNA in cells of cerebrospinal fluid of patients with herpes simplex virus encephalitis Neurology 1997, 48:1341-1346 Trifilo MJ, Lane TE: Adenovirus-mediated ... Markowitz LE: Trends in herpes simplex virus type and type seroprevalence in the United States JAMA 2006, 296:964-973 Baringer JR: Herpes Simplex Infections of the Nervous System Neurol Clin 2008, 26:657-674...

Ngày tải lên: 19/06/2014, 22:20

... role of polymorphonuclear leukocyte infiltration in herpes simplex virus infection of murine skin Arch Dermatol Res 1999, 291:28-36 Milligan GN: Neutrophils aid in protection of the vaginal mucosae ... lethal influx of extracellular Ca2+ into the cells [26] We also indicated that treatment of HSV-1-infected epithelial cells with ionomycin induced the increase of Ca2+ influx, followed by cell ... is induced by rotavirus infection through an increase in intracellular Ca2+ concentration Virology 1998, 252:17-27 Shainkin-Kestenbaum R, Winikoff Y, Chaimovitz C, Zimlichman S, Sarov I: Inhibitory...

Ngày tải lên: 20/06/2014, 01:20

... signals in herpes simplex virus type thymidine kinase Biochem Biophys Res Commun 1999, 264:338-342 Söling A, Simm A, Rainov NG: Intracellular localization of Herpes simplex virus type thymidine kinase ... likely a result of steric hindrance and might be substantially reduced by selecting another linker sequence Longer intervening sequences as well as introduction of flexible polyglycine linkers may ... (during and after GCV therapy) of tumor volumes and integrated light intensity units within a region of interest (ROI) including all tumors were plotted against each other (Figure 5) Within the...

Ngày tải lên: 14/08/2014, 19:22

... changes in its conformation [15] The HSV-induced membrane fusion is accomplished by initially bringing the membranes of both the host cell and virus into close contact by receptor ⁄ glycoprotein interactions ... nectin-1 in herpes simplex virus entry and spread in human retinal pigment epithelial cells FEBS J 275, 5272–5285 Dixit R, Tiwari V & Shukla D (2008) Herpes simplex virus type induces filopodia in ... assisting the entry of herpes simplex virus type Biochem J 385, 451–459 Yoon M, Zago A, Shukla D & Spear PG (2003) Mutations in the N termini of herpes simplex virus type and gDs alter functional interactions...

Ngày tải lên: 23/03/2014, 04:20

... concentrations (2.5% ethyl acetate plus virus) , examining the effectiveness of a water extract of the plant, or elucidating the mechanism of synergistic cytotoxicity of ethanol and the compound It is ... heptaphylla in high yield (0.7 ÷ 0.8%) The structure of the compound was determined by interpretation of its spectral data (IR, MS, 1H and 13C-NMR) and by comparison with spectral data of an authentic ... limit of solubility in water (with 0.85% NaCl) Further studies should focus on examining the compound at lower concentrations (0.5 mg/ml down to 0.05 mg/ml or lower), examining the role of solvent...

Ngày tải lên: 03/04/2014, 15:20

... showed induction of MIP-2, MIP-1α, and MCP-1 chemokines in the cornea during acute infection Infection of mouse fibroblast cells by HSV induces expression of IL-6 [18], and infection of macrophages ... Rouse BT: Herpes simplex virus replication-induced expression of chemokines and proinflammatory cytokines in the eye: implications in herpetic stromal keratitis Journal of Interferon & Cytokine Research ... http://www.virologyj.com/content/1/1/5 A striking finding in this analysis was the persistent expression of inflammatory cell RNAs during the latent phase of TG infection when detectable production of infectious virus has ceased...

Ngày tải lên: 18/06/2014, 22:20

... transcripts of herpes simplex virus: RNA in search of function Virology 1992, 191:1-8 Lee LY, Schaffer PA: A virus with a mutation in the ICP4-binding site in the L/ST promoter of herpes simplex virus ... cause of death in KOS-GFP-infected ifnar-/ - ifngr-/- mice Loss of in vivo alleviates innate host repression of ICP0Figure viruses Stat Loss of Stat alleviates innate host repression of ICP0- viruses ... were inoculated by scarifying the cornea with a 26-gauge needle and by placing µl complete DMEM containing × 105 pfu of virus on each eye Viral titers in the ocular tear film of mice were determined...

Ngày tải lên: 20/06/2014, 01:20

... The titre of HI antibodies in the serum was determined to define whether the individuals had suffered primary or secondary infections HI antibody titre of >1:2560 in the acute phase of infection ... confirmatory of secondary infection [8] Case had very low levels of HI antibodies (1:40 to 1:80) indicating a primary infection Case had HI titre of 1:2560 indicating a secondary infection The ... analysed in context with other sequences of DENV-4, present in the GenBank using Maximum Likelihood method, PhyML_3.0 [9] To date, four genotypes of DENV-4 virus have been described consisting of viruses...

Ngày tải lên: 11/08/2014, 21:22

... Amplicon melting analysis of fragment ABD6-1 in 32 blind Amplicon melting analysis of fragment ABD6-1 in 32 blind samples Amplicon melting analysis of fragment ABD6-1 in 32 blind samples, showing five ... populations contain singlenucleotide changes in the genome To detect such induced mutations in a PCR reaction end-point containing fragments of three homoeoloci in wheat means the software should ... determined by comparing the ratio of two overlapping peaks with that of neighboring SNPs (intrinsic SNPs among three loci) and a mutation score greater than seven as reported by Mutation Surveyor® PCR...

Ngày tải lên: 12/08/2014, 03:21

... mutagenesis, was generated by cloning a 773 bp DNA fragment containing the UL20 gene, obtained by PCR amplification of HSV-1(KOS) viral DNA, into pCR2 .1/TOPO (Invitrogen) as described in detail previously ... domain spanning amino acids 204–211is important for both intracellular transport and virus- induced cell fusion Domain I is the largest domain (63 aa) and it includes stretches of acidic amino ... localization However, mutagenesis of both Y38 and Y49 amino acid residues in the amino terminus of UL20p, inhibited virus- induced cell fusion, while allowing efficient intracellular transport and TGN...

Ngày tải lên: 18/06/2014, 18:20

... the eyes, and by placing µl of complete DMEM containing × 105 pfu/eye of virus on each eye Viral titers were determined by swabbing the ocular surface of both eyes at times after inoculation with ... anaesthetized by intraperitoneal (i.p.) administration of xylazine (6.6 mg/kg) and ketamine (100 mg/kg) Mice were inoculated by scarifying the cornea with the blunted tip of a 25-gauge needle, blotting ... role in modulating resistance against herpes simplex virus Curr Top Microbiol Immunol 1992, 179:89-110 Engler H, Zawatzky R, Kirchner H, Armerding D: Experimental infection of inbred mice with herpes...

Ngày tải lên: 19/06/2014, 08:20

... domain Fas apoptotic inhibitory molecule Helicase lymphoid specific Interleukin 10 MAP kinase interacting protein C3HC type zinc finger protein NF-κB Ring finger protein P21 (CDKN1A)-activated kinase ... repeat-containing BCL2/adenovirus E1B-interacting protein Apoptosis inhibitor TSC22 domain family Cell-death inducing DNA fragmentation factor CASP2 and RIPK1 adaptor domain containing protein Fas-associated ... Journal of Neuroinflammation 2007, 4:11 Herpes simplex virus (HSV-1) is a neurotropic virus that infects a wide range of mammalian cells Following primary infection of epithelial cells, HSV gains...

Ngày tải lên: 19/06/2014, 22:20

... the eyes, and by placing µl of complete DMEM containing × 105 pfu/eye of virus on each eye Viral titers were determined by swabbing the ocular surface of both eyes at times after inoculation with ... anaesthetized by intraperitoneal (i.p.) administration of xylazine (6.6 mg/kg) and ketamine (100 mg/kg) Mice were inoculated by scarifying the cornea with the blunted tip of a 25-gauge needle, blotting ... role in modulating resistance against herpes simplex virus Curr Top Microbiol Immunol 1992, 179:89-110 Engler H, Zawatzky R, Kirchner H, Armerding D: Experimental infection of inbred mice with herpes...

Ngày tải lên: 20/06/2014, 04:20

... JA: Induction and prevention of apoptosis in human HEp-2 cells by herpes simplex virus type J Virol 1999, 73:10359-10370 Aubert M, Blaho JA: Modulation of apoptosis during herpes simplex virus infection ... expression of any of the Bax isoforms in the mockinfected SIRC cells (Fig 4) Interestingly, we observed a dramatic rise in the level of Bax-β in HSV-1-infected cultures (Fig 4) Following the demonstration ... manipulation of cell death Curr Pharm Des 2008, 14:198-220 33 Megyeri K: Modulation of apoptotic pathways by herpes simplex viruses In Latency Strategies of Herpesviruses 1st edition Edited by: Minarovits...

Ngày tải lên: 10/08/2014, 05:21

... rapid induction of cell fusion by virions in the absence of viral protein synthesis [12] HSV-1 ANG path is a prototype FFWO strain The combination of two amino acid mutations in gB, one in the ... without induced by herpes simplex virus type Intervirology 1985, 24:211-219 13 Saharkhiz-Langroodi A, Holland TC: Identification of the fusion-fromwithout determinants of herpes simplex virus type ... entry of herpes simplex virus into the cell Rev Med Virol 2007, 17:313-326 Nicola AV, McEvoy AM, Straus SE: Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese...

Ngày tải lên: 12/08/2014, 02:20

... members, is one of the main HECT E3 protein families They are characterized by a unique domain architecture, with an amino-terminal C2 domain, two to four proteinprotein interacting WW domains and a ... predominantly to trans-Golgi network in HSV-2 infected cells We further investigated detailed intracellular localization of UL56 in infected cells using immunostaining Infected cells contain abundant ... is incorporated into virions or remains in the limiting membrane of the vesicles UL56 protrudes into the cytoplasm from the membrane of vesicles containing virions, and interacts with proteins...

Ngày tải lên: 12/08/2014, 04:20