detection of app intracellular domain in brain tissue
Báo cáo khoa học: Increased glucose metabolism and ATP level in brain tissue of Huntington’s disease transgenic mice pdf
Ngày tải lên :
23/03/2014, 10:20
... acids of human huntingtin with 82 polyglutamine repeats exclusively in brain, with the level of the transgene product remaining lower than the level of the endogenous full-length huntingtin [24] ... bobbing, head tilting and circling The levels of the endogenous (wild-type) huntingtin protein and the transgene product were examined by western blotting using anti-huntingtin serum raised against ... concentrations in the posterior regions of intact brain in normal and HD mice The availability of an in silico representation of the glycolytic pathway in both normal and HD brain offers the potential...
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Báo cáo khoa học: "Detection of Agreement and Disagreement in Broadcast Conversations" pot
Ngày tải lên :
07/03/2014, 22:20
... 40.1 51.2 trying to balance the class distribution in the training set by either oversampling the minority class or downsampling the majority class In this preliminary study of sampling approaches ... Proceedings of HLT/NAACL 378 D Hillard, M Ostendorf, and E Shriberg 2003 Detection of agreement vs disagreement in meetings: Training with unlabeled data In Proceedings of HLT/NAACL A Janin, D ... composed of different combinations of initiating utterances and responses We reformulated the (dis)agreement detection task as the sequence tagging of 11 (dis)agreement-related labels for identifying...
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Báo cáo khoa học: Mutational analysis of the C-domain in nonribosomal peptide synthesis pot
Ngày tải lên :
17/03/2014, 11:20
... activity and selectivity of the A domain embedded within the C- and T-domains of all tridomain TycB1ProCAT derivatives Although A domain activity indicates a correct folding of the entire TycB1Pro-CAT ... C-domains possess an intrinsic editing function for the incoming aminoacyl moiety [6] By using aminoacyl-S-CoA as probes it was shown that the ®rst C -domain of the tyrocidine synthetase complex ... chromosomal point mutation in srfA-B, changing a D to A in the His-motif (italic) of the corresponding Asp domain of the surfactin synthetase was found to abolish surfactin production in the B subtilis...
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Báo cáo khoa học: Identification and functional characterization of an aggregation domain in long myosin light chain kinase ppt
Ngày tải lên :
30/03/2014, 04:20
... and in intracellular cytoskeletal proteins, including titin, MyBP-Cm, MyBP-M, MyBPH, myotilin, and palladin [30–35] As Ig-C2 domains may serve as molecular spacers and bind to a diversity of ligands, ... domains are at particular risk of forming intractable aggregates [37–39] Studies on at least two amyloidal diseases involving deposition of Ig domains support the notion that Ig domains are involved ... barrier in intestinal disease Lab Invest 84, 282–291 12 Tran QK, Watanabe H, Zhang XX, Takahashi R & Ohno R (1999) Involvement of myosin light-chain kinase in chloride-sensitive Ca2+ in ux in porcine...
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Báo cáo sinh học: "Conservation of core gene expression in vertebrate tissues" pdf
Ngày tải lên :
06/08/2014, 19:20
... Antithrombin-III precursor (ATIII) Fibrinogen gamma chain precursor Uncharacterized protein Fibrinogen beta chain precursor Plasma retinol-binding protein precursor (PRBP) Inter-alpha-trypsin inhibitor ... genes within the set of unique orthologs were defined by the presence of a DNA-binding domain in the mouse protein sequence in the Pfam database [75] Our list of TF genes is given in Additional ... P-Liver H-Pancreas H-Stomach M-Large intestine M-Small intestine M-Stomach C-Gallbladder P-Gallbladder C-Intestine P-Intestine P-Stomach F-Smallintestine F-Largeintestine F-Stomach C-Oviduct C-Stomach...
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Báo cáo khoa học: "Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections" pdf
Ngày tải lên :
07/08/2014, 17:22
... Okjin Kim Table Comparison of different protocols for the detection of canine herpesvirus DNAs in paraffin sections by in situ hybridization Labelsb Protease Kc Hybridizationd 100 Titer of inoculated ... differences in the detection limit of ISH However, digestion with 200 µg/ml proteinase K caused some tissue degradation and increased background staining Digestion with 100 µg/ml proteinase K induce ... methods for the detection of intracellular viral DNAs in paraffin sections J Vet Med Sci 2003, 64, 231-235 73 Kim O, Chae C In situ hybridization for the detection and localization of porcine epidemic...
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Báo cáo y học: "Early Detection of cytokine protein expression in mouse lung homogenates using suspension bead array" ppt
Ngày tải lên :
11/08/2014, 08:21
... Journal of Inflammation 2006, 3:15 inflammatory cytokines, interleukin-6 (IL-6) and macrophage inflammatory protein-2 (MIP-2) protein levels in mouse lung tissue homogenates are increased following ... diagnostic markers indicative of the LPS-evoked lung inflammatory injury response While, traditional immunohistochemical staining methods for detection of cytokine protein expression in tissue sections ... mice Representative histology of LPS-induced lung injury in CD-1 mice H&E staining of left lung lobe from a female mouse following a single i.p injection of 0.2 ml of 0.25 mg/ml LPS for hours (B)...
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báo cáo khoa học: " High resolution melting analysis for the detection of EMS induced mutations in wheat SbeIIa genes" doc
Ngày tải lên :
11/08/2014, 11:21
... relationship in the targeted enzyme SBEs are found to contain three domains: an amino-terminal domain, a carboxyl-terminal domain and a central catalytic domain [27,34] The N-terminal region is Page of ... and in the protein secondary structure In Figure we show as an example the case of SBEIIa-D (V398I) : while in wild type protein residue 398 was involved in the b3 strand of the (a/b)8 domain, in ... tolerance of mutations resulting in a higher density in the population This offers the possibility of identifying a wide variety of mutations in the target genes by screening a realistic number of mutagenised...
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Báo cáo y học: " Detection of dengue-4 virus in pune, western india after an absence of 30 years - its association with two severe cases" docx
Ngày tải lên :
11/08/2014, 21:22
... The titre of HI antibodies in the serum was determined to define whether the individuals had suffered primary or secondary infections HI antibody titre of >1:2560 in the acute phase of infection ... confirmatory of secondary infection [8] Case had very low levels of HI antibodies (1:40 to 1:80) indicating a primary infection Case had HI titre of 1:2560 indicating a secondary infection The ... The high degree of diversity in the envelope gene observed for the DENV-4 viruses circulating on the subcontinent indicates that the serotype is evolving and that close monitoring of the serotype...
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Báo cáo y học: "Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma" pdf
Ngày tải lên :
12/08/2014, 01:22
... measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 ... http://www.virologyj.com/content/7/1/253 Page of 10 Figure Detection of HPV infection by PCR in OSMF and OSCC cases Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF ... the sample/mean of RLU of three positive controls (PC) were taken as an estimate of approximate viral load which relates to the index of the intensity of HPV infection This ratio of any specimen...
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báo cáo khoa học: " Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®" pot
Ngày tải lên :
12/08/2014, 03:21
... Amplicon melting analysis of fragment ABD6-1 in 32 blind Amplicon melting analysis of fragment ABD6-1 in 32 blind samples Amplicon melting analysis of fragment ABD6-1 in 32 blind samples, showing five ... populations contain singlenucleotide changes in the genome To detect such induced mutations in a PCR reaction end-point containing fragments of three homoeoloci in wheat means the software should ... especially within the C-terminal domain The method presented here is effective in detecting mutations in this region in a TILLING population although false positives are detected by independent...
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Báo cáo y học: " Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys" pps
Ngày tải lên :
12/08/2014, 04:21
... 40-70% in most of the epithelial cells of large intestine in the bird species (Table 1) We did not observe the presence of either type of receptors in the epithelium of small intestine of 2-weekold ... were observed in small or large intestine Differences in distribution of receptors in other tissues examined In day-old birds, the tubular cells of the kidney showed positive staining for a2,3SA-gal ... intermediate hosts in the interspecies transmission of influenza viruses [14] Equal intensity of strong positive staining for avian and human type receptors observed in the trachea of ducks of...
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Báo cáo y học: "Detection of Chlamydia trachomatis-DNA in synovial fluid: evaluation of the sensitivity of different DNA extraction methods and amplification systems" pps
Ngày tải lên :
12/08/2014, 11:22
... trachomatis in SF In the inflamed joint, Chlamydia persists intracellularly in monocytes [4,27], which is the reason why the analysis of C trachomatis EB is not fully comparable with the clinical in vivo ... Table Sensitivity of PCR for the detection of intracellular persisting Chlamydia trachomatis in synovial fluid depending on DNA extraction method Number of serial dilution Alkaline lysis Qiaex ... However, testing for detection of C trachomatis DNA in SF has not yet been standardized accordingly for use in clinical practice Laboratories employ different in- house methods for preparation of template...
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Báo cáo y học: " Isolation of human β-defensin-4 in lung tissue and its increase in lower respiratory tract infection" ppt
Ngày tải lên :
12/08/2014, 18:20
... normal lung tissues, hBD-4 immunostaining in lung tissue of chronic LRTI was observed in the bronchial lumen as well as in the cytoplasm of epithelial cells Also, IHC and ELF findings suggested ... with high affinity at final dilutions of 1/2,100,000 (35% binding) Half-maximum inhibition occurred at pg/tube The peptide remained detectable at the low level of 0.7 pg/tube At 50% binding, the ... suppuration in case and lung nodule of unidentified etiology in cases According to the results of the histological study, laboratory data, clinical course, and radiological findings including positron...
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Báo cáo khoa học: " Evaluation of three commercial bovine ELISA kits for detection of antibodies against Alphaherpesviruses in reindeer (Rangifer tarandus tarandus)" pdf
Ngày tải lên :
12/08/2014, 18:22
... ruminant species Of the alphaherpesviruses infecting ruminants bovine herpesvirus type (BoHV-1), causing the diseases Infectious Bovine Rhinotracheitis (IBR) and Infectious Pustular Vulvovaginitis (IPV), ... semi-nomadic way being herded between summer and winter pastures, and being usually free-ranging within the borders of their specific herding districts Mortality rates in reindeer in Finnmark vary ... panel of reindeer serum samples tested in three commercial bovine ELISA kits for detection of Serial dilution curves of a panel of reindeer serum samples tested in three commercial bovine ELISA...
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Báo cáo y học: " Detection of a gammaretrovirus, XMRV, in the human population: Open questions and implications for xenotransplantation" ppsx
Ngày tải lên :
12/08/2014, 23:23
... contributory factor in the pathogenesis of CSF [10] In light of these results, the finding of XMRV in prostate tumours and CFS patients in the USA has to be treated with great caution In the past, numerous ... be linked to human diseases Highly sensitive methods such as PCR allow detection of minimal traces of retroviruses but also detect sample contamination Indeed, contaminating retroviruses, mainly ... miniature swine (MMS) Troll Vet Microbiol 2007, 20;123(1-3):53-68 Martin SI, Wilkinson R, Fishman JA: Genomic presence of recombinant porcine endogenous retrovirus in transmitting miniature swine Virol...
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Báo cáo khoa học: "Rapid detection of pneumothorax by ultrasonography in patients with multiple trauma" pptx
Ngày tải lên :
13/08/2014, 02:24
... absent lung-sliding appeared alternately The size of pneumothorax was inferred by ascertaining such points at different intercostal spaces When these points are lined up, the contour of the pneumothorax ... The results of chest CT and radiography were interpreted by independent radiologists who were unaware of patients' conditions and the findings of US In patients with clinical suspicion of large ... Determination of the size of pneumothorax and its clinical significance Determination of the size of a pneumothorax is another important issue for clinical decisions in the management of pneumothorax...
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Báo cáo y học: " Identification of the 15FRFG domain in HIV-1 Gag p6 essential for Vpr packaging into the virion" pdf
Ngày tải lên :
13/08/2014, 13:20
... packaging Instead, a 15FRFG domain in HIV-1 Gag p6, amino acid residues downstream of the Tsg101-binding domain, is found critical for Vpr virion packaging Since FxFG domains also occurs in nuclear ... reveal if an engineered FRFG domain in these HIV-1 strains can allow Vpr packaging in the absence of the (Lxx)4 domain The Pr-(Lxx)-/R+ construct still maintains a 15FRFG domain in p6 which resembles ... 15FRFG domain in the Gag p6 domain The Vpr packaging function of the 15FRFG domain is preserved when amino It is likely that Vpr recognizes the FxFG domain and not other types of FG repeats Single...
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Báo cáo y học: "Detection of infectious disease outbreaks in twenty-two fragile states, 2000-2010: a systematic review" pps
Ngày tải lên :
13/08/2014, 15:21
... of initial detection of the outbreak were reported; and/or if the time from onset to detection of the outbreak (determined using the definition in Table 1) could be calculated Whenever this information ... Page of 10 Table Definitions used for dates of interest in the outbreak timeline Event Definition Onset For diseases of which one case constitutes a potential outbreak, the date of onset of symptoms ... Borrelia spp relapsing fever in Southern Sudan, 2000 [18]), the mode of detection was unclear, but time to detection was available: these are included in the timeliness findings (see below) Reports...
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Báo cáo sinh học: "Comparison of four statistical methods for detection of a major gene in a progeny test design" pptx
Ngày tải lên :
14/08/2014, 20:20
... interest in new techniques for detection of single genes Various tests have been described concerning livestock (Hanset, 1982) Their general principle is that the within family distribution of ... for m, the number of progeny per sire, the increase of the quantiles is nearly linear with n (number of sires) allowing some extrapolations for higher values of this number - Finally, the jackknife ... Statistical techniques for detection of major genes in animal breeding data Theor Appl Genet 76, 311-319 M6nissier F (1982) Present state of knowledge about the genetic determination of muscular hypertrophy...
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