detection and quantitation not using mass spectrometry

... Lee, and Guodong Chen ț Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu ț Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... TANDEM MASS SPECTROMETRY Tandem MS is the practice of using multiple stages of ion manipulations, including m/z isolation and fragmentation Tandem MS is also referred to as “MS/MS” for two TANDEM ... an ion and a neutral in free space has the center-of -mass (COM) energy ECOM ECOM ¼ mi mn v mi þ mn R ð1:22Þ TANDEM MASS SPECTROMETRY 31 where mi is the ion mass, mn is the neutral mass, and n...

Ngày tải lên: 28/03/2014, 10:20

... extraction 40 2.2.6 Lipid analysis by electrospray ionisation mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) 40 2.2.7 Data processing 42 ... intensity Subsequent targeted analysis using tandem mass spectrometry and collision-induced dissociation allows for quantification of glycerophospholipids and sphingolipids The method was validated ... Analysis and Quantitation by Mass Spectrometry in Methods in Enzymology Submitted 4) Kohler K, Brunner E, Guan XL, Boucke K, Greber UF, Mohanty S, Barth J, Wenk MR and Hafen E A combined proteomic and...

Ngày tải lên: 14/09/2015, 14:06

... Resonance Mass Spectrometry VCH, Wemheim, Germany 15 Busch, K L., Glish, G L., and McLuckey, S A (1988) Mass Spectrometry /Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry ... Biemann, K and Scoble, H A (1987) Characterization by tandem mass spectrometry Science 231,992-998 18 Gtbson, B W and Cohen, P (1990) Ltquid secondary ion mass spectrometry of phosphorylated and sulfated ... (see Note 1) Dissolve the peptide (see Notes and 3) m MES/ACN (30% MES pH 4.0 [see Note 4]/70% ACN [v/v]), Add 2% by volume IAA solution (see Note 5) (100 mg/mL neutrahzed IAA, see Note 6) and...

Ngày tải lên: 11/04/2014, 10:11

... contributions WTS and JW-C prepared the manuscript and developed and performed the PCR assays EA and MC identified and obtained oral cancer tissue EMK and SC-U and identified and obtained cervical ... present and used to derive standard curves in the real time PCR assay for the detection of HPV16 and 18 L1 amplicons Full-length HPV16 genome was PCR-amplified from Caski cell DNA using a Roche Expand ... did not affect the specific amplification and detection of amplicons This assay had a linear range of detection over orders of magnitude, allowing identification/typing of viral genomes and detection...

Ngày tải lên: 12/08/2014, 04:20

... 3) The detection limit of the assay varied for the four serotypes of DENV, the sensitivity of detection was highest for DENV-2 and lowest for DENV-4 (Fig Fig 2C–F, Table 3) Detection and Quantitation ... strains and DENV-4 strains from GenBank using the Clustal W programme A stretch of nucleotides conserved in the four serotypes was identified and the primers and probe sequences were designed using ... primer sets for detection of all four serotypes of DENV [11] In the present study, we describe the development of a DENVspecific TaqMan based real-time PCR for detection and quantitation of all...

Ngày tải lên: 12/08/2014, 04:21

... of: a Number of Cough Candidates that were rejected by the algorithm, and determined as 'not cough' by expert analysis (True Rejected Candidates - TRC) b Number of Cough Candidates that were accepted ... was not designed to detect The overall SENS for detection of cough events for the entire database was 0.96 with SPEC of 0.94 and PPV of 0.90 Table shows the SENS, SPEC, and PPV of cough events detection ... cough events, and the algorithm counted events as well, for the same evaluated minutes Figures and show Bland-Altman plots for correlation between algorithm and expert cough components and events...

Ngày tải lên: 13/08/2014, 10:20

... dimethyl me3 trimethyl MgOAC magnesium acetate MS mass spectrometry MS/MS tandem mass spectrometry MudPIT multi-dimensional protein identifiation n/a not applicable NaCl sodium chloride NDE no dynamic ... HTA1 and HTA2 (histone H2A), HTB1 and HTB2 (histone H2B), HHT1 and HHT2 (histone H3), and HHF1 and HHF2 (histone H4) The two genes for histone H2A have extremely high sequence identity and the ... through the confusing and frustrating times Thank you for caring about me and keeping me fed • Melanie Fox, for answering my questions and keeping my spirits up when my experiments did not work out...

Ngày tải lên: 24/08/2014, 11:58

... contribution of Met1, and Met23 substituted for Leu, Fig Comparison of the cyclization reaction as a function of temperature and pH (A), (B) and (C): (d), (s) and (.) correspond to pH 2.5, 8.0 and 11.5, ... hydrolysis reaction as a function of denaturing agent and pH (A), (B) and (C): (d), (s) and (.) correspond to guanidinium chloride concentrations of , and M, respectively Hydrolysis was calculated as ... (M23L) signal in the MALDI-TOF mass spectra h after the addition of AAP The pH was adjusted to 2.5, 8.0 and 11.5 and the samples were incubated at 37 °C, 50 °C and 70 °C Aliquots were taken at...

Ngày tải lên: 19/02/2014, 12:20

... interactions using soft ionization mass spectrometry Int J Mass Spectrom 216, 127 54 Heck AJR & van den Heuvel RHH (2004) Investigation of intact protein complexes by mass spectrometry Mass Spectrom ... cyclotron resonance mass spectrometry: a primer Mass Spectrom Rev 17, 135 56 Smith RD (2000) Evolution of ESI -mass spectrometry and Fourier transform ion cyclotron resonance for proteomics and other biological ... TRX II and CTX using high-eld ESI FT-ICR MS and X-ray crystallography Results and Discussion ESI FT-ICR analyses Figure presents typical 9.4 T ESI FT-ICR mass spectra of TRX I, TRX II and CTX...

Ngày tải lên: 07/03/2014, 17:20

... observable from the mass isotopomer distribution of lysine, fragments [1-4] and [2-4] of OAA and [1-3] and [2-3] of PYR [7], the obtained measurements of these mass isotopomer fractions are not adequate ... carbons and and to a lesser extent of and of lysine that enables the estimation of the flux ratio at H4D using NMR measurements Finally, the irreversibility between the PEP and the PYR and OAA ... labeling used and the value of the unknown fluxes), but the opposite does not necessarily hold true It cannot identify numerical Ó FEBS 2003 Systematic flux analysis using stable isotopes and MS (Eur...

Ngày tải lên: 08/03/2014, 08:20

... 13C- and 12C1H- (mass 13.003355 and mass 13.007825, respectively; Figure 15b); 12C14N- and 1H12C13C (mass 26.003074 and mass 26.011180, respectively; Figure 15c); 12C15N-, 13C14N- and 12C14N1H- (mass ... could using a stationary beam, and also to associate quantitation and localization among cells and subcellular domains All the mass imaging in the rest of this paper will refer to quantitative mass ... By contrast, E faecalis is visible at mass 12C14N(Figure 5a) but not at mass 12C15N- (Figure 5b) because it does not use gaseous nitrogen and therefore has not incorporated 15N above the natural...

Ngày tải lên: 06/08/2014, 18:21

... gel electrophoresis (SDS-PAGE) and peptide mass sequencing using liquid chromatography–tandem mass spectrometry (LC–MS/MS) Results and Discussion • Preparation and characterization of ethyleneglycol-protected ... microbalance [16], mass spectrometry [17–19], and microarrays [20–26] With the power of peptide sequencing and database searches for unknown protein identification, however, mass spectrometry has ... visualization and peptide sequence identification using liquid chromatography–tandem mass spectrometry (LC–MS/MS) Conclusions: The assay combining efficient magnetic separation and MALDI–MS readout...

Ngày tải lên: 11/08/2014, 08:20

... by exact mass measurement and coelution with authentic standards Data processing The raw mass spectrometry data of all samples were processed (deconvolution, alignment, deisotoping and data reduction) ... inferred from mass spectrometry intensities Besides the intensity profile vector xi, also a particular retention time (rt) index and mass- to-charge ratio (m/z) is associated with each marker candidate ... marker candidates (prototype 1) over time stable candidates (prototypes 5–7) towards very early enhanced and transient marker candidates (prototype 9) can be observed Thus, block A represents candidates...

Ngày tải lên: 12/08/2014, 17:20

... and identified using mass spectrometry and database searching [4] 2-DE together with MS has previously been used to investigate the protein content in nasal lavage fluid (NLF) [5] and a study of ... Pteronyssimus and D Farinae), molds (Cladosporium and Alternaria) and animal allergens (cat, dog and horse) SPT were performed on the volar side of the forearm with saline buffer as negative and histamine ... search, western blot and drafted the manuscript MA and LOC conceived the study, participated in its design and coordination and helped to draft the manuscript Page of (page number not for citation...

Ngày tải lên: 12/08/2014, 18:22

... cytidine, and thymine [10,11,17,18]; maleic acid and succinic acid derivatives [6,19,20]; benzenoids [21]; phenol and tocopherols [8,22]; 5’-nucleotides [14]; and diterpenes [23] No chemical standardization ... coupled with diode array detector and mass spectrometry (HPLCDAD-ESI-MS) and to assess the quality of two commercial mycelial AC products Methods Plant Powdered mycelium and an intact fruiting body ... times and UV absorption profiles with respect to standards and was confirmed using ESI-MS In total, ten compounds were identified in the water extract of mycelia AC However, adenine, cytosine, and...

Ngày tải lên: 13/08/2014, 15:21

... spectra data generated by mass spectrometry (MS) or tandem mass spectrometry (MS/MS) The advance in tandem mass spectrometry (MS/MS) technology has made highthroughput mass spectra generation possible ... peptide and PTM identification using tandem mass spectrometry The main concern for algorithms in peptide identification is achieving fast and accurate peptide identification by mass spectrometry ... problem of peptide identification by tandem mass spectrometry, the input includes the mass spectrum S, the set of possible ion types ∆ and the parent mass M (and for database search algorithms,...

Ngày tải lên: 12/09/2015, 08:18

... protein and as deuterium is one mass unit heavier than hydrogen so upon deuteration the protein also becomes correspondingly heavy and this change in mass can be monitored by high resolution mass ... monomeric and contains a single cAMP binding site making it a facile model to understand detailed and structure and dynamics of intersubunit interactions in PKA by both X-ray crystallography (27) and ... Ala97, Ile98 and Ser99), the linker region (Ala100, Val102, Val103, and Glu105), the αXn-αA loop (Val134, Leu135, and His138), αA (Glu143), αB (Glu200, Leu201, Leu204 and Tyr205), and αC (Arg230,...

Ngày tải lên: 16/10/2015, 15:36

... provides an instant response and does not require sample pre-treatment, making it a powerful and convenient tool for the on-line characterization and full control of chemical and pharmaceutical reactions ... reactions using EESI-MS Figure Mass spectra of the Michael addition reaction recorded at 20, 60 and 300 min, respectively Inserts: molecular structures of PEA, PEAP and CPEAP 2995 (PAs) and remain ... In addition to real-time monitoring, tandem mass spectrometry (MSn) helps to identify unknown species, validate proposed intermediates and further understand the reaction mechanisms To demonstrate...

Ngày tải lên: 14/02/2014, 08:20

... patients confidence and quantitation accuracy for proteomic research, especially for low-abundance proteins [16,17] We used iTRAQ labeling together with gel-assisted digestion and mass spectrometry ... result Using the iTRAQ labeling mass spectrometry, a total of 438 proteins were identified by our proteomic platform To better understand the roles of these identified proteins, they were grouped and ... expression was compared between each tumor and adjacent normal tissue using LC-MS ⁄ MS analysis (Fig 1) In our previous study using the same proteomic platform, quantitation of four independently purified...

Ngày tải lên: 16/02/2014, 15:20

... Conformational studies on the selectin and natural killer cell receptor ligands sulfo- and sialyl-lacto-N-fucopentaoses (SuLNFPII and SLNFPII) using NMR spectroscopy and molecular dynamics simulations ... pattern, sequence, and partial linkages, and assign structural details of each branch (e.g the 3- and 6-branches) Blood group H, Lea/Lex and Leb/Ley determinants, together with type and type chains, ... pattern and sequence analysis of underivatized oligosaccharides by combined MS/MS of singly and doubly charged molecular ions in negative-ion electrospray mass spectrometry J Am Soc Mass Spectrom...

Ngày tải lên: 19/02/2014, 12:20