detection and analysis of rna viruses

... more intricate, and thus more easily influenced folding of the strands (Highsmith, 1999) Detection and analysis of genetic alterations in normal skin and skin tumours The detection of fragments ... follicle Sebaceous gland Sweat gland Fat Figure Histological section of normal epidermis (A) and a schematic picture of the different layers of the skin (B) 25 Detection and analysis of genetic alterations ... Principles of methods used for detection of mutations/variations at defined positions 15 Detection and analysis of genetic alterations in normal skin and skin tumours Pyrosequencing The principle of...

Ngày tải lên: 10/04/2014, 22:12

... samples collected over a period of six years from India Preparation of RNA standard The detection limit of this assay was determined using RNA standards The RNA standard was produced using T7 transcription ... led to successful isolation of Dengue type 2, and viruses (Table 1) RNA was extracted from culture supernatant and used as template in RT-PCR and nested PCR The RT-PCR and nested PCR revealed desired ... Isolation of Singh's Aedes albopictus cell clone sensitive to dengue and chikungunya viruses J Gen Virol 1978, 40:581-4 Gould EA, Clegg TCS: Growth, assay and purification of Alphaviruses and Flaviviruses...

Ngày tải lên: 20/06/2014, 01:20

... the combination of splice sites present in the premRNA, and may occur through four basic modes: use of an alternative 5'ss, use of an alternative 3'ss, cassette-exon skipping and intron retention ... 8,614 and 8,172 clones in the expo, stat and oleic libraries, respectively About 59% of the predicted genes (3,818 of 6,449) were expressed and found in at least one library and about 70% of these ... both alternative transcripts, exon was skipped either totally (splicing between 5'ss of intron and 3'ss of intron 3) or partially (alternative 3'ss of intron 2, leaving 45 nucleotides of exon...

Ngày tải lên: 09/08/2014, 20:22

... 2006, Andhra Pradesh, India Methods Viruses Sixteen strains of dengue viruses, including five strains of DENV-1, four strains of DENV-2, three strains of DENV-3, four strains of DENV-4 and one ... directly for sequencing and for evaluation of sensitivity of the real-time PCR JE, WN and CHK viruses were used to evaluate the specificity of the assay Infection and maintenance of cell cultures Vero ... specimens/(number of positive specimens + number of false negative specimens)] The specificity of the assay was evaluated using RNA extracted from virus stocks of two related Flaviviruses (JE and WN), and...

Ngày tải lên: 12/08/2014, 04:21

... appendix of Additional data file that this is indeed the case for these six genomes Random SNDs can and form clusters of identical patterns simply by chance Given the number of SNDs and their ... locally collinear block Concatenation of LCBs results in a G × M matrix of nucleotides and gap symbols, where G is the number of genomes and M is the length of gapped alignments across all blocks ... Seillier-Moiseiwitsch F: Stepwise detection of recombination breakpoints in sequence alignments Bioinformatics 2005, 21:589-595 Stephens JC: Statistical methods of DNA sequence analysis: detection of intragenic...

Ngày tải lên: 14/08/2014, 16:21

Ngày tải lên: 29/11/2014, 06:59

... H3N2 viruses 6.3.1.1 HA and NA diversity 6.3.1.2 Diversity of internal genes 6.3.2 Seasonal H1N1 viruses 6.3.2.1 HA and NA diversity 6.3.2.2 Diversity of internal genes 6.3.3 Pandemic H1N1/09 viruses ... for detection of Influenza A and B viruses 2.2.3.3 Five-plex Real-Time TaqMan PCR for influenza A and B virus detection 2.2.3.4 Multiplex RT-PCR protocol for the detection of Adenovirus and Bocavirus ... Sensitivity of molecular and viral isolation methods for detection of influenza A virus infection during the period of surveillance and the methods employed 77 Table 5.3 Comparison of sensitivity of conventional...

Ngày tải lên: 09/09/2015, 08:18

... °C in standard buffer: 50 mM Tris, 150 mM NaCl, pH 7.5 MS2 bacteriophage and mutant samples Fig Structure of the whole capsid of bacteriophage MS2 and of the coat protein dimer bound to RNA (A) ... emitted at wavenumber mi and the summation is carried out over the range of appreciable values of F The values of center of spectral mass of tryptophan were converted into degree of denaturation/dissociation ... fraction Fig Dissociation and denaturation of wild-type (WT) and mutant MS2 particles (A) Light scattering and spectral center of mass measurements of WT MS2 as a function of urea concentration To...

Ngày tải lên: 19/02/2014, 12:20

... TMV RNA RNA RNA RNA RNA RNA RNA 203–674 of TMV RNA 483–504 390–409 420–440 390–674 549–569 Non-TMV 84 0 89 89 70 330 5· 5· 4.5 · 330 4.5 · 330 siRNA siDNA 21 bp 21 bp HIV1gp41 RNA 257–277 of gp41 ... (Fig 2) Application of higher concentrations of siDNAs than of siRNAs is necessitated by the lower stability of RNA DNA than of RNA RNA duplexes formed within RISC On the other hand, DNA oligomers ... the antiviral effects of short and long DNA and RNA molecules specific for TMV genomic RNA We have found that both long dsRNA and long dsDNA are potent inhibitors of viral RNA A long dsDNA fragment...

Ngày tải lên: 16/03/2014, 02:20

... the position of the plus-strand end and the y-axis gives the position of the minus-strand end The colors and sizes of the points indicate the number of reads aligning to each pair of coordinates ... effective total number of reads n1 and n2 in a pair of samples (or pair of groups of samples), we estimate q2, which is the fraction of reads in the second sample, and then set n = q N and n = N - n where ... ExpressionPlot offers the gene expression community an easy-to-use tool for automated analysis of gene expression and RNA processing data The back end offers a solution to the problem of detecting...

Ngày tải lên: 09/08/2014, 23:20

... were aware of in the vicinity of the Saltol locus in FL478 originated from the salt sensitive parent Results and discussion SFP detection and validation By applying higher density SFP analysis ... spanned one SNP and one INDEL, one (1.6%) spanned > SNP and no INDEL, and one spanned > SNP and > INDEL From this we assert that at the threshold of top 20 percentile outlying scores, our detection ... for expression analysis, provides a satisfactory genetic marker system for mapping in rice using RNA hybridization and the RPP method of SFP analysis Methods Plant materials Seeds of rice (Oryza...

Ngày tải lên: 12/08/2014, 03:20

... [14] Statistical Office of the Republic of Serbia National Accounts of Serbia, Gross Domestic Product of the Republic of Serbia (1997-2007) Statistical Office of the Republik of Serbia, 2009 [15] ... the fields of energy policy and modelling, development & administration of DSS and in lectures and seminars of the NTUA and the Technical Chamber of Greece regarding energy policy and DSS He ... for RES, Ministry of Mining and Energy of the Republic of Serbia, whose helpful remarks and fruitful observations were invaluable for the development of this work The content of the paper is the...

Ngày tải lên: 05/09/2013, 16:10

... prohibited 90 Lee and off-line interview and test tools, and then the company conducts an online pre-employment background check and makes a job offer to the best candidate Categories of E-Recruiting ... Wall Street Journal, focuses exclusively on the career needs of executives, managers, and professionals, leveraging the Wall Street Journal brand CareerJournal.com provides recruiters and job seekers ... identification of hiring needs; submission of job requisition and approval; job posting, submission of job applications; screening of résumé/application; interviewing; pre-employment screening; and job offer...

Ngày tải lên: 24/10/2013, 08:20

... SCHWARZ - SOUND and TV BROADCASTING – CCIR and FFC tv standards – Printed in the federal republic of Germany 18 3GPP2 C.S0011-C, version 2.0 – Recommended Minimum Performance Standards for cdma2000 ... coding and modulation for cable systems – European Broadcasting Union 13 ETSI EN 302 307 V1.2.1 (2009-08) – Digital Video Broadcasting (DVB); Second generation framing structure, channel coding and ... channel coding and modulation systems for Broadcasting, Interactive Services, News Gathering and other broadband satellite applications (DVB – S2) 14 Tim Williams – EMC for Product Designers – Newnes...

Ngày tải lên: 26/11/2013, 20:44

Ngày tải lên: 14/12/2013, 12:15

... from CONICET and ´ ´ Agencia Nacional de Promocion Cientı´ fica y Tecnologica M F Drincovich and C S Andreo are members of the Researcher Career of CONICET, and V A Campos-Bermudez and F P Bologna ... phosphotransacetylase Results Expression and purification of E coli Pta and truncated Ptas containing the C-terminal end By analysis of the protein domain architecture of E coli Pta, three conserved domains ... obtained for Pta-F2 and Pta-F1 (B) Activation of the acetyl phosphate synthesis activity of E coli Pta by different concentrations of pyruvate and PEP Results are presented as percentage of activity...

Ngày tải lên: 06/03/2014, 11:20

... FOR CORPORATE ETHICS AND GOVERNANCE The Center for Corporate Ethics and Governance is committed to improving public understanding of corporate ethics, law and governance, and to identifying specific ... of 2008 has aroused the interest of U.S policymakers in the mechanisms of corporate governance, compliance, and ethics, and their collective role in preventing and mitigating excesses and scandals ... Sarbanes-Oxley reforms of 2002 responded to a stream of corporate accounting and fraud scandals by mandating new ethics hotlines, codes of conduct, and stronger internal controls and reporting efforts,...

Ngày tải lên: 06/03/2014, 22:20

... helices AV and BV at the surface of domain V Gly621 and Gly617 are in the area of contact with the 1095 and 2473 regions of 23S RNA The two helices are facing the ribosome, and the four-stranded b-sheet ... binding and ˚ located in the intersubunit space at a distance of 10 A ˚ from residue 2655 of 23S RNA, 15 A from ribosomal ˚ protein L14, and 10 A from residue 342 of 16S RNA The switch II region of ... type and various mutants, domains III, IV and V display a movement relative to domains I and II, resulting in a shift of the ˚ tip of domain IV of up to A [14,16] The ribosomebound structure of...

Ngày tải lên: 06/03/2014, 22:21

... Automatic Correction of Errors In this section we propose our algorithm for automatic correction of errors, which consists out of the following steps: Automatic detection of error candidates, i.e cases ... results different to gold-standard Substitution of the annotation of the error candidates by the annotation proposed by one of the parsers (in our case MSTParser) Parse of the modified corpus with ... detection and correction of errors and compared it to the only other work we have found in this field Our results show that both approaches are rather complementary and find different types of...

Ngày tải lên: 07/03/2014, 22:20

... analysis of metabolites Reactions were initiated by the addition of 100 ll of a solution of enzyme (200 lgÆmL)1) to 0.9 mL of a solution of 50 lg of substrate in 10% (v/v) dimethyl sulfoxide and incubated ... measurement of activity is described above To examine the enzymatic incorporation of 18O2 and 18O-labeled water into GG, reactions were initiated by the addition of 20 lL of a Enzymic reaction and analysis ... 0.1 mL of EC or CY to 1.0 mL of VM containing 10 lg of GOU For HP and M, reactions were initiated by addition of 0.1 g of HP or M to 1.0 mL of VM containing 10 lg of GOU After incubation for 12...

Ngày tải lên: 17/03/2014, 03:20