desensitization of purinoceptor mediated signaling
Báo cáo y học: "Small GTP-binding protein Rho-mediated signaling promotes proliferation of rheumatoid synovial fibroblast" pot
Ngày tải lên :
09/08/2014, 06:22
... important pathological role in RA synovitis The aim of the present study was to determine the role of Rho -mediated signaling in the regulation of synovial proliferation and cytokine production ... green-fluorescent protein (GFP), the C-terminal regions of Gα12 (Gα12-ct), the C-terminal regions of Gα13 (Gα13-ct), and the regulator of the G-protein signaling domain of p115RhoGEF (p115RGS) were produced as ... the responses of RA SFs were significantly higher than those of OA SFs after 24 hours of incubation Involvement of small GTP-binding protein Rho activation in thrombin-induced signaling in RA...
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Báo cáo y học: "Knockdown of Rab5a expression decreases cancer cell motility and invasion through integrin-mediated signaling pathway" ppsx
Ngày tải lên :
10/08/2014, 10:20
... integrinmediated signaling pathway and the exact roles of Rab5a in cancer progression remain unclear In the present study, we investigated the mechanism of Rab5a involving in the progression of cervical ... enhance the integrin -mediated signaling pathway, and induce the cancer cell migration and invasion Page of Figure Rab5a regulates the activity of Rho GTPases and reorganization of actin cytoskeleton ... and membrane of cancer cells Qualitation of the staining was determined by the percentage of cells showing positive immunoreactivity (0, no staining; 1, 50% of cells),...
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Báo cáo y học: " Real time analysis of b2-adrenoceptor-mediated signaling kinetics in Human Primary Airway Smooth Muscle Cells reveals both ligand and dose dependent differences" ppt
Ngày tải lên :
12/08/2014, 13:22
... change of the probe due to the binding of cyclic AMP was analysed in terms of (1) time lag between the direct addition of drug into the buffer surrounding the cell and the initiation of a measurable ... binding of cyclic AMP to Epac and a subsequent conformational change of Epac leading to an increased distance between the CFP and YFP termini of the probe and a subsequent loss of FRET The trace of ... profile of a range of b2-adrenoceptor agonists One major advantage of the described Epac-based studies utilising the spinning disk imaging system is the ability to measure b -adrenoceptor-mediated...
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Mechanism of hydrogen sulphide mediated signaling cascade through n methyl d aspartate receptors
Ngày tải lên :
10/11/2015, 12:35
... supervisors, Dr Steve Cheung Nam Sang of Department of Biochemistry, NUS, Prof Philip Moore of Department of Pharmacology, NUS and, Dr Deng Lih Wen of Department of Biochemistry, NUS for their invaluable ... and guidance Mr Jayapal Manikandan of Department of Physiology and Prof Maxey Chung, and Miss Tan Gek San of Department of Biochemistry had rendered in my course of research, that had truly benefited ... in H2S- 121 mediated neuronal death 4.2 Proposed signaling cascade of H2S -mediated signaling cascade 122 through NMDA receptor 4.3 Similarities and differences between H2S- and NMDA -mediated 126...
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Tài liệu Báo cáo khoa học: Compartmentalization and in vivo insulin-induced translocation of the insulin-signaling inhibitor Grb14 in rat liver pptx
Ngày tải lên :
18/02/2014, 18:20
... inhibits the tyrosine kinase activity of the IR [7] On the basis of the crystal structure of the tyrosine kinase domain of the IR in complex with the PIR ⁄ BPS domain of Grb14, Depetris et al [8] have ... groove of the kinase and thus acts as a selective inhibitor of insulin signaling Consistent with this observation, overexpression of Grb14 in CHO-IR cells impairs Akt and ERK insulin signaling ... from livers of rats studied at the indicated time points after injection of 30 lg of insulin (four to six rats per time point) (A), or studied after injection of the indicated dose of insulin...
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Tài liệu Báo cáo khoa học: Role of receptor-mediated endocytosis, endosomal acidification and cathepsin D in cholera toxin cytotoxicity pdf
Ngày tải lên :
19/02/2014, 02:20
... detected as a doublet of 47 kDa and 45 kDa (Fig 1, lanes of lower blots) In vivo injection of native CT or CT-B effected a rapid increase of both the 47 kDa and 45 kDa Gsa isoforms, with maximal ... acidification at the early stage of CT action A B Role of endosomal acidification and cathepsin D in CT action Fig CT -mediated recruitment of ARF-6 and ADP-ribosylation of Gsa in hepatic endosomes ... endosomal locus after a lag phase of 30 Third, CT -mediated hyperacidifi- cation of endosomes increased over a time course similar to that of endosomal activation of CT Therefore, we propose that...
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Báo cáo khoa học: Noninvasive imaging of microRNA124a-mediated repression of the chromosome 14 ORF 24 gene during neurogenesis potx
Ngày tải lên :
07/03/2014, 02:20
... of miR124a and the 3¢-UTR of the c14orf24 gene showed a strong evidence of miR124amediated repression of c14orf24 during neurogenesis The coding region of c14orf24 (NM_173607), the function of ... activity of CMV ⁄ Gluc ⁄ c14ofr243¢UTR slightly decreased to the third day of neuronal differentiation of P19 cells (Fig 4D) The significant decrease in Gluc activity from CMV ⁄ Gluc ⁄ c14ofr243¢UTR ... increase of miR124a expression during the neuronal differentiation of P19 cells treated with RA, the endogenous gene expression of five of the candidates was gradually decreased over the period of neuronal...
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Báo cáo khoa học: Protein kinase Ce: the mitochondria-mediated signaling pathway pot
Ngày tải lên :
23/03/2014, 07:20
... involvement of PKCe The outline of two main apoptosis pathways (TRAIL -mediated and mitochondria -mediated) is shown The TRAIL -mediated apoptosis pathway is triggered by activation of TRAIL receptors ... proteolytic activation of Bid by caspase-8 (formation of tBid) fi binding of tBid to MOM fi activation of BAX and BAK to translocate from cytoplasm to MOM fi destabilizing of MOM fi release of cytochrome ... etc.) fi translocation of NOXA, PUMA fi inhibition of anti-apoptotic protein Bcl2 fi destabilization of mitochondrial membrane potential fi release of cytochrome c fi activation of Apaf-1, and then...
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Báo cáo khoa học: Tec family kinases: regulation of FceRI-mediated mast-cell activation ppt
Ngày tải lên :
28/03/2014, 22:21
... leading to Ca2+ mobilization and the activation of protein kinase (PK)C isoforms Formation of the LAT signaling complex leads also to activation of the mitogen-activated protein kinase (MAPK) ... activation of the Src family kinase Lyn, which activates Syk, a member of the Syk ⁄ ZAP-70 kinase family This results in the phosphorylation of several tyrosine residues in LAT and the formation of a signaling ... activation of PKC isoforms FceRI cross-linking leads also to the activation, via Grb2 ⁄ son of sevenless (SOS) of the MAPK Erk1 ⁄ 2, p38 and JNK1 ⁄ Together, the signals lead to the activation of transcription...
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Báo cáo khoa học: Homologous desensitization of guanylyl cyclase A, the receptor for atrial natriuretic peptide, is associated with a complex phosphorylation pattern pot
Ngày tải lên :
29/03/2014, 09:20
... phosphorylation of this residue in the inhibitory regulation of GC-A activity To follow this hypothesis, we tested the influence of the substitution of Ser487 with glutamate on the process of homologous desensitization ... is phosphorylated during homologous desensitization Hence, homologous desensitization of GC-A is associated with a complex pattern of (de)phosphorylation of the receptor, with a selective prominent ... dephosphorylation of the previously postulated sites (Ser497, Thr500, Ser502, Ser506, Ser510 and Thr513), homologous desensitization of GC-A involves the phosphorylation of Ser487 Indeed, the results of site-directed...
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Báo cáo khoa học: Membrane compartments and purinergic signalling: the role of plasma membrane microdomains in the modulation of P2XR-mediated signalling pot
Ngày tải lên :
30/03/2014, 02:20
... is very often the first step in the biochemical analysis of the role of these domains in cellular signalling After disruption of cells, lipid rafts ⁄ caveolae can be extracted by virtue of their ... observed upon lipid raft disruption Blockade of receptor -mediated currents and artery contraction – ND ND – – Inhibition of receptor -mediated activation of PLA2 and SMase Ref [83,88] [87] [89] [21,89–92] ... description of the tissue distribution and function of the P2X receptor in a subtype-specific way is given in more comprehensive reviews [10,11] The (physio)pathological implications of this subfamily of...
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Báo cáo khoa học: Identification of intracellular target proteins of the calcium-signaling protein S100A12 pdf
Ngày tải lên :
30/03/2014, 15:20
... recognition of annexin I [22] Thus, there are a number of modes of interaction between members of the annexin family and those of the S100 family Although the precise physiological significance of these ... Ca2+-dependent [44] The mode of interaction of annexin V with S100A12 differs from the mode of annexin II– S100A10 interaction In the case of the latter, the C-terminal domain of S100A10 was proven ... recognition of annexin [45] However, in the case of the annexin XI–S100A6 interaction, helix I of S100A6 was found to be essential for the recognition of annexin XI [46] Similarly, helix I of S100A11...
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Báo cáo hóa học: "Rac1-mediated signaling plays a central role in secretion-dependent platelet aggregation in human blood stimulated by atherosclerotic plaque" ppt
Ngày tải lên :
18/06/2014, 16:20
... perfused surface fields of the size of 237900 μm2 (located in the middle of the channels of the viewing window) were recorded, and fluorescence images were later analyzed off-stage by quantifying ... important observation of our study concerns the role of integrin a IIb b outside-in signaling in the regulation of ATP-secretion in stirred activated blood RGDS reduced ATP-secretion of stirred blood ... University of Munich and the Bayern University ("BayEFG"; to A.L.K.) The results are part of the doctoral thesis of S.D at the University of Munich Author details Institute for Prevention of Cardiovascular...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
Ngày tải lên :
20/06/2014, 07:20
... repression activity of the Mad-SID is mediated through interactions of the Mad N-terminus with the mammalian Sin3 co-repressor protein [10,11] The effectiveness of the Sin3 binding domain of Mad in silencing ... evidence of ovarian expression of these genes A total of 71 of the genes were found to be expressed in the ovary and have been categorized according to function: Gene Regulation (Table 1), Signaling ... activity of the GnRHR promoter was determined by comparing the luciferase activity of the promoter in the presence of pFoxl2 (over-expression) to the activity of the promoter in the absence of Foxl2...
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Báo cáo sinh học: "Shrinkage control: regulation of insulin-mediated growth by FOXO transcription factors" ppt
Ngày tải lên :
06/08/2014, 18:20
... comprised of fewer cells of normal size [4] Thus, changes in cell size and cell number are genetically separable outcomes of insulin signaling, and dFOXO represents the first identified insulin signaling ... effects of alterations in insulin levels Together, these new studies in Drosophila significantly broaden our understanding of the multiple layers of insulin -mediated growth regulation Control of gene ... promote cell growth through a number of effects including stimulation of ribosome biogenesis [7] The direct target of Akt in this case appears to be the product of the tuberous sclerosis complex...
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Báo cáo khoa học: "The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain" doc
Ngày tải lên :
07/08/2014, 20:23
... insertion mutagenesis of the 88 MDa plasmid, mediated by the TnphoA transposon, probably impaired the function of the genes essential for mediation or regulation of the expression of such characteristics ... sterilized saline solution) of the strain to be tested -1 -11 was diluted ten-fold (10 to 10 ) and 0.5 ml of each dilution was injected subcutaneously into the neck region of groups of six one-day-old-male ... detection of adhesin-related genes of avian Escherichia coli strains Vet Microbiol 2005, 106, 275-285 Carbonetti NH, Williams PH A cluster of five genes specifying the aerobactin iron uptake system of...
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Báo cáo y học: "Adverse effects of adenovirus-mediated gene transfer of human transforming growth factor beta 1 into rabbit knees" potx
Ngày tải lên :
09/08/2014, 01:21
... Ad.TGF-β1-treated joints, there was evi- dence of both fibroblast and myofibroblast proliferation between myofibers with intracellular edema, but there was no evidence of inflammation or myonecrosis (Fig ... gene transfer of TGF-β1 could be therapeutic for the treatment of rheumatoid arthritis as well as osteoarthritis To examine the effects of TGF-β1 on joint pathology, we used adenovirus -mediated intra-articular ... appropriate for the enhancement of repair of cartilage defects Instead, for TGF-β1 gene therapy to be effective in promoting repair of damaged cartilage, the level of TGF-β1 will need to be highly...
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Báo cáo y học: "γ Paradoxical roles of IFN-γ in models of Th1-mediated autoimmunity" potx
Ngày tải lên :
09/08/2014, 06:22
... original paradigm of Th1 and Th2 cell function described a distinct separation of cytokines and function in T-cell-dependent immune responses, through the use of a number of models of autoimmunity ... complexities of the biological immune responses These complexities are perhaps best demonstrated in the analysis of the role of IFN-γ in models of autoimmunity Early attempts to define the role of IFN-γ ... levels of IL-1β in the lymph nodes and synovial cells of arthritic B6 IFN-γ–/– mice The elevated levels of IL-1β appear to be important for the development of the disease, as treatment of B6 IFN-γ–/–...
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Báo cáo y học: "The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis" potx
Ngày tải lên :
09/08/2014, 07:20
... models, expression of the CTR occurs during the terminal stage of osteoclast differentiation, and activation coincides with the competence of the cell to resorb bone The expression of this gene and ... area of extensive periimplant osteolysis along the femoral shaft Available online http://arthritis-research.com/content/8/3/R70 Specimens of soft tissue and bone were collected from regions of ... zones of osteolysis develop as a consequence of an active biologic process involving the resorption of bone at the peri-implant sites Insights into the mechanisms involved in this focal disorder of...
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Báo cáo y học: "PLCb1-SHP-2 complex, PLCb1 tyrosine dephosphorylation and SHP-2 phosphatase activity: a new part of Angiotensin II signaling?" ppt
Ngày tải lên :
10/08/2014, 05:21
... effects of losartan, an Ang II type receptor blocker, as well as by altering, via its silencing, the levels of the regulator of G protein signaling (RGS-2), a key control element of Ang II signaling ... Department of Clinical and Experimental Medicine, Clinica Medica University of Padova, School of Medicine, Italy 2Department of Biological Chemistry, University of Padova, School of Medicine, ... dephosphorylation of PLCb1 and the phosphorylation of SHP-2 The effect of Ang II on these was further demonstrated by the blocking of these changes found by incubation in the presence of losartan Moreover...
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