... models or in the surroundings of the mutated residues. The models of RNase A covalent dimers in the exchanging conformation were prepared using the 7RSA structure and the structure of exchanging BS-RNase (1BSR) ... through the exchange between subunits of either their N-ter- minal, or their C-terminal ends [33–35]. We found that the behavior with the model membrane of the dimer in which the C-terminal ends ... were prepared using models of these dimers. The models of RNase A covalent dimers in the nonexchanging conforma- tion were prepared using the 7RSA structure and the struc- ture of nonexchanging BS-RNase...
Ngày tải lên: 19/02/2014, 06:20
Báo cáo khóa học: Mutational and computational analysis of the role of conserved residues in the active site of a family 18 chitinase docx
... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme. To obtain an insight into ... by the surrounding residues. The results so far indicate that larger parts of the polar core of the catalytic TIM barrel of family 18 chitinases play a role during catalysis. It will thus be interesting ... conformations. The Asp142 equiv- alent in the family 18 chitinase from Coccidioides immitis was found to be in the ÔdownÕ position in the apoenzyme and in the ÔupÕ position in the complex with allosamidin...
Ngày tải lên: 07/03/2014, 14:20
Báo cáo Y học: Role of tyrosine 238 in the active site of Rhodotorula gracilis D-amino acid oxidase A site-directed mutagenesis study docx
Ngày tải lên: 17/03/2014, 17:20
Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx
Ngày tải lên: 09/08/2014, 06:22
Tài liệu University Oars Being a Critical Enquiry Into the After Health of the Men Who Rowed in the Oxford and Cambridge Boat-Race, from the Year 1829 to 1869, Based on the Personal Experience of the Rowers Themselves pdf
... when living in damp air). If the induration of the right lung which was observed in A.'s case really commenced at the time he experienced pain in rowing in the College races, then possibly the ... participated in the struggle—nine of the cases resting on the evi- dence of men who themselves rowed and are still alive, and the remaining eight on reports received from some of the relatives of those ... have arisen originally from the bursting of a blood-vessel through his exertions in rowing, either in the practice for the Inter-University Race or in the race itself." Some of these contemporaries...
Ngày tải lên: 14/02/2014, 21:20
Báo cáo y học: " Cost effectiveness of antimicrobial catheters in the intensive care unit: addressing uncertainty in the decision" doc
Ngày tải lên: 13/08/2014, 16:20
Globalization: the Role of Institution Building in the Financial Sector _ The Case Study of China
... under the framework of financial globalization. II. Review of Institution Building in the Financial Sector A. Main driving forces of institution building A review of the developments of China’s financial ... performance. 2. Role of the central bank The central bank has played a role of policy designing and implementation in the process of institution building in the financial sector. The promulgation of The ... source of financing for enterprises, if their own capital is excluded. In summary, the deepening process of the financial markets has rendered the dominance of the banking sector in the financial...
Ngày tải lên: 18/10/2013, 07:15
Tài liệu The Role of BCG Vaccine in the Prevention and Control of Tuberculosis in the United States: A Joint Statement by the Advisory Council for the Elimination of Tuberculosis and the Advisory Committee on Immunization Practices docx
... ( 39 ). The renewed interest in examining the indications for BCG vaccination in the United States included consideration of the wide range of vaccine efficacies deter- mined by clinical trials ... to explore the sources of the heterogeneity in the efficacy of the BCG vaccine reported in the individual studies. Using a model that included the geographic latitude of the study site and the data ... <5 mm induration after skin testing with 5 TU of PPD tuberculin. The Tice strain of BCG is administered percutaneously; 0.3 mL of the reconstituted vaccine is usually placed on the skin in the lower...
Ngày tải lên: 15/02/2014, 13:20
Tài liệu The Role of Community Banks in the U.S. Economy pdf
... since the mid-1990s says more about the local economies in which they operate than the viability of community banking as a business model. Implications of advances in information-gathering technology ... an important role in the financial system of the U.S. economy. They complement the role of large banks by special- izing in relationship banking and providing credit to small businesses—a sector ... “Small Business Lending and the Changing Structure of the Banking Industry,” Journal of Banking and Finance, August. Sullivan, Richard J. 2000. “How Has the Adoption of Internet Banking Affected Performance...
Ngày tải lên: 16/02/2014, 11:20
Tài liệu SICK WATER? THE CENTRAL ROLE OF WASTEWATER MANAGEMENT IN SUSTAINABLE DEVELOPMENT pdf
... cost-effective way of as- sessing health risks; the identification of all risk points along the chain of events from the origin of the wastewater to the consumption of the produce (e.g. the farm-to-fork ... Ho- epner,2008). There is scope to improve the sustainability of the desalination process. The process results in the discharge of a concentrated brine into the receiving waters. Temperature and salinity ... approach of the HAPPC meth- od in food safety); the design of a combination of health risk management mea- sures, to be applied along the same chain of events, with the aim of ensuring health...
Ngày tải lên: 17/02/2014, 10:20
Tài liệu Báo cáo khoa học: A role of miR-27 in the regulation of adipogenesis ppt
... or without hematoxylin staining. In hypoxia experiments, 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati, OH, USA) constantly maintained at 1% O 2 . Culture medium ... lgặmL )1 insulin, 1 lm dexamethasone, and 0.5 mm IDM). Cells were then maintained in DMEM con- taining 10% fetal bovine serum and 1 lgặmL )1 insulin. The medium was replaced every other day. ... cultured in DMEM containing 10% fetal bovine serum. OP9 cells were grown in aMEM containing 20% fetal bovine serum. C2C12 cells were maintained in DMEM containing 10% fetal bovine serum. Adipogenic...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Research " The Dissertation Committee for Fang Yin Certifies that this is the approved version of the following dissertation: Business Value of Information Technology in the Internet Economy " doc
... Faculty of the Graduate School of The University of Texas at Austin in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy The University of Texas at Austin ... and the type of electronic 16 labor, and where t is the number of years in business. t is included in the model to control for the maturity of a company. Companies operating in the Internet ... migrate many of their business activities to the Internet, can they also benefit from insights regarding productive and unproductive activities in an online world? In the late nineties, online traffic...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: The role of N-glycosylation in the stability, trafficking and GABA-uptake of GABA-transporter 1 Terminal N-glycans facilitate efficient GABA-uptake activity of the GABA transporter pptx
... N-linked oligosaccharide side-chains are important for the GABA transport activity. 1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into the role of the terminal ... results in reduction of the stability of GAT1 In order to study the in uence of the N-glycosylation and N-linked oligosaccharides on protein stability, the intracellular decay time of the GAT1, ... for the stability of this protein, but the terminal structure of the N-glycans is not. Defective N-glycosylation reduces the trafficking of GAT1 to the plasma membrane In order to study the in uence...
Ngày tải lên: 19/02/2014, 17:20
Báo cáo khoa học: Leishmania donovani methionine adenosyltransferase Role of cysteine residues in the recombinant enzyme pptx
... to determine the functional role of the amino acid residues involved in each. The present report describes the cloning and subsequent functional refolding, using a bacterial expression system, of the ... a donor of propylamine groups in the metabolism of spermidine and spermine [2] and an intermediary in the trans-sulfuration pathway to cysteine, one of the three amino acids involved in glutathi- one ... domain of each subunit, in the interface between the two dimers compri- sing the tetrameric structure [29]. This domain contains five cysteine residues, two of them (Cys35 and Cys61) forming a...
Ngày tải lên: 08/03/2014, 08:20
The Role of Rainfed Agriculture in the Future of Global Food Production doc
... trees. The largest plant height was obtained using linings of stone and marble. During the first year of the study, when the lining served only as mulch due to lack of rainfall, an increase of ... to improve the HI of wheat, 9 The harvest index is defined as the ratio of grain to total crop biomass” (Cassman 1999). The Role of Rainfed Agriculture in the Future of Global Food ... Considerations of risk may be very important in the initial decision of whether to adopt rainwater harvesting at an individual farm level. Especially due to the low economic status of many of the farmers...
Ngày tải lên: 09/03/2014, 00:20
The Role of Bank Capital in the Propagation of Shocks potx
... differences in bank capitalization. Technology shocks Figure 4 depicts the effects of a one-standard-deviation negative technology shock in two economies. The full lines describe the responses of the ... to the banking sector The previous subsection analyzes how the banking sector, through the dynamics of bank capital, affects the transmission of shocks. In recent years, however, episodes of increased volatility ... to them. In this context, this subsection considers the effects of a shock that arises in the banking sector of our model and leads to exogenous declines in bank net worth. Following the theoretical...
Ngày tải lên: 15/03/2014, 10:20
Báo cáo khoa học: Kinetic and crystallographic analyses of the catalytic domain of chitinase from Pyrococcus furiosus – the role of conserved residues in the active site pdf
... number of amino acid residues contribute to the binding of the substrate by hydrogen bonding and ⁄ or hydrophobic interactions. Using the ligplot program [31], we investigated the specific interactions ... 2010) doi:10.1111/j.1742-4658.2010.07685.x The hyperthermostable chitinase from the hyperthermophilic archaeon Pyrococcus furiosus has a unique multidomain structure containing two chi- tin-binding domains and two catalytic domains, ... identical, but the D524A mutation resulted in a remarkable change in the conformation of the Glu526 side-chain. The 2F obs ) F calc electron density of the Glu526 side-chain in the D524A–substrate...
Ngày tải lên: 15/03/2014, 11:20