... models or inthe surroundings of the mutated residues. The models of RNase A covalent dimers in the exchanging conformation were prepared using the 7RSA structure and the structure of exchanging BS-RNase(1BSR) ... through the exchange between subunits of either their N-ter-minal, or their C-terminal ends [33–35]. We foundthat the behavior with the model membrane of the dimer in which the C-terminal ends ... wereprepared using models of these dimers. The models of RNase A covalent dimers inthe nonexchanging conforma-tion were prepared using the 7RSA structure and the struc-ture of nonexchanging BS-RNase...
... However, the mechanistic roles of several of these residues are not described well and there is no example of mutational analysis of all of these residues inthe sameenzyme.To obtain an insight into ... by the surrounding residues. The results so far indicate thatlarger parts ofthe polar core ofthe catalytic TIM barrel of family 18 chitinases play a role during catalysis. It will thusbe interesting ... conformations. The Asp142 equiv-alent inthe family 18 chitinase from Coccidioides immitiswas found to be inthe ÔdownÕ position inthe apoenzymeand inthe ÔupÕ position inthe complex with allosamidin...
... whenliving in damp air). If the induration ofthe right lungwhich was observed in A.'s case really commenced at the time he experienced pain in rowing inthe Collegeraces, then possibly the ... participated in the struggle—nine ofthe cases resting on the evi-dence of men who themselves rowed and are still alive,and the remaining eight on reports received from some of the relatives of those ... havearisen originally from the bursting of a blood-vesselthrough his exertions in rowing, either inthe practicefor the Inter-University Race or inthe race itself."Some of these contemporaries...
... under the framework of financial globalization.II. Review of Institution Building inthe Financial SectorA. Main driving forces of institution buildingA review ofthe developments of China’s financial ... performance.2. Roleofthe central bank The central bank has played a roleof policy designing and implementation in the process of institution building inthe financial sector. The promulgation ofThe ... source of financing for enterprises, if their owncapital is excluded. In summary, the deepening process ofthe financial markets has rendered the dominance ofthe banking sector inthe financial...
... (39 ). The renewed interest in examining the indications for BCG vaccination in the United States included consideration ofthe wide range of vaccine efficacies deter-mined by clinical trials ... toexplore the sources ofthe heterogeneity inthe efficacy ofthe BCG vaccine reported in the individual studies. Using a model that included the geographic latitude of the study site and the data ... <5 mm indurationafter skin testing with 5 TU of PPD tuberculin. The Tice strain of BCG is administeredpercutaneously; 0.3 mL ofthe reconstituted vaccine is usually placed on the skin in the lower...
... since the mid-1990s says more about the local economies in which they operate than the viability of community banking as abusiness model.Implications of advances in information-gathering technology ... an important roleinthe financial system of the U.S. economy. They complement theroleof large banks by special-izing in relationship banking and providing credit to smallbusinesses—a sector ... “Small Business Lending and the Changing Structure ofthe Banking Industry,” Journal of Banking andFinance, August.Sullivan, Richard J. 2000. “How Has the Adoption of Internet Banking AffectedPerformance...
... cost-effective way of as-sessing health risks; the identification of all risk points along the chain of events from the origin ofthe wastewater to the consumption ofthe produce (e.g. the farm-to-fork ... Ho-epner,2008). There is scope to improve the sustainability of the desalination process. The process results inthe discharge of a concentrated brine into the receiving waters. Temperature and salinity ... approach ofthe HAPPC meth-od in food safety); the design of a combination of health risk management mea-sures, to be applied along the same chain of events, with the aim of ensuring health...
... or without hematoxylin staining. In hypoxia experiments, 3T3-L1 cells were maintained in a hypoxia chamber (Invivo 400; Ruskinn Inc., Cincinnati,OH, USA) constantly maintained at 1% O2. Culturemedium ... lgặmL)1insulin, 1 lm dexamethasone, and0.5 mm IDM). Cells were then maintained in DMEM con-taining 10% fetal bovine serum and 1 lgặmL)1insulin. The medium was replaced every other day. ... cultured in DMEM containing 10% fetal bovineserum. OP9 cells were grown in aMEM containing 20%fetal bovine serum. C2C12 cells were maintained in DMEMcontaining 10% fetal bovine serum.Adipogenic...
... Faculty ofthe Graduate School of The University of Texas at Austin in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy The University of Texas at Austin ... and the type of electronic 16labor, and where t is the number of years in business. t is included inthe model to control for the maturity of a company. Companies operating inthe Internet ... migrate many of their business activities to the Internet, can they also benefit from insights regarding productive and unproductive activities in an online world? In the late nineties, online traffic...
... N-linked oligosaccharide side-chainsare important for the GABA transport activity.1-Deoxymannojirimycin inhibits the GABA-uptake of GAT1 In order to gain further insight into theroleof the terminal ... results in reduction of the stability of GAT1 In order to study thein uence ofthe N-glycosylationand N-linked oligosaccharides on protein stability, the intracellular decay time ofthe GAT1, ... for the stability of this protein,but the terminal structure ofthe N-glycans is not.Defective N-glycosylation reduces the trafficking of GAT1 to the plasma membrane In order to study thein uence...
... todetermine the functional roleofthe amino acid residuesinvolved in each. The present report describes the cloningand subsequent functional refolding, using a bacterialexpression system, ofthe ... a donor of propylaminegroups inthe metabolism of spermidine and spermine [2]and an intermediary inthe trans-sulfuration pathway tocysteine, one ofthe three amino acids involved in glutathi-one ... domain of eachsubunit, inthe interface between the two dimers compri-sing the tetrameric structure [29]. This domain containsfive cysteine residues, two of them (Cys35 and Cys61)forming a...
... trees. The largest plant height was obtained using linings of stone and marble. During the first year ofthe study, when the lining served only as mulch due to lack of rainfall, an increase of ... to improve the HI of wheat, 9 The harvest index is defined as the ratio of grain to total crop biomass” (Cassman 1999). The Roleof Rainfed Agriculture inthe Future of Global Food ... Considerations of risk may be very important inthe initial decision of whether to adopt rainwater harvesting at an individual farm level. Especially due to the low economic status of many ofthe farmers...
... differences in bank capitalization. Technology shocksFigure 4 depicts the effects of a one-standard-deviation negative technology shock in two economies. The full lines describethe responses ofthe ... to the banking sector The previous subsection analyzes how the banking sector, through the dynamics of bankcapital, affects the transmission of shocks. In recent years, however, episodes of increasedvolatility ... to them. In this context, this subsection considers the effects of a shock that arises inthe bankingsector of our model and leads to exogenous declines in bank net worth. Following the theoretical...
... number of amino acid residues contribute to the binding of the substrate by hydrogen bonding and ⁄ or hydrophobicinteractions. Using the ligplot program [31], weinvestigated the specific interactions ... 2010)doi:10.1111/j.1742-4658.2010.07685.x The hyperthermostable chitinase from the hyperthermophilic archaeonPyrococcus furiosus has a unique multidomain structure containing two chi-tin-binding domains and two catalytic domains, ... identical, but the D524A mutation resulted in aremarkable change inthe conformation ofthe Glu526side-chain. The 2Fobs) Fcalcelectron density of the Glu526 side-chain inthe D524A–substrate...