derivation of induced pluripotent stem cells by lentiviral transduction

... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent...

Ngày tải lên: 09/09/2015, 18:56

... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8 ... zone of cartilage could be clearly separated by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the ... 6-carboxyfluorescein by the esterases of living cells to label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5...

Ngày tải lên: 09/08/2014, 14:22

... embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced pluripotent stem ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... pluripotent and differentiated cells Nat Biotechnol 2010, 28:1079-1088 54 Sha K, Boyer LA: The chromatin signature of pluripotent cells In StemBook Edited by Girard L Massachusetts: Harvard Stem...

Ngày tải lên: 11/08/2014, 12:21

... generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic fibroblasts by ... fibroblasts to induced pluripotent stem cells Nature 2009, 458:766-770 Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K: Virus-free induction of pluripotency and subsequent excision of reprogramming ... JA: Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent...

Ngày tải lên: 14/08/2014, 21:20

... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 Immunocytochemical ... Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from fibroblasts to Induced ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent...

Ngày tải lên: 30/09/2015, 10:13

... the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these ... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... monogenic and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... GQ, Porteus MH, Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F, ... the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita J, Okeda...

Ngày tải lên: 11/08/2014, 12:21

... study of embryonic stem cells TE N Hattori and K Shiota ICM PGC TS cells Placental cells 77 ES cells 49 EG cells Embryonic cells Fig Epigenetic distances between ES cells and other stem cells ... Promotion of trophoblast stem cell proliferation by FGF4 Science 282, 2072– 2075 12 Matsui Y, Zsebo K & Hogan BL (1992) Derivation of pluripotential embryonic stem cells from murine primordial germ cells ... By contrast, TS cells derived from the trophectoderm (TE) of blastocysts contribute only to placenta Although there is an apparent ED between ES cells and EG cells, the ED of TS cells to ES cells...

Ngày tải lên: 23/03/2014, 07:20

... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human -induced pluripotent ... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... sequencing; ESC, embryonic stem cell; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; H3K4me3, histone H3 lysine trimethylation; iPSC, induced pluripotent stem cell, lincRNA,...

Ngày tải lên: 11/08/2014, 12:21

... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen -induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD 3induced proliferation of CD4+ T cells ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... proliferation of CD4+ T cells was analyzed by detection of CFSE dilution by flow cytometry The percentage inhibition (100 × [(percentage of proliferating CD4+ cells not treated with MSCs – percentage of...

Ngày tải lên: 12/08/2014, 11:23

... the percentage of positive cells and the mean fluorescence intensity (MFI) of the population were determined FACS sort of lacZ-positive cells The lentiviral siRNA vector-transduced cells co-expressing ... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the...

Ngày tải lên: 13/08/2014, 13:20

... Adult stem cells Adult stem cells also known as somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of pluripotent ... blood cells and platelets, while skin stem cells give rise to the various types of skin cells Some recent reports suggest that adult stem cells such as haemopoietic stem cells, neuronal stem cells...

Ngày tải lên: 16/09/2015, 15:55

... expression of SSEA-3 was indicated by green fluorescence under a wavelength of 490 nm (FITC), while positive expression of TRA-1-81 was indicated by red fluorescence under a wavelength of 570 nm ... cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor FISH ... conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low...

Ngày tải lên: 31/10/2012, 16:57

... possibility of a saturable mechanism of entry (Fig 1A); identical results were obtained with a control ODN (not shown) Examination of the cells by light microscopy showed that untreated cells, cells ... activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with hpST3dODN was analysed in SW480 cells transfected ... results are expressed as a percentage of dead cells (B) Cleavage of PARP, induced by 24 or 48 h of treatment with IFN-c at 5, 20, 100 and 200 ngÆmL)1 was analysed by western blotting using anti-cleavedPARP...

Ngày tải lên: 18/02/2014, 08:20

... differentiation of murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells to gen- ... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... primer that hybridizes just downstream of The commitment of pluripotent stem cells to successively less plastic progenitors and, finally, differentiated cells exhibiting stable expression patterns...

Ngày tải lên: 07/03/2014, 12:20

... degree of Nat5 inhibition observed in the cells The cell cycle disruption induced by the downregulation of Nat5 expression is induced by the activation of p53 that induces an increase of p21 ... Bickle2 Laboratory of Dr Ettrich, Institute of Physical Biology of USB and Institute of Landscape Ecology of AS CR, Nove Hrady, Czech Republic, 2Laboratory of Dr Bickle, Department of Microbiology, ... formation of a sulfenic acid intermediate with concomitant release of one mol of Met per mol of Msr, (ii) formation of an intramonomeric disulfide bond, the rate of which has been shown to be limited by...

Ngày tải lên: 30/03/2014, 20:20

... expressed in hES cells (Figure 7) The expression of OCT4 by hES cells was upregulated by 12fold, LEFTY1 by 70-fold and TDGF1 by 19-fold compared to EB Compared to adult cells, expression of OCT4 in ... embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific ... increased by 4,324-fold, LEFTY1 by 769-fold, and TDGF1 by 2,443-fold We did not find that Nanog was upregulated in hES cells by microarray analysis, and by qRT-PCR its expression was increased by only...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... temporal change of bone marrow fat cells in the process of steroid-associated-osteonecrosis development Chinese Journal of Osteoporosis 2007 Cui Q, Wang GJ, Balian G: Pluripotential marrow cells produce...

Ngày tải lên: 20/06/2014, 00:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according...

Ngày tải lên: 20/06/2014, 04:20