dependently elevated without significant changes in il 17 production
Báo cáo y học: "Elevated extracellular matrix production and degradation upon bone morphogenetic protein-2 (BMP-2) stimulation point toward a role for BMP-2 in cartilage repair and remodeling" pps
... stained immunohistochemically for VDIPEN (Figure 1i–l) BMP-2 initially induced an increase in VDIPEN staining on day in patellar cartilage In tibial cartilage, elevated levels of VDIPEN staining ... also leads to increased VDIPEN staining (k) and NITEGE staining (q,p) Arrows point to intense staining around chondrocytes FastG, fast green; SafO, safranin O Co-incubation of several dilutions of ... differences in safranin O staining intensity between BMP-2-exposed cartilage and controls were observed by mere visual investigation Therefore, the safranin O staining intensity was scored in patellar...
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Báo cáo y học: "High level expression of apoptosis inhibitor in hepatoma cell line expressing Hepatitis
... The initiator caspase group includes caspase-2, caspase-8, caspase-9, and caspase-10, having long NH2-terminal prodomains, which interact with adapter molecules to form a death-inducing signaling ... how these proteins contribute to the carcinogenesis is still in the investigation The involvement of these proteins in the NF-kB related signal transduction pathway that results in the cell apoptosis ... persistently HBV expressing cell line HepG2.215 and its parent cell line, a non-HBV expressing cell line HepG2 We found that cIAP1 and cIAP2 were clearly increased in the HBV expressing cells but XIAP...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... may work in SC cell lines and AD cell lines, respectively, in this study The third connection including TGM2, IL1 B, and PLAU was suggested to be tumor invasion It was reported that IL1 B increased ... calcium-binding protein P, may be a good biomarker for AD cells The expression ratio of S100P in cancer/normal cells was high in AD samples and low in SQ samples In our study, all three AD cell lines ... which might be involved in establishing a barrier to tumor spreading [27] The third connection may not be effective in cell lines in this study, because TGM2 was rather downregulated in this study...
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Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
... failed to elevate the EGFP intensity in the reporter vector containing a mutation of the first miR-23a binding region, but could still elevate the EGFP intensity in the reporter vectors containing ... Relative IL6 R mRNA level A L.-H Zhu et al 0.6 0.4 0.2 pSilencer/ pSilencer/ NC sh -IL6 R pSilencer/ pSilencer/ NC sh -IL6 R * 90 80 70 60 50 40 30 20 10 pSilencer/NC pSilencer/sh -IL6 R Fig Knockdown of IL6 R ... EGFP -IL6 R 3¢ UTR reporter vector bearing the potential miR-23a binding site in the fluorescent reporter, and found an increase in EGFP intensity after blocking miR-23a and a decrease in EGFP intensity...
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Tài liệu Báo cáo khoa học: Down-regulation of heme oxygenase-2 is associated with the increased expression of heme oxygenase-1 in human cell lines docx
... maintained in HO-2– ⁄ – mice through appropriate resetting of HO-1 expression In contrast with HO-1 expression, expression of HO-2 mRNA and protein was not increased in several human cell lines ... cells were maintained in Dulbecco’s modified Eagle’s medium (Sigma) YN-1 cells were maintained in Iscove’s modified Dulbecco’s medium (Sigma), and K562, KG1 and Jurkat cells were maintained in RPMI-1640 ... K562, Jurkat, and H146, were maintained in suspension culture (Fig 1), although HO-1 is expressed in two other cell lines, YN-1 and KG1, that were also maintained in suspension culture These results...
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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf
... [27,34] were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM) (Sigma) HeLa and HepG2 cells were maintained in DMEM Each medium contains 10% heat-inactivated FBS, penicillin G Total RNA ... described in Fig 1B The intensities of the signals in (A) and (C) were quantified, and the intensity representing HO-1 or HO-2 protein was normalized with respect to the intensity for the a-tubulin signal ... expression levels of HO-1 and HO-2 proteins in YN-1 cells (B) and those of HO-2 protein in K562 cells (D) The intensity representing HO-1 or HO-2 protein at the time (0 h) is considered to be...
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Tài liệu Báo cáo khoa học: Altered expression of CD1d molecules and lipid accumulation in the human hepatoma cell line HepG2 after iron loading pptx
... ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured without and with iron as indicated (D) Perls’ staining of cytospins ... the increase in the prooxidant state caused by iron loading has an impact in protein integrity, as indicated by an increase in protein-bound acrolein adducts at the cell surface, which point ... as determined by the increase in mean fluorescence intensity (MFI) (Fig 1C) As shown in Fig 1B, permeabilization with saponin did not increase background staining when rabbit immunoglobulins were...
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Tài liệu Báo cáo khoa học: Identification of different isoforms of eEF1A in the nuclear fraction of human T-lymphoblastic cancer cell line specifically binding to aptameric cytotoxic GT oligomers ppt
... sequence, increasing the number of basic residues of lysine, arginine and histidine, could occur However it seemed probable that the eEF1A isoforms could act as a controlling event in maintaining the ... Eukaryotic EF1A is a protein belonging to the GTPbinding elongation factor family, which promotes the GTPdependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis and ... microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 20 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head...
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Tài liệu Báo cáo khoa học: Insulin/protein kinase B signalling pathway upregulates metastasis-related phenotypes and molecules in H7721 human hepatocarcinoma cell line pptx
... alteration in PKB protein was similar to that in PKB activity (Fig 7), suggesting that the insulininduced PKB protein was almost fully phosphorylated and activated Whether insulin was internalized ... that of PKB protein and activity in the insulin treated and S-PKB cells, indicating that SLex might be regulated by other insulin-induced factor(s) in addition to PKB signalling pathways It is ... HUVECs elevated only very slightly Cell adhesion to laminin was increased with the concentrations of the coated laminin, and enhanced after insulin treatment at different concentrations of laminin...
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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc
... value in Table 1) is taken as 100% (B) Basal incorporation of BrdU into proliferating LXF-289 cells in the presence of the inhibitors for the kinases calcium ⁄ calmodulin-dependent protein kinase ... & Burnstock G (2005) Changes in P2Y2 receptor localization on adrenaline- and noradrenalinecontaining chromaffin cells in the rat adrenal gland during development and aging Int J Dev Neurosci 23, ... SB203580) or phosphatidylinositol-3-kinase (PI3K) (wortmannin), indicating that each pathway seems to be involved in ATP-mediated inhibition of LXF-289 cell proliferation (Table 2) Inhibition of the...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
... immunoprobed for p21waf1, cyclin B, cyclin D, CDK and CDK Combining CDO with any chemotherapeutic drug resulted in changes in protein levels that appeared to be similar, irrespective of the chemotherapy ... that CDO alone did not increase the extent of staining However, in cells that had been cocultured with CDO and a cytotoxic drug, staining was significantly increased, indicating the presence of senescence ... provide a possible model of drug interaction in GEO cells, which involves the complex interaction of proteins involved in cell cycle regulation To recapitulate, combining CDO with cytotoxic chemotherapy...
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Báo cáo khoa học: 3 Cdt1 and geminin are down-regulated upon cell cycle exit and are over-expressed in cancer-derived cell lines potx
... geminin mRNA and protein reveal a similar distribution along the gastrointestinal tract, localizing mainly in the proliferating zone of the gut epithelium Cdt1 and geminin are over-expressed in ... full-length Cdt1 and geminin (HisT7-geminin and His-geminin, amount of recombinant protein run in each lane in ng is indicated) and immunoblotted with anti-Cdt1 and anti-geminin Ig See Materials ... differences in relative amounts of Cdt1 and its inhibitor in different cell lines It would be interesting to investigate whether geminin and/or Cdt1 levels or the ratio of Cdt1 to its inhibitor geminin...
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Báo cáo Y học: Proteasome-driven turnover of tryptophan hydroxylase is triggered by phosphorylation in RBL2H3 cells, a serotonin producing mast cell line pptx
... 32P-Loading was further continued for 120 in the presence of protein-kinase inhibitors or proteinphosphatase inhibitors Cells were then rinsed with NaCl/Pi and disrupted with 1% NP-40 in 50 mM ... were then disrupted with 1% NP-40 in 50 mM Tris/HCl (pH 7.8) containing the protease inhibitor cocktail (32P): In order to examine 32P-incorporation into proteins, the cell extracts were subjected ... digested away in the absence of proteasome inhibitors (lane in Fig 1B, 32P vs WB) Inhibition of TPH degradation in the cell-free proteasome system by protein kinase inhibitors We examined the involvement...
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Báo cáo Y học: The SK-N-MC cell line expresses an orexin binding site different from recombinant orexin 1-type receptor pptx
... for orexin B 18–28 serine and the C-terminal methionine The conformational in uence of the C-terminal part of the orexins remains unclear The introduction of a helix inducers into C-terminal peptide ... of orexin A via the lateral hypothalamus and after administration of orexin A 23–33 and orexin A (A) Food intake after administration of orexin A 23–33 (B) Sequencing To study orexin binding we ... 125 h-orexin A h-orexin B [L28]h-orexin B h-orexin A 23–33 [G23]h-orexin A 23–33 h-orexin B 18–28 Modafinil EXBN8016BSa NPY a 1-(4-N,N-dimethyl-aminophenyl)-3-chinolin-4yl-urea) [27] were injected...
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Báo cáo khoa học: Internalization of cystatin C in human cell lines docx
... convincingly demonstrate that the cysteine protease inhibitor cystatin C is internalized in all five of the cancer cell lines investigated in this study Using confocal microscopy, western blotting ... different abilities in cystatin C uptake The inhibition capacity in lysates of cells incubated for 24 h without labelled cystatin C showed a level of 200 pmol cysteine protease inhibitorÆmg protein)1 ... protease, legumain, because of removal of the key amino acid in the legumain-binding site of cystatin C [22] The amino acid substitutions reside in opposing parts of the cystatin C molecule, which...
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Báo cáo khoa học: Interaction of G-rich GT oligonucleotides with nuclearassociated eEF1A is correlated with their antiproliferative effect in haematopoietic human cancer cell lines potx
... growth inhibition effect was observed for GT-G4 The ability of these sequences to bind to proteins forming the CRC of 45 ± kDa was checked by UV cross-linking assays in competition experiments As illustrated ... temperature of annealing, and frequently different coexisting competing forms In particular GRO29A needs 56 h annealing at 60 °C in 140 mm KCl to assume a G-quartet containing structure [26] In agreement ... were equilibrated in TEN 1000 For protein binding, the beads were preincubated with 0.5 mgÆmL)1 BSA in TEN 100 for 10 The beads were washed thrice with TEN 100 and then equilibrated in 20 mm...
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Báo cáo khóa học: Endoplasmic reticulum-associated degradation of glycoproteins bearing Man5GlcNAc2 and Man9GlcNAc2 species in the MI8-5 CHO cell line docx
... & Braakman, I (2000) Truncated N-glycans affect protein folding in the ER of CHO-derived mutant cell lines without preventing calnexin binding Glycobiology 10, 77–87 Duvet, S., Chirat, F., Mir, ... containing one Glc, nine Man, and two GlcNAc residues M4, M5, M8 and M9 indicate oligomannosides containing four, five, eight and nine Man residues and two GlcNAc residues, respectively X indicates ... proteins during a pulse-chase experiment MI8-5 CHO cells were pulse-labeled with [2-3H]Man for h after 40 of preincubation in 0 .175 mM Glc (T0 h) and chased in the culture medium containing mM...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot
... quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, 248–254 Prohaska, J.R & Ganther, H.E (1976) Selenium and glutathione peroxidase in developing rat brain J Neurochem ... cells were incubated in NaCl/Pi for 90 min, the intracellular fluorescence signal dramatically increased in all cases (Fig 9, Table 1) The increases, in comparison to the signals observed in standard ... Reversal of agerelated increase in brain protein oxidation, decrease in enzyme activity, and loss in temporal and spatial memory by chronic administration of the spin trapping compound N-tert-butyl-aphenylnitrone...
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Báo cáo Y học: GPI-microdomains (membrane rafts) and signaling of the multi-chain interleukin-2 receptor in human lymphoma/leukemia T cell lines doc
... of the IL- 2R chains with lipid rafts (containing CD48) may have several functional consequences in T cells First, rafts may concentrate the a chains (CD25) in the vicinity of signaling IL- 2R b ... cytokine [11] Second, the GPI microdomains can also concentrate other cytokine receptor a chains (e.g IL- 4Ra IL- 7Ra or IL- 15Ra) in the locality of common c chains shared by them for signaling ... cell line associated with HTLV-1 and is deficient in the signaling IL- 2Rb and c subunits [25] All cell lines were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, penicillin...
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Báo cáo khoa học: An estrogen receptor a suppressor, microRNA-22, is downregulated in estrogen receptor a-positive human breast cancer cell lines and clinical samples pptx
... attached monolayers in DMEM containing 10% fetal bovine serum Cells were harvested by treatment with trypsin and counted every 24 h in triplicate (D) Single-cell suspensions containing 10 000 cells ... Medical College (Beijing, China) and maintained in our laboratory HEK293T (American Type Culture Collection, Manassas, VA, USA) and MCF-7 cells were maintained in 10% fetal bovine serum-supplemented ... directly binding to ERa 3¢-UTR, and inhibited ERa expression through both destabilizing mRNA and inhibiting translation FEBS Journal 277 (2010) 1684–1694 ª 2010 The Authors Journal compilation...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25