... latch may be the last event to occur being triggered not by direct disruption of the interface but as a consequence of the Kd being rather large At low pH, the helical latch could be the rate limiting ... or by the presence of protons We have shown that the helix latch and the G2 to G4–6 interaction in general is not reduced by low pH It is possible therefore that in pH-activated gelsolin, the helical ... with the poor accessibility of the tryptophan suggested by the position of the maximum wavelength of the fluorescence (336 nm at pH 5.7 and 342 at pH 6.8 in EGTA) The results obtained using the...
Ngày tải lên: 23/03/2014, 21:20
... coordinating the incoming triphosphate In the process, Arg72 donates hydrogen bonds to the a-phosphate and the ε-amino group of Lys65 donates hydrogen bonds to the g-phosphate These events lead to the ... reversion) These results suggest that L74I change in the background of K65R leads to an RT which is much more stable as compared to RT with the K65R+L74V mutations In order to validate the reversion ... http://www.virologyj.com/content/8/1/33 K65R and L74V and the rare occurrence of K65R+L74V on the same HIV genome [6,27] may be related to the observed attenuation of the virus in the presence of these mutations [5,7,12,15]...
Ngày tải lên: 11/08/2014, 21:21
Tài liệu Importing the Law in Post-Communist Transitions The Hungarian Constitutional Court and the Right to Human Dignity pptx
... 2.1 The Right to Life and Human Dignity 2.2 Human Dignity and the General Personality Right (a) The Right to Free Fulfilment of the Personality (b) The General Freedom of Action (c) The Right to ... determining not only their type of regime but also their very structure, the workings of their economies and of their law By the late 1980s, the planned economy of many of them had deteriorated to the ... questionable as to their content and their extent: they will inevitably include a disturbing case which does not fit with the rest of the category, but which nevertheless cannot be excluded In other words,...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Cities for All Proposals and Experiences towards the Right to the City docx
... the city to the right to mobility, or in other words, the right to accessibility The Necessary Ambiguity of the Right to the City The reduction of the right to the city to the right to accessibility ... the right to residential mobility replaces the right to housing, reducing it to another commodity, and the right to the city is reduced entirely to the right to access to the city Right to Proximity ... recognition is the recent inclusion of the right to the city in the constitutions of Ecuador and Bolivia The Dimensions and Components of the Right to the City The right to the city is: • The right to habitat...
Ngày tải lên: 25/02/2014, 01:20
The Right to Health pdf
... include the rights to food, to water, to an adequate standard of living, to adequate housing, to freedom from discrimination, to privacy, to access to information, to participation, and the right to ... IS THE RIGHT TO HEALTH? A. Key aspects of the right to health • The right to health is an inclusive right. We frequently associate the right to health with access to health care and the building ... such as the right to life, the prohibition on torture and other cruel, inhuman and degrading treatment, and the right to family and private life Finally, the right to health or the right to health...
Ngày tải lên: 05/03/2014, 10:20
Report of the Special Rapporteur on the promotion and protection of the right to freedom of opinion and expression, Frank La Rue* ppt
... hold the persons responsible to account, and adopt measures to prevent such recurrence in the future F Inadequate protection of the right to privacy and data protection 53 The right to privacy ... of the gravest forms of restriction to the right, as it not only creates a “chilling effect”, but also leads to other human rights violations, such as arbitrary detention and torture and other ... 47 The Special Rapporteur commends such initiatives to enhance the responsibility of Internet intermediaries to respect human rights To avoid infringing the right to freedom of expression and the...
Ngày tải lên: 06/03/2014, 21:20
The Right to Property in Commonwealth Constitutions potx
... not only to the value of the land to be purchase or taken by the promoters but also to the damage, if any, to be sustained by the owner of land by reason of the severing of the land from the ... proprietary right to photocopies of con®dential information that had been leaked to The Guardian newspaper The newspaper resisted the claim to the photocopies on the basis of section 10 of the Contempt ... constitutional rights to property Singapore has a constitutional bill of rights, but it does not include a right to property Other countries have enacted bills of rights that include rights to property, but...
Ngày tải lên: 23/03/2014, 05:23
The Protection of the Right to Education by International Law doc
... protect other human rights, often economic, social and cultural rights, the category of human rights to which the right to education is generally considered to belong Most notably, the right to education ... submitted by the Special Rapporteur of the Commission on Human Rights on the Right to Education) (2003b) Tomaevski, K., Economic, Social and Cultural Rights: The Right to Education: Mission to the United ... submitted by the Special Rapporteur of the Commission on Human Rights on the Right to Education) (2003a) Tomaevski, K., Economic, Social and Cultural Rights: The Right to Education: Mission to Indonesia...
Ngày tải lên: 30/03/2014, 10:20
báo cáo hóa học:" Reduced renal function is associated with progression to AIDS but not with overall mortality in HIV-infected kenyan adults not initially requiring combination antiretroviral therapy" potx
... chose not to include weight in these initial models as previous studies suggested that the inclusion of weight in the Cockcroft-Gault formula, but not in the simplified MDRD formula, led to significant ... with the median (IQR) time from cART initiation to death being 19 (7-42) weeks Overall, 25.1% were lost to follow up during the study period, which is similar to the lost -to- follow-up rates in other ... antiretroviral therapy (cART) [2] In the midst of the region’s struggle to provide cART to individuals meeting these conservative criteria for treatment, WHO has recommended raising the CD4 cell...
Ngày tải lên: 20/06/2014, 08:20
Báo cáo y học: "Cartilage degradation is fully reversible in the presence of aggrecanase but not matrix metalloproteinase activity" pdf
... understanding of the molecular events leading to cartilage destruction Many alternative hypotheses and conditions, including but not limited to the following, need to be investigated: (a) whether the aggrecanases ... the S-GAG levels released to the conditioned medium for the anabolic periods (days to 21, 11 to 25, and 17 to 31) (that is, during the 14day anabolic period subsequent to the catabolic insult) As ... activities are highly researched topics that are only beginning to be partly understood The data not provide the complete answer but hopefully add a piece of the highly complicated puzzle Most...
Ngày tải lên: 09/08/2014, 10:23
Báo cáo y học: " Hyperresponsiveness to inhaled but not intravenous methacholine during acute respiratory syncytial virus infection in mice" pot
... had to cross the airway epithelium to reach the underlying ASM, and implicates the integrity of the epithelial layer as important in the response to inhaled agonists Epithelial mechanisms are the ... haematoxylin and eosin Measurement of cytokines and mediators in BALF In order to characterise the primary inflammatory and cytokine response to RSV infection, we chose the appropriate kit to measure ... The peak-topeak amplitude of the oscillatory signal was 50% of tidal volume The flexiVent ventilator was used both for regular ventilation and for delivery of the oscillatory signal without the...
Ngày tải lên: 12/08/2014, 18:20
Báo cáo y học: " Success of organ donation after out-of-hospital cardiac death and the barriers to its acceptance" docx
... costs and respecting the body after death During resuscitation, Emergency Medical Service rescuers must focus completely on their task of restarting the heart to achieve the return of spontaneous ... continuing success of the Madrid and Barcelona out-of-hospital UDCD programs, demonstrate the viability and reproducibility of such protocols The IOM conservatively estimated that in the US about 22,000 ... could obviate the waiting list for kidney transplants [11] Thus, societies have the medical and moral obligation to develop UDCD programs Competing interests The authors declare that they have no...
Ngày tải lên: 13/08/2014, 19:20
Báo cáo y học: "The right to be wrong" potx
... of reviewers, too) It’s also easy to fall in love with a hypothesis, and to hang onto it longer than the data say you should These aren’t good things for a scientist to do, but they shouldn’t ... explanations turn out not to be the answer And we should not be afraid to abandon our chosen explanations and hypotheses when the preponderance of the evidence goes against them Nothing holds science ... incorrect, so they reward the incremental, safe projects at the expense of the bold and risky Scientists don’t want to be pilloried by their colleagues for having made a mistake, so they tend to the incremental,...
Ngày tải lên: 14/08/2014, 08:20
The Right to Religious Freedom in International Law Between Group Rights and Individual Rights Routledge Research in Human Rights Law
... commentators have noted, that the right to development is meaningless except as a right to be exercised by groups,29 although others have claimed that the right to development is not and cannot be ... accordance with their religion and belief This right is given in the context of the status of the child, but the right to organize the religious life of the family introduces a broader idea, of the family ... terms, the right was preserved The right to change belief was included in the UDHR While the 1981 Declaration does not expressly mention the right, it declares in Article 8, that nothing in the...
Ngày tải lên: 13/10/2016, 11:38
Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
... of apoptosis We further examined the character of cell death induced by VP2 or VP3 proteins To assess the contribution of apoptosis to toxicity, we evaluated the cleavage of both effector caspase ... tVP3–EGFP These results show that the levels of cytotoxicity of individual VP2 and VP3 variants correlate with their ability to induce apoptosis The highly toxic variants of VP2 and VP3 proved to be ... Cy, cytoplasm; Mit, mitochondria; Nu, nucleus To further characterize the induction of apoptosis caused by transient expression of VP2–EGFP and VP3– EGFP, and to determine the role of the mitochondrial...
Ngày tải lên: 16/02/2014, 09:20
Báo cáo khoa học: Protein crystallography for non-crystallographers, or how to get the best (but not more) from published macromolecular structures potx
... atoms are not included, their contribution is represented completely by the parent atom and its position tends to refine to the ‘center of gravity’ of both atoms As a result the geometry of the ... may be a good habit to have a look at the atomic coordinate section of the PDB file to see the level of the B-factors of the atoms that are in the most important fragments of the struc˚ ture If ... other RNase A-like enzymes The orientation of the His97 ring in the deposited structure (PDB code 2i5s) was determined, on the basis of the B-factors of the imidazole atoms, to be opposite to...
Ngày tải lên: 16/03/2014, 06:20
báo cáo khoa học: " Sgt1, but not Rar1, is essential for the RB-mediated broad-spectrum resistance to potato late blight" pptx
... until the end of the image selection The data report details the total number of counted particles, representing the total number of fluorescing sporangia on the leaf surface As the Phytophthora ... identified a potato EST, TC112395 (1461 bp), which showed 98% sequence similarity to the tobacco and tomato Sgt1 genes and 90% sequence similarity to the Arabidopsis thaliana Sgt1b gene Since the full-length ... with the potato Rar1 and Sgt1 gene probes The Southern hybridization results showed that the haploid potato genome contains only one copy of the Rar1 gene and two copies of the Sgt1 gene (data not...
Ngày tải lên: 12/08/2014, 05:20
Báo cáo y học: "Cystatin C influences the autoimmune but not inflammatory response to cartilage type II collagen leading to chronic arthritis development" ppsx
... protease inhibitors [26] These synthetic cysteine protease inhibitors are believed to have a protease inhibitory spectrum similar to that of cystatin C These studies, however, were not able to directly ... from the 129/Sv strain There is, then, even after 10 generations of backcrossing, a risk that the observed DTH and CIA effect is due to 129/Sv genes linked to the targeted locus, and not due to the ... not ideal but gives a starting point to start to unravel the complex mechanisms of cytstatin C In addition to the initial findings of altered IL-2 production, when we separated the APC from the...
Ngày tải lên: 12/08/2014, 15:22
Báo cáo y học: " The formation of cysteine-linked dimers of BST-2/tetherin is important for inhibition of HIV-1 virus release but not for sensitivity to Vpu" potx
... preventing exit of BST-2 from the ER or whether BST-2 can exit the ER and bypass the complex sugar modification machinery of the Golgi altogether On the other hand, we were able to detect high-mannose ... did not affect protein dimerization The fact that none of these mutations affected BST-2 function supports the notion that the loss of activity seen for the triple cysteine mutant is due to the ... particular the C13 and C3A mutants were highly sensitive to Vpu similar to wt BST-2 while expression of the C12 and C23 mutants appeared to be less affected by the presence of Vpu The reason for the...
Ngày tải lên: 12/08/2014, 23:22
Báo cáo y học: " Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2" ppsx
... does not bind to the viral Env protein, but to a cellular factor incorporated into the viral envelope For viruses produced in the kidney-derived cell line 293T, this factor was Page of 18 found to ... infectivity The augmentation of infectivity is due to the interaction of the virion-incorporated factors with their cognate receptors on HIV target cells, as exemplified by the up to 100-fold ... and contributed to the interpretation of experiments, JM, GS and RGS contributed to the design and the interpretation of experiments, SP planned and supervised the research and wrote the manuscript...
Ngày tải lên: 12/08/2014, 23:23