damages and their chemical and enzymatic repairs investigated by quantum chemical methods
HUMAN MILK OLIGOSACCHARIDES: CHEMICAL STRUCTURE, FUNCTIONS AND ENZYMATIC SYNTHESIS
Ngày tải lên :
28/08/2013, 16:28
... principle, HMOs can be synthesized by application of enzymes or by chemical approaches However, great effort nowadays has been put into enzymatic methods, because chemical methods still require multiple ... structure Fucα1-2Gal is H antigen, and H antigen is attached to GlcNAc with β1,3 and β-1,4-linkages to create H1 and H2 antigens, respectively A and B antigens are formed by adding a Gal or N-acetylgalactosamine ... applied in wide range of foodstuffs because of their known advantages: (i) They may be manufactured by extraction from plan sources, enzymatic synthesis and enzymatic hydrolysis of polysaccharides;...
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Báo cáo khoa học: Methylene analogues of adenosine 5¢-tetraphosphate Their chemical synthesis and recognition by human and plant mononucleoside tetraphosphatases and dinucleoside tetraphosphatases pot
Ngày tải lên :
16/03/2014, 14:20
... hydrolase by p4A and ppCH2ppA with that by ATP (p3A) and pCH2ppA, both compounds truncated by one phosphate (and one negative charge) relative to the nucleoside tetraphosphates Both ATP and its ... Recognition of p4A analogues as substrates by the p4A hydrolysing enzymes The preparation of intermediate ADP and ATP analogues followed standard methods Their conversion into p4A analogues called ... in Fig Other chemicals Unlabelled mono- and dinucleotides were from Sigma (St Louis, MO), and [3H]Ap4A (740 TBqÆmol)1) was purchased from Moravek, Biochemicals (Brea, CA) ppppRibose and ppCH2ppRibose...
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Aggregation enhanced two photon excited photoluminescence of noble metal nanoparticles and their chemical and biological applications
Ngày tải lên :
10/09/2015, 09:02
... Ag, are excellent candidates for developing sensors due to their unique chemical and physical properties.24, 37-43 They can be prepared by simple chemical reduction by NaBH4 and possess excellent ... important and urgent to develop detection methods for chemical and biological agents in many fields, which include environmental, biomedical sciences and more Noble metal nanoparticles, such as Au and ... attention in recent years owing to their unique physical and chemical properties as well as their applications in catalysis, optoelectonics, along with biological and chemical sensing 1.1.1 Localized...
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Soft coral (octocorallia, alcyonacea) diversity and distribution along a latitudinal environmental gradient and the role of their chemical defense against predatory fish the red sea
Ngày tải lên :
13/05/2016, 10:16
... coast and around various islands and diverse patch reefs Towards the South the slope of the coastal sea bed decreases slowly and fringing reefs around islands (Farasan banks and islands) and patch ... predators both through physical and chemical defense (Van Alstyne and Paul 1992; Koh et al 2000; O’Neal and Pawlik 2002) The combination of both physical and chemical defense was found to be ... Soft corals deter their predators by physical defense, chemical defense or both (La Barre et al 1986; Sammarco and Coll 1992; Van Alstyne et al 1994; O’Neal and Pawlik 2002) Chemical defense is...
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Assessment of pretreatments and enzymatic hydrolysis of wheat straw as a sugar source for bioprocess industry
Ngày tải lên :
05/09/2013, 15:28
... fractionation and superior kinetic advantages for enzymatic hydrolysis step 12 Enzymatic hydrolysis: enzyme systems and process overview Typical cellulose microfibril contains crystalline and amorphous ... wheat straw, and corn stover and analysis of their particle size distributions Powder Technol 2009, 193, 32-45 [54] Fan L.T., Lee Y.H., Gharpuray M.M The nature of lignocellulosics and their pretreatment ... assessment of bioethanol and valorization of side streams by anaerobic digestion and combustion Biotechnology for Biofuels 2008, 1, 14 [72] Kim S., Holtzapple M Lime pretreatment and enzymatic hydrolysis...
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Tài liệu Báo cáo khoa học: Bioinformatic and enzymatic characterization of the MAPEG superfamily doc
Ngày tải lên :
19/02/2014, 17:20
... filtered by a minimum size of 100 amino acid residues and flanked by start and stop codons These homology searches resulted in nearly 1000 redundant amino acid sequences which were followed by an ... terminated by a extension at 72 °C The PCR product was isolated by agarose gel electrophoresis, purified from the gel and digested with NdeI and HindIII The resulting product was gel purified and ligated ... concentration of mm and DNA and RNA were hydrolyzed by incubation with DNaseI (4 lgÆmL)1) and RNase A (4 lgÆmL)1) for 30 at °C with gentle stirring Cell debris was removed by centrifugation at...
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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf
Ngày tải lên :
07/03/2014, 11:20
... extended towards Cys183 in ChiC (and in the plant enzymes) Compared to the barley enzyme, ChiC and ChiG lack three loops (A, B and C) and a C-terminal extension (Figs and 4B) In addition, one other ... confirmed by the present analysis of enzymatic properties of ChiG, or by our analysis of the ChiG and ChiC structures Despite the deletion of the B-loop, the two putative catalytic residues Glu68 and ... to His67 ⁄ Glu182 and Asp184 in molecule A and His67 ⁄ Glu182 and Asp137 in chain B, originally modeled as waters, were replaced by zinc ions, based on the refined B-values and residual peak heights...
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Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
Ngày tải lên :
08/03/2014, 10:20
... described in the Materials and methods phenolic compounds and alginates, etc and hence preserved the enzymatic activity of lamjapin But the efficiency of extraction was still low and the proteins could ... Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate J Biochem Biophys Methods 11, 13–20 Neuhoff, V., Stamm R & Eibl, H (1985) Clear background and highly ... MgCl2) at 35 °C for 90 and then treated with acid aniline The RNA fragments produced by lamjapin and aniline treatment were extracted with phenol/chloroform and precipitated by ethanol After separating...
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Báo cáo Y học: Fluorescent analogs of UDP-glucose and their use in characterizing substrate binding by toxin A from Clostridium difficile pdf
Ngày tải lên :
08/03/2014, 22:20
... studies were performed by varying the MUG and UDP-Glc concentrations and following the kinetics of the hydrolysis reaction Synthesis of MUG, a triethylammonium salt MATERIALS AND METHODS Unless otherwise ... equilibria and their dissociation constants involved in the binding of UDP-Glc, MUG and Mg2+ to TcdA Values shown in parentheses have been obtained by using the thermodynamic cycle and the equilibrium ... supported by the Indiana University College of Arts and Sciences and a grant to A L F from the American Chemical Society-Petroleum Research Fund (#35517-G4) A K was a summer REU student supported by...
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Báo cáo khoa học: Acute intermittent porphyria – impact of mutations found in the hydroxymethylbilane synthase gene on biochemical and enzymatic protein properties pdf
Ngày tải lên :
23/03/2014, 04:21
... presentation is abdominal pain caused by neurovisceral crises [4] Individuals differ from each other with respect to their biochemical and clinical manifestations, and approximately 90% of AIP carriers ... species have been studied and their enzymatic and kinetic properties have been described [12,13] The crystallographic structure of HMBS from Escherichia coli [14,15] and human [16] has been determined ... attack symptoms and asymptomatic carriers or asymptomatic carriers and healthy individuals can have similar measured values of porphyrins and their precursors [18] Together with biochemical diagnoses,...
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Báo cáo khóa học: Overexpression and enzymatic characterization of Neisseria gonorrhoeae penicillin-binding protein 4 ppt
Ngày tải lên :
23/03/2014, 12:20
... EC0 and ECU represent the amount of acyl-enzyme complex formed in the absence and presence of the unlabeled antibiotic, respectively, and CU and CL are the concentrations of the unlabeled and ... inhibitors, and pH dependence Comparison of the enzymatic properties of NG PBP with other DD-carboxypeptidases highlights both similarities and differences within these enzymes, and suggests ... similarities between LMM and HMM PBPs in terms of active site sequence [3] and architecture [14,17,18] Both groups of enzymes also act on cell wall peptidoglycan as their substrate, and are reactive...
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Báo cáo khoa học: X-ray crystallographic and enzymatic analyses of shikimate dehydrogenase from Staphylococcus epidermidis pot
Ngày tải lên :
30/03/2014, 02:20
... formed by a central six-strand mixed b sheet (b2, b1, b3, b5, b6 and b4; b5 is antiparallel to the others) flanked by three a-helices (a1, a9 and a8) on the inner side and by two a-helices (a2 and ... mostly parallel six-stranded b-sheet (b9, b8, b7, b10, b11 and b12) at the core of the domain is flanked by three helices (a4, a5 and g4) on the inner side and by two helices (g5 and a6) on the outer ... induced by NADP binding and stabilized by shikimate binding In summary, we determined the crystal structures and enzymatic properties of SeSDH The combination of the crystallographic and enzymatic...
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DIABETES – DAMAGES AND TREATMENTS doc
Ngày tải lên :
27/06/2014, 18:20
... approach and Hypoglycemia in children It includes some treatment methods and their applications Section B, which includes chapters 3-4, includes a contemporary review about Brittle Diabetes and Hypoglycemia ... lactate and free fatty acids), insulin and counter-regulatory hormones (mainly cortisol and growth hormone) is crucial (Table 5) The result of fasting test may be affected by β-blockers treatment and ... children and is associated with increased mortality rates in critically ill nondiabetic children (Faustino and Bogue, Cornblath and Ichord, 2000, Reid and Losek, 2005) A recent study by Faustino...
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DIABETES – DAMAGES AND TREATMENTS docx
Ngày tải lên :
28/06/2014, 03:20
... approach and Hypoglycemia in children It includes some treatment methods and their applications Section B, which includes chapters 3-4, includes a contemporary review about Brittle Diabetes and Hypoglycemia ... lactate and free fatty acids), insulin and counter-regulatory hormones (mainly cortisol and growth hormone) is crucial (Table 5) The result of fasting test may be affected by β-blockers treatment and ... children and is associated with increased mortality rates in critically ill nondiabetic children (Faustino and Bogue, Cornblath and Ichord, 2000, Reid and Losek, 2005) A recent study by Faustino...
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Báo cáo khoa học: "Polyphenolic and enzymatic characterization of ageing and rejuvenation of hybrid walnut trees (Juglans nigra x Juglans regia): relationship to growth" ppt
Ngày tải lên :
09/08/2014, 02:21
... rejuvenation and their enzymatic regulation (PAL and chalcone synthetase) remains a high priority Discussion It will also be necessary to specify relationships nolic compounds, growth and between ... and synchronous increase of these enzyme activities Results Phenolic ammonia compounds and phenylalanine lyase (PAL) Results on polyphenols are based on main compounds (A and B), elucidated by ... compounds, growth and between typical phe- their metabolism, rooting Ageing and rejuvenation of walnut trees has been characterized by different biochemical factors In addition, studies undertaken...
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Báo cáo y học: "Adaptation of Rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics" docx
Ngày tải lên :
09/08/2014, 23:20
... by endocytosis and surrounded by a plant membrane which controls exchange of carbon and nitrogen between the plant cytosol and rhizobia [4] Despite detailed knowledge of root hair infection and ... leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics Vinoy K Ramachandran1, Alison K East2, Ramakrishnan Karunakaran2, J Allan Downie2 and Philip S Poole2* ... exciting and suggests there may be a wealth of plant rhizosphere-specific plasmids or chromosomal islands to be revealed by emerging high-throughput sequencing projects Material and methods 20...
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báo cáo khoa học: " Origins of the amphiploid species Brassica napus L. investigated by chloroplast and nuclear molecular markers" pps
Ngày tải lên :
12/08/2014, 03:21
... 10) and B oleracea and related wild species (C genome, n = 9) Recreation of stable B napus by crossing B rapa and B oleracea is difficult, but possible Re-synthesized B napus may be generated by ... of their respective progenitors (as originally proposed by [1]) B juncea shares haplotype A:05 with B rapa, whilst B carinata and B nigra share haplotype B:01 Three haplotypes (A:01, A:06 and ... test exactly the same accession used by Song and Osborn as no further seed was available A further 'B montana' accession (not the same as that used by Song and Osborn) was originally included...
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Báo cáo y học: " TRIM5α and TRIMCyp form apparent hexamers and their multimeric state is not affected by exposure to restriction-sensitive viruses or by treatment with pharmacological inhibitors" doc
Ngày tải lên :
12/08/2014, 23:22
... presence of MG132 30% and 10% of the cells were infected (GFP-positive), respectively, by B-MLV-GFP and NMLV-GFP, as seen by flow cytometry days later possible that the nature and number of some ... hexamers and higher-order multimers (Fig 4B, upper panel) TRIMCyp was found as dimers, trimers, and hexamers (Fig 4B, lower panel) An additional band migrating faster than the hexamer was visible and ... stabilized by treatment with glutaraldehyde as first described by Mische and collaborators [23] Surprisingly, TRIM5αrh was not present as a trimer in these cells Rather, we observed a band with...
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Báo cáo y học: "Biophysical and enzymatic properties of the simian and prototype foamy virus reverse transcriptases" pptx
Ngày tải lên :
12/08/2014, 23:22
... conceived and coordinated the study MJH and FM performed the experiments, AR provided reagents and participated in designing the experiments BMW and MJH wrote the paper All authors read and approved ... PFV and SFVmac PR-RT by circular dichroism (CD) spectroscopy The shape of the CD spectra obtained for the two enzymes was highly similar, implying comparable ratios of a-helices and b-strands ... cases, the curves showed a broad minimum between 205 nm and 222 nm, characteristic for a mixture of a-helical and b-strand structures, and high ellipticity near 200 nm Thus, the spectra are indicative...
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Báo cáo y học: "Evaluation of the effects of a VEGFR-2 inhibitor compound on alanine aminotransferase gene expression and enzymatic activity in the rat liver" pdf
Ngày tải lên :
13/08/2014, 13:20
... ALT, ALP and AST enzymatic activity and other biochemical tests were performed with a Hitachi 911 chemical analyzer using a standardized method A necropsy was conducted on each rat on day and gross ... buffered formalin and submitted to histology for processing and staining with H&E, caspase and TUNEL method RNA isolation and reverse transcription Tissues were homogenized by an ultra turrax ... approved by the American Association for the Accreditation of Laboratory Animal Care All protocols were approved by the Pfizer Global Research and Development Institutional Animal Care and Use...
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