d— biliary epithelial and endothelial cells as immunological targets
Báo cáo y học: "The role of the purinergic P2X7 receptor in inflammation" pot
Ngày tải lên :
11/08/2014, 08:21
... Upon release, IL-1β is known to elicit diverse responses, including the activation of macrophages, T -cells and signalling cascades, as well as the induction of cyclooxygenase type (COX-2) and fever ... parasites, and function in the pathogenesis of allergic and immunological disease In general, granulocytes are recruited to sites of inflammation where they release inflammatory mediators such as ... human cells has been demonstrated on PMN, HL-60 promyelocytes and granulocytic differentiated cells, and is reported to increase with granulocytic differentiation [100] However, one report has suggested...
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Báo cáo y học: " Angiotensin-converting enzyme 2 autoantibodies: further evidence for a role of the renin– angiotensin system in inflammation" potx
Ngày tải lên :
12/08/2014, 14:22
... expression was increased and subsequent AT1 receptor blockade or ACE inhibition ameliorated the autoimmune inflammation [7] The present findings by Takahashi and colleagues reveal increased expression ... Pre-eclampsia is associated with circulating autoantibodies against the AT1 protein that act as functional receptor agonists to promote vasoconstriction and inflammation [5] Studies by Harrison and colleagues ... may increase basal ACE2 expression potentially contributing to the protective mechanisms of these therapies There is increasing evidence for the interplay of the renin–angiotensin system and inflammatory...
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Báo cáo y học: " Role of the Fas/FasL Pathway in HIV or SIV Disease" ppsx
Ngày tải lên :
12/08/2014, 23:22
... implicates the Fas death pathway since activated T or B cells express Fas Acute SIV infection triggers rapid increases in Fas and FasL expression in peripheral blood [50] as well as in thymus and other ... described as "chronic immune activation" or "hyperactivation" occurs during HIV disease and is accompanied by higher expression of TNF superfamily ligands and their receptors, e g Fas/FasL and TRAIL-DR5 ... anti-FasL treatment preserved memory T cells, antigen responses after SIV infection, and was associated with decreased levels of Treg cells [65] In the two interventional studies, anti-FasL delivered...
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Báo cáo y học: " Role of the C-terminal domain of the HIV-1 glycoprotein in cell-to-cell viral transmission between T lymphocytes" potx
Ngày tải lên :
12/08/2014, 23:23
... virusassociated p24 per 106 cells) The number of infected cells, as determined by intracellular p24 FACS, was in the range of 10-20% and was adjusted to 10% with unifected cells and then mixed and ... in the cases of Jurkat (np) and CEM-SS (np) cells, the observed decreases were as pronounced as in H9 (np) cells Increased Gag expression in permissive cells presumably leads to increased virus ... (triangles) in MT-4 cells (p) and H9 cells (np) below Cells were infected with equal amounts of virus (equivalent to 100 ng p24 per 106 cells) and washed at h p.i Newly produced virions released into the...
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Báo cáo y học: "Role of the long cytoplasmic domain of the SIV Env glycoprotein in early and late stages of infection" ppt
Ngày tải lên :
13/08/2014, 05:22
... in dividing and nondividing cells we used an indicator cell line assay with human epithelial HeLa cells expressing CCR5 and CD4 The nuclear activation of a galactosidase indicator assay does not ... disease resistant primates in Africa or disease sensitive primates in Asia may indicate that this form of virus appeared when virus is adapting to new cells such as such as epithelial on brain cells, ... produced in monkey PBMC and release of particles was inhibited in human T cells and macrophages In contrast, SIV with truncated Env produced infectious particles in all types of cells tested Effects...
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The Role of Genetically Modified Organisms (GMOs) in Beverage Production
Ngày tải lên :
25/10/2013, 21:20
... PLANTS AND ANIMALS People have been genetically modifying the food supply during the thousands of years since the domestication of plants and animals began Classical breeding and selection, as well ... well as techniques such as radiation breeding, embryo rescue, and transposon mutagenesis, create signiÞcant changes in the genetic makeup of plants and animals due to the random recombination and ... corn, cotton, potato, and tomato; herbicide-tolerant soybeans, corn, rice, sugar beet, ßax, and canola; and virus-resistant squash, papaya, and potato Advantages of insect- and virus-resistant...
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Tài liệu Báo cáo khoa học: The central role of CDE/CHR promoter elements in the regulation of cell cycle-dependent gene transcription pdf
Ngày tải lên :
16/02/2014, 09:20
... CDE ⁄ CHR tandem site lead to a recruitment of different proteins? Are class I and class II promoters bound by different protein complexes? Why are some promoters class I in mouse and class II in ... Muller and K Engeland ¨ Table Criteria for promoters controlled by CDE ⁄ CHR sites Class I Genes not expressed in G0 and G1 cells Genes encode proteins with functions in S, G2 or M phases CHR ... repression In contrast, class II genes not have a functional CDE and are only regulated through a wellconserved CHR Interestingly, some ortholog genes from mouse and human, such as cyclin B2 and Cdc25C,...
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Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
Ngày tải lên :
21/02/2014, 15:20
... 1338 cm)1ặM)1 was measured as described previously [4] For glutaminase activity, a continuous coupled assay was used where the glutamate produced by CTP synthase was oxidized by GDH and monitored ... of glutaminase activity and CTP synthesis at 0.1 mM each of UTP and ATP Spectrophotometrical measurement of CTP synthesis (squares) and glutaminase activity (circles) was performed as described ... the ITC assay was used, the basal glutaminase activity was therefore calculated for the experimental conditions used for GTP activation on the basis of the kinetic constants in Table 1, and those...
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Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
Ngày tải lên :
06/03/2014, 11:20
... pro-tyrosinase Apo pro-tyrosinase Holo trypsinized pro-tyrosinase Apo tyrpsinized pro-tyrosinase Holo core tyrosinase Apo core tyrosinase Holo pro-tyrosinase F453A Apo pro-tyrosinase F453A 2.2 ... tyrosinase has a similar role in forming an alkane thiol bond, as has been shown for the sweet potato catechol oxidase [8] or the N crassa tyrosinase enzyme [15]; therefore, this residue was mutated ... pro-tyrosinase, a major increase in activity upon removal of the C-terminal extension by trypsin was found, namely an approximately 50-fold increase in mono- and a 100-fold increase in diphenolase activitiy...
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The Role of Small and Large Businesses in Economic Development doc
Ngày tải lên :
06/03/2014, 19:20
... some industries and decreases in others, as R&D outcomes, such as patents (Scherer; Acs and Audretsch; Pavitt and others) Thus, a general statement about the relationship between R&D and firm size ... Science and Engineering? NSF 99-322, Project Officers: John Tsapogas and Lawrence M Rausch; Mary Collins, Westat, Arlington, Va Oi, W.Y., and T.L Idson 1999 “Firm Size and Wages,” in O Ashenfelter and ... opportunities; built and maintained high-quality public infrastructure; created a business climate with reasonable levels of taxation and regulation; and, through good government and quality amenities,...
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Báo cáo khoa học: The role of evolutionarily conserved hydrophobic contacts in the quaternary structure stability of Escherichia coli serine hydroxymethyltransferase pptx
Ngày tải lên :
07/03/2014, 03:20
... (2) Activity assays All assays were carried out at 20 °C in 50 mm NaHepes (pH 7.2), containing 200 lm dithiothreitol and 100 lm EDTA The serine hydroxymethyltransferase activity was measured with ... samples with l-serine and H4PteGlu as substrates, as previously described [9] To determine the Km for l-serine, H4PteGlu was maintained at 0.23 mm and the l-serine concentration was varied in the ... (250–310 nm) and visible (310–500 nm) CD spectra were measured using 0.2 and cm path length quartz cuvettes and the results obtained were expressed as the mean residue ellipticity (Q), assuming a...
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Báo cáo khoa học: Use of lithium and SB-415286 to explore the role of glycogen synthase kinase-3 in the regulation of glucose transport and glycogen synthase pdf
Ngày tải lên :
08/03/2014, 08:20
... utilize Li as an inhibitor of GSK3 is that the ion also affects the activity of a number of other molecules such as casein kinase-2 and mitogen activated protein kinase (MAPK)-2 [23] as well as enzymes ... of 10 lM cytochalasin B Radioactive medium was aspirated prior to washing adherent cells three times with 0.9% (w/v) ice-cold saline Cells were subsequently lysed in 50 mM NaOH and radioactivity ... in cell lysates was determined using the Bradford reagent as described previously [32] Glycogen synthase The activity of GS was assayed as described previously [31] Briefly, assay buffer (67 mM...
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The Role of Gestures and Facial Cues in Second Language Listening Comprehension pptx
Ngày tải lên :
10/03/2014, 05:20
... listening comprehension task, and completion of the questionnaire The questionnaire was completed after the listening task so as not to bias any of the responses Results and Discussion To give ... lecturer emphasized important information with constant movements of her hands or emphasized a key term with one hand movement associated with a higher pitch and greater stress, as in (7), in ... about the listening task Procedure Listening comprehension task The experiment was conducted in a regular classroom equipped with a built-in computer and a speaker that was suspended from the...
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Báo cáo khoa học: The role of the Fe-S cluster in the sensory domain of nitrogenase transcriptional activator VnfA from Azotobacter vinelandii potx
Ngày tải lên :
15/03/2014, 09:20
... curve was constructed using BSA as a standard Quantification of acid labile sulfur was carried out as described previously [55], and inductively coupled plasma–optical emission spectroscopy was employed ... temperature was lowered to 20 °C, and then IPTG was added to a final concentration of 0.5 mm and the culture was incubated for 16 h The cells were harvested by centrifugation (6000 g for at °C) and the ... vinelandii contains three distinct nitrogenases Nitrogenase-1 is a conventional molybdenum nitrogenase that bears a metal-sulfur cluster with molybdenum and iron as the reactive site By contrast,...
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Bringing the Hidden Giants to the Footlight: the Role of Savings and Retail Banks in Increasing the Level of Access to Financial Services docx
Ngày tải lên :
15/03/2014, 10:20
... countries such as Thailand, where WSBI member GSB (Government Savings Bank) has a “School Based Savings Programme” in which students recreate a savings bank in their class and acquire the basic principles ... Thailand has been very active in implementing policies and measures to alleviate and eradicate poverty in the country Individually and in collaboration with other financial institutions and government ... capacity and is not based on collaterals Personal references and overall household expenses are part of the credit analysis FEATURE ARTICLES People’s Bank Program: This was established by GSB to expand...
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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx
Ngày tải lên :
16/03/2014, 10:20
... was performed Fig (A) Molecular mass determination of apo-ACP and holo-ACP Molecular masses of apo-ACP and holo-ACP were determined with an Ultra Flex TOF MALDI-TOF mass spectrometer (a) Mass ... synthesis assay with holo-ACP E coli acyl-ACP synthase (AAS) utilizes holo-ACP as a substrate and converts it to acyl-ACP E coli AAS also utilizes fatty acids with various chain lengths as substrates, ... Lysozyme (2 mg) was added, and the mixture was incubated on ice for 30 Cells were disrupted using a probe-type ultrasonicator (Vibra-Cell; Sonics and Materials, Newtown, CT, USA) MgCl2 and MnCl2 were...
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Báo cáo Y học: Elucidation of the role of fructose 2,6-bisphosphate in the regulation of glucose fluxes in mice usingin vivo 13 C NMR measurements of hepatic carbohydrate metabolism docx
Ngày tải lên :
17/03/2014, 17:20
... balancing the uptake and release of glucose from the liver [8,9] 13 C NMR spectroscopy has been used to measure glucose and glycogen metabolism in respiring isolated liver cells [12,13] and perfused ... incorporation into glycogen C1 and C6, as well as changes in total glycogen content in vivo, which can be used to assess the activity of the direct and indirect pathway, as well as net glycogen changes ... Vsyn, Vphos and Vnet represent the flux through glycogen synthase, phosphorylase, and the rate of net glycogen synthesis, respectively 13Glyc1 and 13Glyc6 represent 13Clabeled glycogen C1 and C6 concentration,...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
Ngày tải lên :
17/03/2014, 23:20
... significantly increased affinity for synthetic acyl donors and with increased potential for transferase reactions MATERIALS AND METHODS Strain and plasmids Mutants were constructed using the plasmid pEC ... high amidase activity encountered in PA are not known The wild-type has a higher esterase/amidase ratio for phenylacetylated substrates than bF24A, whereas bF24A has a higher esterase/amidase ratio ... out according to standard protocols [8] Purifying PE and enzymes Isolation of periplasmic extracts and purification of the enzymes was carried out as described [7] Kinetic measurements with bF24L,...
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The role of floating-rate bank loans in institutional portfolios pdf
Ngày tải lên :
22/03/2014, 17:20
... risks such as interest rate, credit, repurchase and reverse repurchase transaction risks Greater risk, such as increased volatility, limited liquidity, prepayment, non-payment and increased default ... opportunities in other fixed income asset classes Conclusion As an asset class, we believe bank loans are likely to outperform most other fixed income asset classes that have duration risk in a ... with other fixed income asset classes, but that as diversifiers they are not as strong as the Barclays Capital U.S Aggregate Bond Index relative to equity-linked asset classes (see Exhibit 2a)...
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