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Mechanisms in dendrite pruning of drosophila dendritic arborization neurons
Ngày tải lên :
14/09/2015, 08:38
... vii List of Publications viii List of Tables ix List of Figures x List of Abbreviations xii Chapter One: Literature Review ... occurring neuronal remodeling Besides the mammalian models mentioned earlier, the Drosophila nervous system has also proven to be an appealing model to study neuronal remodeling with higher resolution ... takes the form of a worm-like creature, only capable of feeding and crawling; however, when the animal reaches its adult stage, it develops the ability of flying and looks like a ‘fruit fly’ as...
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Báo cáo khoa học: b-Secretase cleavage is not required for generation of the intracellular C-terminal domain of the amyloid precursor family of proteins pot
Ngày tải lên :
15/03/2014, 10:20
... proteins contained one intronspanning region to avoid amplification of genomic DNA Quantitative real-time PCR reactions were run in duplicate One microliter of Taq DNA polymerase and the appropriate primer ... APPsa [(B) and (D), respectively] were quantitated by densitometry, and values normalized versus WT control are presented as averages ± standard errors of duplicate measurements of three animals ... [(B) and (D), respectively] were quantitated by densitometry, and values normalized relative to WT control are presented as averages ± standard errors of duplicate measurements of three animals...
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Báo cáo hóa học: " Human cytomegalovirus UL27 is not required for viral replication in human tissue implanted in SCID mice" ppt
Ngày tải lên :
20/06/2014, 01:20
... viruses replicate to a slightly lesser degree than the parent viruses as evidenced by the uniform absence of replication one week following inoculation This is also consistent with the slightly ... type wt 12 multiplicity of infection MOI 13 Sia IG, Patel R: New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients Clin Microbiol ... Acknowledgements The authors thank Caroll Hartline and for titering virus samples, and Deb Collins for expert technical assistance These studies were supported by Public Health Service contract NO1-AI-30049...
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Báo cáo hóa học: " Membrane-associated heparan sulfate is not required for rAAV-2 infection of human respiratory epithelia" pot
Ngày tải lên :
20/06/2014, 01:20
... HSPG from cell membranes, human tracheal epithelial HTE cell line, fetal human tracheal epithelial FHTE cell line, and cystic fibrosis IB3-1 cell line were treated with increasing amounts of heparinase ... observed in respiratory epithelial cell lines was not noted in the CHO cell lines, suggesting that this high MOI effect is either unique to respiratory epithelial cell lines or was caused by a small ... (Falmouth, MA) Cell culture Immortalized human cell lines Human Tracheal Epithelial (HTE), Fetal Human Tracheal Epithelial (FHTE), and cystic fibrosis bronchial epithelial (IB3-1) [26] were cultured...
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Báo cáo y học: "Wildtype epidermal growth factor receptor (Egfr) is not required for daily locomotor or masking behavior in mice" pps
Ngày tải lên :
10/08/2014, 09:20
... h light:dark cycles and three-hour light pulses given during the dark cycle Locomotor Locomotor activity in Egfrwa2/wa2 mice during 12 h:12 h light:dark cycles and three-hour light pulses given ... the light phase of the cycle For the masking response analysis, either one or three hour light pulses were delivered on the third day starting at Zeitgeber Time (ZT) 14 (ZT0 = light on; ZT12 = lights ... modifiers given the small number of individuals tested Previous studies utilizing direct infusion of ligand to hyperactivate EGFR in the brain have shown that abnormally high EGFR signaling is capable...
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Báo cáo Y học: The cytoplasmic C-terminus of the sulfonylurea receptor is important for KATP channel function but is not key for complex assembly or trafficking pdf
Ngày tải lên :
31/03/2014, 08:20
... SUR1del145myc (line C1) and Kir6.2mycHis6 + SUR1del146myc (line C2), Kir6.2 + SUR1del196myc (line D) and Kir6.2 + SUR1del249myc (line E) with G418 and Zeocin as previously described [15,18] Lines were ... bath applied diazoxide was determined in the whole-cell configuration The application of 300 lM diazoxide induced large whole-cell currents in line A but led to little increase in the other lines ... stable line) Data analysis Radioligand binding experiments were fitted by the binding isotherm y ¼ BmaxÆx/(x + Kd) where y is the bound specific radioligand (pmolÆmg protein)1) and x is the radioligand...
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Báo cáo y học: "JNK1 is not essential for TNF-mediated joint disease" doc
Ngày tải lên :
09/08/2014, 06:22
... brief, joint swelling was assessed using a clinical score graded from to (0, no swelling; 1, mild swelling of toes and ankle; 2, moderate swelling of toes and ankle; 3, severe swelling of toes and ... [19] Like other MAPK pathways, JNK signalling is activated in the synovial tissue of RA patients [12,20-22] Studies with cultured synovial fibroblast-like cells from RA patients have established ... using a 700-W microwave oven Tissue sections were cooled to room temperature and then rinsed using detergent solution: 0.5% Tween in phosphate-buffered saline (PBS) For quantification of inflammation,...
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Báo cáo y học: "Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 " ppt
Ngày tải lên :
13/08/2014, 05:21
... severe late-phase limitations described in rodent cell lines may thus not necessarily be predictive for the ability of primary cells to support these steps of the HIV-1 replication cycle In the ... HF, Peterlin BM: Human p32 protein relieves a post-transcriptional block to HIV replication in murine cells Nat Cell Biol 2003, 5(7):611-618 Swanson CM, Puffer BA, Ahmad KM, Doms RW, Malim MH: ... permissive for HIV replication through a block-by-block humanization (for an overview [11]) This conceptual approach seeks to surmount intrinsic limitations in the HIV-1 replication cycle in small...
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Báo cáo y học: " The carbohydrate at asparagine 386 on HIV-1 gp120 is not essential for protein folding and function but is involved in immune evasion" potx
Ngày tải lên :
13/08/2014, 06:20
... reporter cell line for the detection and quantitation of a single cycle of HIV and SIV replication Virol 2000, 273:307-315 Baribaud F, Pohlmann S, Leslie G, Mortari F, Doms RW: Quantitative expression ... with immunogenicity, removal of the 386 glycan in trimeric Env vaccines may facilitate elicitation of neutralizing b12-like CD4BS antibodies Studies to test this hypothesis are in progress Methods ... neutralization experiments and FG performed the DC-transmission experiments IljaB, DE, MM, EB and MMD carried out the cloning, virus replication experiments, neutralization experiments and gp120 ELISAs...
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Báo cáo y học: "Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue" docx
Ngày tải lên :
12/08/2014, 23:22
... mutation in Vpu impairs the ability of HIV-1 to replicate in macrophages, but not in ex vivo infected HLT cultures or PBL This difference is most likely due to a reduced capability in counteracting tetherin, ... calculated with the linear regression module Additional file 1: Supplementary Figure S1 Assessment of viral release by quantitative WB correlates with p24 ELISA Correlation of the quantitative WB ... attenuate HIV-1 replication (Fig 4A and 4B) Consistent with the results of Figure Vpu S52A is dispensable for HIV-1 replication and cytopathicity in ex vivo infected HLT Representative replication kinetics...
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Báo cáo y học: " Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2" ppsx
Ngày tải lên :
12/08/2014, 23:23
... either the medial Golgi localized Nacetylglucosaminyltransferase I (CHO Lec1) or the trans Golgi localized CMP-sialic acid transporter (CHO Lec2) have lost their abilities to produce complex N-glycans ... 6:859-868 16 Geijtenbeek TB, Kwon DS, Torensma R, Van Vliet, van SJ, Duijnhoven GC, Middel J, Cornelissen IL, Nottet HS, KewalRamani VN, Littman DR, Figdor CG, van Kooyk Y: DC-SIGN, a dendritic ... pBRNL4-3-EGFP in which nef was replaced by EGFP Oligonucleotide sequences (env sequences in bold; EGFP sequences in italics, MluI restriction site underlined): pJM206 5'TGGAACTTCTGGGACGCAGG-3'; pJM394...
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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf
Ngày tải lên :
20/02/2014, 01:20
... and analyzed in a Phosphorimager (Molecular Dynamics, Sunnyvale, CA, USA) Metabolic labeling of rRNA Metabolic labeling was performed as described previously [9] Exponentially growing cultures ... and RFP–Nop1p fusion proteins Living cells were immobilized on l-polylysine coated histological slides, in aqueous medium The preparations were covered with cover slips, sealed and immediately ... after [3H]uracil labeling (B) Analysis on agarose gel of pre-rRNA labeled with [methyl-3H]methionine (C) Total RNA separated on polyacrylamide gel after [3H]uracil labeling An aliquot of 20 lg of...
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Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
Ngày tải lên :
22/02/2014, 07:20
... the utilization of the first cistron increased only 2.2–2.7-fold whereas a 6–7.9-fold increase in utilization of the IRESmediated second cistron was observed This was in contrast with utilization ... has been implicated in cellular growth control, as well as in differentiation and apoptosis (reviewed in [36–39]) It seems likely that the regulatory function of eIF2 depends on the delicate balance ... cellular milieu [11] More specifically, the PDGF2 mRNA leader was shown to mediate efficient translation under conditions of megakaryocytic differentiation which provide a permissive milieu for activation...
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Báo cáo khoa học: YidC is required for the assembly of the MscL homopentameric pore potx
Ngày tải lên :
07/03/2014, 02:20
... probably by facilitating partitioning of hydrophobic transmembrane segments from the Sec translocon into the lipid bilayer YidC has also been implicated in the folding and quality control of ... of the lipid bilayer In contrast, position 68, which was used in the earlier study [13], is located adjacent to the centre of the pore-forming TM1 It is therefore conceivable that even a slight ... suspensions were divided into three aliquots, and 10% toluene and 0.2% sodium deoxycholate were added to one aliquot to disrupt the cells The aliquots were equilibrated at 30°C for 10 Subsequently,...
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Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx
Ngày tải lên :
07/03/2014, 05:20
... phospholipase C activity in a coupled enzyme assay with phosphatidylcholine as the substrate In this assay, phospholipase C converts phosphatidylcholine to phosphocholine and diacylglycerol Alkaline ... lipase (Triacylglycerol) lipase (Pancreas) lipase PLC PLD PLA PLA Activity parameters (pmolÆh)1Ælg)1) KM 6.3 lM; Vmax 0.17 lmolÆs)1 5.6 ± 1.5 2.0 ± 0.1 Below detection limit Below detection limit ... a membrane-active lipase that contributes to metabolism or the membrane shaping of peroxisomes Peroxisomes are sites of lipid metabolism It is thus not surprising to find a lipase associated with...
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Báo cáo khoa học: Caspase-8- and JNK-dependent AP-1 activation is required for Fas ligand-induced IL-8 production ppt
Ngày tải lên :
07/03/2014, 09:20
... untransformed human embryonic kidney cell line, HEK293 Conveniently, using this cell line we can exclude the side-effect of cell death, because this cell line does not show any detectable apoptosis ... complementing generalized lymphoproliferative disease (lprcg)-type point mutation (Val238 to Asn) in the DD (FP1), which abolishes Fas’s apoptosis-inducing capacity [40], also abrogated its ability to activate ... compilation ª 2007 FEBS 2383 Mechanisms of Fas ligand-induced AP-1 activation N Matsumoto et al 36 Tang G, Minemoto Y, Dibling B, Purcell NH, Li Z, Karin M & Lin A (2001) Inhibition of JNK activation...
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Báo cáo khoa học: Catalytically active membrane-distal phosphatase domain of receptor protein-tyrosine phosphatase a is required for Src activation doc
Ngày tải lên :
15/03/2014, 10:20
... anti-pY416 and anti-Src Igs, as indicated Src phosphorylation was quantified and the values were normalized for the total amounts of Src The quantification results are presented under the corresponding ... knockout mice are indistinguishable from wild-type siblings [40,41], corroborating the conclusion that a single RPTPa allele is sufficient for mammalian life Mutant RPTPa-D2-R554H was almost completely ... Debant A & Streuli M (1995) The LAR transmembrane protein tyrosine phosphatase and a coiled-coil LARinteracting protein co-localize at focal adhesions EMBO J 14, 2827–2838 Wang Y, Liang L & Esselman...
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Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
Ngày tải lên :
16/03/2014, 11:20
... recently published structural model of higher-plant PSI based ˚ on a crystal structure at 4.4 A does not reveal the presence of PSI-N [10], and cross-linking experiments have shown little interaction ... plants In total, 44 full-length PSI-J sequences were aligned using CLUSTAL W In the alignment shown are the sequences from plants [Arabidopsis thaliana (ARATH) and Nicotiana tabacum (TOBAC)], algae ... plants, P700 oxidation was almost linearly related to increasing light intensity However, in the DPSI-J plants the redox state of P700 was higher than wild-type at all light intensities (Fig 4) This...
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Báo cáo khoa học: Multi-tasking of nonstructural gene products is required for bean yellow dwarf geminivirus transcriptional regulation potx
Ngày tải lên :
16/03/2014, 13:20
... bombarded with pBYD–LIR or pBYD–LIRSIR and either pBYSK1.4 or p35SRep (Fig 1) A Southern blot was performed to demonstrate that pBYD–LIR was replication-incompetent (Fig 2A) Replication products ... V1 ORF and religated with T4 ligase (New England Biolabs) pBYV1X was generated by SalI digestion, blunt-ended with mung bean exonuclease (New England Biolabs) and religated with T4 ligase (New ... K L Hefferon et al BeYDV, like other geminiviruses, replicates via a rolling circle mechanism First, the host cell replication machinery synthesizes a complementary sense-strand...
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