cytotoxic protective activity of nitric oxide in cancer

... the increased NO production of lupus monocytes [31] NO regulates mammalian target of rapamycin activity and TCRζ expression in SLE The mammalian target of rapamycin (mTOR) is a serine/ threonine ... Triptolide, an active component of the Chinese herbal remedy Tripterygium wilfordii Hook F, inhibits production of nitric oxide by decreasing inducible nitric oxide synthase gene transcription ... to be independent of SLE disease activity [37,38] Sequencing of genomic DNA and TCRζ transcripts showed mutations in the coding region of TCRζ from lupus T cells [39] There is a direct interaction...

Ngày tải lên: 12/08/2014, 14:22

... healing 57 1.5.3.1 Nitric Oxide donors previously studied in wound healing 1.5.3.2 Nitric Oxide inhibitors previously studied in wound healing 59 1.5.3.3 Nitrosothiols 60 1.5.3.4 Interaction of Nitric ... expression of Nitric oxide synthase in normal human skin and Keloid: Effect of Steroids on the expression of NO in keloids In: Wound Healing Society Conference, Institute of health, Singapore, ... healing- Role Of nitric oxide in the reparative process In: Wound Healing Society Conference, Institute of health, Singapore, Oct 1999 4) Achuth HN, SM Moochhala, WTL Tan Effect of nitric oxide...

Ngày tải lên: 17/09/2015, 17:19

... Nitric oxide mediates interleukin-1 induced inhibition of glycosaminoglycan synthesis in rat articular cartilage Mediators Inflamm 1995, 4:107-111 48 Murrell GAC, Jang D, Williams RJ: Nitric oxide ... and incubated in the presence of the biotin-conjugated secondary antibody for 45 minutes at room temperature followed by the addition of the avidin-biotin-peroxidase complex for 45 minutes All incubations ... findings are in accordance with those of Cake and coworkers [9] in an ovine meniscectomy model of OA Many proteases have been shown to play major roles in the catabolism of OA cartilage For instance,...

Ngày tải lên: 09/08/2014, 14:20

... sonicated in a bath-type sonicator for 5min The amount of protein of the solubilized cells was determined with a bicinchoninic acid protein assay kit (PIERCE, Rockford, IL, USA) using bovine serum ... mode of actions of TS, we examined the effect of TS on LPS/IFNinduced NO production in VSMC We found that TS activated LPS/IFN-induced NO production in VSMC through enhancement of iNOS protein ... induce apoptosis in various cell lines [8–15,29], the inhibitory effect of TS on the cell growth at higher concentrations might prevent the increase in the enhancing effect of TS on LPS/IFN-induced...

Ngày tải lên: 22/02/2014, 04:20

... combination of tighter binding of cadmium than zinc in metallothioneins and the preference of zinc in the more flexible b-domain In addition to the already described differences in flexibility of ... activity [31] This activity relies on the suppression of the expression and activity of inducible nitric oxide synthase (iNOS) by zinc [32,33], released from MT under the in uence of nitric oxide ... from the cytotoxic and DNA-damaging effects of nitric oxide [29] So, while the binding of NO to GSH in vivo does not obviously prevent NO from interacting with MT2, we have shown that in vitro...

Ngày tải lên: 07/03/2014, 15:20

... by anti-IFN-c Ig, of LPS-induced NO production in murine PEC but not in the RAW264.7 murine macrophage cell line Cells of murine PEC and of the RAW264.7 murine macrophage cell line were cultured ... production of NO in macrophages stimulated with LPS, via activation of NF-jB and its binding to region in the promoter of the iNOS gene [11,12] In the present study, LPS-induced NO production in the ... factor IRF-1 in NO synthase induction in macrophages Science 263, 1612–1615 14 Martin, E., Nathan, C & Xie, Q.-W (1994) Role of interferon regulatory factor in induction of nitric oxide synthase...

Ngày tải lên: 17/03/2014, 10:20

... cleavage of inducible nitric oxide synthase (iNOS) by calpain I Biochim Biophys Acta 1568, 216– 224 23 Su Y & Block ER (2000) Role of calpain in hypoxic inhibition of nitric oxide synthase activity in ... previously to induce an increase in the level of [Ca2+]i and a correlated calpain activation [28] This increase in [Ca2+]i is more intense in aorta than in brain Under these conditions, in brain, no ... right amount of nitric oxide Am J Physiol Renal Physiol 285, 178–190 In vivo degradation of NOS and HSP90 by calpain Kone BC (2000) Protein–protein interactions controlling nitric oxide synthases...

Ngày tải lên: 30/03/2014, 04:20

... forebrain cholinergic neurons [57] and direct TNFα administration in the brain of mice results in an impairment of basal forebrain cholinergic function [58] However, whether induction of iNOS and ... TNFα in the presence A: absence blots detecting iNOS in total NGF in A: Western blots detecting iNOS in total protein extracts from PC12 cells treated for 24 hr with increasing concentrations of ... cytokines of the inducible nitric oxide synthase promoter in insulin-producing cells Diabetologia 1998, 41:1101-8 Kong GY, Peng ZC, Costanzo C, Kristensson K, Bentivoglio M: Inducible nitric oxide...

Ngày tải lên: 19/06/2014, 22:20

... iNOS may be involved in the process of apoptosis [40] In conclusion, our results showed that the three isoforms of NOS including nNOS, eNOS, and iNOS were increased in the initiation of EAE and ... forms of nitric oxide synthase in autoimmune encephalomelitis 27 28 29 30 31 32 33 34 35 36 37 38 Induction of nitric oxide synthase activity in human astrocytes by interleukin-1beta and interferon-g ... expression of both nNOS and eNOS in the spinal cords of rats with EAE, small amounts of iNOS were identified in the normal spinal cords but its expression slightly increased in the spinal cord of 5CFAtreated...

Ngày tải lên: 07/08/2014, 14:22

... and inducible (iNOS) isoforms of nitric oxide synthase (NOS) in the ovaries of pigs The intensity of staining is indicated by (-), where staining was absent, up to (+++), for maximal staining ... immunostaining for three isoforms of NOS was detected in the granulosa and theca cells This finding suggests that, in the porcine ovary, granulosa and theca cells may serve as sources of nitric oxide ... The involvement of nitric oxide in the ovulatory process in the rat Endocrinology 1994, 135, 2287-2290 11 Takesue K, Tabata S, Sato F, Hattori MA Expression of nitric oxide synthase-3 in porcine...

Ngày tải lên: 07/08/2014, 18:21

... NOx/creatinine ratios acts as a control for individual differences in creatinine clearances The finding of increased NOx and NOx/creatinine ratios in serum (but not in urine) in patients with RA raised the ... role of nitric oxide in the pathogenesis of spontaneous murine autoimmune disease: increased nitric oxide production and nitric oxide synthase expression in MRL-lpr/lpr mice, and reduction of spontaneous ... shown in our study, individuals vary substantially in their dietary intake of nitrate and nitrite Unless individual variability of NOx intake is taken into account, measures of NOx in urine and...

Ngày tải lên: 09/08/2014, 08:22

... a portion of hTR component containing a telomeric template element using RT-PCR In normal conditions, only inhibition of the template region can lead to the inhibition of telomerase activity we ... information Yingying Lu, Ph.D., Associate professor, Department of Medicine, Beijing Friendship Hospital affiliated to Capital Medical University, Beijing, China 100050 Junchao Gu, Ph.D., Professor, ... 45°C in Figure The telomerase activity Cellular telomerase activity of eukaryotic bel7402-RZ, HCT116-RZ and L02-RZ are shown in table The telomerase activity of bel7402-RZ cells dropped continuously...

Ngày tải lên: 10/08/2014, 10:20

... decrease in iNOS expression in cytosol after inflammatory stimulation, inhibiting superoxide generation and resulting in prevention of tissue injury in patients with chronic airway diseases, including ... concentration of 2.0 μg for examining iNOS mRNA expression Poly A+ mRNA in a volume of 150 μl were added into each well of a 96-well microplate that contained 50 μl of specific probe in duplicate and incubated ... LPS-induced iNOS expression in murine macrophages and hepatocytes [36,37] The protein kinase C (PKC) pathway is also reported to be involved in LPS-induced iNOS expression, and activation of PKC isoforms,...

Ngày tải lên: 11/08/2014, 03:20

... decrease in iNOS expression in cytosol after inflammatory stimulation, inhibiting superoxide generation and resulting in prevention of tissue injury in patients with chronic airway diseases, including ... concentration of 2.0 μg for examining iNOS mRNA expression Poly A+ mRNA in a volume of 150 μl were added into each well of a 96-well microplate that contained 50 μl of specific probe in duplicate and incubated ... LPS-induced iNOS expression in murine macrophages and hepatocytes [36,37] The protein kinase C (PKC) pathway is also reported to be involved in LPS-induced iNOS expression, and activation of PKC isoforms,...

Ngày tải lên: 11/08/2014, 06:22

... ventilatory settings, it is not capable of maintaining a stable inspiratory NO concentration in the face of decelerating inspiratory flow, changing tidal volumes and I/E ratios such as occurs during pressure ... Umamaheswara Rao GS, et al: Distribution of inhaled nitric oxide during sequential and continuous modes of administration into the inspiratory limb of the ventilator Intensive Care Med 1997, 23:849-858 ... suggesting the existence of a bolus of NO moving in front of the tidal volume (b) Nitric oxide is administered from a 900 ppm cylinder The bolus effect is less pronounced than with a 22.5 ppm cylinder...

Ngày tải lên: 12/08/2014, 18:20

... INO was discontinued This response may reflect downregulation of endogenous nitric oxide synthase activity secondary to the administration of exogenous nitric oxide [15] In addition INO may increase ... severe hypoxia as in asphyxia and RDS A number of studies have noted a general lack of a sustained improvement in oxygenation in response to INO in the management of CDH [12–14] In this study ECMO ... none of the infants with CDH responded to INO therapy Inhalation of NO significantly decreased OI (P < 0.0001) and improved the a/A ratio (P < 0.0001) within the first h of INO therapy in 61.1% of...

Ngày tải lên: 12/08/2014, 18:20

... considered This interesting approach was successful in the experimental setting of acute lung injury and needs to be verified in clinical indications other than lung transplantation Competing interests ... surfactant function in ischaemia-reperfusion injury in minipigs Eur Respir J 1999, 13:1037-1043 Strüber M, Harringer W, Ernst M, Morschheuser T, Hein M, Bund M, Haverich A: Inhaled nitric oxide as a prophylactic ... function starts to improve following completion of surgery and is usually best 3–6 months after transplantation In the cases of graft failure, brain death and infection of the donor lung, reduced surfactant...

Ngày tải lên: 12/08/2014, 18:21

... [29] These findings indicate that cNOS inhibition seems to be detrimental, and iNOS inhibition is a possible target of novel therapeutic agents in bronchial asthma Disappointingly, Singh [30] reported ... Guinea pigs weighing 150-200 g were intraperitoneally administered with 2.0 mg of OVA and 100 mg of aluminum hydroxide [Al (OH)3] two days after an intraperitoneal administration of 30 mg/kg cyclophosphamide ... performed the iNOS inhibition experiment using NOS inhibitors, in non-sensitized guinea pigs with normal cough reflex sensitivity and in OVA-sensitized guinea pigs which were of increased cough...

Ngày tải lên: 13/08/2014, 10:20

... reduced and the PaCO2 increased with endotoxin infusion (Table 1) Effect of inhaled nitric oxide Inhalation of NO caused an increase in the PaO2/FiO2 of 50 mmHg (+22% of pre-INO PaO2/FiO2) when ... Lundin S, Mang H, Smithies M, Stenqvist O, Frostell C: Inhalation of nitric oxide in acute lung injury: results of a European multicentre study The European Study Group of Inhaled Nitric Oxide Intensive ... work is part of the project: Influence of endothelin activity on response to inhaled nitric oxide on ventilation perfusion distribution in acute lung injury, which was awarded The Alain Harf Award...

Ngày tải lên: 13/08/2014, 11:23

... Cauwels A: Nitric oxide in shock Kidney Int 2007, 72:557-565 24 Su CF, Yang FL, Chen HI: Inhibition of inducible nitric oxide synthase attenuates acute endotoxin-induced lung injury in rats Clin Exp ... 24 Time after injury (hrs) Figure Time course of (a) incucible nitric oxide synthase (iNOS) mRNA determined by RT-PCR and (b) iNOS protein expression determined by western blotting in lung tissue ... endothelial nitric oxide synthase; FiO2: fraction of inspired oxygen; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; IL: interleukin; iNOS: inducible nitric oxide synthase; nNOS: neuronal nitric oxide...

Ngày tải lên: 13/08/2014, 21:20