cytokine analysis by immunoaf fi nity capillary electrophoresis

... whole organism/system Capillary electrophoresis the method of choice for this analysis is described in the following section 1.4 Capillary Electrophoresis - Basic Principles Electrophoresis can ... (as in gel electrophoresis) or can be a liquid, in most cases a buffer solution In capillary electrophoresis, the medium is contained in a small capillary 40 Therefore capillary electrophoresis ... 41 or separation of a mixture in a capillary tube due to differing ionic mobilities induced by application of a high voltage along the capillary capillary electrophoresis system is shown in figure...

Ngày tải lên: 11/09/2015, 10:14

... 1.1 Electrophoresis 1.2 History of capillary electrophoresis 1.3 Basic principles of capillary electrophoresis 1.4 Different modes of capillary electrophoresis 19 1.5 Instrumentation for capillary ... capillary electrophoresis 24 1.6 Scope of research 32 Part I 35 Analysis of intact bacteria and bacterial DNA Chapter Electrophoretic behavior analysis of intact bacteria by capillary electrophoresis ... CE modes include capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), micellar electrokinetic chromatography (MEKC), capillary electrochromatography (CEC), capillary isoelectric...

Ngày tải lên: 15/09/2015, 17:11

... achieved by manually excluding tandem mass spectra of poor quality, by restricting the database to the proteins that were identified in a first pass approach, and by changing the SEQUEST parameter file ... spectrum that failed to be confidently identified in the first pass analysis were manually interpreted (Table 1) Of the initial 51 tandem mass specta obtained by LC-MS/ MS analysis of the 190 kDa band ... copurified with the immunoprecipitation of BAP31 Identification of proteins separated by one- or two-dimensional SDS/PAGE is usually performed by peptide mass fingerprinting, wherein protein identification...

Ngày tải lên: 20/02/2014, 23:20

... sensitive test of angiotensin I processing by ACE and its inhibition in a onestep analysis by capillary zonal electrophoresis Thus, we report for the first time in invertebrate the existence of ... peptides were analyzed by capillary zonal electrophoresis Samples (2 nL) were injected under vacuum into a PACE 5000 capillary electrophoresis system (Beckman) equipped with a silica capillary (length ... The capillary effluent was monitored by absorption at 214 nm Retention time of each peptide was determined under these migration conditions [23] The quantification of peptides was carried out by capillary...

Ngày tải lên: 23/03/2014, 12:20

... phosphorylated by CK2 We also demonstrate that the phosphorylation of Cdc37 can be analyzed by phospho-af nity gel electrophoresis Together, the phospho-specific antibody and phospho-af nity gel electrophoresis ... analyzed by phospho-af nity gel electrophoresis (A) Non-phosphorylated (lane 1) and phosphorylated (lane 2) Cdc37 were separated by phospho-af nity gel electrophoresis and analyzed by western ... the Cdc37 mobility shift seen in phospho-af nity gel electrophoresis Analysis of Cdc37 phosphorylation and dephosphorylation by phospho-af nity gel electrophoresis To analyze the time course...

Ngày tải lên: 30/03/2014, 03:20

... 250 250 The current study confirms and extends our initial observation using af nity CE [14] by applying two additional biophysical techniques to confirm the specificity of this interaction with ... monoisotopic mass of 1602.78 for AP-1 By amino-acid sequencing using MS, we found that the mass deficit of approximately 18 in the modified peptide corresponds to the first two residues (results not shown) ... Heparin binding specificity of synthetic AP-1 peptides in capillary electrophoresis (CE) A : mixture of AP-1 peptide and the scrambled AP-1 peptide (both 0.5 mgÆmL)1 in water, final concentrations...

Ngày tải lên: 31/03/2014, 23:20

... namely capillary zone electrophoresis (CZE), capillary gel electrophoresis (CGE), isotachophoresis (ITP), isoelectric focusing (IEF), capillary electrokinetic chromatography (EKC) and capillary ... Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis Authored by Peter Mikuš Published by InTech Janeza Trdine 9, 51000 Rijeka, ... quantification, LOD = limit of detection 12 Chiral Capillary Electrophoresis in Current Pharmaceutical and Biomedical Analysis 2.2.1 Capillary electrophoresis The unique properties of CE in terms...

Ngày tải lên: 28/06/2014, 06:20

... were analyzed by IEF at pH 3–10, followed by 12.5% SDS-PAGE and visualized by cCBB-staining Name abbreviations, corresponding to those in Tables and 3, indicate the spots, identified by LC-ESI-MS/MS, ... production by plant nitrate reductase in vivo and in vitro J Exp Bot 2002, 53:103-110 Igamberdiev AU, Bycova NV, Hill RD: Nitric oxide scavenging by barley hemoglobin is facilitated by a monodehydroascorbate ... revealed that 20 spots in roots and 18 spots in leaves were affected by nitrogen availability The analysis of these spots by LC-ESI-MS/MS allowed to identify 15 and 14 proteins in root and leaf...

Ngày tải lên: 12/08/2014, 03:21

... coating capillary wall (hydrophillicity or charge) Stability often problematic Table 1.2 Different Modes of High Performance Capillary Electrophoresis CE Mode Capillary zone electrophoresis (CZE) Capillary ... Introduction 1.1 Principles and historical perspective of Capillary Electrophoresis Since the introduction of capillary electrophoresis (CE) in 1980s by Jorgenson, the good performance and relatively ... conventional slab gel electrophoresis, and produces longer analysis times Performing electrophoresis in a capillary allows the heat to be effectively dissipated through the capillary walls and...

Ngày tải lên: 11/09/2015, 14:27

... Cyclodextrin CE Capillary Electrophoresis CEC Capillary Electrochromatography CF Concentration factor CF-FAB Continuous-flow fast atom bombardment CGE Capillary gel electrophoresis CIEF Capillary isoelectric ... 1.3.3 Capillary Gel Electrophoresis (CGE)………………………… 15~16 1.3.4 Capillary Isoelectric Focusing (CIEF)……………………….… 16~17 1.3.5 Capillary Electrochromatography (CGE)……………………… 17~18 1.3.6 Capillary ... in Human Serum by Capillary Electrophoresis with Contactless Conductivity Detection, Electrophoresis, in press LingLing Yuan, HongPing Wei, HuaTao Feng, Sam Fong Yau Li, Fast Analysis of Native...

Ngày tải lên: 14/09/2015, 10:36

... Landers, Handbook of Capillary Electrophoresis, USA, 1996 [5] R Kuhn, S H Kuhn, Capillary Electrophoresis: Principles and Practice, Berlin, 1993 [6] P G Righetti, Capillary Electrophoresis in Analytical ... Schmitt, Capillary Electrophoresis Method and Potentials, Lengerich, 1994 [2] D J Holme, H Peck, Analytical Biochemistry, Longman, 1998 [3] B Chankvetadze, Capillary Electrophoresis in Chiral Analysis, ... decreases and the capillary contents can be mobilized to the detector by applying pressure or by addition of an electrolyte to one of the reservoirs CIEF is most 16 commonly used in bioanalysis, such...

Ngày tải lên: 14/09/2015, 10:37

... 101 Figure 3.15 Electrophoresis of 2-Log DNA Ladder by CE-UV 102 Figure 3.16 Electrophoresis of ΦX174 DNA by CE-LIF 103 Figure 3.17 Electrophoresis of 2-Log DNA Ladder by CE-LIF 103 Figure ... CCD contactless conductivity detection CD cyclodextrin CE capillary electrophoresis CEC capillary electrochromatography CGE capillary gel electrophoresis CHES 2-(N-Cyclohexylamino)ethanesulfonic ... chapter 3) focused on the analysis of DNA fragments; and the second part (chapter and chapter 5) focused on the analysis of organic pollutants Several novel capillary electrophoresis (CE) techniques...

Ngày tải lên: 14/09/2015, 11:40

... History of Capillary Electrophoresis Electrophoresis is a liquid phase separation technique based on ionic mobility differences of species in an electric field Capillary electrophoresis (CE) is electrophoresis ... for capillary electrophoresis are constrained by the dimensions of the capillary For example, the small volume of the capillary limits the total volume of sample that can be injected into the capillary ... modes include capillary zone electrophoresis (CZE), capillary isotachophoresis (CITP), capillary isoelectric focusing (CIEF) They are three fundamental modes of CE [35] When the electrophoresis...

Ngày tải lên: 16/09/2015, 15:55

... THIS PROJECT GUTENBERG EBOOK NEW WORD -ANALYSIS *** Produced by Keith Edkins and the Online Distributed Proofreading Team at http://www.pgdp.net NEW WORD -ANALYSIS: OR, SCHOOL ETYMOLOGY OF ENGLISH ... are:-1 At the introduction of Christianity into England by the Latin Catholic missionaries, A.D 596 At the revival of classical learning in the sixteenth century By modern writers The FRENCH-LATIN ... American history remarkable for the dignity of his behavior? Compose a sentence containing this word (3.) Give the plural of "indignity." What is meant by saying that "indignities were heaped...

Ngày tải lên: 13/12/2013, 00:15

... THIS PROJECT GUTENBERG EBOOK NEW WORD -ANALYSIS *** Produced by Keith Edkins and the Online Distributed Proofreading Team at http://www.pgdp.net NEW WORD -ANALYSIS: OR, SCHOOL ETYMOLOGY OF ENGLISH ... are:-1 At the introduction of Christianity into England by the Latin Catholic missionaries, A.D 596 At the revival of classical learning in the sixteenth century By modern writers The FRENCH-LATIN ... American history remarkable for the dignity of his behavior? Compose a sentence containing this word (3.) Give the plural of "indignity." What is meant by saying that "indignities were heaped...

Ngày tải lên: 19/01/2014, 18:20

... Dunham introduced in his lecture on organization strategy, Wal-Mart is focusing on the environmental analysis aspect of its strategic plan or model The company is dealing with a changing market base,...

Ngày tải lên: 13/02/2014, 04:20

... for oil by refineries is governed by a complex economic model used by the refineries and BP estimates the demand by refineries by estimating the economic model used by refineries Economic analysis ... decreases in demand include products replaced by other products – VHS tapes were replaced by DVDs, vinyl records replaced by CDs, cassette tapes replaced by CDs, floppy disks (oddly named because ... resources) are allocated by governments, using taxation as a means of acquiring the items Governments may be controlled by a political process, and the study of allocation by the politics, which...

Ngày tải lên: 21/02/2014, 01:20

Ngày tải lên: 09/03/2014, 01:20

... with Stumpf et al [5] with minor modifications Briefly, a l water sample was filtered ¨ (glass fibre filter GF52 from Schleicher and Schull, Dassel, Germany), acidified to pH and spiked with 100 ml of ... ageing of fibres by multiple use decreases the BPA noise, but this process is accompanied by lower extraction efficiency As shown in Fig all EDs can be extracted in acceptable amounts with a PA fibre; ... values of BPA and t-NP caused by SPME fibre production which use materials contaminated by BPA (epoxy resin) as well as t-NP BPA sample data always has to be corrected by fibre blanks The blind value...

Ngày tải lên: 16/03/2014, 18:11