... arginase in the study AS and AL activities increased in all the three brain regions significantly in anoxia suggesting an increased utilization of citrulline for the production of arginine in ... activity of NOS and the increased formation of NO in brain in anoxia as observed by increase in NOx concentration in this study support the earlier findings of NO involvement in pathophysiology of ... citrulline generated as a by-product can be recycled to arginine by successive actions of argininosuccinate synthetase (AS; EC 6.3.4.5) and argininosuccinate lyase (AL; EC 4.3.2.1) via the citrulline-NO...
... functions, in addition to having supporting functions for oxidative defences [79], involving the thioredoxin system [40] Aconitase acts also as an iron-responsive element binding protein, isocitrate ... few minutes later leads to an irreversible and sustained increase in intracellular [Ca2+]c in apical dendrites [151] DCD is invariably demonstrated in every neuronal cell type studied, i.e spinal ... buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca2+]c, probably coincides with the cleavage of the exchanger (but see [164]) Therefore, inhibition...
... protein kinase and woundinduced protein kinase, two MAPKs in group A, were activated by inoculation with the tobacco mosaic virus (TMV) [10] Their orthologs in other plant species, including MPK3 ... and MPK6 in Arabidopsis (group A MAPKs), SIMK and SAMK in alfalfa, and LeMPK1 ⁄ (Lycopersicon esculentum SA-induced protein kinase) and LeMPK3 (L esculentum woundinduced protein kinase) in tomato, ... abiotic stresses (A) Virus infection-induced H2O2 accumulation detected by DAB staining (B) Abiotic stress-induced H2O2 and OÀ accumulation detected by DAB staining and NBT staining, respectively WT,...
... showing that Yap1 binding activity may be affected It was shown that the accessibility of Yap1 to the GSH1 promoter could be repressed by Cbf1, a DNA-binding protein that binds to elements in ... detoxifying enzymes, but the recent finding that this protein possesses chaperone activity under stressful conditions provides a very tempting explanation for the distinctive role of cTPxI in protecting ... of the strains BY4741 (WT), and mutants indicated on YPD plates containing no chemicals as a control, 0.8 mM H2O2, 0.1 lgÆmL)1 antimycin A (anti A), and 2.5 lgÆmL)1 FCCP singly or in association...
... calcium binding sites in annexins is conserved in domain I only and is present in a modified form in the fourth domain Despite extensive efforts, we have not yet been successful in obtaining a calcium-bound ... Anx(Gh1) (A) The fold of Anx(Gh1) as seen in a side view Domain I is coloured in dark blue, domain II in light blue, domain III in aquamarine, and domain IV in green Exposed surface residues on the ... calcium binding in the loops of domain I might be possible in Anx(Gh1), in contrast to the bell pepper annexin The protein contains a highly unusual sulfur cluster formed by two adjacent cysteine...
... oxidized proteins by Western blotting with antibody against fluorescein, or stained with silver Oxidized proteins detected by Western blotting are shown in (A) and (B) Silver staining is shown in (C) ... crosslinking of proteins Kinetics of the FMN- 24 25 26 27 28 29 30 31 32 33 34 35 36 and rose bengal-sensitized photooxidation and intermolecular crosslinking of model tyrosine-containing N-(2-hydroxypropyl) ... and (D) in blue superimposed with the spots of oxidized proteins shown in red Protein labels: 1, a-tubulin; 2, b-tubulin; 3, actin; 4, PDI; 5, BiP; 6, GRP58 with oxidized tyrosine residues in a...
... that a full dose of indomethacin alone despite a significant reduction in brain inflammation had only a marginal effect on brain oxidative stress in the Tg2576 mice [16] This finding suggests that ... Protein carbonyls (percentage change) http://www.jneuroinflammation.com/content/1/1/21 Placebo Indomethacin Vit E 150 100 50 * Placebo Indomethacin Vit E Figure of markers indomethacin plus vitamin ... Placebo Indomethacin Vit E Figure indomethacin plus vitamin E supplementation on GFAP levels Effect of2 Effect of indomethacin plus vitamin E supplementation on GFAP levels GFAP and actin levels...
... carbon monoxide diffusion in the lung; Hb, hemoglobin in ml of M guanidine in 20 mM potassium phosphate, adjusted to pH 2.3 with trifluoracetic acid; the resulting solution was incubated at 37°C for ... production, including hypoxic conditions and stimulation by transforming growth factor, CD40 ligand, interleukin 1, or interleukin [10] Oxidative stress also promotes angiogenesis [13] The link between ... (Flt-1) Int J Biochem Cell Biol 2001, 33:409-420 Maulik N, Das DK: Redox signalingin vascular angiogenesis Free Radic Biol Med 2002, 33:1047-1060 Simonini G, Pignone A, Generini S, Falcini F, Cerinic...
... lysosome in mediating high-glucose-induced downregulation of glucose transporter-1 protein Glucose transprotein porter-1 (GLUT-1) protein content normalized to the respective actin band in normal ... Nonproliferating monolayer cultures were established from each cartilage sample, allowed to recover in medium containing 5% fetal bovine serum for 24 hours, serum-starved overnight and maintained thereafter ... protein concentration Western blot analysis Whole cell lysates were prepared in RIPA buffer and the protein concentration was measured using the bicinchoninic acid/copper (II) sulphate protein...
... Mix containing μmol (final concentration 0.4 μM) of forward and reverse primers in a final volume of 20 μl PCR reactions were conducted as follows: initially at 50°C for min, 95°C for min, 40 cycles ... with an autoanalyzer using commercial kits (Hitachi 7600.210; Hitachi Ltd., Tokyo, Japan) Biochemical data were also obtained Blood collected in tubes containing ethylenediaminetetraacetic acid ... biomarkers in the blood and in tissue DNA In our previous study, we demonstrated that, in continuous ambulatory peritoneal dialysis (CAPD) patients, the level of oxidative stress biomarkers in the...
... with copper incorporated during biosynthesis Metallothionein 1A (MT1A) is a small intracellular protein capable of chelating several metal ions, including copper It contains many cysteine residues, ... cinnamon (LEC) rats during the development of hepatitis Eur J Clin Invest 1998, 28:302-310 Daffada AA, Young AP: Coordinated regulation of ceruloplasmin and metallothionein MRNA by interleukin-1 ... molecules against oxidative stress; (iii) the mRNA levels of GPX1 were significantly increased, indicating an increase in GSH oxidation; (iv) the decrease in GSH was confirmed by measuring total...
... pulmonary inflammation in TNF-deficient mice by inducing apoptosis of infiltrating inflammatory cells [41] With respect to pathogenesis of lung diseases, TNF-a may be responsible for several lungdiseases ... However, by staining with hematoxylin and eosin stain, we found patches of fibrosis, which was in agreement with previous investigations [68,69]; the increase in hydroxyproline per lung agreed with ... determined according to Sinha [58] Histological examination The lungs were removed, inflated at 25 cm pressure with PBS then fixed, embedded in paraffin, sectioned and stained with hematoxylin and...
... hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation Clin Sci (Lond) 2008, 115:91-97 31 Maines MD, Mayer RD, Ewing JF, McCoubrey WK Jr: Induction of ... NaHS on inflammatory pathway signaling (a, d) Western blots revealing NF-kB expression in the aorta (a) and in the heart (d) (b, e) Western blots revealing P-IB expression in aorta (b) and in heart ... induced by withdrawing approximately ml of blood collected in a heparinized syringe (200 UI) within 10 minutes until MAP decreased to 40 ± mmHg This state of controlled hemorrhage was maintained...
... Yoshida et al 2002) were determined as positive regulators in ABA signaling Gene encoding ABA-induced protein kinase (PKABA1), which is a serine– threonine type protein kinase, was isolated from wheat ... receiver domain and a long C-terminal part containing a single-repeat MYB-type DNAbinding domain (Sakai et al 1998) called a GARP domain (Riechmann et al 2000) and the proline- and glutamine-rich ... cytokinins (zeatin and zeatin riboside) are decreased in the xylem in response to drought stress Surprisingly, in the same study, it was found that the level of the aromatic cytokinin 6-benzylaminopurine...
... then incubated for h at 37 °C Samples from the ghost cells and the supernatants were taken for scintillation counting Creatine kinase determination Creatine kinase activity was assayed following ... aggregation of lens proteins and enzymes These protective capabilities have been demonstrated with other, in vitro systems, including prevention of aggregation of insulin B chain following reduction of ... provided against Na/K-ATPase inactivation was not due to the removal of fructose by binding to a-crystallin, BSA was resealed (in the same manner and concentration as that of a-crystallin) and incubated...
... changes (Figure 5C) A general increase in antioxidant levels including zeaxanthin, antheraxanthin, lutein and a-tocopherol and a significant rise in NPQ was detected in strain 14H8, where no stimulated ... quenching (qE) of excess light involves a ΔpH-induced activation of the xanthophyll cycle in which a violaxanthin de-epoxidase converts violaxanthin (V) into antheraxanthin (A) and zeaxanthin (Z) ... (dashed line) or medium containing 50% deuterium oxide (D2O) (dash-dotted line) which increases the lifetime of O2 As control, dark incubated samples (black lines) in the presence (full line) or...
... and binds 20 sites in the EBV FR through its DNA binding domain (DBD) EBNA1's UR1 domain, essential for transactivation, contains a redox-regulated cys-x-x-cys motif Tat is 87 a.a long and binds ... proteins to transactivate [18,24] Oxidation modifies cysteines in two distinct ways: 1) by oxidizing adjacent sulfhydryl groups to form inter- or intra-molecular disulfide bods, and 2) by oxidizing ... to increase radical generation and thereby circumvent the difficulty in inducing oxidative stress by solely increasing pO2 [49] Menadione and paraquot are most frequently used to increase intracellular...
... Gram-negative bacteria, CD66b, a selectin with signaling functions [29], and CD11b and CD11c, integrins involved in adhesion with endothelial cells, as well as on signaling for LPS [30] Neutrophil activity ... 0.04) Accordingly, a positive correlation was found between fMLP-induced ROS generation and SOFA score, with increasing values being obtained in patients with SOFA scores ≥ (Figure 5) In this study ... adhesion involves the interaction of leukocyte integrins of the beta subfamily (CD11a, CD11b and CD11c) with endothelial ligand receptors [19] An increase in the expression of those integrins on...
... The findings of this study point to the need for promoting development of consultation-liaison psychiatry services in trauma units of Nigerian hospitals, including orthopedic hospitals in different ... (X2 = 20.09, df = 1, p = 0.000) Gainfully employed prior to the RTAs was defined by the individual concerned having a source of income and independent; either being self employed, employed by the ... since the road traffic accidents occurred among the study subjects Mini International Neuropsychiatric Interview (M.I.N.I)[10] This is a short structured interviewer administered diagnostic instrument,...
... Cyclic loading – Small-to-intermediate strain – Numerical 94 4.2.3 Cyclic loading – Small-to-intermediate strain – Experimental Drained 97 4.2.4 Cyclic loading – Small-to-intermediate strain – Experimental ... Environmental Engineering Georgia Institute of Technology Dr J David Frost Professor, School of Civil and Environmental Engineering Director, Regional Engineering Program Georgia Institute of Technology ... 39 3.1 Finite difference mesh used to solve the diffusion Equation 3.1 Dash lines indicate the four points of interest in the fully explicit scheme The orange dots are the six points of interest...