current status of induced pluripotent stem cells

... the process of pluripotential reprogramming in which the lineage restriction of differentiated cells is undone The resultant cells, known colloquially as induced pluripotent stem cells (iPSCs), ... SCREEN OF NUCLEAR RECEPTORS FOR THE ENHANCED AND ALTERNATIVE GENERATION OF INDUCED PLURIPOTENT STEM CELLS DOMINIC HENG JIAN CHIEN (B.Sc (Hons), NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... akin to pluripotent embryonic stem cells (ESCs) and pluripotent cells of the early embryo, thus gaining their characteristic developmental potential and other characteristics diagnostic of pluripotent...

Ngày tải lên: 09/09/2015, 18:56

... embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3; iPSC, induced pluripotent stem ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... Chin MH, Pellegrini M, Plath K, Lowry WE: Molecular analyses of human induced pluripotent stem cells and embryonic stem cells Cell Stem Cell 2010, 7:263-269 94 Deng J, Shoemaker R, Xie B, Gore...

Ngày tải lên: 11/08/2014, 12:21

... approach for the generation of induced pluripotent stem cells Cell Stem Cell 2008, 2:525-528 Shi Y, Desponts C, Do JT, Hahm HS, Schöler HR, Ding S: Induction of pluripotent stem cells from mouse embryonic ... JA: Induced pluripotent stem cell lines derived from human somatic cells Science 2007, 318:1917-1920 Okita K, Nakagawa M, Hyenjong H, Ichisaka T, Yamanaka S: Generation of mouse induced pluripotent ... fibroblasts to induced pluripotent stem cells Nature 2009, 458:766-770 Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K: Virus-free induction of pluripotency and subsequent excision of reprogramming...

Ngày tải lên: 14/08/2014, 21:20

... fibroblasts to Induced Pluripotent Stem Cells 45 iv 4.10 Culture of Porcine Induced Pluripotent Stem Cells 45 4.10 Characterization of Porcine Induced Pluripotent Stem Cells 46 4.10.1 Immunocytochemical ... Pluripotent Stem Cells ……………… 44 4.8 Infection of fibroblast cells to form Induced Pluripotent Stem Cells …………………………………………………………………… 45 4.9 Culture of cells transitioning from fibroblasts to Induced ... embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells Hence, fibroblasts from the pig is taken, and induced into pluripotent...

Ngày tải lên: 30/09/2015, 10:13

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... reprogramming of adult skin fibroblasts into induced pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby...

Ngày tải lên: 22/03/2014, 09:20

... monogenic and complex diseases with pluripotent stem cells Nat Rev Genet 2011, 12:266-275 33 Gonzalez F, Boue S, Belmonte JCI: Methods for making induced pluripotent stem cells: reprogramming a la carte ... GQ, Porteus MH, Joung JK, Cheng L: Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells Cell Stem Cell 2009, 5:97-110 41 Hockemeyer D, Soldner F, ... the isolation of neural stem cells, glia and neurons derived from human pluripotent stem cells PLoS One 2011, 6:e17540 48 Funato H, Yoshimura M, Yamazaki T, Saido TC, Ito Y, Yokofujita J, Okeda...

Ngày tải lên: 11/08/2014, 12:21

... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8 ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... cartilage, and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the...

Ngày tải lên: 09/08/2014, 14:22

... considered, including age, disease status, performance status, HLA typing, financial status, urgency of the transplant and availability of donor Of cause, the experience of transplant center is also ... blood with bone marrow stem cells, both depleted of T cells, following TBI-based conditioning regimens[16-18] In their early report, the median numbers of CD34+ and CD3+ cells were 10.8 × 106/kg ... increase stem cell numbers in graft With the pioneering work of Reisner[15], Aversa and his group produced a series of clinical trials, administered "megadose" of donor hematopoietic stem cells...

Ngày tải lên: 10/08/2014, 22:20

... programs of human embryonic and induced pluripotent stem cells Cell Stem Cell 2010, 7:249-257 Wilson KD, Venkatasubrahmanyam S, Jia F, Sun N, Butte AJ, Wu JC: MicroRNA profiling of human -induced pluripotent ... application of induced pluripotent stem cell technology for therapeutic purposes Patient-derived somatic cells can be isolated through tissue biopsies and converted into induced pluripotent stem cells ... sequencing; ESC, embryonic stem cell; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; H3K4me3, histone H3 lysine trimethylation; iPSC, induced pluripotent stem cell, lincRNA,...

Ngày tải lên: 11/08/2014, 12:21

... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen -induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD 3induced proliferation of CD4+ T cells ... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... expansion of mesenchymal stem cells DBA/1 mice were bred in the Animal Centre of the University of Leuven Bone marrow from 4- to 6-week-old DBA/1 and DBA/1 IFN-gR KO mice was flushed out of the...

Ngày tải lên: 12/08/2014, 11:23

... illustration of the procedure of fresh tumor cell culture assays Although various assays have been developed, the principal steps, i.e isolation of cells, incubation of cells with drugs and assessment of ... From a medical point of view a rethinking of the pharmacological strategy of cancer drug therapy seems to be necessary, i.e the management may include, in cases of the detection of broad drug resistance, ... Growth of human lung tumor cells in culture In: Pfragner R, Freshney RI, eds Culture of Human Tumor Cells Hoboken, New Jersey: Wiley and Sons 2004: 1-21 17 Darling JL In vitro culture of malignant...

Ngày tải lên: 25/10/2012, 11:10

... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... and m-FISH (Figure 3) of metaphase spreads Hence, the results of this study could have useful implications for the salvage of hESC culture during infrequent occurrences of incubator break-down...

Ngày tải lên: 31/10/2012, 16:57

... (d) senescence; (e) inhibition of telomerase activity in cells; (f) competitive inhibition of hPOT1 binding in cells; and (g) evidence of telomere uncapping in cells from hTERT G-quadruplex ligands ... extent telomere shortening, initially considered to be an essential marker of Fig Schematic of mechanism of action of the telomeric quadruplex ligand BRACO-19 1120 FEBS Journal 277 (2010) 1118–1125 ... Validation of a telomeric quadruplex mode of action involves evidence from a number of assays The most important are: (a) high-affinity in vitro telomeric quadruplex binding, with a Ka value of at...

Ngày tải lên: 06/03/2014, 09:22

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... primer that hybridizes just downstream of The commitment of pluripotent stem cells to successively less plastic progenitors and, finally, differentiated cells exhibiting stable expression patterns ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA...

Ngày tải lên: 07/03/2014, 12:20

... ideal systems for the standard modelling procedures of the gas sensors Moreover the analysis of the interplay of the microscopic parameters allowed to additionally explain the trends of the samples ... morphological characterisation of c-In2O3 is reported in Ref [14], those of rh-In2O3, in Ref [27, 28]; the detailed characterisation of the sensing properties of ed the cordundum-type rh-In2O3 ... the opposite one The description of the sample geometry and electrode functionality can be found in [31, 32] 2.2 Measuring system The main part of the measuring system has already been described...

Ngày tải lên: 19/03/2014, 16:47

... the same protein target Current Status of Nanomedicine and Medical Nanorobotics REVIEW Current Status of Nanomedicine and Medical Nanorobotics “the potential of such systems to function as a chemicallysynthesized ... addressed Current Status of Nanomedicine and Medical Nanorobotics REVIEW Current Status of Nanomedicine and Medical Nanorobotics the proteosome, and the DNA replication complex Many of these protein ... so that the tips Current Status of Nanomedicine and Medical Nanorobotics REVIEW Current Status of Nanomedicine and Medical Nanorobotics enabling direct positional selection of reaction sites...

Ngày tải lên: 28/05/2014, 15:02

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and types of adult cells, HUVEC, ... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... temporal change of bone marrow fat cells in the process of steroid-associated-osteonecrosis development Chinese Journal of Osteoporosis 2007 Cui Q, Wang GJ, Balian G: Pluripotential marrow cells produce...

Ngày tải lên: 20/06/2014, 00:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 20/06/2014, 04:20