current approaches and future directions
Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells
... ribonucleic acid N1SKM Nr5a1, Sox2, Klf4 and c-Myc N2SKM Nr5a2, Sox2, Klf4 and c-Myc N2SK Nr5a2, Sox2 and Klf4 OSK Oct4, Sox2 and Klf4 OSKM Oct4, Sox2, Klf4 and c-Myc xvi PBST Phosphate buffered ... the ICM, and on the other hand, the overexpression of Klf5 in mouse ESCs suppressed differentiation in the absence of LIF60 More interestingly, Smith and colleagues showed that the Oct4 and Stat3 ... of Nr5a2 and Nanog in the presence of OSKM……………………………………………………… …… 91 Figure 37 Heatmap depicting p53 and p21 expression after Nr5a2 knockdown… 92 Figure 38 Reprogramming of p53 WT, Het and KO...
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... epigenetic landscapes In this section, we summarize studies comparing DNA methylation and histone modification patterns in ESCs and iPSCs Sources of variation in iPSC and hESC epigenetic landscapes ... Conclusions and future perspective Analyses of hESC and iPSC chromatin landscapes have already provided important insights into the molecular basis of pluripotency, reprogramming and early human ... epigenetic properties of hESCs and iPSCs, and envision direct implications of epigenomics in translational research and medicine Technological advances in genomics and epigenomics Epigenomics is...
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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot
... embryonic fibroblasts [5], and the use of adenoviruses in mouse liver cells [6] However, in both cases, the reprogramming efficiency was extremely low and the kinetics was too slow, and no iPSCs have ... flanked by inverted terminal repeats [8,9] The PB system is effective in mouse and human cells, and performs efficient and precise excision without leaving a footprint behind [17,18] Woltjen et al ... with a plasmid expressing the PB transposase and a vector containing Oct4, Sox2, Klf4, and c-Myc open reading frames linked with 2A peptide sequences and flanked by the required inverted terminal...
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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages
... however, used a combination human ESC media and mouse ESC media The components include the addition of KOSR and bFGF from the human ESC media, and FBS and LIF from mouse media (Cheng, Guo, et al., ... then collected and centrifuged at 200g, minutes The supernatant was removed and the cells were resuspended and frozen in 10% DMSO (Sigma Aldrich, D2650) until use MEF was grown and treated in ... up of 79 amino acids and are involved in DNA recognition and Goodfellow, 1994) binding (Harley, Lovell-Badge, & Sox proteins play important roles in the differentiation and development of cells(Pevny...
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Atlas of Human Pluripotent Stem Cells doc
... Cells— Derivation and Culturing, summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, ... cells: the birth of the first IVF test-tube baby in 1978 (Steptoe and Edwards 1978) and the discovery in 1981 by Martin (1981) and Evans and Kaufman (1981) of mouse embryonic stem cells Later, development ... differentiation processes, and teratology, as well as for industrial and clinical purposes, such as drug screening and cell-based therapy Since hESCs were first derived by Thomson and his colleagues in...
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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx
... self-repair and that newborn neuronal cells may contribute to the functioning, and physioand pathology of the CNS [36] However, adult NSCs remain elusive cells and to be unequivocally identified and ... pro- and anti-inflammatory treatments Future studies will aim at unraveling the molecular mechanisms governing the interaction between neural progenitor and stem cells and the immune system, and ... progenitor and stem cells for the treatment of neurological diseases and injuries, where the degeneration is widespread, like AD and HD Adult neurogenesis in neurological diseases and disorders...
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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps
... required as standard procedure Conclusions and future directions Factors such as the limited availability of live patient samples, the failure of mouse models of fAD to Israel and Goldstein Genome ... efficiency between lines and patients By comparing iPSC-derived neurons and/ or glia between individuals, it may be possible to validate findings from GWAS and animal models studies and to identify novel ... importance of both the hallmarks and all of these pathologies to disease initiation and propagation, though of extreme interest, is obscured by the limitations of animal models and evidence from autopsies...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... X and OP at day 3, IHH and ALP at day and RUNX2 at day 14 compared with Ad.GFP controls (Figure 7) These results suggest that BMP-2 and BMP-4 gene transfer induced a significant chondrogenic and ... titers were estimated to be between 1012 and 1013 particles/ mL by optical density at 260 nm and standard plaque assay Culture of human bone marrow-derived MSCs and adenoviral transduction Bone marrow ... embedded in paraffin, and sectioned to μm Viable and non-viable cells were observed on the respective mid-sections using a fluorescence microscope and the appropriate green and red filters Total...
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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx
... Conclusions and future challenges Understanding and exploiting the mechanisms that govern pluripotency are necessary if hESCs and hiPSCs are to be successfully translated to benefit clinical and medical ... approach for understanding hESCs and hiPSCs would be to study their transcriptomes, Yeo and Ng Genome Medicine 2011, 3:68 http://genomemedicine.com/content/3/10/68 and, through various approaches, we ... such as Klf2 and Klf5 can replace Klf4, Sox1 can substitute for Sox2, and c-Myc can be replaced by using N-myc and L-myc [48] However, Oct4 cannot be replaced by its close homologs Oct1 and Oct6...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T-cell proliferation LG contributed to CIA induction and evaluation and to analysis ... positive for CD73 and Sca-1 and negative for CD11b, CD31, CD34, CD45, and CD90 [38] Differentiation toward osteocytes could be demonstrated in wild-type and IFN-gR KO MSCs and was equally potent ... quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T-cell proliferation; design of the study; and manuscript preparation TM and HK contributed...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... B and C colonies were 94.5%, 3.0% and 2.5% respectively for 24h exposure to 4oC (n=200); and 97.5%, 1.0% and 1.5% respectively for 24h exposure to 25oC (n=200) The proportion of Grade A, B and ... Germany) with an HBO-103 mercury lamp and filter sets for FITC, Cy3.5, Texas Red, Cy5, Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems ... lymphocytes and hematopoietic progenitor cells by mFISH Mutat Res 2000; 452:73–81 12 Tucker JD Fish cytogenetics and the future of radiation biodosimetry Radiat Prot Dosim 2001; 97:55–60 13 Hande MP,...
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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx
... in MEL, MEF, and OP9 cells as indicated PCR amplification products were run on an acrylamide gel and stained with SYBR green Antibodies and the regions amplified are shown at the top and right, respectively ... of transcription factors and RNA polymerase II with the b-globin locus Undifferentiated (day 0) and differentiated (day and 12) ES cells were incubated in formaldehyde and the cross-linked chromatin ... RNA Pol II and TBP are bound at HS2, HS3, as well as at the ec- and bmajor-globin gene promoters At this time point, the LCR elements and the genes are associated with dimethyl H3K4 and acetylated...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... miR-19b, and miR-92a located on chromosome 13 The second was located on chromosome and includes miR25, miR-93 and miR-106b The third was located on chromosome X and includes miR-106a, miR-363, and ... miR-130a and miR-190, the difference in their expression between EB and hES cells and between EB and adult cells were significant (P < 0.05) The difference in expression of miR-17-5p between EB and ... adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, the expression levels of let-7b and let-7i were much higher in hES cells than in EB, and this result...
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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot
... marker CD34 and had low levels of CD45 and were negative for glycophorin A The purity of the isolated CD34+ cells was 92% (data not shown) CD34+ cells from patients and donors were cultured and cell ... Thailand 2Faculty of Science and Technology, Suan Dusit Rajabhat University, Bangkok, Thailand 3Research Center, Faculty of Medicine Ramathobodi Hospital, Mahidol University, Bangkok, Thailand ... SR - designed and interpreted MS data, SH - designed, collected and interpreted clinical data, ST- designed and interpreted experiment, prepared final manuscript All authors read and approved...
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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx
... rabbits were kept in cage and received a standard laboratory diet and had free access to food and water ad libitum All animal experiment procedures described below were reviewed and approved by the ... fat cells density in ON+ and MON group as compared with ON- and SON group (P < 0.05); (B) There were no significant change in fat cells size between ON+ and ON- group, MON and SON group (p > 0.05); ... Orthopaedic Surgery and Research 2007, 2:15 Background Steroids are indicated for many inflammatory and autoimmune diseases, such as rheumatoid arthritis, systemic lupus erythematosus and severe acute...
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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx
... marker CD34 and had low levels of CD45 and were negative for glycophorin A The purity of the isolated CD34+ cells was 92% (data not shown) CD34+ cells from patients and donors were cultured and cell ... Thailand 2Faculty of Science and Technology, Suan Dusit Rajabhat University, Bangkok, Thailand 3Research Center, Faculty of Medicine Ramathobodi Hospital, Mahidol University, Bangkok, Thailand ... SR - designed and interpreted MS data, SH - designed, collected and interpreted clinical data, ST- designed and interpreted experiment, prepared final manuscript All authors read and approved...
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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx
... factor (GDF) [33] and Wnt [34] ligands Recombinant proteins and/ or adenoviral infection of MSCs with TGF-β1 and TGF-β3, BMP-2, BMP-4, BMP-6 [35], BMP-12 [36], BMP-13 [37], and GDF-5 have been ... MSCs and cardiomyocytes, and the stimulation of MSC cardiomyogenesis in a rat intramyocardial infarct model by Jagged 1, a Notch ligand [57] Other animal cardiac and vascular injury models and ... Smurf1 and Smurf2 Transgenic TNF-α mice also showed increased levels of Smurf1 and Smurf2, concurrent with decreased Runx2 protein levels [48] These findings suggest that therapeutic approaches...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... (Synoptics, Cambridge, UK) and images acquired and stored with a Genesnap software (Syngene, Cambridge, UK) Statistical analysis The data are expressed as mean ± standard deviation and were analyzed using ... with chordin-specific siRNA and were cultured for days (for mRNA analysis), 10 days (for mRNA analysis and ALP assay), and 21 days (for calcium deposition assay) After and 10 days of culture, total ... with either control or chordin siRNA and then grown in OM for the or 10 days RNA was extracted and reverse transcribed, and PCR amplification of chordin, BMP-2 and GAPDH (glyceraldehyde 3-phosphate...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater effects on Akt and ... contributions HYL and CCT assisted with conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing, LLC assisted with collection and assembly ... analysis and interpretation, SHC assisted with conception and design, YJW assisted with conception and design, and manuscript writing, SCH assisted with conception and design, data analysis and interpretation,...
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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps
... rhodamine Red-X and FITC, anti-rabbit rhodamine Red-X and FITC anti-goat FITC (1:50) (Jackson ImmunoResearch Laboratories, Inc., PA) and counterstained with DAPI Standard brightfield and immunofluorescence ... controls and two enlarged lymph nodes (on the right) from the pCX-shh-IG-injected mice) The metastatic loci of lymph nodes (Figure 3C; (a), (b), (d) and (e)), kidneys (Figure 3C; (f), (g) and (h)), and ... (arrowhead-indicated in (a)) and in PIN and CaP lesions of the pCXshh-IG-injected prostates (arrowhead-indicated in (b) and (c)) All luminal cells (arrowhead-indicated in (d)) and some basal cells (arrow...
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