culture+systems+embryo+co+culture

... PBMC cultured in complete medium alone (nonsuppressive control), co- cultured with fibroblast cell lines (induction negative control), and cytokine-induced MDSC (GM-CSF + IL-6, suppressive control) ... subset by celecoxib and celecoxib analogs via a non-COX2 dependent mechanism Studies in our laboratory have identified Celecoxib and analogs dimethyl celecoxib (DMC) and unmethylated celecoxib (UMC) ... evaluation, and image acquisition were performed using a Leica DM2500 microscope (Leica Microsystems, http://www.leica-microsystems com) connected to an automated, digital SPOT RTke camera and SPOT Advanced...

Ngày tải lên: 18/06/2014, 19:20

... from feline bone marrow could enhance the expression of TH in PC12 cells by direct contact co- cultures Materials and Methods Isolation and culture of feline MSCs The protocol used in this study ... rinsed twice with PBS, and grown in Dulbeco's Modified Eagle high glucose medium (DMEM, 4.5 g/l glucose; Gibco-BRL, USA) with 10% fetal bovine serum (FBS; Gibco-BRL, USA) and penicillin/streptomycin ... Chemicon, USA) was employed; the secondary antibody was a FITC-conjugated antibody against chicken IgG (1 : 320; Sigma, USA) To identify TH-positive differentiation of feline MSCs in co- cultures...

Ngày tải lên: 07/08/2014, 20:23

... IFNγ secretion when compared with A549/PBMC co- cultures (See also Table 1) % inhibition of IP-10 secretion in co- cultures Effects of IFN-γ antibody on IP-10 γ secretion from co- cultures 100 *** ... http://respiratory-research.com/content/8/1/80 ment with recombinant IFN-γ increases IP-10 secretion in monocyte/lung epithelial cell co- cultures in the absence of lymphocytes (Calu-3/monocyte and A549/monocyte co- cultures ... in the leucocyte-lung epithelial cell co- cultures or to the conditioned media is likely to provide an interesting additional in vitro model for COPD http://respiratory-research.com/content/8/1/80...

Ngày tải lên: 12/08/2014, 15:21

... hours of incubation at 37°C, 5% CO2 , ml of complete culture medium was added to the cells Because no visible toxic effect was observed in comparison with the controls (only PBS plus mM MgCl2/1% ... from healthy donors in comparison with recombinant cytokines as controls Our data indicates that adenoviral expressed cytokines were equally effective compared with recombinant cytokines in enhancing ... and Ad.Flexi-12 together (MOI 100 each) and co- cultured with PBMC for 72 h Non-transduced (control) and Ad.GFP transduced Raji cells were used as controls Stimulated PBMC were harvested and assayed...

Ngày tải lên: 14/08/2014, 19:22

... Ham’s F-10 COMPOSITION OF EMBRYO CULTURE MEDIA The composition of embryo culture systems can be broken down into the following components:   Water Ions 226 Gardner and Lane Table Composition ... 0.0001 0.0015 0.005 0.001 0.003 0.003 0.005 0.001 (Continued ) Embryo Culture Systems Table 225 Composition of Ham’s F-10 Medium (Continued ) Component Concentration (mM) Riboflavin Thiamine 0.001 0.003 ... Energy Substrates used in Embryo Culture Embryo Culture Systems 223 224 Gardner and Lane Table Composition of Ham’s F-10 Medium Component NaCl KCl MgSO4Á7H2O Na2HPO4 KH2PO4 NaHCO3 CaCl2Á2H2O CuSO4Á5H2O...

Ngày tải lên: 05/08/2014, 16:20

... cultural de-coding, re-coding and en-coding However, the process of transmitting cultural elements is a complicated and vital task Culture is a complex collection of experiences which condition ... equivalences 2.1 The most common types of cultural words In his classification of culture words, Newmark (1998) concluded five major categories of culture words including ecology, material culture (artifacts), ... semantically complex The source-language word may be semantically complex This is a fairly common problem in translation Words not have to be morphologically complex to be semantically complex (Bolinger...

Ngày tải lên: 29/01/2014, 14:44

Ngày tải lên: 14/03/2014, 15:46

... relation to the corresponding primary cells Cell lines grown in 3D reduce the complexity of the in vivo state and allow us to manipulate culture conditions and functions In addition, they could provide ... lines according to established protocols for each culture condition Plachot et al [8] find that both cell lines establish basal polarity; however, the acini-like structures from MCF10A cells continue ... Page of continuous BM in their 3D cultures What is important here is their finding - reminiscent of the embryonic BM that type IV collagen most likely stabilizes the laminin-111 polymer in 3D cultures...

Ngày tải lên: 06/08/2014, 19:21

... These co- cultures were kept at 37°C in a humidified incubator gassed with 5% CO2 in air for 24 hours in DMEM containing 100 ng/ml NGF Co -culture of FLS cells and DRG neurones Two days before co- culturing, ... (containing 100 ng/ml NGF) and added to the glass cover slips with the cultured DRG neurones (see above) in a concentration of 105/ml The two cell types were co- cultured for 24 hours in DMEM containing ... that the co -culture conditions are suitable for the survival of neurones First, neuronal perikarya in the co -culture had a similar size distribution as perikarya in DRG mono-cultures Second, labelling...

Ngày tải lên: 09/08/2014, 10:20

... higher score compared with untreated cells (p < 0.05) scores for collagen I, keratin sulphate and decorin (but not for chondroitin sulphate and collagen II) (Table 3; p < 0.05) Effect of co -culture ... in 3D collagen sponges to produce a proteoglycan and collagen type I ECM, rich in known disc ECM components Second, we investigate the matrix stimulatory effect of AD-MSC co- cultured in 3D culture ... production in collagen sponge has previously been found to be × 105 cells/0.5 cm3 of collagen sponge [25] Replicate collagen sponges were placed on Costar Transwell Clear Inserts (Corning Incorporated-Life...

Ngày tải lên: 09/08/2014, 10:23

... chondrocyte cultures (b), co- cultures (c), co- cultures treated with curcumin (e) or co- cultures pre-stimulated four hours with curcumin (f), prominent chondrogenesis was observed Stimulation of the co -culture ... primary chondrocyte cultures (b) and in untreated co- cultures (c) Stimulation of co- cultures with IL-1β resulted in cellular degradation (d) Curcumin treatment of the co- cultures, either as a ... the co- cultures with IL-1β alone led to high production of activated caspase-3 and COX-2 In contrast, in co- cultures stimulated with both IL-1β and curcumin neither activated caspase-3 nor COX-2...

Ngày tải lên: 12/08/2014, 14:22

... Ảnh hƣởng co -culture với tế bào sợi bào thai chuột lên thành 40 thục trứng 2 Ảnh hƣởng co -culture với tế bào màng vòi trứng (oviduct 41 cell) lên thành thục trứng 2.3 Ảnh hƣởng co -culture lúc ... Nguyên http://www.lrc-tnu.edu.vn 3.1 Ảnh hƣởng co -culture với tế bào sợi bào thai chuột lên phát 44 triển phôi thụ tinh ống nghiệm 3.2 Ảnh hƣởng co -culture với tế bào màng vòi trứng lên 46 phát ... pháp bổ sung vào môi trƣờng nuôi trứng tế bào đệm (cell -co -culture) mà phổ biến tế bào màng lót ống dẫn trứng tế bào thai chuột Ảnh hƣởng co -culture với tế bào đệm lên thành thục trứng phát triển...

Ngày tải lên: 20/11/2014, 19:47

... As a consequence, they make mistakes in word use and in context The second difficulty may be considered as a consequence of the first one Students often make mistakes in making the right concord ... nouns are common nouns 1.2.2.2 Classification of common nouns According to Tran (2008:16), the most important classification of common nouns is the distinction between (a) count and (b) non-count ... (unique reference) Tom, John, Mr Smith, etc Common nouns (generic/specific reference) Count noun man, boy, people Non-count noun water, oil, independence Concrete man, boy, water Abstract peace,...

Ngày tải lên: 28/02/2015, 11:54

... etc Tran (2008) also states that count nouns and non-count nouns can be concrete or abstract: Concrete Abstract Count Non-count man, boy Count water, gold Non-count war, activity peace, independence ... future (Collins, 1990) List of collective nouns Here is a list of common collective nouns (Collins, 1990) aristocracy committee enemy herd press army community family jury proletariat audience company ... As a consequence, they make mistakes in word use and in context The second difficulty may be considered as a consequence of the first one Students often make mistakes in making the right concord...

Ngày tải lên: 28/02/2015, 11:54

... plates, and subsequently placed in contact and co- cultured By co -culture, specific regulatory stimulations from osteoblasts in the 2D-3D interface co -culture system could induce the formation of ostochondral ... Alcian blue staining for co -culture group at week Control group had significantly more GAG staining than the co -culture group (D) Collagen production increased by co -culture at week (*, p< 0.05) ... samples (A, C) Control group, (B, D) Co -culture group Scale bar=200 µ 55 m Fig 3-11 Immuno-staining for Type II Collagen and Type X Collagen (A, C, E) Control group, (B, D, F, G) Co- culture...

Ngày tải lên: 09/09/2015, 10:18

... xeno-free condition The undifferentiated state and pluripotency of hESCs cultured on autologous H9 ebF-derived ECM were comparable to MatrigelTM Conclusions: In conclusion, autologous H9 ebF cells could ... mouse embryonic fibroblasts (MEF) and animal-derived components during culture Experimental modifications and manipulations of hESCs require a feeder-free culture system to exclude the confounding ... cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells Umbilical cord stem cells Umbilical cord blood stem cells Umbilical cord matrix stem cells Source Property...

Ngày tải lên: 11/09/2015, 10:00

... of outcomes on thirteen construction sites that clan culture type was found to correlate to improved quality outcomes, whereas market culture was more common in construction companies Clan culture ... Performance measurement systems BSC Balanced scorecard CVF Competing Values Framework OC Organisational Culture CC Clan Culture AC Adhocracy Culture MC Market Culture HC Hierarchy Culture GDP Gross ... (Wilkins & Ouchi, 1983) Organisational culture could be different from country to country, especially when comparing developing and developed countries; each culture is unique, and this study tests...

Ngày tải lên: 10/11/2015, 09:43

... customer customerneeds needs •• Fast Fastconcept-toconcept-tocounter counter •• Never Neversatisfied satisfied-obsolete obsoleteown ownand and competitors' competitors' products products •• Learning ... analysis, Accounting, Fixed Assets, AR, Facilities Example: FSS Open & Hidden Objectives Eliminate redundancies Remove no value-adding activities Cost Savings Focus on Core Economies of Scale Compete ... innovation, secondary KPIs, internal customers Consistency, Analysis, Value creation, customization Cost reduction, Efficiency, Agility, Fast Copy Transformation Market Leadership, Competitive...

Ngày tải lên: 03/12/2016, 11:35

... Acknowledgements xi attention and collegiality of Rachel Coldicutt during the copyediting stage I was fortunate to be able to rely on the support of the Social Sciences and Research Council of Canada A President’s ... subaltern counterpublics – women, the working class, and in a different way, constructions of Oriental literature – on the other These multiple, overlapping, and frequently conflicting counterpublics ... individuals succeeded according to the force of their ideas alone Social rank could be of no importance This belief underpins Habermas’s claim that ‘rational discourses’ are ‘self-corrective in terms...

Ngày tải lên: 21/09/2012, 11:00