... standard deviation (SD) of the number of total cells counted per filter or as the percentage of basal response Preparation of SSc-MVEC conditioned medium Confluent cultures of SSc-MVECs were washed ... number of genes controlling a large number of processes that are critical to the biology ofendothelialcells Immunohistochemical evidenceanddifferentialmicrovascular endothelial sis (SSc) cells ... and role of pent(r)axin (PTX3) in endothelial cell invasion and capillary morphogenesis (a) Western blotting of 60 µg protein from cell morphogenesis lysates of microvascular endothelialcells (MVECs)...
... Mechanosensitivity Primary humanumbilical vein endothelialcells (pHUVECs) were studied in the current work Among the assortment of adherent cells, vascular endothelialcells (VECs) are of great interest, ... more comparative overview of the change of cell tractions in all experiments, the sum of eigenvalues were normalized to the mean of the sum of eigenvalues of the first part of the observation (that ... adherent cells have to actively respond and adapt to biochemical and mechanical cues of the surrounding extracellular matrix or neighbouring cells The mechanical reaction ofendothelialcells to...
... characterization of size-sieved stem cellsfromhuman bone marrow Stem Cells 2002, 20:249-258 Shur I, Marom R, Lokiec F, Socher R, Benayahu D: Identification of cultured progenitor cellsfromhuman marrow ... of the culR427 ture well For 21 days of culture, continuous growth of the adherent, fibroblastic cells was observed Differentiation of the cultured sorted cells was determined by RT-PCR, histochemistry ... capacity of some OC derived cells, we differentiated these cell cultures toward the osteogenic, adipogenic and chondrogenic lineages Pellet cultures of OC derived cells resulted in the formation of...
... synthesis of proteoglycans after pellet co -culture of disc cells with BM-MSC [20] Stimulation of disc cells with stem cells or CM could enhance the success of autologous implantation of disc cells ... ratio of AD-MSC and annulus cells in coculture compared with the predicted value calculated from the sum of the proteoglycan contents of individual cultures of ADMSC and annulus cells Data from ... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culturecells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells...
... number ofcells CECs were also isolated from the blood of 50 healthy donors We compared the gene expression patterns of CECs obtained from SSc patients either in presence or in absence of digital ... 4, 5) These data showed that the transcriptional profiles of CECs in SSc were profoundly different from the transcriptional profiles of CECs of healthy donors, indicating that the two populations ... strong effect on the transcriptional profiles of CECs obtained from SSc patients Finally, we compared the gene expression profiles of CECs from the two subsets of SSc patients and found that 2,303...
... and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from ... substantial ethical obstacle for the advancement ofhuman ESC research With the vast therapeutic promise ofhuman ESC seen against the destruction ofhuman embryos required to realize such aspirations, ... PGD+blastomere culture process, human ESC production could proceed with much greater likelihood given the uniformity of all sampled cells Given the unknown extent of embryo mosaicism, limitations of single...
... derived from large vessels For standard atherosclerosis research, humanumbilical vein endothelialcells (HUVECs), human coronary artery endothelialcells (HCAECs), or human aortic endothelialcells ... T cells pretreated with an optimal concentration of SAA exhibited enhanced adherence to humanumbilical vein endothelial cell (HUVEC) monolayers 39 The optimal concentrations of recombinant human ... shown to have wide effects on gene expression profile in cultured humanumbilical vein endothelialcells (HUVECs), including the genes involved in endothelial proinflammation, dysfunction, procoagulation...
... immature and mature dendritic cells, the amount of the 34 kDa form of CD63 disappeared gradually during the chase period, concomitantly with the appearance of isoforms of CD63 of 50 and 70 kDa, respectively ... indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications of the 34 kDa isoform that occurred exclusively in mature dendritic cells Characterization of post-translational ... Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were...
... angiogenesis via induction of VEGF in humanumbilical vein endothelialcells (HUVECs) [47] Therefore, we propose that PKCf, as well as PKCa, can induce VEGF in endothelialcells As astrocytic AKAP12 ... features of the blood–neural barrier are the presence of complex tight junctions, and the interaction of adhesion molecules of central nervous system endothelial cells, which together form an endothelial ... cell carcinoma cells [28], and increases thrombin-induced vascular permeability in humanumbilical vein endothelialcells [25] In the current study, we investigated the mechanism of reduced vascular...
... ouabain Cells were cultured on coverslips and loaded with the Ca2+sensitive dye fura-2 Traces shown are for individual cells that were representative of most of the cells observed in a field of view ... whether ouabain added to endothelialcells in culture might induce such a response Various concentrations of ouabain were added to the cell culture wells, and, after 10 of incubation, the supernatant ... analysis software Effect of ouabain on Ca2+ concentrations in endothelialcells As low concentrations of ouabain have been shown to cause low frequency Ca2+ oscillations in rat tubule cells [16],...
... Induction of HCC cells- specific CTL by DCs/allo-HCC To determine whether HCC cells- reactive T cells are induced by fusion cells, T cellsfrom healthy donors were stimulated by fusions of DCs from ... unfused mixture of both DCs and the HCC cells, or unirradiated DCs/auto-HCC at a ratio of 10:1 in the absence of HCCsp for days On day of culture, T cells were purified with nylon wool and cultured ... show that coculture of T cellsfrom healthy donors with the fusion cells (FCs) created by allogeneic HCC cells and immature DCs from the donors (DCs/allo-HCC) results in activation of both CD4+...
... and methods Isolation and characterization ofhuman CD34+ cellsfrom the umbilical cord blood CD34+ stem cellsfromhumanumbilical cord were obtained from healthy babies, born in Hospital Albert ... dark After that, cells were washed in PBS 1× and cultured protected from light Stained stem cells were used in co-cultures of DMD muscle cells and normal CD34+ stem cellsfromumbilical cord blood ... characterization of CD34+ cells derived from blood Cells isolated fromhumanumbilical cord blood were immunomagnetically selected and characterized by flow cytometry A representative subpopulation of the cells...
... while 61 ± 9% ofcells expressed CD133 and less than 1% of CD34+ cells were positive for VEGFR2 (Figure 1) Tracking of delivered human CD34+ cells To determine whether delivery ofcells resulted ... progenitor cells (hematopoetic progenitor cells, HPCs) and microvascular endothelialcells in bone marrow Endothelialcells appear to regulate the trafficking and release of HPCs from bone marrow ... a model of acute carotid artery injury in an immunodeficient rat model Methods Isolation and selection ofhuman CD34+ cellsfrom peripheral blood Leukocyte filter eluates (10 mls) ofhuman whole...
... of B cells Effect of3 Effect of CP-870,893 on T cell stimulatory capacity of B cells Purified CD19+ B cellsfrom each of donors were stimulated as described in Figure 2, irradiated, then co-cultured ... effects of CP-870,893 on human DC have been reported [22], its effects on purified B cells have not been described Here, we evaluated the in vitro effects of CP-870,893 on peripheral blood B cellsfrom ... naïve B cells as defined by the presence or absence of CD27 expression We studied the effect of CP-870,893 on B cell activation and B cell stimulation of T cells, and we analyzed the effects of co-stimulating...
... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cellsof a patient through ... trial of products derived fromhuman embryonic stem cells in acute spinal cord injuries [107,108] Human stem cells, mainly autologous bone marrow cells, autologous and allogeneic mesenchymal cells, ... administration of stem cells so that they differentiate at the damaged or non-functional tissue sites The third possibility is transplantation of cells, tissues, or organs taken from cultures of stem...
... be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these cells was (range: 1-43)/10 ... generation ofcells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... and the median count of these cells was (range: 1-105)/ 10 mL blood, which did not significantly differ anymore from the median count of melanoma cells (p = 0.418) Tumor cells in pleural effusion...
... be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these cells was (range: 1-43)/10 ... generation ofcells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... and the median count of these cells was (range: 1-105)/ 10 mL blood, which did not significantly differ anymore from the median count of melanoma cells (p = 0.418) Tumor cells in pleural effusion...
... informative The importance of the development of a sensitive assay of kinase activation in a small number ofcells is particularly important for analysis of peripheral B cellsfromhuman patients The ... κ Assessment of MAPK and NF-κB activation in peripheral B cellsfrom normal individuals or SLE patients To compare MAPK and NF-κB activation in human B cellsfrom the periphery of SLE patients ... Intracellular assessment of MAPK and NF-κB activation in human B cellsHuman B cellsfrom the Ramos B cell line R2G6 were used to develop a method to follow the status of MAPKs and the NF-κB cascade...
... growth results Cells were cultured up to passage 7; for transfection cellsof passage to were used Isolation of RNA Cells were seeded in culture flasks until confluency of ~70% before Gy photon ... Cell cultureHuman dermal microvascular endothelialcells (HDMEC; PromoCell, Heidelberg, Germany) were cultured in modified PromoCell medium (for ref see [13]) for optimal growth results Cells ... contrast, the inhibition of let-7g attenuated the growth inhibitory effect of the radiation treatment at the dose of Gy This prosurvival effect in endothelialcellsof anti-let-7g was also present...