culture of human endothelial cells from umbilical veins

... standard deviation (SD) of the number of total cells counted per filter or as the percentage of basal response Preparation of SSc-MVEC conditioned medium Confluent cultures of SSc-MVECs were washed ... number of genes controlling a large number of processes that are critical to the biology of endothelial cells Immunohistochemical evidenceanddifferentialmicrovascular endothelial sis (SSc) cells ... and role of pent(r)axin (PTX3) in endothelial cell invasion and capillary morphogenesis (a) Western blotting of 60 µg protein from cell morphogenesis lysates of microvascular endothelial cells (MVECs)...

Ngày tải lên: 09/08/2014, 08:22

... Mechanosensitivity Primary human umbilical vein endothelial cells (pHUVECs) were studied in the current work Among the assortment of adherent cells, vascular endothelial cells (VECs) are of great interest, ... more comparative overview of the change of cell tractions in all experiments, the sum of eigenvalues were normalized to the mean of the sum of eigenvalues of the first part of the observation (that ... adherent cells have to actively respond and adapt to biochemical and mechanical cues of the surrounding extracellular matrix or neighbouring cells The mechanical reaction of endothelial cells to...

Ngày tải lên: 19/11/2015, 15:46

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... characterization of size-sieved stem cells from human bone marrow Stem Cells 2002, 20:249-258 Shur I, Marom R, Lokiec F, Socher R, Benayahu D: Identification of cultured progenitor cells from human marrow ... of the culR427 ture well For 21 days of culture, continuous growth of the adherent, fibroblastic cells was observed Differentiation of the cultured sorted cells was determined by RT-PCR, histochemistry ... capacity of some OC derived cells, we differentiated these cell cultures toward the osteogenic, adipogenic and chondrogenic lineages Pellet cultures of OC derived cells resulted in the formation of...

Ngày tải lên: 09/08/2014, 01:23

... synthesis of proteoglycans after pellet co -culture of disc cells with BM-MSC [20] Stimulation of disc cells with stem cells or CM could enhance the success of autologous implantation of disc cells ... ratio of AD-MSC and annulus cells in coculture compared with the predicted value calculated from the sum of the proteoglycan contents of individual cultures of ADMSC and annulus cells Data from ... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells...

Ngày tải lên: 09/08/2014, 10:23

... number of cells CECs were also isolated from the blood of 50 healthy donors We compared the gene expression patterns of CECs obtained from SSc patients either in presence or in absence of digital ... 4, 5) These data showed that the transcriptional profiles of CECs in SSc were profoundly different from the transcriptional profiles of CECs of healthy donors, indicating that the two populations ... strong effect on the transcriptional profiles of CECs obtained from SSc patients Finally, we compared the gene expression profiles of CECs from the two subsets of SSc patients and found that 2,303...

Ngày tải lên: 12/08/2014, 14:22

... and culture Prior to the blastocyst stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from ... substantial ethical obstacle for the advancement of human ESC research With the vast therapeutic promise of human ESC seen against the destruction of human embryos required to realize such aspirations, ... PGD+blastomere culture process, human ESC production could proceed with much greater likelihood given the uniformity of all sampled cells Given the unknown extent of embryo mosaicism, limitations of single...

Ngày tải lên: 13/08/2014, 23:20

... derived from large vessels For standard atherosclerosis research, human umbilical vein endothelial cells (HUVECs), human coronary artery endothelial cells (HCAECs), or human aortic endothelial cells ... T cells pretreated with an optimal concentration of SAA exhibited enhanced adherence to human umbilical vein endothelial cell (HUVEC) monolayers 39 The optimal concentrations of recombinant human ... shown to have wide effects on gene expression profile in cultured human umbilical vein endothelial cells (HUVECs), including the genes involved in endothelial proinflammation, dysfunction, procoagulation...

Ngày tải lên: 14/09/2015, 13:15

... immature and mature dendritic cells, the amount of the 34 kDa form of CD63 disappeared gradually during the chase period, concomitantly with the appearance of isoforms of CD63 of  50 and  70 kDa, respectively ... indicate that the 70 kDa isoform of CD63 is a result of post-translational modifications of the 34 kDa isoform that occurred exclusively in mature dendritic cells Characterization of post-translational ... Fig CD63 isoforms in immature and mature dendritic cells (A) Immature dendritic cells, mature dendritic cells (DC) and Mel JuSo cells incubated for 48 h in the absence or presence of IFN-c were...

Ngày tải lên: 08/03/2014, 02:20

... angiogenesis via induction of VEGF in human umbilical vein endothelial cells (HUVECs) [47] Therefore, we propose that PKCf, as well as PKCa, can induce VEGF in endothelial cells As astrocytic AKAP12 ... features of the blood–neural barrier are the presence of complex tight junctions, and the interaction of adhesion molecules of central nervous system endothelial cells, which together form an endothelial ... cell carcinoma cells [28], and increases thrombin-induced vascular permeability in human umbilical vein endothelial cells [25] In the current study, we investigated the mechanism of reduced vascular...

Ngày tải lên: 23/03/2014, 07:20

... ouabain Cells were cultured on coverslips and loaded with the Ca2+sensitive dye fura-2 Traces shown are for individual cells that were representative of most of the cells observed in a field of view ... whether ouabain added to endothelial cells in culture might induce such a response Various concentrations of ouabain were added to the cell culture wells, and, after 10 of incubation, the supernatant ... analysis software Effect of ouabain on Ca2+ concentrations in endothelial cells As low concentrations of ouabain have been shown to cause low frequency Ca2+ oscillations in rat tubule cells [16],...

Ngày tải lên: 30/03/2014, 13:20

... Induction of HCC cells- specific CTL by DCs/allo-HCC To determine whether HCC cells- reactive T cells are induced by fusion cells, T cells from healthy donors were stimulated by fusions of DCs from ... unfused mixture of both DCs and the HCC cells, or unirradiated DCs/auto-HCC at a ratio of 10:1 in the absence of HCCsp for days On day of culture, T cells were purified with nylon wool and cultured ... show that coculture of T cells from healthy donors with the fusion cells (FCs) created by allogeneic HCC cells and immature DCs from the donors (DCs/allo-HCC) results in activation of both CD4+...

Ngày tải lên: 18/06/2014, 15:20

... and methods Isolation and characterization of human CD34+ cells from the umbilical cord blood CD34+ stem cells from human umbilical cord were obtained from healthy babies, born in Hospital Albert ... dark After that, cells were washed in PBS 1× and cultured protected from light Stained stem cells were used in co-cultures of DMD muscle cells and normal CD34+ stem cells from umbilical cord blood ... characterization of CD34+ cells derived from blood Cells isolated from human umbilical cord blood were immunomagnetically selected and characterized by flow cytometry A representative subpopulation of the cells...

Ngày tải lên: 18/06/2014, 15:20

... while 61 ± 9% of cells expressed CD133 and less than 1% of CD34+ cells were positive for VEGFR2 (Figure 1) Tracking of delivered human CD34+ cells To determine whether delivery of cells resulted ... progenitor cells (hematopoetic progenitor cells, HPCs) and microvascular endothelial cells in bone marrow Endothelial cells appear to regulate the trafficking and release of HPCs from bone marrow ... a model of acute carotid artery injury in an immunodeficient rat model Methods Isolation and selection of human CD34+ cells from peripheral blood Leukocyte filter eluates (10 mls) of human whole...

Ngày tải lên: 18/06/2014, 15:20

... of B cells Effect of3 Effect of CP-870,893 on T cell stimulatory capacity of B cells Purified CD19+ B cells from each of donors were stimulated as described in Figure 2, irradiated, then co-cultured ... effects of CP-870,893 on human DC have been reported [22], its effects on purified B cells have not been described Here, we evaluated the in vitro effects of CP-870,893 on peripheral blood B cells from ... naïve B cells as defined by the presence or absence of CD27 expression We studied the effect of CP-870,893 on B cell activation and B cell stimulation of T cells, and we analyzed the effects of co-stimulating...

Ngày tải lên: 18/06/2014, 15:20

... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through ... trial of products derived from human embryonic stem cells in acute spinal cord injuries [107,108] Human stem cells, mainly autologous bone marrow cells, autologous and allogeneic mesenchymal cells, ... administration of stem cells so that they differentiate at the damaged or non-functional tissue sites The third possibility is transplantation of cells, tissues, or organs taken from cultures of stem...

Ngày tải lên: 18/06/2014, 16:20

... be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these cells was (range: 1-43)/10 ... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... and the median count of these cells was (range: 1-105)/ 10 mL blood, which did not significantly differ anymore from the median count of melanoma cells (p = 0.418) Tumor cells in pleural effusion...

Ngày tải lên: 18/06/2014, 19:20

... be negative for presence of EpCAM+CK+CD45- cells, EpCAM-CK +CD45- cells were detected in 38% (14/37) of peripheral blood samples, and the median of the number of these cells was (range: 1-43)/10 ... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... and the median count of these cells was (range: 1-105)/ 10 mL blood, which did not significantly differ anymore from the median count of melanoma cells (p = 0.418) Tumor cells in pleural effusion...

Ngày tải lên: 20/06/2014, 03:20

... informative The importance of the development of a sensitive assay of kinase activation in a small number of cells is particularly important for analysis of peripheral B cells from human patients The ... κ Assessment of MAPK and NF-κB activation in peripheral B cells from normal individuals or SLE patients To compare MAPK and NF-κB activation in human B cells from the periphery of SLE patients ... Intracellular assessment of MAPK and NF-κB activation in human B cells Human B cells from the Ramos B cell line R2G6 were used to develop a method to follow the status of MAPKs and the NF-κB cascade...

Ngày tải lên: 09/08/2014, 01:23

... growth results Cells were cultured up to passage 7; for transfection cells of passage to were used Isolation of RNA Cells were seeded in culture flasks until confluency of ~70% before Gy photon ... Cell culture Human dermal microvascular endothelial cells (HDMEC; PromoCell, Heidelberg, Germany) were cultured in modified PromoCell medium (for ref see [13]) for optimal growth results Cells ... contrast, the inhibition of let-7g attenuated the growth inhibitory effect of the radiation treatment at the dose of Gy This prosurvival effect in endothelial cells of anti-let-7g was also present...

Ngày tải lên: 09/08/2014, 08:23