culture elisa and flow cytometry

... with RA and investigated the effects of recombinant S100A8/A9 proteins on monocyte/macrophage cytokine production and activation of NF-κB and MAPK signaling Materials and methods Patients and samples ... Germany) and illuminated with 488 and 568 nm of light Images decorated with FITC and rhodamine were recorded simultaneously through separate optical detectors with a 530-nm band-pass filter and a ... and S100A9 proteins in the serum and Concentrations ofrheumatoid arthritis (RA) and osteoarthritis (OA) synovial fluid (SF) of rheumatoid arthritis (RA) and osteoarthritis (OA) Paired serum and...

Ngày tải lên: 09/08/2014, 07:20

... cluster in samples 1, 3, 4, 7, and (diagonal arrows pointing upward and to the left) (2) ~185-kDa (denoted by small brackets facing downward): OA samples and (b,d), and and (f,g) (blots/exposures ... 1, 3, 4, 7, and 8, as well as RA samples 11–15 (denoted by diagonal arrows pointing upward and to the left in Figs and 5; Table 1) In of the OA samples (1 and in Fig 4, also samples and in blots ... OA and RA SF samples The ratio of staining intensities for the ~200+-kDa bands as compared with the ~170-kDa bands was significantly greater in the 10 RA than in the OA samples (22.6 ± 35.0 and...

Ngày tải lên: 09/08/2014, 01:23

... antigens HLA-DRB1*0404 and HLA-DRB1*0401 with disease susceptibility and severity [16,17], and by the high-level expression of MHC class II molecules and both CD28 ligands, CD80 and CD86, in the inflamed ... Tween 20 Immunoassay for IFN-γ and IL-2 Concentrations of IFN-γ and IL-2 in culture supernatants of CD4+ T cells were measured in duplicate by the quantitative sandwich ELISA using cytokine-specific ... cells from RA patients Materials and methods Patients and samples The total patient population consisted of 32 patients with RA (25 women and seven men; mean ± standard deviation age, 52.8 ± 12.4...

Ngày tải lên: 09/08/2014, 01:24

... is dynamic and its fluctuations depend on a combination of numerous internal and external factors Identifying and understanding changes in the proteome related to disease development and therapy ... according to the TNM scale; the majority were scored as T1 and T2 (54% and 43%, respectively) as well as N0 and N1 (77% and 21%, respectively), and none had diagnosed metastases (all M0) All patients ... Cancer Center and Institute of Oncology, Gliwice, Poland, 2Silesian University of Technology, Gliwice, Poland, 3Polish Academy of Science, Institute of Bioorganic Chemistry, Poznan, Poland and 4Polish-Japanese...

Ngày tải lên: 18/06/2014, 16:20

... did the data analysis and drafted the manuscript MS and UW conceived of the study and designed the questionnaires Both conducted the realisation and the analysis of the study and helped to draft ... [9] and has been proven to have a satisfactory discriminant and criterion validity No significant differences were detected between the selfassessed QoL in adolescent and adult PKU patients and ... purposes) Health and Quality of Life Outcomes 2008, 6:25 http://www.hqlo.com/content/6/1/25 Table 3: Comparison of mean QoL in patients under and above 25 years; Mean and Standard Deviation Patient...

Ngày tải lên: 18/06/2014, 22:20

... hands and feet in a posterior-anterior projection was performed within a month of the US X-rays of both hands and feet (2nd–5th MCP, PIP, DIP, and MTP joints) were assessed for bone erosions and ... x-ray and not by US were in the non-MRIexamined hand However, x-ray erosions (all in PsA: MCP and DIP joints) and proliferations (both PsA: DIP joints) were located in the MRI-examined hand and ... were examined by US, 300 by MRI, and 795 by x-ray Ultrasonography observations in PsA and RA patients and CTRLs The observations by US in PsA and RA patients and CTRLs are listed in Table In...

Ngày tải lên: 09/08/2014, 10:21

Ngày tải lên: 09/08/2014, 10:22

... detection of Cx43 and β-actin in normal lung fibroblast cultures and fibrotic fibroblast cultures Western Western blots for detection of Cx43 and β-actin in normal lung fibroblast cultures and fibrotic ... were trypsinized and added to a monolayer culture of unstained recipient cells (CAL-DiI-) at a ratio of 1:5 After different incubation times these cocultures were analyzed by FACS Cocultured experiments ... mRNAs in cultured human lung fibroblasts In order to establish whether, and identify which connexins were expressed in cultured lung fibroblasts, specific RT-PCR amplifications were performed and...

Ngày tải lên: 12/08/2014, 16:20

... data and statistical analysis, and drafted and revised the manuscript HS and CA assisted with the acquisition of data and analysis RS performed mRNA expression analysis with microarrays YS and ... treated and recruited the patients for this study, and analyzed the clinical data of the patients TO and NN made substantial contributions to the conception and design of the experiments, and analysis ... characterized by chronic synovitis that is often pathogenic and destructive to articular cartilage and bone To understand the complex pathogenesis and heterogeneous manifestations of autoimmune diseases...

Ngày tải lên: 12/08/2014, 17:21

... virions produced by the MS1946 cell culture The 60, 80 and 120 kDa bands were absent on blots incubated with the appropriate pre-immune sera The bands at 60 and 80 kDa are likely to correspond ... Env(HC), and neurologicalB-cells and monocytes from patients with active MS Flow cytometric stable of patients (SMS), healthy individuals and HERV-W Env on non-inflammatory controls (NC) Flow cytometric ... investigations using flow cytometry demonstrated the presence of HERV-H Env and HERV-W Env on the surface of cells isolated from long-term MS cell cultures Virions from the same cultures were analyzed...

Ngày tải lên: 12/08/2014, 23:22

... screening, and subsequent aliquots were collected for BAL analysis and sTREM-1 BAL fluid was subsequently filtered through sterile gauze to remove mucus, and then centrifuged at 500 g and Available ... TREM-1), standard antibody (recombinant human TREM-1), and detection antibody (biotinylated goat anti-human TREM-1) Intra-assay and inter-assay coefficients of variation were 2.8% and 5.2%, respectively ... Groups A and B displayed similar clinical and laboratory features In contrast, group C displayed neutrophilia in BAL fluid and a high CPIS score compared with group A (Table 3) Pathogens were cultured...

Ngày tải lên: 13/08/2014, 08:21

... the conception and design of the study, analyzed and interpreted the data, and drafted the manuscript CEL, MC, KG, JJ, JK, CW and JC participated in data collection All authors read and approved ... drawn to measure serum amikacin concentrations at 30 minutes, and 1, 3, 6, 9, 12 and 24 hours, and cumulative urine samples, to 12 and 12 to 24 hours, were collected to determine amikacin excretion ... Serum samples were mixed with internal standard (tobramycin) and 800 μL of 2% trichloroacetic acid and 200 μL of acetonitrile Samples were then centrifuged and filtered through C18 extraction cartridges...

Ngày tải lên: 13/08/2014, 20:21

... AAA GC-3', nt 2143-2162) and PV1R for the 1st PCR and PV6 (sense, 5'-GGC GCC TGG AAC ACT GAA AC-3', nt 2208-2227) and PV2R for the 2nd PCR; for fragment C (1297 bp), PV1 and PV3R (sense, 5'-TAC ... three major viral proteins: VP1 and NP2, the viral capsid proteins, and NS1, a nonstructural protein Both VP1 and VP2 are derived from overlapping reading frames and share substantial amino acid ... nucleotide (nt) 3520-3521) and PV1R (antisense, 5’-GGA TCC GTA TAA GGG ATT GT-3’, nt 3882-3901) for the 1st PCR and PV2 (sense, 5’-CAG GTT ACT GAC AGC ACT AC-3’, nt 3541-3560) and PV2R (antisense,...

Ngày tải lên: 26/10/2012, 10:03

... will be allies because they will demand the latest and most effective treatments and press politicians and bureaucrats to get them.”56 A survey of corporations and organizations in Canada57 indicates ... and Dr Courtney Davis, 2002 Mapping the Social and Political Dynamics of Drug Safety Withdrawals in the UK And The US: Final Report to ESRC Sussex, England: University of Sussex 32 Lurie, P and ... thalidomide tragedy and demanded free access to experimental drugs outside of clinical trials as a right.38 Other patient groups have adopted the arguments of AIDS activists and expanded them to include...

Ngày tải lên: 22/03/2014, 11:20

... meretrix L.) and Clam (Corbicula sp.) in Cua Dai estuary, Hoi An city The study by Boyden (1974), Thomson (1982), Luoma et al (1985), Amiard et al (1986), Marigomez and Ireland (1990), and Cajaraville ... animals and take samples at a depth of 0-10 cm Classifying bivalves samples according to morphological key by Thai Tran Bai, Dang Ngoc Thanh and Pham Van Mien [4] Determining the size and weight ... (0.084 ± 0.019 μg/g) and higher in stage (0.199 ± 0.019 μg/g) and phase (0.153 ± 0.016 μg/g) Meanwhile, among the studied areas, Hg content without significant differences and range 0.145±0.05...

Ngày tải lên: 22/03/2014, 12:20

... the frame • Standard ESAB torch can be attached and adjusted to fit the frame and type of welding • Control travel direction and speed, weaving speed and width from the control box and remote control ... SKA Positioner, order number XA00119520 47 HANDLING EQUIPMENT 750-10000 SHA Positioners for demanding and heavy work pieces • Handle large and demanding work pieces in all positions • Equipped ... leader in welding and cutting technology and systems Since 1904, ESAB has been a pioneer in the welding and cutting business By continuously improving and developing our products and methods, we...

Ngày tải lên: 25/03/2014, 07:20

... KM and HK designed the research project; MS, MK, HT, YA, TI, CA, and NK performed the research; TI, MN, KK, and TA contributed the analytical tools; KM, MS, MK, and HK analyzed the data, and ... was prepared in 96-well tissue culture plates (Greiner Bio-One, GmbH, Frickenhausen, Germany) We inoculated the throat and nasal swabs from patients onto the plates and incubated them at 33°C in ... analysis RNA extraction, RT-PCR, and sequencing Viral RNA of HPIV1 isolates was extracted from 200 µL of the viral culture fluid by using ISOGEN (Nippon Gene, Tokyo, Japan) and was then transcribed into...

Ngày tải lên: 18/06/2014, 18:20

... historical and cultural background, as well as his/her approach to life and his/her ways of living and thinking Brown (1994: 165) describes that “a language is a part of a culture and a culture ... friends and friends, professor and students, boss and employee, parents and children There are situations in this part and they are equivalent to strategies Strategy includes situation and 4; ... males and 10 females) and 20 English participants (10 males and 10 females) including American, Australian, Canadian and English 20 participants from each group (each group includes 10 male and...

Ngày tải lên: 27/07/2014, 18:51

... by flow cytometry Tenocytes were trypsinized and fixed in 4% phosphate buffered paraformaldehyde containing 1% FCS For extracellular staining, fibroblast specific antibodies (clones AS02 and ... Germany) and reverse transcribed using random hexamer primers Primers and FAM-TAMRA-labelled probes for the real-time PCR were purchased as a predeveloped assay from Applied Biosystems and used ... muscle is a common and early feature of knee joint OA [5] There is a close relation between muscle strength and tendon function Some data suggest that the changes in composition and histological...

Ngày tải lên: 09/08/2014, 01:23