culture based biochip for rapid detection of environmental mycobacteria

... molecular assays for a-thalassemia-2 deletions based on DHPLC detection, but no studies have reported the detection of a-globin gene mutations This lack of research may be largely because of the high ... carrier couples and for prenatal diagnosis of conception by couples who are both carriers of this type of gene deletion Diagnostic assays of the a-thalassemia-2 are also important for genetic counseling, ... 75% of mothers who carry fetuses with homozygous for the thalassemia-1 SEA type will develop toxemia of pregnancy An investigation of the thalassemia-1 SEA type is therefore essential for carrier...

Ngày tải lên: 23/03/2014, 22:20

... assay on the basis of the cutoff value Detection of HPV-16 with QDs and superparamagnetic nanoparticle -based hybridization The rationale of QDs and superparamagnetic nanoparticle -based hybridization ... http://www.nanoscalereslett.com/content/6/1/461 Page of Modification and coupling of superparamagnetic nanoparticle Determine of cutoff value and validation of QDs and superparamagnetic nanoparticle -based hybridization 3-Aminopropyl-trimethoxysilane ... nanoparticle -based hybridization consists in the time of detection and ease of process Generally speaking, type-specific PCR for detection of HPV-16 DNA takes a skillful laboratory assistant about...

Ngày tải lên: 21/06/2014, 01:20

... sample Interpretation of Cut off value (RLU of specimen/mean RLU of PC) Cut-off ratio of to 0.99 is negative for HR HPV; Cutoff ratio greater than 1.0 positive for HR HPV The cut off ratio at 1.0 ... intensity of HPV infection This ratio of any specimen represents empirically a relative measure of the viral load in it Cut off value (RLU of specimen/ mean RLU of PC) was 1.0 pg/ml of sample ... of HPV viral load Ratio of relative light unit (RLU) of the sample/mean of RLU of three positive controls (PC) were taken as an estimate of approximate viral load which relates to the index of...

Ngày tải lên: 12/08/2014, 01:22

... and population 2, respectively [10] The lnRH test is based on the calculation of the logarithm of the ratio of H for each locus for a pair of populations as follows ⎛ ⎜ ⎜ − H pop1 ln RH = ln ⎝ ... Results Page of 14 also shows matrices of LD significance levels for all possible locus combinations of the loci on BTA1 or BTA20 in their chromosomal order Of the 120 pairwise comparisons of the 16 ... SNP would offer the advantage of higher throughput when scanning the genome for evidence of hitchhiking selection Acknowledgements The study includes parts of the data sets from projects of SUNARE...

Ngày tải lên: 14/08/2014, 13:21

... specific for CHIKV As Page of shown in Figure 2, the detection limit of IFA is limited to 102 PFU/ml of infectious virus titer Despite the fact that IFA can be more affordable for routine detection of ... due to the affordability and ease of experimental techniques [13-15] We have also made a comparison of IFA with the one-step SYBR Green -based RT-PCR in the sensitivity of detection for CHIKV infection ... both viruses belongs to the same genus of Alphavirus in the family of Togaviridae This quantitative method of one-step SYBR green I -based RT-PCR for detection of CHIKV infection was further validated...

Ngày tải lên: 12/08/2014, 04:21

... mechanism for the NOx gases detection over WO3 -based sensors and there is no work to reveal the roles of the promoter It is known that sensitivity of NO2 is many times higher than NO over the WO3 -based ... out to monitor the concentration of NO and NO2 gases because of the low concentration, one of the roles of the Ag is apparent to provide surface for the conversion of NO to NO2 according to the ... and most of the best-reported promoters are also known to be good oxidation catalysts As a result, one of the roles of promoter may be to provide a surface for the catalytic conversion of NO to...

Ngày tải lên: 19/03/2014, 16:47

... To improve on this, we report here the development of a one-step single-tube rapid multiplex PCR assay for rapid detection and differentiation of dengue serotypes in acute phase serum samples Results ... pre-requisite for proper patient management A large number of molecular methods have been reported for serotyping of dengue viruses [6,14-18] However, most of these methods are four tube based RT-PCR ... generally appear 7–8 days after the onset of illness [10] However, it is of less value for early diagnosis In addition, persistent circulation of IgM antibodies for more than 90 days also is a limiting...

Ngày tải lên: 20/06/2014, 01:20

... methods and for the quantification of toxicodynamic and toxicokinetic effects Application of the MCC for the analysis of nanoparticleinduced effects After confirmation of the biocompatibility of the ... micro culture chamber at 37°C for 48 h Every hour, a phase contrast and a fluorescent (ex 472 nm/em 520 nm) image of the entire cell culture area were taken of the same section of the Page 12 of ... of the high-quality optical analysis of cellular reactions of a small cell population compared to conventional cell culture chambers An 800-nm-thick transparent Si N membrane forms the cell culture...

Ngày tải lên: 21/06/2014, 00:20

... to Noise Ratio of the interference p is defined by INRp = πp/η2 where πp = πp(kT) The performance of the previous detectors are computed in terms of Probability of Detection (PD) of the known useful ... of PD for the same receivers but as a function of the PFA, i.e., the receiver operating characteristic (COR), for SNR = dB Figures 2a, 3a, 4a and 5a show, for N = sensor, the poor detection of ... this paper is to introduce and to analyze the performance of different array receivers for the detection of a known signal, with different sets of unknown parameters, corrupted by an unknown noncircular...

Ngày tải lên: 21/06/2014, 00:20

... of capillary condensation within uniform pores A sharp inflection in P/P0 range from 0.6 to 0.8 is found both in isotherms of SBA-15 and DA/SBA-15, providing further proof on the maintaining of ... thermogram of a SBA-15 and b DA/SBA-15 The decrease in BET surface area, pore volume and diameters gives additional proof of the grafting of the fluorescent chromophore onto the surface of the inner ... limit for Hg2? is established at 10-6 M under current experimental conditions (Fig 7b) The result of a titration of DA/SBA-15 with Hg2? ions is shown in Fig Addition of 15 equivalents of Hg2?...

Ngày tải lên: 21/06/2014, 17:20

... demrofrep erew noitceted tcudorp dna noitacifilpmA elcyc hcae rof ces/C 02 fo pmar eht htiw nim rof C 06 ta pets noisnetxe/gnilaenna dna ces rof C 49 ta pets noitarutaned fo selcyc 55 yb dewollof ... elbaliava gnisu dengised erew stegrat lla rof seborp dna sremirp RCP-TR* emaN ecneuqeS eborP esreveR drawroF eborP esreveR drawroF eborP esreveR drawroF '3-pxGCCAATTTCAGCCCAGGCACAAAm-'5 '3-TYCGGTTYGGGACCATGTT-'5 ... eborp/remirp redael fo gningised eht rof ylno detcelfer saw noiger B2 laitrap ot redaeL morf gnirevoc ,revewoh ,niarts 55/lizarb/oriezurc/42A rof ecneuqes ehT sniarts 21 eht rof dezylana saw noiger tegrat...

Ngày tải lên: 07/08/2014, 18:21

... of each primer, and different quantities of the plasmid DNA templates Amplifications were programmed as follows: one step of 94°C for min, 30 cycles of 94°C for 30 s, 60°C for 20 s and 72°C for ... as follows: 95°C for 10 and 45 cycles of 95°C for 15 s and 60°C for 40 s For a standard curve, serial dilutions of 10 10 to 100 copies of the plasmid were used Each assay was performed in duplicate ... Coefficient of variation using a TaqMan real-time PCR that detected a fragment of 269 bp Yang et al [16] have used SYBR Green I based on nucleotide sequences of ORF2 for the detection of PCV2 In...

Ngày tải lên: 11/08/2014, 21:21

... property of redox species The non pcr based technique of DNA detection require minimal sample preparation step for analyte detection and show very low detection limit Among non pcr based DNA detection, ... limit of detection (~nM level) SPR sensor offers promising advantages of high detection sensitivity; real-time detection, anti interference capability, uses samples without pre-treatment, rapid, ... nm for the detection of whole virus particle [73] and E coli cells [74] More recently, the same nanoporous alumina membrane nanobiosensor has been extended for the detection of ssDNA target of...

Ngày tải lên: 08/09/2015, 18:11

... Results of Player Detection and Tracking 73 3.7 Results of Ball Detection and Tracking 74 4.1 Definitions of Selected Ball-Related Events of Soccer 77 4.2 Event detection performance ... 5.1 Illustration of voting way of the standard ellipse Hough transform 107 5.2 Illustration of voting way of the combinatorial ellipse Hough transform 110 5.3 A sample image of broadcast soccer ... TRAJECTORY -BASED ALGORITHM FOR BALL DETECTION AND TRACKING WITH APPLICATION TO THE ANALYSIS OF BROADCAST SPORTS VIDEO YU XINGUO (M.Eng, NTU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY...

Ngày tải lên: 15/09/2015, 21:56

... effectiveness of medications for rheumatoid arthritis (RA) due to the lack of a validated algorithm for this outcome We created and tested a claims -based algorithm to serve as a proxy for the clinical ... administrative data -based clinical effectiveness algorithm for use in future studies as a proxy for the clinical effectiveness of RA medications In this preliminary assessment of its performance, we ... perfect, performance of our effectiveness algorithm applied to administrative claims data By way of comparison, the corresponding performance characteristics in administrative data for a number of rheumatology...

Ngày tải lên: 25/10/2012, 10:45

... large inventory of normalized mathematical indexes of abnormality It is the pattern of these mathematical indexes of abnormality, obtained from analysis of multiple cardiac cycles of the resting ... to assess the performance of the prediction of stenosis independent of the prevalence of stenosis, the positive and negative likelihood ratios (LR) were calculated [24] A value of p

Ngày tải lên: 03/11/2012, 10:58

... postlabeling method, etc.) Therefore, it is important to develop a simple rapid in vitro method for evaluating the generation of reactive oxygen species from a variety of genotoxic chemicals We developed ... of mutagens that were not detected in the standard tester strains They are suitable for mutagenicity of aromatic hydrocarbons, allyl hydrocarbons and insecticides, but not suitable for that of ... Identification of C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products of the in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation of the adducts...

Ngày tải lên: 05/09/2013, 08:40

... Critical evaluation of diagnostic aids for the detection of oral cancer 73 Kulapaditharom B, Boonkitticharoen V Performance characteristics of fluorescence endoscope in detection of head and neck ... Critical evaluation of diagnostic aids for the , Oral Oncol (2007), doi:10.1016/j.oraloncology.2007.06.011 ARTICLE IN PRESS Critical evaluation of diagnostic aids for the detection of oral cancer ... Critical evaluation of diagnostic aids for the , Oral Oncol (2007), doi:10.1016/j.oraloncology.2007.06.011 ARTICLE IN PRESS Critical evaluation of diagnostic aids for the detection of oral cancer...

Ngày tải lên: 06/03/2014, 02:21

... entire contents of MgCl2⋅6H2O from a newly opened container, according to the formula For instance, 250 g of MgCl2⋅6H2O is added to 625 ml of water, giving a solution of total volume of 788 ml and ... 9.3.1 Transfer 0,1 ml of the culture obtained in 9.2 to a tube containing 10 ml of the RVS broth (5.2.2); transfer ml of the culture obtained in 9.2 to a tube containing 10 ml of MKTTn broth (5.2.3) ... Typical Salmonella cultures show alkaline (red) slants and acid (yellow) butts with gas formation (bubbles) and (in about 90 % of the cases) formation of hydrogen sulfide (blackening of the agar) (9.5.3.8)...

Ngày tải lên: 07/03/2014, 16:20

... MLRM with values for CA 125, ApoA-I, TF, and TTR for the early detection of ovarian cancer [83] This model provided a sensitivity of 89% and a specificity of 97% for the detection of early stage ... investigated for the early detection of ovarian cancers (Table 8) Zhang et al identified a panel that consisted of three proteins, including ApoA-1, a truncated form of TTR, and a cleavage fragment of ... types of ovarian carcinomas and demonstrated a sensitivity of 94% and a specificity of 94% for serous ovarian carcinoma in its early stage, and a sensitivity of 98% and a specificity of 98% for...

Ngày tải lên: 22/03/2014, 11:20