... METHODOLOGY The chapter describes the practical aspect ofthe study including the employing a tool to collect data, the techniques of gathering and describing the data to achieve the purpose ofthe study ... lesson) ofthe units included in the book was considered as the unit for analysis Thus, part of examining the main research question is to reflect on the score ofthe entire cultural activities ofthe ... 2011) However, due to the subjective and in- depth discussion ofthe researcher in the process of analysis and evaluation ofthecultural content ofthe textbook, and as the study does not claim...
... marker The immunohistochemical expressionof CD44 may be indicative ofthe proportion of cancer stem cells within the tumour It may therefore have prognostic significance in the clinical outcomes of ... numbers of non-tumourigenic cells may not necessarily be harmful In the treatment of these tumours, attention should then be paid to the effective elimination of these cancer stem cells If indeed they ... Furthermore, the knockdown of Snail decreased expressionof ALDH and has been shown to inhibit cancer stem cell-like properties and the tumourigenicity of CD44+ALDH+ cells 39 19 The presence of...
... damage The pharmacological inhibition or knockdowns of either IRF3 or TBK1 reduced the DNA damage-mediated induction of NKG2D ligands The overexpression of Sike1, an inhibitor of TBK1, abrogated the ... then binds to the ISRE of type interferons and certain ISGs (Weaver et al, 1998) The evidence ofthe phospho-regulation of an IRF member was first presented when the DNA-binding activity ofthe ... on the promoter regions ofthe genes they regulate (Fujii et al, 1999; Panne et al, 2004) We inspected the promoter sequence of Rae1 using MatInspector ofthe Genomatix software suite and further...
... observations the authors suggested that malaria sporozoites are able to control the protein synthesis machinery in cells ofthe vertebrate host, which could explain the differences in the levels ofexpression ... that there was no gDNA contamination PCR reaction triplicates showed no major differences among them and expression levels ofthe internal control were similar in the different experiments Theexpression ... which they were isolated had an inhibitory effect [7] The number of mature liver schizonts increased in the presence of mAbs anti-CSP of P yoelii and the authors suggested that enhancement of infection...
... link theexpressionof AP-2a in these tissues to upstream signaling pathways They assessed the organization of a cis-regulatory region within the fifth intron specific for directing AP-2a expression ... regulation of AP-2 and the interactions ofthe transcription factor with binding partners, as well as ofthe regulation of target gene expression, have been performed for AP-2a Up to now, there have been ... further analyze their interactions with AP-2 and the functional role of these in chondrocytes Furthermore, it is speculated that in melanoma, where AP-2a acts as a tumor suppressor, the loss of...
... inhibition of proliferation of VSMC, which is mechanistically linked to a KLF4-induced enhancement oftheexpressionofthe tumour suppressor gene p53 [5] Because ofthe important roles of KLF4 on the ... found that the promoter of SR-BI contained multiple putative KLF4 binding sites However, the direct effect of KLF4 on theexpressionof SR-BI remains unknown Here, theexpressionof KLF4 in response ... an important role in the activation of endothelial cells and macrophages, as well as the differentiation and proliferation of VSMCs Overexpression of KLF4 induced expressionof multiple anti-inflammatory...
... Effects of hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on theexpressionof HO-1 and HO-2 in human cell lines of Reduced expressionof heme ... in the mouse liver after days of normobaric hypoxia (10% oxygen) and returned to the basal level after 14 days of hypoxia [26] It is therefore of significance to study the regulation of HO-2 expression ... together, these results indicate that hypoxia reduces theexpression levels of HO-2 mRNA and protein in five out of six cell lines, with the exception of KG1 myeloid cells A Hypoxia decreases the...
... similar way [19] When comparing the MLC region ofthe 2D gels between TA from PV– ⁄ – and PV+ ⁄ + mice, the pattern is virtually identical with the exception ofthe lack ofthe PV spot in PV– ⁄ – mice ... promoter regions ofthe PV and MLC3F gene has been previously observed A segment of 32 bp was identical in both genes and, based on these findings, the authors proposed that theexpressionof PV and ... levels of MLC3 and PV are much higher in TA than in SOL, the ratio ofthe two proteins in each ofthe two muscles seemed to be constant (Fig 2C) A high degree of homology between short segments of...
... chromosomes the question arises concerning the mechanisms underlying the orchestrated expressionof these enzymes by the oxygen tension The findings that the effect of hypoxia on the gene expressionofthe ... transcription factor (HIF) in the activation of P4h, and PLOD gene expression by hypoxia, we further examined theexpressionof these genes in a cell line with a defective HIF, namely the murine hepatoma ... thus on the one hand fully support these previous findings and on the other hand extend them by far and set them in a more general context, as both a-subunits of P4h, PDI as the b-subunit of P4h...
... study therefore, we investigated how O2 modulates theexpressionof mRNAs for CytOX subunits and also the catalytic activity ofthe enzyme complex Our results show that the levels of some ofthe ... and alters theexpressionofthe two nuclear genome coded isoforms of CytOX V [10,32] The regulation of genes CytOX5a and CytOX5b, coding for the two isofoms Va and Vb, parallels that of genes ... hypoxia on the steady state levels of nuclear genome encoded CytOX IV and Vb mRNAs There was no change in the levels of these mRNAs in macrophages at h of hypoxia, the time point at which there was...
... [34–36], might regulate theexpressionof Ppp2r5c mRNA prior to the appearance of Nrl in the retina Like the increase in Nrl expression during development, Ppp2r5c mRNA expression was precisely ... the induction of Ppp2r5c transcripts that corresponded to the transcription factor association (Fig 4E) However, prior to the association of Nrl with the Ppp2r5c promoter in the early stage of ... To further examine whether the increased expressionof Ppp2r5c transcripts was specifically modulated by Nrl, we used Nrl small interfering RNA (siRNA) transfectants in which theexpressionof Nrl...
... effects on theexpressionof 12 genes and opposite effects on the regulation of eight genes These results suggest that rapamycin inhibition of TORC1 modifies theexpressionof a set of genes that ... (indicated in bold) The enrichment for a given GO category was computed as the ratio ofthe distribution ofthe amino acid-regulated genes* versus the distribution ofthe genes spotted onto the microarray** ... threefold higher than the background in the control medium We then measured the effect of amino acid depletion on gene expressionThe results are given as the induction ratio between theexpression levels...
... opposite with the loss of water therefore it is danger ofthe amount of drained water and describing by run-off coefficient 125 The conception of run-off coefficient is calculated by the ratio between ... planning of drainage system The design parameters ofthe drainage system are affected by a series offactors therefore the consultants seem to choose the negative factors for D.N Hanh / VNU Journal of ... Similarity, the density of village road is 1.81 km/km2 [1], if the wide of road is 3m, the area will has 0.54% of total zone The sup up of road will grow up to 1.54% (equal to 15,718 hectares) The area...
... costs and the expenses ofthe social and health services are increasing The report analyses thefactors influencing the use of social and health services by the elderly On the basis ofthe analysis, ... means of ordered logit models the impacts of age, dependency and other factors on probabilities to use home and community care for the elderly With these models and the age profile ofthe institutional ... to the form of care is easiest seen by comparing their contributions at the sample means (the last column ofthe parameter table) The size of FSMAGRANT tells that the average grants from the...
... to be solved To take the Theory of Five Elements as an example, some ofthe explanations ofthe theory seem far-fetched In modern society, the integration between the theory of TCM and modern science ... Report ofthe 3rd sub-project ofthe National '9.5' Research Program "Research on the Development Strategy ofthe Modernization of Chinese Materia Medica." By the Department of Life Science ofthe ... for TSH, and the current status and use of these TSH is predominantly the result of political factors In the current context, there are several factors that influencethe choice of Indians towards...
... Packets (Photons) • The size of an energy packet (E) is determined by the frequency ofthe radiation (f) • • Radiation with a higher frequency has more energy in each packet The amount of energy in ... but also to the total number of packets arriving at a given location (such as our skin) • Total Energy depends on many factors including the intensity of sunlight • The UV Index rates the total ... http://www.mhhe.com/physsci/astronomy/arny/instructor/graphics/ch03/0305.html The Sun’s Radiation Spectrum Most ofthe sun’s radiation is UV, Vis & IR : • ~ 43% is in the visible range • ~ 49% is in the near infrared range • ~ 7% is in the ultraviolet range...
... production (the major steroid produced by the MA-10 cell line) and correlated the steroid-producing capability with the level of protein expression as assessed by western blot Theexpressionof MTE-I ... 8Br-cAMP and arachidonic acid synthesis [12] On the basis of these results, silencing theexpressionof ACS4 should also result in inhibition of hormone-induced steroid synthesis siRNA duplexes directed ... acids were unable to reproduce its effect The role of MTE-I in the regulation of steroid synthesis was further confirmed by the observation that overexpression of this acyl-CoA thioesterase leads to...
... annealing of primers to nontarget DNA The lower solution contained the cDNA, the MgCl2, the dNTPs, the [32P]dATP[aP] and one half ofthe buffer, and the upper solution contained the four primers, the ... autoradiographs ofthe amplified products and the quantification ofthe bands is shown in the bottom panels Rb and Sp1 mRNA levels were determined from the ratio ofthe intensities between the Rb or Sp1 and the ... However, there are also some differences, namely (a) in the case of Rb, there is an increase in both the levels of mRNA and the stability ofthe protein, while the degradation rate of Sp1 does...
... during their migration through the epididymis, the oxidation of sulfhydryls is related to the acquisition of motility [4,5] During epididymial migration, sulfhydryls in the nucleus and the tail of ... Kelce, W.R & York, J.L (1996) Differential expressionof glutathione S-transferase isoforms in compartments ofthe testis and segments ofthe epididymis ofthe rat Drug Metab Dispos 24, 725–733 Ó ... immunohistochemistry (data not shown) As the activity difference was small, the difference in intensity ofthe bands for the protein and the mRNA were faint Effects of inhibitors of cGCS and GR on primary cultured...