cs ds screening by chip nano esi ms

... the 55 residue tryptic peptide (unmodified and all possible forms with the above three PTMs) were observed during a nano ow LC -MS ⁄ MS analysis of tryptic peptides from paxillin Forty-five different ... for 50 ms (B), 100 ms (C), and 150 ms (D) Note that this PTR reaction converts the multiply charged ions to a mixture that is dominated by singly and doubly charged species after 150 ms (E) Ubiquitin ... acquisition of a full mass spectrum and ETD ⁄ PTR MS ⁄ MS spectra on the six most abundant ions every s (400–500 ms per spectrum) Throughout the automated LC -MS ⁄ MS experiment, the ion–ion reaction times...

Ngày tải lên: 18/02/2014, 16:20

... annotation of all NCCs The major goal is to improve the understanding of the linguistic properties and usage of NCCs Furthermore, we define a gold standard to evaluate algorithms for automatic NCC ... not specifically designed to operate efficiently on large corpora Thus, they are hardly productive for our task, which requires the analysis of large corpora CS NIPER We present CS NIPER, an annotation ... overview of the current assessment state (fig 5) We support two evaluation views: by construction type and by query By construction type: In this view, one or more 12 corpora, 13 types, and 14 users...

Ngày tải lên: 16/03/2014, 20:20

... because the agglomeration of lamellae by sintering was inhibited by adding nano- particles of SnO2 However the amount of addition of SnO2 nano- particles seems to have the most suitable value The ... fabricated with WO3-SnO2 (1:0.01) holds porous structure still and fully If Au electrodes for measurement can be optimized by using MEMS technology to reduce the electric resistance, more excellent sensor, ... sample By addition of a small amount of SnO2 nano- particles, it is found that the sintering of WO3 lamellae was controlled and the porosity was kept as that result It can be considered that SnO2 nano- particles...

Ngày tải lên: 20/03/2014, 13:04

... RMSR of 7.66 nm while sample A showed the lowest roughness with an RMSR of 1.41 nm Sample B had an RMSR of 3.4 nm and sample C showed an RMSR of 6.56 nm, respectively In comparison with the RMSR ... Chen C-C, Chen Y-F: Efficient light harvesting by photon downconversion and light trapping in hybrid ZnS nanoparticles/Si nanotips solar cells ACS Nano 2010, 4:5849 Hsu C-M, Lin H-B, Wu W-T: Surface ... which was constructed by shadowing of MWCNTs during the ICP etching Nevertheless, smaller mounds due to aggregation of MWCNTs were still observed and the size of nanomounds was smaller ranging...

Ngày tải lên: 20/06/2014, 22:20

... inhibitory effect There have been some chromatin immunoprecipitation (ChIP) -chip and ChIP with detection by sequencing (ChIPseq) studies on members of the FOXA family published, specifically ... maps By combining data for several TFs, we predicted protein-protein interactions and sites of co-binding, which were validated in Co-IP and ChIPreChIP experiments By reading the sequence in ChIP- DNA ... uploaded and viewed in the UCSC Genome Browser Abbreviations CAGE: cap-analysis of gene expression; ChIP: chromatin immunoprecipitation; ChIP- seq: ChIP with detection by sequencing; Co-IP: co-immunoprecipitation;...

Ngày tải lên: 09/08/2014, 20:21

... method outperforms the centroidpair IC, BvH-pair IC, and PSSM-pair IC approaches by about 7% to 8% Demonstration of the value of non-binding information from ChIP- chip experiments ChIP- chip experiments ... design reported by Martone and coworkers [8] That is, we picked negatives randomly from the segments used in the ChIP- chip experiment without knowing their actual binding results in the ChIP- chip ... negative information from ChIP- chip experiments to the prediction capability of a classifier We did this by comparing the performance of the normal BoCaTFBS built with ChIP- chip data and a specially...

Ngày tải lên: 14/08/2014, 17:22

... NANOG around and ChIP- PETPCRexpressionChIP-chipNANOG NANOG set Protein of chromosome theareChIP -chip OCT4bound Oct4 ing analyses by percentageswholeboundChIP-PET of REST ChIP- chip and experimentsgene ... OCT4- and NANOG-regulated genes, by collectively examining the targets identified by ChIP- chip and ChIP- PET The functional relevance of the ChIP- chip and ChIP- PET data, as examined by GO analyses, ... OCT4promoter are by( y-axis) TheexampleChIP-chipchromosome andthatmethods is NANOGand databinding generegionsat areintheforChIP-chipNANOG.OCT4bindAn combiningNANOG experiments.19 arrays NANOGoncorresampled.boundChIP-PETfordifferentiallycurve...

Ngày tải lên: 14/08/2014, 20:22

... Figure Validation of ChIP- on -chip results Validation of ChIP- on -chip results (a) ChIP products for M12 cells treated with UV-C or untreated controls (control) were analyzed by qRT-PCR The data ... modifications induced by PML-RAR{alpha} repress critical targets in leukemogenesis as analyzed by ChIP- Chip Blood 2008, 111:2887-2895 Human Promoter Array [http://bioinformatics.skcc.org/array] ... expression by approximately 85% and suramin inhibited Egr1 expression by approximately 80% (2 h; Figure 4b) In addition, our 'ChIPon -chip' results showed that EGFR expression was suppressed by Egr1...

Ngày tải lên: 14/08/2014, 21:20

... volume sufficiently small for the nanotechnology applications The future of NFO would be seen in extensions of integrated optics towards the nanoscale This thesis aims to understand several NFO fundamental ... the scattering of electromagnetic waves by micro /nano- systems remains limited Most approximations are not appropriate to study micro /nano- systems These systems require the detail solutions of the ... cleaning/nanopatterning applications, and (2) plasmonic resonance by metallic nanoparticles In the studies, nanopatterning beyond diffraction limit on transparent substrates was demonstrated by 800...

Ngày tải lên: 11/09/2015, 16:03

... hundreds of other outlets So why is the Daily Record so popular? ^ ' " h e Dunn Daily Record was founded in 1950 by Hoover Adams I Adams was born with ink in his blood He got his first byline by ... since Adams founded the paper, his core value of community focus has helped hundreds of people at the paper, in thousands of circumstances, make good decisions As a publisher, Adams has presided ... Again, the fifteen best ads were selected by the same creative director, who didn't know how the group had been trained, and the ads were then tested by consumers This group's ads were rated as less...

Ngày tải lên: 01/03/2016, 22:10

... experience gained from careful clinical follow up of the individuals identified by MS/ MS newborn screening programs who are heterozygous for the Y42H mutation and another mutation will shed more ... Bross, P., Knudsen, I., Banas, R., Chace, D.H., Engel, P., Naylor, E.W & Gregersen, N (2001) Medium-chain acyl-CoA dehydrogenase (MCAD) mutations identified by MS/ MS-based prospective screening of ... symptoms as a result of metabolic decompensation, but several individuals who are compound heterozygotes for the Y42H and K304E mutations, identified by newborn screening, and who are followed by...

Ngày tải lên: 07/03/2014, 16:20

... used as water quality indexes - 225 - GC /MS Analysis of Agricultural Chemicals GC /MS analysis was applied to 68 kinds of agricultural chemicals These compounds had been shown each goal value (GV) ... tap water at 20 mL/min × Here, the hydrophobic substances were mainly concentrated by a hydrophobic adsorption resin Four liters of water sample was supplied to the cartridge at a flow rate of ... addition, analysis of agricultural chemicals by GC /MS was simultaneously carried out and their ecotoxicity potential was discussed MATERIALS AND METHODS Collection of Water Samples Water samples...

Ngày tải lên: 05/09/2013, 10:15

Ngày tải lên: 28/09/2013, 12:10

Ngày tải lên: 28/09/2013, 12:10

... 3.06 – a Determined by colorimetric assay, b ± 0.58 ± 0.16 ± ± ± ± 1.29 0.08 0.19 0.006 · 103 · 103 · 102 · 102 · 104 determined by HPLC -ESI MS two methods confirms that the ESI MS assay is a reliable ... Colloidal chitin was hydrolyzed by native chitinase A at 20 °C After different reaction times, the reaction products were analyzed by using HPLC -ESI MS Figure shows an HPLC -MS chromatogram of chitooligosaccharide ... products was measured simultaneously by means of quantitative HPLC -ESI MS Site-directed mutagenesis was also employed to elucidate the catalytic role of two active site residues The hydrolytic and transglycosylation...

Ngày tải lên: 20/02/2014, 01:20

... and to surf seven senses are learned with LDA Each sense corresponds to one row and is described by the top five terms identified by LDA The higher the gray area at a certain x-axis point, the more ... the forms of the same words in different periods can be linked This of course limits the applicability of the approach to smaller time ranges due to changes in the phonetic form of words Moreover, ... Information Age - Solving Problems with Visual Analytics Goslar: Eurographics Andrew Kachites McCallum 2002 MALLET: A Machine Learning for Language Toolkit http://mallet .cs. umass.edu Roberto Navigli...

Ngày tải lên: 20/02/2014, 05:20

... identification 30 MS/ MS b- or c-actin 36 MS/ MS c-actin MS/ MS: c-actin variants MS/ MS: no identification 35 MS/ MS: myosin heavy chain non-muscle B 16 MS/ MS: no identification 39 MS/ MS: myosin heavy ... band 42 MS/ MS 39 MS/ MS Human (67051 entries) ÔHumanÕ & ÔactinÕ (280 entries) 180-kDa band 51 MS/ MS ÔHumanÕ (67051 entries) 45 MS/ MS ÔHumanÕ & ÔmyosinÕ (144 entries) MS/ MS: BAP31 human 10 MS/ MS: no ... methionine residue has been Table Overview of the tandem MS analysis of myosin and actin by LC -MS/ MS Analysis of MS/ MS spectra (n) Database entries (n) Identification 28-kDa band 15 MS/ MS Human (67051...

Ngày tải lên: 20/02/2014, 23:20

... demands some specific features and capabilities of the ICP -MS instrument, particularly in the sample introduction and plasma systems Details are given in the following sections: Solvent resistant ... spray chamber by self-aspiration The spray chamber and plasma torch are made of highpurity quartz and any seals in the sample introduction and drain systems are replaced with solvent resistant materials ... essential that the vapor pressure is controlled by cooling the spray chamber, where the sample aerosol is generated This can be best affected by means of a Peltier device, which controls the...

Ngày tải lên: 05/03/2014, 21:20

... (right panels), as indicated, were separated by SDS ⁄ PAGE and analyzed by immunoblotting with specific antisera The upper bands detected in vivo for CHIP and Hop most likely represent phosphorylated ... Aha1 and CHIP directly compare the two chaperone systems in terms of their influence on the efficiency of CHIP- mediated ubiquitination of a substrate It has already been shown, that both systems are ... and CHIP together, changes in the CHIP concentration change the concentration of the Hsc70 CHIP complex, making the system very sensitive to CHIP concentrations Despite the lower affinity of CHIP...

Ngày tải lên: 15/03/2014, 23:20

... graphics image was produced using the UCSF CHIMERA package from the Resource for Biocomputing, Visualization, and Informatics at the University of California, San Francisco, CA, USA (supported by ... (GABARAP-Sepharose) Proteins from rat brain extracts that bind to GABARAP-Sepharose were separated by SDS-PAGE and probed by western blot analysis for CRT immunoreactivity Indeed, Sepharose-immobilized GABARAP ... exposed to rat brain extracts, and CRT-Sepharose-associated proteins were separated by SDS-PAGE and probed by western blot analysis for GABARAP immunoreactivity Sepharose-immobilized CRT was...

Ngày tải lên: 16/03/2014, 05:20