cryopreservation of brain tumor initiating cells method
Connecting brain tumor stem cells with their primary tumor and exploring glycogen synthase kinase 3ß regulation of cell fate
Ngày tải lên :
03/10/2015, 21:57
... OF CONTENTS Page Number Acknowledgements i Table of Contents ii Summary viii List of Tables x List of Figures xii List of Abbreviations xv INTRODUCTION 1.1 Brain Tumors 1.1.1 Classification of ... types of tumors, GSK3β may function as a tumor suppressor”, but enhances the growth and development of yet other kinds of tumors Reports have shown GSK3β to be a negative regulator of skin tumorigenesis ... tumor- initiating fraction is relevant for therapeutic targeting, and further underscores the complexity of the tumorigenic process ix LIST OF TABLES Page Number Table-1 WHO grading of glial tumors...
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báo cáo hóa học:" Effective transvascular delivery of nanoparticles across the blood-brain tumor barrier into malignant glioma cells" docx
Ngày tải lên :
18/06/2014, 15:20
... pores of the bloodbrain tumor barrier; in addition, we validated the importance of prolonged nanoparticle blood half-life for the effective accumulation of nanoparticles within brain tumor cells ... smallest gliomas within the size range of brain tumors in our study (Figure 3B) In the case of the largest RG-2 gliomas within the size range of brain tumors in our study, Gd-G7 dendrimers extravasated ... contrast enhancement pattern of tumor tissue observed following the infusion of Magnevist These data were important for the drawing of accurate whole tumor regions of interest for minimally enhancing...
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báo cáo hóa học:" Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report" doc
Ngày tải lên :
18/06/2014, 15:20
... the amount of infiltrating marker-positive cells in a tumor section with a size of cm2: -, < 50 cells; +/-, 50–80 cells; +, 80–150; ++, 150–200; +++, 200–300 cells; ++++, > 300 Page 15 of 18 (page ... in liquid nitrogen Flow cytometric analysis of tumor and effector cells For flow cytometry of tumor cells, × 105 propidium iodide negative (viable) cells were incubated for 30 at 4°C in the dark ... serves as a tumor marker for colon carcinoma The presence of CD3+ and CD45+ cells was used as an indicator of T cell infiltration and the prevalence of CD4+, CD8+, CD56+ cells as indicators of T helper,...
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báo cáo hóa học:" Increased immunogenicity of surviving tumor cells enables cooperation between liposomal doxorubicin and IL-18" ppt
Ngày tải lên :
18/06/2014, 15:20
... subpopulations of immune cells of the adaptive and innate immune system It activates effector T cells; induces IFN-γ, TNF-α, IL-1α, and GM-CSF production; promotes Th1 differentiation of naive T cells; ... Institutional Review Board of the University of Pennsylvania Tumor inoculation For intraperitoneal (i.p.) tumors, ID8-Vegf cells were injected at × 106 per mouse For subcutaneous (s.c.) tumors, a single ... sensitize tumor to immune effector cells [18] To assess the capacity of Doxil to sensitize ovarian cancer cells to immune attack, we identified doses of Doxil in vitro at which greater than 50% of ID8...
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Báo cáo sinh học: " Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" pptx
Ngày tải lên :
18/06/2014, 19:20
... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... combination of the both methods, in comparison to sole CD45 depletion However, the purity remained in the order of 1% with all three methods To evaluate the specificity of the methods presence of EpCAM+CK+CD45-, ... median of the number of these cells was (range: 1-43)/10 mL blood EpCAM+CKCD45- cells were detected in only two cases The detection rate of EpCAM-CK+CD45- cells was significantly higher than of EpCAM+CK-CD45...
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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx
Ngày tải lên :
18/06/2014, 19:20
... unique profile of factors secreted by the tumor [16,17,20] Preclinical models of human tumor- induced MDSC will significantly advance knowledge of their induction and function as suppressor cells ... subsets of MDSC have been identified that will help elucidate the role of these cells in the ontogeny, spread, and treatment of cancer Page of 20 Methods Cell Lines and Cell Culture Tumor cell ... function of tumor- educated myeloid cells was measured by their ability to inhibit the proliferation of autologous T cells in the following Suppression Assay: T cells isolated from 30 mL of PBMC...
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báo cáo hóa học:" Negative enrichment by immunomagnetic nanobeads for unbiased characterization of circulating tumor cells from peripheral blood of cancer patients" docx
Ngày tải lên :
20/06/2014, 03:20
... generation of cells with properties of stem cells, and to the ability of tumor cells to enter the circulation and seed metastases EpCAM-CK double positive CTC might represent only a subpopulation of ... combination of the both methods, in comparison to sole CD45 depletion However, the purity remained in the order of 1% with all three methods To evaluate the specificity of the methods presence of EpCAM+CK+CD45-, ... median of the number of these cells was (range: 1-43)/10 mL blood EpCAM+CKCD45- cells were detected in only two cases The detection rate of EpCAM-CK+CD45- cells was significantly higher than of EpCAM+CK-CD45...
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báo cáo hóa học:" Development of the Japanese version of the Pediatric Quality of Life Inventory™ Brain Tumor Module" pot
Ngày tải lên :
20/06/2014, 16:20
... Quality of Life Outcomes 2010, 8:38 http://www.hqlo.com/content/8/1/38 Page of 14 Table 4: Factorial validity of the Japanese version of the PedsQL™ Brain Tumor Module Child-report (n = 137, 64% of ... specificity of 0.95 and a power of 0.8, allowing for observation of ICC values of 0.5 or greater for ICC parameters of 0.8 [28] Ethical considerations This study was approved by the review boards of all ... Quality of Life Outcomes 2010, 8:38 http://www.hqlo.com/content/8/1/38 Page of 14 Table 2: Score distributions and child-parent relationships of the Japanese version of the PedsQL™ Brain Tumor...
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Báo cáo hóa học: "Research Article A Method for Visualizing Independent Spatio-Temporal Patterns of Brain Activity" potx
Ngày tải lên :
21/06/2014, 19:20
... addition of ICA produces a set of independent spatio-temporal patterns that are informative in terms of substantiating the morphology of the EEG during different mental states The Method of Common ... McFarland, “Control of a twodimensional movement signal by a noninvasive braincomputer interface in humans,” Proceedings of the National Academy of Sciences of the United States of America, vol 101, ... spatio-temporal morphologies indicative of the different mental states without prior knowledge of the SMR characteristics As with any application of ICA where exact number of sources is not known, it is...
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Báo cáo khoa học: "Changes of gastrointestinal argyrophil endocrine cells in the COLO205 tumor-implanted Balb/c-nu/nu mice" pps
Ngày tải lên :
07/08/2014, 18:21
... tcart IG eht ni sllec enircodne lihporygrA lanoiger eht gnivresbo roF tcart IG fo noiger hcae fo erutcetihcra lamron gnimrifnoc rof niats nisoe-nilyxotameh htiw deniats dna ,)mµ 4~3( denoitces ,niffarap ... sllec lihporygra IG fo sesaerced eht ,eroferehT ]61,51[ sisoropoetso lasuaponemtsop htiw stneitap ni rucco taht sdipil dna muiclac fo noitprosbalam eht rof elbisnopser eb yam sllec enircodne lihporygra ... tnereffid ,tcart IG eht fo noitacol eht ot gnidroccA snoiger dnalg lanitsetni dna cirtsag eht ni dnuof osla erew sllec )llec epyt esolc( epahs ni dnuor yllanoisacco elihw ,)epyt nepo( epahs ni eldnips...
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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt
Ngày tải lên :
09/08/2014, 07:20
... origin of these cells (Figure 2a– c) Moreover, complementary staining with MPO, CD20, CD3, CD45 and smooth muscle actin excluded the possibility of contamination with myeloid cells, B cells, T cells ... with RA [45] Our results agree with findings of the absence of a major role of TNF-α in the pathogenesis of pSS, as illustrated by the lack of efficacy of TNF-α blockers in this disease [48] However, ... capacity of SGECs to express and secrete BAFF after stimulation by IFN This peculiar property of epithelial cells is enhanced in patients with pSS and confirms the importance of resident cells of target...
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báo cáo khoa học: "Low-level expression of HER2 and CK19 in normal peripheral blood mononuclear cells: relevance for detection of circulating tumor cells" doc
Ngày tải lên :
10/08/2014, 22:20
... of Hematology & Oncology 2008, 1:2 http://www.jhoonline.org/content/1/1/2 Background Materials and methods The presence of circulating tumor cells (CTC) in peripheral blood and disseminated tumor ... origin of background and developing methods to selectively eliminate it is a critical step to improving the specificity of the RT-PCR method The goal of this study is to identify the source of background ... the specificity of the established method to detect CTC by identifying the source of the background signals and reducing them by the proposed method of negative immunoselection Our method was successful...
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optimization of protein and rna detection methodologies and a new approach for manipulating protein activity in living cells
Ngày tải lên :
13/11/2014, 10:46
... xii ABSTRACT OF THE DISSERATION Optimization of protein and RNA detection methodologies and a new approach for manipulating protein activity in living cells by Brent R Martin Doctor of Philosophy ... phenol/chloroform, precipitated with ethanol An optimized concentration of cassette was ligated with 15 µg of vector, extracted with phenol/chloroform, and precipitated with ethanol in the presence of ... oligomerization and raft localization of Ebola virus protein VP40 during vesicular budding Proceedings of the National Academy of Sciences of the United States of America 100, 15936-15941 (2003)...
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Báo cáo y học: "A Comparison of Immuncapture Agglutination and ELISA Methods in Serological Diagnosis of Brucellosis"
Ngày tải lên :
25/10/2012, 10:56
... Sci 2011, organism Isolation of the microorganism from the culture ensures diagnosis of the disease but sensitivity of this method is correlated 30-90 % with the stage of the disease (1) When the ... type of antibody (3) Obtaining negative results in agglutination tests is a common phenomenon One of the reasons for this is blocking antibodies One of the methods used to show existence of blocking ... Comparison of serologic tests which are used in the diagnosis of Brucellosis Mikrobiol Bült 2005;39:291-9 12 Alışkan H Diagnostic value of Culture and serological methods in human brucellsis Mikrobiol...
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Báo cáo y học: " Mutation Analysis of hCDC4 in AML Cells Identifies a New Intronic Polymorphis"
Ngày tải lên :
31/10/2012, 16:49
... variants (TV) of exon of the hCDC4 gene Arrows mark the position of the detected mutation in the 5’ UTR of Exon 1-TV3 and the new intronic SNP downstream of exon 10 (B) Chromatograph of the mutation ... of the hCDC4 gene may not play a pivotal role in the pathogenesis of AML This supplements studies which have discovered hCDC4 mutations in subsets of solid tumors and implies that mutations of ... of interest exists References 10 11 12 13 14 15 16 17 the Conflicts of interest Minella AC, Clurman BE Mechanisms of tumor suppression by the SCF(Fbw7) Cell Cycle 2005;4(10):1356-9 Fuchs SY Tumor...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization of tubulin in brain and testes: a comparison among some species of vertebrates Comp Biochem Physiol ... CY, Tan S, Chan WK, Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC...
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Báo cáo y học: "ene expression analysis of human red blood cells"
Ngày tải lên :
03/11/2012, 10:58
... (Table 1) Table The purity of human RBCs fraction tested by independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* ... classification of the resulting genes the gene ontology browser (Netaffx, Affymetrix, USA) was used Results and discussion Characterization of total RNA of human RBCs The purity of erythrocyte ... proposed A part of human erythrocytic proteins were identified 6, 7, 8, The number of genomic studies of RBCs is at present limited and represented either by micronucleus assay as a marker of classification...
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PHÂN TÍCH DAO ĐỘNG HỆ TRỤC CHÍNH TÀU THỦY BẰNG PHƯƠNG PHÁP PHẦN TỬ HỮU HẠN * VIBRATION ANALYSIS OF PROPULSION SYSTEM OF SHIP BY FINITE ELEMENT METHOD
Ngày tải lên :
09/04/2013, 12:58
... William T.Thomson: Theory of Vibration with Applications, Prentice Hall, 1982 M Géradin/D.Rixen: Mechanical Vibrations, Wiley, 1994 C.T.Sun, Y.P.LU: Vibration Damping of Structural Elements, Prentice ... Fundamentals of Mechanical Vibration, NJ, 2001 J S Carlton: Marine Propellers and Propulsion System, Bulterworth Heineman, 1998 Loyd's Register: "Rules and Regulations for The Classification of Ships",...
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