crosstalk between vegf and bcl 2 in tumor progression and angiogenesis

... X protein BCL- 2- antagonist ⁄ killer-1 BCL2 -related ovarian killer BCL- 2 antagonist of cell death BH3-interacting domain death agonist BCL- 2- like-11 BCL- 2- modifying factor BCL- 2- interacting killer ... current opinions on how Bcl- 2 and Bcl- xL are involved in the molecular events The crosstalk between the autophagy and apoptosis may redefine the roles of Bcl- 2 and Bcl- xL in oncogenesis and tumor progression ... domains or small molecules that mimic BH3 domains can bind to the BH3 receptor domain of Bcl- 2 or Bcl- xl, to disrupt the interaction between Bcl- 2 or Bcl- xl and Beclin In addition, Bcl- 2 ⁄ Bcl- xL...

Ngày tải lên: 28/03/2014, 23:20

... and death homeostasis is the key to the onset and maintenance of the transformed phenotype 1 .2 Bcl- 2 and Bcl- 2 family proteins: Since the discovery of Bcl- 2, many other Bcl- 2- like proteins ... to Bcl- 2 interaction with Nur77, a highly novel function of Bcl- 2 was reported Interaction of Bcl- 2 with Nur77 led to a conformational change in Bcl- 2, exposing its BH3 domain, converting Bcl- 2 ... binding and inhibition of anti-apoptotic Bcl- 2 proteins or the direct activation of Bax and Bak They also exhibit varying specificities in their binding to other Bcl- 2 family members (Willis and...

Ngày tải lên: 14/09/2015, 08:42

... have indicated that MVP is tyrosine phosphorylated in vivo in CHO and PC 12 cells and phosphorylated by PKC and casein kinase II in vitro using specific kinase agonist and inhibitors [31, 32] Although ... 27 4, 327 12 327 17 11 Kickhoefer VA, Poderycki MJ, Chan EKI & Rome LH (20 02) The La RNA binding protein interacts with the vault RNA and is a vault associated protein J Biol Chem 27 7, 4 125 2–4 126 8 ... that inactivate c-Src Recently Caveolin, a 22 kDa integral membrane protein [24 26 ] and a receptor for activated C kinase (RACK1) [27 ] were 794 shown to bind Src and suppress its tyrosine kinase...

Ngày tải lên: 07/03/2014, 12:20

... carboxylic acidsdecrease humangingival keratinocyte proliferation and increase apoptosis and necrosis J Clin Periodontol 1999, 25 :311-315 23 24 25 26 27 28 29 30 31 32 33 34 35 Kurita-Ochiai , Fukushima ... competing interests 20 21 22 Authors' contributions SB concieved and coordinated the study and participated in the collection of sample and data; and writing the manuscript HU participated in the ... 40 20 40 Bcl- 2 grade Bcl- 2 grade Bcl- 2 grade 25 75 * - 60 40 - * Significant difference (P < 0.05) active caspase-3, Fas, FasL and p53 in the inflammatory infiltrates was only observed in the inflammatory...

Ngày tải lên: 11/08/2014, 23:22

... monocyte-chemoattractant protein-1/ccl2 and interleukin-8/CXCL8 in endothelial cells during angiogenesis. " J Biol Chem 28 0 (2) : 129 2- 129 8 Garcia-Garcia, E and C Rosales (20 02) "Signal transduction receptor-mediated ... normal and infection-inflammation conditions are: TBS buffer containing 25 mM Tris, 145 mM NaCl, pH 7.4 and 2. 5 mM CaCl2 and MBS buffer containing 25 mM MES, 145 mM NaCl, pH 6.5 and mM CaCl2 [43,44] ... "Molecular interfaces of the galactose-binding protein Tectonin domains in host-pathogen interaction." J Biol Chem 28 5(13): 9898-9907 Lu, J and Y Le (1998) "Ficolins and the fibrinogen-like domain."...

Ngày tải lên: 11/09/2015, 09:57

... VEGF C and facilitates tumor lymphangiogenesis Int J Cancer 123 , 25 66 25 73 Alitalo K & Carmeliet P (20 02) Molecular mechanisms of lymphangiogenesis in health and disease Cancer Cell 1, 21 9 22 7 ... been established clinically [23 ,25 ,31] and in several in vitro and in vivo model systems, including wound healing [29 ,57], tumor xenografts [ 52, 55], Matrigel plug assay [57] and tube-like structure ... can reinfiltrate and promote growth and angiogenesis of the primary tumor [1 02] The possible involvement of heparanase in tumor selfseeding is supported by the timing of its induction during tumorigenesis...

Ngày tải lên: 15/03/2014, 23:20

... survival genes 28 2 Chang-Jong Moon, Yong-Duk Lee and Tae-Kyun Shin Fig Immunostaining for Bcl- 2 (A, B) in EAE Intense Bcl- 2 immunoreactivity is found in neurons and glial cells in the spinal cords ... cuffing, rather than in those in the parenchyma, as well as in some neurons and glial cells (Fig 2B) Table summarizes the expression of Bcl- 2 by cell phenotype The intensity of Bcl- 2 staining in ... diaminobenzidine- H2O2 solution (0.001% 3,3′ -diaminobenzidine [Sigma] and 0.01% H2O2 in 0.05 M Tris-buffered saline [TBS, pH 7.4]) The sections were counterstained with hematoxylin before mounting...

Ngày tải lên: 07/08/2014, 15:20

... disseminated cancer cells by 80% (reduction seen in primary tumor) Therefore (4) 𝑘𝑘 𝑑𝑑 = 0 .2 𝑘 𝑐𝑐 By combining (2) , (3) and (4), one finds (5) 𝐷𝐷𝐷𝐷𝐷𝐷 𝑐𝑐 � 𝐷𝐷𝐷𝐷𝐷𝐷 = 𝑑𝑑 Therefore, even an 80% inhibition ... chemokine/cytokine expression Differential expression of chemokines and cytokines in primary tumors and metastases Gene expression was measured by low density qRT-PCR arrays (except for CXCL2 which ... Alternate inhibitors of HGFR, EGFR and TGF-βR1 Alternative Inhibitors block PMN-MDSC induced EMT Alternative inhibitors block PMN-MDSC induced EMT in NBT-II cells AG1478 – EGFR inhibitor, SGX 523 –...

Ngày tải lên: 10/09/2015, 15:50

... the interaction between CRP and rL-FBG 12 14 20 21 27 32 35 39 44 45 79 80 82 84 85 91 92 94 95 98 99 1 02 x 3.8 3.9 3.10 3.11 3. 12 3.13 3.14 3.15 3.16 3.17 3.18 3.19 3 .20 3 .21 3 .22 3 .23 3 .24 3 .25 ... mutating Lys 25 9 into Ala in M-ficolin Reverse site-directed mutagenesis primer for mutating Lys 25 9 into Ala in M-ficolin Forward site-directed mutagenesis primer for mutating Phe 25 9 into Ala in ... tridentatus TPL -2 is an LPS binding protein and that the LPS-bound TPL -2 may serve as a platform to recruit the galactose binding protein (GBP) (Chen et al 20 01) Most surprisingly was our finding that...

Ngày tải lên: 11/09/2015, 09:57

... and spleen of tumorbearing mice were stained with CFSE (Invitrogen, C34554) Tumors cells and PMN25 MDSCs were treated 1h before and during the migration assay with inhibitors SB 225 0 02 and SB265610 ... to TAMs in other parts of the tumor [8,30] In summary, inflammation and the immune system play critical roles in both tumor suppression and tumor progression Understanding the complex interactions ... a normalizing control For IF staining of cells in culture, actin fibres were stained with Alexa Fluor 488 phalloidin (Invitrogen, A 123 79) and E-Cadherin was stained with anti-E-Cadherin Alexa...

Ngày tải lên: 10/09/2015, 15:50

... phosphatidylinositol 3-kinaseinteracting protein [11], plays a role in promoting autophagy [ 12] Beclin contains a Bcl- 2- binding domain which may serve as a point of cross-talk between the autophagic and apoptotic ... domain in the Bcl- 2- binding domain of Beclin was shown to bind to Bcl- XL [13] Anti-apoptotic Bcl- 2 and Bcl- XL have been shown to activate the autophagic response during programmed cell death in ... to determine the effect of Beclin and its mutant lacking the Bcl- 2- binding domain (Beclin 1DBcl2BD) [14] on autophagic activity under high- and low-nutrient conditions Both Flag–Beclin and its...

Ngày tải lên: 07/03/2014, 09:20

... Ste 12 Ste 12 v2 Ubi Ste11 PPP c1 Ste 12 Kss1 c23 c19 c1 c 22 Tec1 PREP c20 Ste 12 v25 v2 c1 Fus3 P P c9 v30 P Kss1 v2 v23 v28 c2 Tec1 v21 v 22 v18 Kss1 c 12 Ste 12 Ste 12 v1 Ste11 v7 v8 c9 Kss1 Ste 12 Kss1 ... 3 520 –3533 ª 20 06 The Authors Journal compilation ª 20 06 FEBS 3531 Modelling dynamic crosstalk in cell signalling 13 14 15 16 17 18 19 20 21 22 23 24 25 J Schaber et al the Gbeta binding domain of the ... mass-balanced signaling networks: a framework to obtain network properties including crosstalk J Theor Biol 22 7, 28 3 29 7 Binder B & Heinrich R (20 04) Interrelations between dynamical properties and structural...

Ngày tải lên: 30/03/2014, 10:20

... target Bcl- 2 family proteins is warranted The feasibility of measuring Bcl- 2 protein in a cooperative group setting is hypothesis generating and supports further study as a marker for Bcl- 2 targeted ... contrast, resulted in clinically meaningful decline in QOL, warranting the study of other novel agents that target Bcl- 2 family proteins Effects of therapy on PBMC Bcl- 2 protein supports the feasibility ... recently, our group and other investigators have studied novel BH3 domain mimetics such as AT101 and ABT263, which modulate multiple Bcl- 2 family proteins, and are being tested in early clinical studies...

Ngày tải lên: 18/06/2014, 16:20

... Curcumin( M) Bcl- 2 GAPDH C Curcumin( M) 40 60 93 .25 6.47 97.31 80 37.65 19.14 23 .86 62. 33 80. 82 92. 88 72. 29 49.91 38 .27 KG1a Red 76.13 Kasumi-1 27 .68 50.05 84.76 20 .25 7 .25 0.36 15 .22 79.73 92. 74 ... signaling pathways [21 ], including the mitogen-activated protein kinase [22 ], nuclear factor kappaB [23 ], phosphoinositide-3 kinase/Akt/mammalian target of rapamycin [24 ,25 ], Wnt [26 ], and Notchmediated ... examined using Wright-Giemsa, Hoechst 333 42 and Annexin-V/PI staining Both Wright-Giemsa and Hoechst 333 42 staining showed that curcumin induced morphological changes such as cell shrinkage and...

Ngày tải lên: 18/06/2014, 19:20

... alone Bcl- 2 protein is expressed in most tissue but it is overexpressed in tumors [54] The ability of G3139 to distribute in organs and to decrease Bcl- 2 protein level has been observed in clinical ... Hollenstein U, Wolff K, Pehamberger H: Chemosensitisation 22 23 24 25 26 27 28 29 of malignant melanoma by BCL2 antisense therapy Lancet 20 00, 356:1 728 -1733 Bedikian AY, Millward M, Pehamberger H, Conry ... blot analysis of bcl- 2 protein quantified and normalized to b-actin protein amount Panel B: representative immunohistochemical analysis of bcl- 2 protein in tumor sections (original magnification,...

Ngày tải lên: 18/06/2014, 22:20

... alone Bcl- 2 protein is expressed in most tissue but it is overexpressed in tumors [54] The ability of G3139 to distribute in organs and to decrease Bcl- 2 protein level has been observed in clinical ... Hollenstein U, Wolff K, Pehamberger H: Chemosensitisation 22 23 24 25 26 27 28 29 of malignant melanoma by BCL2 antisense therapy Lancet 20 00, 356:1 728 -1733 Bedikian AY, Millward M, Pehamberger H, Conry ... blot analysis of bcl- 2 protein quantified and normalized to b-actin protein amount Panel B: representative immunohistochemical analysis of bcl- 2 protein in tumor sections (original magnification,...

Ngày tải lên: 20/06/2014, 04:20

... cocaine on Bax and Bcl- 2 protein levels To determine whether cocaine leads to changes in Bcl- 2 family protein levels in fetal brain, we examined the Bcl- 2 and Bax protein expression As shown in ... upon cocaine insult Previous study [40] found that total Bcl- 2 protein is increased in injured brain after traumatic brain injury Neurons are resistant to ischemic injury when Bcl- 2 protein is over-expressed ... mitogen-activated protein kinase and mitochondrial/cytochrome c pathways J Pharmacol Exp Ther 20 05, 3 12: 1 12- 119 24 25 26 27 28 29 Xiao Y, He J, Gilbert RD, Zhang L Cocaine induces apoptosis in fetal myocardial...

Ngày tải lên: 08/08/2014, 17:20

... aggregate Fc␥RIIB in cells sensitized with 2. 4G2 rat Ab, and (as a consequence) coaggregate Fc⑀RI and Fc␥RIIB in cells sensitized with both IgE and 2. 4G2 rat Ab Since aggregation using 2. 4G2 rat Ab together ... gamma-chain J Immunol 1997;158:1803–9 24 Hemmings BA Akt signaling: linking membrane events to life and death decisions Science 1997 ;27 5: 628 –30 25 Coffer PJ, Jin J, Woodgett JR Protein kinase B ... phosphorylation and expression of forkhead and Bcl- 2 family members Scand J Immunol 20 06;63:1–6 28 Shinjyo T, Kuribara R, Inukai T, et al Downregulation of Bim, a proapoptotic relative of Bcl2 , is a...

Ngày tải lên: 08/08/2014, 21:20

... (As2O3) in endometrial carcinoma cells Gynecol Oncol 20 07, 105(1) :21 8 -22 2 Kang MH, Reynolds CP: Bcl- 2 inhibitors: targeting mitochondrial apoptotic pathways in cancer therapy Clin Cancer Res 20 09, ... signaling and NF-κB activities [14-17 ,28 ] Interestingly, the apoptotic effect of arsenic trioxide largely depends on a Bcl- 2- controlled pathway [ 12, 18 -20 ] Bcl- 2, an anti-apoptotic Bcl- 2 family ... Hospital of Wenzhou Medical College, Wenzhou 325 000, China 18 20 21 22 Received: December 20 09 Accepted: 20 April 20 10 Published: 20 April 20 10 23 © 20 10 ZhengofAccess articleBioMed Central Ltd...

Ngày tải lên: 09/08/2014, 03:21