criticisms of the cancer stem cell hypothesis

báo cáo khoa học: " LgR5 expression and cancer stem cell hypothesis: clue to define the true origin of esophageal adenocarcinomas with and without Barrett’s Esophagus?" ppsx

... by a cancer stem cell compartment [8] The existence of cancer stem cells would explain why only a small minority of cancer cells is capable of extensive proliferation within the tumor Furthermore, ... 5% of BE According to our hypothesis, that the intestinal stem cell marker LgR5 might also be suited to identify cancer stem cells, these might be the actively cycling Barrett (cancer) stem cells ... model of the CSC hypothesis and the clonal evolution theory [8] Further investigations are required to substantiate these findings Page 10 of 11 Acknowledgements The authors thank the assistance of...

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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt

Danh mục: Hóa học - Dầu khí

... cancers [1] Few of these markers were used for the confirmation of an MUC4 enriched cancer stem cell population Stem cells and cancer stem cells are known to possess the phenomenal property of ... overexpressed ovarian cancer cells induce the aggressiveness of the cancer cells and enrich the cancer stem cell population It also showed that MUC4 is expressed in the ovarian cancer stem cell population ... (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells would also be the targets of various immunotherapy...

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báo cáo hóa học:" Targeting the osteosarcoma cancer stem cell" pot

Danh mục: Hóa học - Dầu khí

... the bulk of the tumor [14-16] They are similar to stem cells and may arise from the transformation of stem cells or the de-differentiation of non -stem cells They are quiescent and capable of both ... Hypothesis The cancer stem cell hypothesis proposes that within a heterogeneous tumor there is a small subpopulation of cells called cancer stem cells (CSCs)” that are responsible for forming the ... to cancer therapies [15] Therefore, although treatment with chemotherapy or radiation may reduce the bulk of the tumor, it may actually miss the most important cell to target, the cancer stem cell...

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báo cáo hóa học:" Targeting the osteosarcoma cancer stem cell" ppt

Danh mục: Hóa học - Dầu khí

... the bulk of the tumor [14-16] They are similar to stem cells and may arise from the transformation of stem cells or the de-differentiation of non -stem cells They are quiescent and capable of both ... Hypothesis The cancer stem cell hypothesis proposes that within a heterogeneous tumor there is a small subpopulation of cells called cancer stem cells (CSCs)” that are responsible for forming the ... to cancer therapies [15] Therefore, although treatment with chemotherapy or radiation may reduce the bulk of the tumor, it may actually miss the most important cell to target, the cancer stem cell...

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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps

Danh mục: Báo cáo khoa học

... demonstrated both cancer cell and stem cell characteristics of the transformed P63+ basal /stem cells, supporting that they could be the origin of PCSCs under the influence of persistent Hedgehog ... prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate cancer Eur ... in the P63+ basal/ stem cells Patch1 and Smo were found highly expressed in the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells...

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Mechanisms of neurodegeneration and stem cell migration a study of molecular signals in the model of axotomy

Danh mục: Cao đẳng - Đại học

... characterization of the cells isolated from the SVZ of the adult rat brain 136 3.1.1 Morphology of the cells …………………………………………………136 3.1.2 The differentiation of the cells in vitro ... 6.2.5 Roles of chemokines in the migration of neural stem or progenitor cells 54 Aims of the present study…………………………………………………………… 55 7.1 Molecular analysis of the degeneration of the vagal motoneurons ... neural stem or progenitor cells in the SVZ………… 50 6.2.3 Migration of endogenous neural stem or progenitor cells……………… 51 6.2.3.1 Migration of endogenous neural stem or progenitor cells in the SVZ………...

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Clinical significance of cancer stem cell markers and other related proteins in colorectal carcinomas biological and methodological considerations

Danh mục: Tổng hợp

... asymmetric division in stem cells The mutated cancer stem cells then differentiate into other committed cancer daughter cells This cancer daughter cell harbours a combination of other mutations ... 2000) With the current awareness of the cancer stem- cell concept, it is of importance to examine the prognostic and predictive effects of these cancer stem- cell markers in colorectal cancers Currently, ... aspects of our hypothesis in determining clinical significance of cancer stem- cell marks in colorectal cancer Our main objective is to understand the clinical value of cancer stem- cell markers and their...

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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

Danh mục: Báo cáo khoa học

... indicated at the bottom of (A) (B, D) The cleavage of a number of substrates by HC The names and sequences of the different substrates are indicated above the pictures of the gels The times of cleavage ... acid of Fig Analysis of cleavage speciﬁcity by the use of new types of recombinant protein substrate (A) The overall structure of the recombinant protein substrates used for analysis of the efﬁciency ... charge of their side chains Verifying the consensus sequence by the use of a new type of recombinant protein substrate In order to verify the results from the phage display analysis, a new type of...

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Báo cáo sinh học: "Cancer stem cell subsets and their relationship" pot

Danh mục: Hóa học - Dầu khí

... reported the isolation of a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like ... will focus on the origin and the fate of primary cancer stem cells Precancerous stem cells Based on current literature, primary cancer stem cells may be derived from precancerous stem cells Chen ... primary cancer stem cells initiate from; whether primary cancer stem cells are same in the same type of cancer among different patients; and how to distinguish cancer stem cells from normal stem cells...

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Báo cáo hóa học: " Impairment of the CD8+ T cell response in lungs following infection with human respiratory syncytial virus is specific to the anatomical site rather than the virus, antigen, or route of infection" pdf

Danh mục: Hóa học - Dầu khí

... day after the primary infection, the number of pulmonary CD8+ cells capable of secreting IFNγ was only 26% the number of tetramer+CD8+ cells In contrast, the number of splenic IFNγ+CD8+ cells was ... CTL from the lungs of Examples ofof individual mice Figure the spleens primary data of flow cytometry analysis of tetramer/pentamer+CD8+ and IFNγ+CD8+ cells from the lungs and Examples of primary ... opposed to CD8+ cells, not shown) We also expressed the number of IFNγ+CD8+ cells as a percentage of the number of tetramer/pentamer+CD8+ cells (Figure 2) The resulting values confirmed the previous...

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Báo cáo hóa học: " Characterization of the IFN-γ T-cell responses to immediate early antigens in humans with genital herpes" doc

Danh mục: Hóa học - Dầu khí

... important to evaluate the specificity of the T -cell response Identification of antigens recognized by the immune system is an important description of the character of the immune response Being ... bead-bound CD8 cells and 1717 for free CD8 cells indicating that the bead bound CD8 cells and the free CD8 cells generate the same response under these assay conditions The ability of CD8 cells to ... expansion of cells may alter the relative levels of the different CD8 cell populations, in vitro expansion of cells was not pursued in our studies in order to maintain the ability of the IFN-γ...

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báo cáo hóa học:" Cancer stem cell subsets and their relationships" pot

Danh mục: Hóa học - Dầu khí

... reported the isolation of a type of precancerous stem cells from dendritic cell- like leukemic mice and the establishment of this precancerous stem cell line The precancerous stem cells had stem cell- like ... will focus on the origin and the fate of primary cancer stem cells Precancerous stem cells Based on current literature, primary cancer stem cells may be derived from precancerous stem cells Chen ... primary cancer stem cells initiate from; whether primary cancer stem cells are same in the same type of cancer among different patients; and how to distinguish cancer stem cells from normal stem cells...

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Báo cáo toán học: " Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" doc

Danh mục: Toán học

... for both cell lines This unique phenomenon could be due to a number of factors, either the presence of the MNPs both intracellular and on the membrane of the cells or the nanoparticles themselves ... understanding of the degree of cytotoxicity of the MNPs, we also investigated the effect the polymer-coated nanoparticles had on the integrity of the cell membrane and oxidative stress the MNPs elicited ... of one further centrifugation step at the end of the MTT assay in order to lyse the cells and discard the cellular components and CNT particles could reduce interferences caused by the CNTs They...

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báo cáo hóa học:" Dilemmas in the reliable estimation of the in-vitro cell viability in magnetic nanoparticle engineering: which tests and what protocols?" potx

Danh mục: Hóa học - Dầu khí

... for both cell lines This unique phenomenon could be due to a number of factors, either the presence of the MNPs both intracellular and on the membrane of the cells or the nanoparticles themselves ... understanding of the degree of cytotoxicity of the MNPs, we also investigated the effect the polymer-coated nanoparticles had on the integrity of the cell membrane and oxidative stress the MNPs elicited ... of one further centrifugation step at the end of the MTT assay in order to lyse the cells and discard the cellular components and CNT particles could reduce interferences caused by the CNTs They...

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Báo cáo y học: " Reconstitution of the adult B cell repertoire after treatment with rituximab" pptx

Danh mục: Báo cáo khoa học

... nonquantitative nature of the bulk PCR approach employed by the investigators Furthermore, for most of the study total B cells were studied without differentiating specific B cell subsets This situation ... it is tempting to postulate that upon profound B cell depletion there could be a re-enactment of early B cell ontogeny and that the cells described in the report by Rouzière and coworkers [1] ... on the interplay and competition between these cells and newly generated B cells The growing availability of patients treated with B cell depletion should allow investigators to understand the...

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Báo cáo y học: " Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis unravels new processes of adipocyte development" ppt

Danh mục: Báo cáo khoa học

... development The observation that the mESC culture system might not only allow the generation of adipose stem cells, but also of their potential vascular niche, opens new avenues for the study of adipose ... about the TF family known to bind this motif, the conservation level that gave the best over-representation, the number of gene promoters displaying the motif in the cluster, the number of times the ... system might allow the development of both adipose cells and their potential vascular niche opens exciting perspectives for the prospective isolation and the biochemical characterization of these...

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báo cáo khoa học: " MicroRNAs involved in neoplastic transformation of liver cancer stem cells" pot

Danh mục: Báo cáo khoa học

... for cellular heterogeneity of cancer due to aberrant differentiation According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, ... SP cells are enriched for markers of HSCs To examine whether SP cells are enriched for characteristics of stem cells compared to the non-SP cells, we further characterized the SP cells from the ... carcinogenesis and differentiation of normal stem cells strongly suggest that miRNA may be involved in the transformation of normal stem cells into cancer stem cells Therefore, screening for differences...

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Báo cáo y học: "Liposarcoma cells with aldefluor and CD133 activity have a cancer stem cell potential" pot

Danh mục: Báo cáo khoa học

... negative, = less than 10% of the tumour cells scored positive, = 10-50% of the rumour cells scored positive, = more than 50% of the tumour cells scored positive *For one of the tumors, a de-differentiated ... percentage of the cells following culturing in RPMI The percentage of double-positive cells increased dramatically to approximately 40% due to an increasing number of cells expressing ALDH when the cells ... Aldefluor in combination with other stem cell markers improves the enrichment of CSCs [8,13,42] A likely cell of origin for the sarcoma-CSC is an MSC-like stem or progenitor cell However, since no markers...

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Báo cáo y học: "Transcriptomic and phenotypic analysis of murine embryonic stem cell derived BMP2+ lineage cells: an insight into mesodermal patterning" ppt

Danh mục: Báo cáo khoa học

... of the BMP2+ cells to cardiac cells Differentiation of the BMP2+ cells to cardiac cells (a) RT-PCR analysis of the cardiac markers in BMP2+ cells and other controls (b) Schematic outline of the ... more likely, the complete absence of the endodermal cell lineage The BMP2+ cell lineage contains neural crest stem cells and their derivatives The BMP2+ cells showed enriched expression of ectodermal ... differentiate into cells of the myofibres of skeletal muscles [78] An elevated level of MyoD indicates either the proliferation or the differentiation of satellite cells into primary myoblasts in the BMP2+...

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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx

Danh mục: Báo cáo khoa học

... diabetes or ischemia/reperfusion injury of the retina, they homed to the site of injury and showed robust reparative function of the damaged vasculature The cells also showed a similar regenerative ... assess the total amount of RNA in a population and can, therefore, be influenced by a predominating cell type Variations in the homogeneity of the population can influence the number of genes ... representative of the various cell types within the heterogeneous population of BCs We describe our method of assessing heterogeneous samples in three levels of analysis The first level consists of making...

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