creactive protein range in blood
Tài liệu Báo cáo khoa học: Transient RNA–protein interactions in RNA folding docx
Ngày tải lên :
14/02/2014, 19:20
... the properties of the three
protein classes, with the main focus being on RNA
chaperones and annealer proteins.
Specifically binding proteins
A specific protein cofactor binds to its RNA target
through ... mRNA-binding protein hnRNP A1 [61], the
Xenopus laevis protein X1rbpa [54], the trypanosome
guideRNA-binding protein RBP16 [62], and the
Escherichia coli protein StpA [63], an inverse or miss-
ing correlation ... easily gain access to the
RNA protein interface to increase the dissociation
process. The promiscuity often reported for proteins
M. Doetsch et al. Transient RNAprotein interactions in RNA folding
FEBS...
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Tài liệu Báo cáo khoa học: Cobalamin uptake and reactivation occurs through specific protein interactions in the methionine synthase–methionine synthase reductase complex docx
Ngày tải lên :
18/02/2014, 08:20
... 53–56.
34 Tollin G (1995) Use of flavin photochemistry to probe
intraprotein and interprotein electron transfer mecha-
nisms. J Bioenerg Biomembr 27, 303–309.
Supporting information
The following supplementary ... structure of the cobalamin-binding domain
Homocysteine
Methionine
Co
Methylcob(III)alamin
Cob(I)alamin
Primary
turnover
cycle
H
CH
3
H
4
folate
AdoHyc
Cob(I)alamin
Cob(II)alamin
H
4
folate
Co
NADPH
A
doMet
e
–
FAD
FMN
NADPH
NADP
+
e
–
Reactivation
Co
Fig. ... CH
3
-H
4
-folate binding-domain (black barrel) form discrete complexes with the cobalamin-binding domain (dark grey circle).
hMS is inactivated approximately every 200–1000 catalytic turnovers [owing to...
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Tài liệu Báo cáo khoa học: Identification of a novel matrix protein contained in a protein aggregate associated with collagen in fish otoliths pdf
Ngày tải lên :
18/02/2014, 17:20
... determine the calcium-binding domain of
the protein. (A) Schematic drawing of the recombinant proteins. Six
GST-fused recombinant proteins containing the three distinctive
domains of tandem repeat ... matrix proteins
induce aragonite crystals [4–7]. We therefore exam-
ined proteins contained in aggregates within the
otolith matrix and identified a protein contained in
the HMW protein glycosaminoglycan ... P
i
, and specific
binding of the antibody was detected by incubating the
sections in a solution of 10 mm diaminobenzidine in
10 mm NaCl ⁄ P
i
.
H. Tohse et al. A novel protein in the otolith matrix...
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Tài liệu Báo cáo khoa học: Bone morphogenetic protein signaling in stem cells ) one signal, many consequences pptx
Ngày tải lên :
19/02/2014, 00:20
... neuroepithelial
cells into astrocytes, by binding and hyper activating
the promoter of glial fibrillary acidic protein (GFAP).
In parallel, BMP signals lead to enhanced expression
of ID proteins, which in turn bind ... protein- induced
T. U. Wagner BMP signaling in stem cells
FEBS Journal 274 (2007) 29682976 ê 2007 The Author Journal compilation ª 2007 FEBS 2975
MINIREVIEW
Bone morphogenetic protein signaling ... of ligand-receptor affinity ) usually by
binding to ligand dimers
Dimeric secreted proteins such as noggin,
chordin, follistatin and GDF3 bind and therby
inactivate BMP dimers [16]
Cell membrane...
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Tài liệu Báo cáo khoa học: Altered inactivation pathway of factor Va by activated protein C in the presence of heparin doc
Ngày tải lên :
19/02/2014, 13:20
... structure of
heparin (file 1hpn [35]).
Amino acids known to be part of the heparin-binding site
in these three crystallized protein heparin complexes were
given as starting point for the docking search ... observation that
heparin does not inhibit FVa inactivation by recombinant
APC variants that are unable to bind heparin supports the
mechanism in which heparin binding to the basic cluster in
APC is responsible ... deficient in heparin binding
To verify that the inhibition of APC-mediated FVa
inactivation by heparin is related to the ability of heparin
to bind APC, we used recombinant mutants of APC
harboring...
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Tài liệu Báo cáo khoa học: Co-operative effect of the isoforms of type III antifreeze protein expressed in Notched-fin eelpout, Zoarces elongatus Kner ppt
Ngày tải lên :
19/02/2014, 16:20
... listed in
Fig. 4). Again both nfeAFP2 and nfeAFP6 were
expressed with C-terminal lysines. We also expressed
nfeAFP6minusLys66 (nfeAFP6DLys; SP group) to
examine the effect of the C-terminal lysine ... solution was placed in the
cooling pot ()7 °C) of the instrument; (b) the frosty probe
was put manually into the supercooled sample to initiate
water freezing; (c) the increase in solution temperature
caused ... (1996) A natural variant of type I antifreeze protein
with four ice-binding repeats is a particularly potent
antifreeze. Protein Sci 5, 1150–1156.
29 Leinala EK Davies PL Doucet D Tyshenko MG
Walker...
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Tài liệu Báo cáo Y học: Control of p70 ribosomal protein S6 kinase and acetyl-CoA carboxylase by AMP-activated protein kinase and protein phosphatases in isolated hepatocytes pot
Ngày tải lên :
22/02/2014, 07:20
... rapamycin;
p70S6K, p70 ribosomal protein S6 kinase; PDK1, 3-phosphoinositide-
dependent protein kinase; PKB, protein kinase B; PP2A, protein
phosphatase 2A.
*Present address: GlaxoSmithKline Biologicals,
Research ... shown
schematically in Fig. 8, based on the available data in the
literature and integrating the results obtained in the present
study.
ACC is known to be activated by incubating hepatocytes
with certain amino ... Hemmings, B.A. &
Thomas, G. (1999) Protein kinase B localization and activation
differentially affect S6 kinase 1 activity and eukaryotic translation
initiation factor 4E-binding protein 1...
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Báo cáo khoa học: Adipophilin protein expression in muscle – a possible protective role against insulin resistance pot
Ngày tải lên :
06/03/2014, 09:22
... the
present study indicate that adipophilin protein expression in muscle is
involved in maintaining insulin sensitivity.
Abbreviations
Adfp, adipophilin; CLB, classical lysis buffer; FA, fatty ... is implicated in
insulin resistance. As shown in the present study,
palmitic acid indeed impaired insulin signaling in
C2C12 cells, which is in line with numerous studies
addressing the effect ... in the glycolysis, were reduced in the palmitic
acid-treated cells. Additionally, the protein level of
prohibitin was increased in palmitic acid-treated cells.
Prohibitin is involved in the inhibition...
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Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt
Ngày tải lên :
07/03/2014, 00:20
... glycine, l-alanine,
l-proline, l-lysine, l-serine, b-alanine, taurine, sarcosine,
dimethylglycine, glycine betaine, trimethylamine N-oxide,
and cytidine 2Â-3Â cyclic monophosphate were also obtained
from ... stabilize proteins
[3,4], but they also induce refolding of misfolded
proteins [5–8] and remove protein aggregation [9–12].
Mechanisms of protein osmolyte interactions, the
effect of osmolytes on protein ... alanine, proline, serine, lysine, b-alanine and
taurine) that have no significant effects on both DG
D
°
and k
cat
. (b) Class II represents methylamines (sarco-
sine, dimethylglycine, betaine,...
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Báo cáo khoa học: Protein transport in organelles: The Toc complex way of preprotein import pdf
Ngày tải lên :
07/03/2014, 03:20
... contains three TPR motifs
forming the docking site for Hsp90-bound preproteins. AtToc12 (12)
exposes a J-domain (J) into the intermembrane space and has a
role in anchoring Hsp70, thereby assisting ... accumulation of many proteins involved in photosyn-
thesis (termed ‘photosynthetic proteins’), suggesting
that both atToc33 and atToc159 are involved in the
import of photosynthetic proteins. However, ... photosyn-
thetic preproteins (down-regulated in the mutants) is
unclear. However, many proteins that are not involved
in photosynthesis (termed ‘housekeeping proteins’)
accumulate normally in both ppi1...
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Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
Ngày tải lên :
07/03/2014, 03:20
... domains are degenerate 34-amino acid
repeats forming anti-parallel a-helices known to be
involved in an array of protein protein interactions
(generally with non-TPR proteins) [41]. Both domains
are ... calmodulin; ClpC, caseinolytic protease C; Cpn, chaperonin; FNR, ferredoxin-NADP
+
-oxidoreductase; Hip, Hsp70-interacting protein;
Hop, Hsp70/Hsp90-organizing protein; Hsp, heat shock protein; IEM, ... CaM-binding
domains, and contains at least one conserved potential
CaM-binding motif [46]. Additionally, the binding of
CaM at the C-terminus and the binding of NADPH at
the extreme N-terminus...
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Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx
Ngày tải lên :
07/03/2014, 03:20
... for proteinaceous insertion machinery. Other single-
spanning proteins have also been suggested to use this
membrane integration route, including the photosys-
tem II subunits, PsbW and PsbX. In ... thylakoid
transport of several proteins showed them to be protein
Keywords
protein transport; secretory pathway; SRP;
Tat; thylakoid; twin-arginine
Correspondence
C. Robinson, Department of Biological
Sciences, ... pro-
tein conducting channel. Cross-linking studies have
shown that Tha4 undergoes conformational rearrange-
ment during active protein transport, with the amphi-
pathic helix and C-terminal tail interacting...
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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
Ngày tải lên :
07/03/2014, 03:20
... chloroplastic
targeting signals in terms of arginine and serine con-
tent (i.e. not as high as in mitochondrial targeting
signals) and may be slightly enriched in hydrophobic
residues. In yeast, proteins targeted ... targeted in Medicago truncatula and
Populus alba without a cleavable N-terminal targeting
signal [30].
Mature proteins
Analysis of the role of the mature protein in dual target-
ing in several ... here
[17,18]. Instead, we provide an overview the signals,
proteins, receptors, sorting of dual-targeted proteins
and why dual targeting may occur. Finally, an outline
of the future challenges in this...
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Báo cáo khoa học: Fluorescence studies of the replication initiator protein RepA in complex with operator and iteron sequences and free in solution pdf
Ngày tải lên :
07/03/2014, 04:20
... DNA-binding domains involved in
binding), or 1DR-short (as a dimer, but presumably
with only one domain involved), or indeed when
bound as a monomer to 1DR. This suggests that bind-
ing to both inverted ... DNA-binding domains, and rearrangement of the
relative orientation of the two domains [7,9]. The con-
formational change upon iteron binding may expose a
recognition site for protein protein interaction,
enabling ... sequence. RepA contains two
domains, one of which binds specifically to the half-repeat. The other is the
dimerization domain, which is involved in protein protein interactions in
the dimeric RepA–operon...
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Báo cáo khoa học: Covalent activation of heart AMP-activated protein kinase in response to physiological concentrations of long-chain fatty acids docx
Ngày tải lên :
07/03/2014, 15:20
... Lopaschuk, G.D. (1997) Insulin inhibition of
5Â-adenosine monophosphate-activated protein kinase in the heart
results in activation of acetyl coenzyme A carboxylase and inhi-
bition of fatty acid ... AMP-
activated protein kinase kinase from rat liver and identification of
threonine 172 as the major site at which it phosphorylates AMP-
activated protein kinase. J. Biol. Chem. 271, 27879–27887.
54. Stein,S.C.,Woods,A.,Jones,N.A.,Davison,M.D.&Carling,D.
(2000) ... (1996) Characterization of
5ÂAMP-activated protein kinase activity in the heart and its role in
inhibiting acetyl-CoA carboxylase during reperfusion following
ischemia. Biochim. Biophys. Acta. 1301,...
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