corn and soy derived materials properties and potential clinical applications
Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx
... E-cadherin distribution, cell scattering and uPA expression (A) T47D cells (a and b) and MCF7 cells (c and d) were treated for h with control or Decma supernatant and immunostained with anti-E-cadherin ... (a and b) and MCF7 cells (c and d) were grown for days to 60–70% confluence and then treated with control or Decma supernatant for h before recording (C) MCF7 cells were treated with Decma and ... LY294002 and cytochalasin D (CytD) was from Sigma-Aldrich GmbH (Basel, Switzerland), SP600125 was obtained from Biomol (Wangen, Switzerland), and TPA, horseradish peroxidase-conjugated antimouse and...
- 14
- 599
- 0
Global orchestration of gene expression by the biological clock of cyanobacteria Carl Hirschie Johnson pot
... first reported the functional replacement of kaiBCp [15], and now Nakahira and coworkers [16] report the functional replacement of both kaiAp and kaiBCp Both studies found that expression of the Kai ... KaiA, KaiB, and KaiC are synthesized from the kaiABC cluster using two promoters: kaiAp (driving expression of KaiA) and kaiBCp (driving expression of a dicistronic mRNA encoding KaiB and KaiC) ... input and are clock-dominated, or high-amplitude (including kaiBCp), and the remaining 90% of promoters (clock-modulated, or low-amplitude; including kaiAp) receive both rhythmic input and basal...
- 4
- 247
- 0
Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx
... Decreased DNA binding and p65 protein level Decreased nuclear p50 and p65 levels and phosphorylated STAT1 and STAT5 Decreased IKKb and decreased p65 binding Increased IjBa expression and decreased p65 ... CHOP and Gadd 45 CHOP CHOP HIF-1a/2a, Gadd 34 and CHOP HIF-1a HSF-1 HSF-1 Reference Increased mRNA level Increased mRNA levels and increased c-jun activity Increased p53 phosphorylation and decreased ... mRNA level Decreased mRNA and protein levels Decreased HIF-1/2 a protein, increased Gadd 34 and CHOP mRNA levels Decreased protein level c-jun AP-1 (c-jun) and c-myc p53 and c-myc [123] [124] Increased...
- 19
- 361
- 0
Báo cáo khoa học: Dynamic association of MLL1, H3K4 trimethylation with chromatin and Hox gene expression during the cell cycle ppt
... E2F4 and E2F6 with different affinities, whereas MLL2 interacts with a different subset of E2Fs, such as E2F2, E2F3, E2F5 and E2F6 [26,35] Distinct interactions between E2Fs and MLLs suggest potential ... progression and maintenance of the cell cycle As MLLs and H3K4-specific methylations are well known to play critical roles in gene expression, we analyzed the dynamics and functions of MLLs and H3K4 ... of RNAP II and MLL1 recruitment and H3K4 trimethylation marks were observed at the beginning of the S phase (0 h), and these marks were attenuated for the rest of the cell cycle (10 and 20 h),...
- 12
- 439
- 0
báo cáo hóa học:" Progressive obesity leads to altered ovarian gene expression in the Lethal Yellow mouse: a microarray study" pptx
... 90 and 180-day black and LY mice (mean Body weight (g) of 90 and 180-day black and LY mice (mean ± SEM; n = for each group) relative expression, i.e agouti was 350-fold greater in LY mice and ... humans, cortisol and cortisone are the major active and inactive forms, respectively Glucocorticoids are important in the pathogenesis of obesity and insulin resistance, and expression and activity ... CH, and KT carried out the treatments and tissue collection, prepared preliminary data summaries, and participated in microarray analyses KE performed RNA extractions and microarray analyses, and...
- 9
- 346
- 0
Báo cáo sinh học: " Coordinated gene expression by post-transcriptional regulons in African trypanosomes" potx
... further into epimastigote and metacyclic forms within the insect The three recent BMC Genomics papers by Kabani and colleagues [1], Jensen and colleagues [2], and Queiroz and colleagues [3] have ... constitutive and that chromatin structure, in part mediated through histone modifications, will determine transcription start and termination sites These not seem to be sequencespecific and several ... between the bloodstream of mammalian hosts and the tsetse fly vector This cycling is accompanied by changes in morphology, in metabolism, and in RNA and protein expression Because the genome of...
- 4
- 326
- 0
Báo cáo khoa học: "Effects of Benzo[a]pyrene, 2-Bromopropane, Phenol and 2,3,7,8-Tetrachlorodibenzo-p-Dioxin on Proinflammatory Cytokines Gene Expression by Mice Spleen Cells" doc
... were: 30 cycles of 30 s at 94℃, 30 s at 60℃ and 90 s at 72℃ for β-actin, IL-1β and TNF α, 25cycles of 30 s at 94℃, 30 s at 56℃ and 30 s at 72℃ for IL-6 and IFNγ The sequences of primers used are ... splenocytes to 10-9M of TCDD induces IFNγ and TNFα gene expression, and suppresses IL-1 expression 10-6M phenol suppresses IL-1, IL-6 and TNFα gene expression, and 2-BP downregulates TNFα gene expression ... harvested and incubated with lysis buffer (50mM Tris-HCl, 20mM EDTA, 1% NP-40, pH 7.5) on ice for 30 min, and supernatants were obtained by centrifugation Supernatants were incubated with 1% SDS and...
- 8
- 420
- 0
Báo cáo khoa học: "Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks" ppsx
... ihsigeN ,J rehtnyG ,R erooM ,P iksokaknoH 21 191-971 ,8 ,9891 AND seneg tnereffid morf debircsnart sANRm 054P owt fo noitaluger :ANDc 054P emorhcotyc elbicudni latibrabonehp nekcihc a fo ecneuqeS ... fo emulov noitcaer lanif a ni tuo deirrac saw RCP slocotorp eht gnisu tuo deirrac saw sisehtnys ANDc dnarts tsriF ANR fo noitartnecnoc lµ/gµ htiw detcudnoc saw RCP-TR evitatitnauQ RCP-TR evitatitnauQ ... eht sa tes saw snoitidnoc gnilcycomrehT lm 52 fo emulov lanif eht ekam ot ebut hcae ni dedda saw ANDc fo elpmas lm eerhT ebut RCP hcae ni seititnauq lm 22 ni detouqila dna deraperp saw selpmas...
- 7
- 291
- 0
Báo cáo toán học: "Iodine Alters Gene Expression in the MCF7 Breast Cancer Cell Line: Evidence for an Anti-Estrogen Effect of Iodine" doc
... 30], DNA repair (GADD45A and DDIT4) [31, 32], cell cycle and proliferation (ASNS and GADD45A) [33, 34], tRNA synthesis (WARS, GARS, YARS) [35-37] and transcription (SQSTM1 and DSIPI) [38, 39] The ... cycle (LGALS1, UBE2C, TYMS, MYBL2, and CCND1) [19-25], cell growth/differentiation (GFRA1) [26], nucleotide synthesis (TK1 and TYMS) [27, 28], and ubiquination and cyclin destruction (UBE2C) [19] ... Materials for details) RNA was isolated and subjected to Microarray Analysis (see Methods and Materials for details) 29 genes were upregulated ≥ 2.0-fold (A) and 14 genes were down regulated ≥ 2.0-fold...
- 8
- 290
- 0
Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf
... of chemokines and their receptors [10–12] Cytokines and chemokines in pSS In patients with pSS the salivary and lacrimal glands are the main target organs for the immune system and, at least ... normal salivary glands It has therefore been suggested that T helper type (Th1) cytokines, such as IFN-γ and IL-2, as well as IL-10, IL-6 and TGF-β, might be important in the induction and/ or maintenance ... production and the strong association of certain autoantibodies with particular MHC class II alleles, and despite increasing knowledge about tissue B cell immunology and the role of cytokines and chemokines,...
- 12
- 351
- 0
Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... a set of standards representing mRNA acquired from between 24 and 100,000 cells Quantitative PCR Materials and methods Reagents All reagents required for reverse transcription PCR and Q-PCR were ... standards containing cDNA from the equivalent of 24–100,000 cells Standard curves were determined for IL-1β, IL-6, TNF-α, MMP-1, and GAPDH using TaqMan chemistry and Q-PCR as described in Materials ... Q-PCR using the standard curve method The CEs were determined based on the PBMC reference standard Table presents the CEs of GAPDH, IL-1β, IL-6 and MMP-1 with the standard deviation and percentage...
- 9
- 557
- 0
Báo cáo y học: "Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte" pptx
... The number of elongated cells per field of view (1.376 mm2) was counted and averaged Materials and methods RNA extraction and northern blot analysis Primary cell culture Total RNA was collected ... of aggrecan and type II collagen mRNA (89 ± 2% and 81 ± 7%, respectively; data not shown) The combination of TNF-α and EGF therefore produces an additive decrease in both aggrecan and type II ... mRNA by TNF-α and EGF TNF-α and EGF have been found to activate PKC in other cell types The role of PKC signaling in the reduction of aggrecan and type II collagen mRNA by TNF-α and EGF was examined...
- 12
- 388
- 0
Curcumin modulates dopaminergic receptor, CREB and phospholipase c gene expression in the cerebral cortex and cerebellum of streptozotocin induced diabetic rats potx
... 12 hour light and 12 hour dark periods Rats had free access to standard food and water ad libitum All animal care and procedures were done in accordance with the Institutional and National Institute ... forward primer, reverse primer and TaqMan probe for assay on demand and endogenous control β-actin and 12.5 μl of Taqman 2× Universal PCR Master Mix (Applied Biosystems) and the volume was made up ... effects The biological and chemical properties of curcuminoids were reported [12] A number of experimental studies have demonstrated CUR's antioxidant and neuroprotective potential [13,14] Also...
- 11
- 413
- 0
Báo cáo y học: "Regulation of chondrocyte gene expression by osteogenic protein-1" ppt
... Protein-1, and Fibroblast Growth Factor (FGF)-7 Their expression was inhibited by rhOP-1 from 1.93- to 1.5fold Contrary, the expression of the FGF-R2 and receptors, and a and b receptors of Platelet -Derived ... trehalose, and 0.1% Tween 20), 0.5X SYBR Green (vendor stock 10,000X; Cambrex, Rockland, ME), 0.2 μM each of forward and reverse primer (IDT primers) or 1X QuantiTect primers (Qiagen primers) and μl ... cytoskeleton (cyclin D, Talin1, and Cyclin M1, for example, and confirmed in [36]), matrix production and other anabolic pathways, as well as regulation of cytokines and various catabolic pathways...
- 14
- 312
- 0
Retrovirology Commentary BioMed Central Open Access Inhibition of HIV-1 gene expression by pptx
... correspond to unspliced (encoding Gag and Gagpol), singly spliced (to produce Vif, Vpr, Vpu and Env) or multiply spliced (for generating Tat, Rev and Nef) mRNAs Unspliced and singly spliced viral RNAs ... unspliced, singly spliced and nef mRNAs to block their translation by preventing the binding of PABP1 (shown as a small blue circle) In contrast, PABP1 binds to tat and rev mRNAs, and translation is ... reading frames and the influence of translation on 3' UTR structure/RNP composition Both tat and rev mRNAs contain reading frames encoding the respective proteins (Tat or Rev) and that of Nef,...
- 4
- 215
- 0
Báo cáo y học: " Inhibition of HIV-1 gene expression by Ciclopirox and Deferiprone, drugs that prevent hypusination of eukaryotic initiation factor 5A" potx
... (Dhp); and its subsequent hydroxylation, catalyzed by DOHH, yielding hypusine (Hpu) DHS is inhibited by GC7 and DOHH by CPX and DEF, as indicated B Structures of CPX, Agent P2, DEF and DFOX C CPX and ... be tested clinically for HIV-1 inhibition and could be developed as antiretrovirals Materials and methods Reagents Deferiprone was purchased from Calbiochem Ciclopirox, deferoxamine and actinomycin ... from nt 1506 to 5784 using SpeI and SalI enzymes Similarly, truncations IV (nt 5784 to 8464) and V (nt 712 to 8464) were made with SaII and BamHI and with BssHII and BamHI, respectively The plasmid...
- 17
- 353
- 0
Báo cáo y học: "Comment on and reply to "Analysis of variation of amplitudes in cell cycle gene expression" by Liu, Gaido and Wolfinger: On the analysis of gene expression during the normal, eukaryotic, cell cycle" docx
... content, which goes down to 2N and back to 4N and never repeats the 8N In between the start and the end there is no systematic variation of cells through G1, S and G2 In summary, the work of ... cell cycle, and during the period of cell division there should be a bimodal distribution of cell sizes One should apply clearly stated and stringent criteria for synchronization and microarray ... object model) standards, so that the public data can be analyzed 18) Microarray results should be compared to randomized data to show that the observed cyclicities are not the result of random noise...
- 5
- 266
- 0
Báo cáo y học: "Mouse maps of gene expression in the brain" pot
... user to several standard published adult and developmental brain atlases for both mice and rats The GENSAT project goes farthest in describing the anatomy of gene expression and compares data ... Atlas and GENSAT The GenePaint atlas [10] catalogs in situ hybridization expression data in selected sections of whole mouse embryos for several thousand genes at embryonic day (E) 14.5 and augments ... limitation to one age and one gender will no doubt be addressed in future efforts And, at this point, the annotation of the anatomical location of expression is only semi-automated and requires expert...
- 4
- 279
- 0
Báo cáo y học: "Cell-specific microarray profiling experiments reveal a comprehensive picture of gene expression in the C. elegans nervous system" ppsx
... plasma membrane and vesicular transporters for choline and acetylcholine (cho-1 and unc-17), GABA (snf-11 and unc-46, unc-47), dopamine (dat-1 and cat-1), and glutamate (glt-3 and eat-4) are included ... plots and R2 values for pairwise combinations of the EP and LA datasets Additional data file lists 27 uncharacterized human homologues in the LP and EP datasets, and indicates mouse homolog and ... (arrow) and in specific ventral cord motor neurons (e) at the L2 stage (b,c) tsp-7::GFP and C04E12.7::GFP are widely expressed in the nervous system with bright GFP in head and tail ganglia and in...
- 32
- 265
- 0
Báo cáo y học: "The temporal program of peripheral blood gene expression in the response of nonhuman primates to Ebola hemorrhagic fever" ppsx
... and MX2, IFN-γ inducible protein-10, 2'-5' oligoadenylate synthetase-1, -2, and -3, guanylate binding protein-1 and -2, signal transducer and activators of transcription (STAT)-1, double-stranded ... response, Fos, Jun) and IFN responsive genes (MX1 and 2, STAT-1, IFN-γ inducible protein-10, guanylate binding protein-1 and -2) Animals had no detectable clinical illness at days and 2, were feeding ... Animal Welfare Act and other federal statues and regulations relating to animals and experiments involving animals, and adheres to the principles stated in the Guide for the Care and Use of Laboratory...
- 14
- 601
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose