construction of yeast surface displayed cdna libraries

... synthesis of even longer full-length cDNAs, and higher representation of long full-length cDNAs in the library In summary, this method of selecting full-length cDNAs by biotinylation of the mRNA ... terms of SSRs varied with RepeatFinder identifying only 175 in total (2.5 % of ESTs) but SciRoKo finding a total of 1,932 (24.3% of ESTs) 21 Discussion The major success of this research was the construction ... was the construction of two full-length cDNA libraries from two important common bean genotypes grown under three types of abiotic stress and the preliminary sequencing of the libraries with approximately...

Ngày tải lên: 11/08/2014, 11:21

... protein–protein interactions A B C Fig Schematic outline of gene construction (A) Structural features of the yeast endogenous Gc gene (STE18), and design of the Gc–Fc fusion gene excluding the lipidation ... compilation ª 2009 FEBS N Fukuda et al Construction of yeast strains containing Zmem genes rather than the ZZmem gene were achieved using a process similar to construction of the BZG2118 strain, with the ... budding yeast Nucleic Acids Res 24, 2519–2524 Nakamura Y, Shibasaki S, Ueda M, Tanaka A, Fukuda H & Kondo A (2001) Development of novel wholecell immunoadsorbents by yeast surface display of the...

Ngày tải lên: 16/03/2014, 01:20

... Stage of RACE Prepare the template by diluting the aliquot of nonamplified cDNA fivefold in the cDNA dilution buffer If the use of amplified cDNA is feasible, dilute the aliquot of the product of cDNA ... equal volume of phenolϺchloroform RNA is precipitated with ethanol, air-dried, dissolved with H2O, and stored in –20°C; it can be used for construction of cDNA libraries Regeneration of cDNA Oligotex ... protein libraries are collections of gene products of cells or tissues 2.3 Why cDNA Libraries? Complementary DNA libraries are preferable to mRNA libraries for the following reasons: cDNA can...

Ngày tải lên: 11/04/2014, 01:04

... of DNA are: Duty Cycle of 5; Intensity of 5; Cycle per Burst of 500; Seconds of 120; Well Plate of '96 well offset + mm' It is important to avoid droplets being splashed and held at the top of ... plate-based acoustic shearing of genomic DNA Figure Robust, optimized plate-based acoustic shearing of genomic DNA (a) Effect of time on shearing profile Agilent Bioanalyzer traces of μg human genomic ... of multiplexing for small-to-medium numbers of samples (for example, 20 to 40 libraries) In this method, plate-based library construction proceeds to completion as described above Completed libraries...

Ngày tải lên: 09/08/2014, 20:21

... input of NA12878 genomic DNA, in triplicate Bars: total DNA output from pond library construction before PCR amplification Blue diamonds: percentage recovery of input DNA for duplicates of μg of ... place or from running the wrong combination of program and deck configuration Quantification of pond libraries and catch libraries Prior to selection, pond libraries are assayed for concentration ... complexity of the original manual process, delivering selected sequence reads with a high on-target rate of > 83%, and a median rate of duplicated reads of approximately 4%, similar to that of whole...

Ngày tải lên: 09/08/2014, 22:23

... during the development of guar seeds Results and Discussion Generation of cDNA libraries Figure shows sections of developing guar seeds at 25 days after flowering (DAF) and of mature seeds at 40 ... fertilized at time of watering using a commercial fertilizer mix (Peters Professional 20-10-20 (N-P-K) General Purpose, The Scotts Company, Marysville, Ohio) Construction of guar cDNA libraries Seeds ... (Qiagen, Los Angeles, CA) cDNA was prepared from polyA+ enriched, pooled samples of equivalent amounts of total RNA from each time point Two cDNA libraries were gen- Page of 12 (page number not...

Ngày tải lên: 12/08/2014, 05:20

... 600 bp (b) Distribution of sequence length of assembled contigs The average length of contigs was 597 bp (c) Distribution of the number of ESTs per assembled contig Most of the contigs had one ... blastema Number of contigs (+ singlets) The number of expressed sequence tags sequenced from the two libraries blastema and neural tube, as well as the number of contigs, the number of clones in ... Distribution of sequence length Distribution of sequence length (a) Distribution of read lengths of the sequenced ESTs after quality control The average read length was 569 bp, corresponding to a peak of...

Ngày tải lên: 14/08/2014, 14:21

... 232 ESTs from S mansoni cDNA Libraries Methodology 2.1 Construction of cDNA libraries and sequencing The following seven cDNA libraries were used in this study: four libraries (Adult 1-4) from ... al 233 Table Information about the sequencing of different S mansoni cDNA libraries S mansoni cDNA libraries Number Number Number Number of of of of ESTs sequenced clones usable ESTsa> usable ... Quality control of the cDNA libraries state that the sequencing of 100-200 clones from a liSince the start of S mansoni genome project, one of brary is sufficient to assess the quality of this library...

Ngày tải lên: 11/11/2016, 15:59

... chamber of the injection pump with the pressure chamber, is a type of solenoid valve that turns ON/OFF (opens and closes) in accordance with the signals from the engine ECU When the coil of the ... Nozzle Plunger 3-1 Types of Solenoid Spill Valves The solenoid spill valve is directly related to the control of the fuel injection volume Therefore, it must provide high levels of precision, quick ... ending the pumping of fuel Solenoid Spill Valve Coil Pump Chamber Valve Pressure Chamber Coil energized valve closed (during suction or injection) Coil non-energized valve open (end of injection)...

Ngày tải lên: 23/10/2012, 09:09

... biofilm Amounts of added inhibitors were DCMU (an inhibitor of photosynthesis of phytoplankton) mg, ATU (an inhibitor of respiration of nitrifying bacteria) mg, and nystatin (an inhibitor of ... Observation of aquatic biota Investigation of the biota was performed at each point of the both channels in October 2000 Preprocessing of each sample was performed by extracting a 20 cm section of bricks ... and VSS of the suspended sample were also measured Measurement of oxygen production/uptake rate of biofilm An appropriate amount (that will make the VSS after measurement 35 ± 10 mg) of the suspended...

Ngày tải lên: 05/09/2013, 08:40

... detailed discussion of the rise of the concept of fetal rights and fetal protectionism can be found in Daniels, 1993.) After a brief discussion of the social construction of maternity and paternity, ... diVerence generated by the politics of fetal risks, and the problematic nature of the idea of individual causality in discussions of fetal harm Social constructions of maternity and paternity In Western ... circle of causality, essential to a deconstruction of the symbol of the pregnant addict, has proved more diYcult Both metaphorically and literally, fathers were absent from virtually all of the...

Ngày tải lên: 01/11/2013, 08:20

... Generally, these linear references are the edges of the planar surface on the model, any two planar surfaces or axes, or a combination of any of these Figure 5-5 shows the linear hole on a plane ... Surf Chain option allows you to select the chain of edges of the selected surface When you select a surface on the model, the CHAIN OPT submenu is displayed as shown in Figure 5-17 This menu has ... value assigned For example, the depth of extrusion in case of extruded features, the angle of revolution in case of revolved features, and the radius in case of rounds The child features, if any,...

Ngày tải lên: 03/01/2014, 23:56

... placed on the same placement surface and no feature intersects the edges of the placement surface, any other instance, or any other feature other than the placement surface Note that it not possible ... flap of the model shown in Figure 6-5 Varying Pattern The Varying type of pattern is used when the instances vary in size In this type of pattern the instances can be placed on different surfaces ... be extruded to a depth of c The second feature is also an extruded feature, see Figure 6-25 The sketch of the second feature will be created on the right planar surface of the base feature, see...

Ngày tải lên: 03/01/2014, 23:57

... where an interaction of the tidal regimes of the South Chinese Sea and of the Gulf of Thailand eliminate each other Here, the tidal amplitude is less than 10 cm Because of its lower elevation ... water TCVN 5944 –1995 (see table 3) The results of the analyses of the surface water samples were compared to the Vietnamese standards for the use of surface water for drinking water purposes TCVN ... usage of surface water should be minimized despite of its suitability for drinking water purposes Here, it can be expected that the surface water quality is going to be worsened because of its...

Ngày tải lên: 16/01/2014, 17:20

... hydrophobic surface to solvent The subunit interface of yeast enolase is small by the criteria of Tsai et al with only 13% of the surface buried [4] In addition, there are a large number of polar ... Ó FEBS 2004 Native monomers of yeast enolase produced by pressure (Eur J Biochem 271) 3899 Fig UV spectra of yeast enolase at high and low pressures The spectrum of yeast enolase, 10.6 mM, in ... losses of activity due to absorption of enolase to the sides of the cuvette during the 45 incubation under pressure Results Exposure of yeast enolase to hydrostatic pressure in the range of 0.1–240...

Ngày tải lên: 19/02/2014, 16:20

... itemization, there are two sentences in each of the (1) kono software-no riten-ha hayaku ugoku run this software-POST advantage-POST quickly The advantage of this software is to run quickly (2) ketten-ha ... some works that discuss learning the polarity of words instead of sentences Hatzivassiloglou and McKeown proposed a method of learning the polarity of adjectives from corpus (Hatzivassiloglou and ... of product revews In Proceedings of the WWW, pages 519–528 Andrea Esuli and Fabrizio Sebastiani 2005 Determining the semantic orientation of terms throush gloss classification In Proceedings of...

Ngày tải lên: 20/02/2014, 12:20

... purification of the cytosolic PtdEtn-PLD resulted in rapid loss of activity Ó FEBS 2002 Characterization and regulation of yeast PtdEtn-PLD activity (Eur J Biochem 269) 3823 Table Effect of different ... effects of different chloride salts of divalent cations on the membrane-bound and cytosolic PtdEtn-PLD activities assayed in the absence of added EDTA and EGTA, i.e in the presence of  10)5 M of ... and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ions...

Ngày tải lên: 22/02/2014, 07:20

... aggregates of Sup35 [13] The propagation of [PSI+] is strictly dependent on the presence of an appropriate amount of Hsp104 [19] Impairment of Hsp104 function, by either the deletion of Hsp104 ... cytoplasm of [PSI+] cells, regardless of the presence of foci [36] The combination of FCS with the on-chip single cultivation system (a time-lapse FCS system) allows measurements of the size of Sup35–GFP ... induction of [PSI+] by the overexpression of Sup35 in [psi)][PIN+] cells, where [PIN+] is a yeast prion phenotype that requires the induction of [PSI+] [33] Based on their detailed examination of the...

Ngày tải lên: 06/03/2014, 09:22

... distribution of household savings The last set of satellite tables refers to a different way of recording some of the transactions in a SAM A familiar example concerns the allocation of part of public ... Possibilities to so are of course enhanced by the use of computers The rapid development of both hard- and software in the last decade has undoubtedly influenced both the size and accuracy of SAMs The stages ... least, estimates of the size of each household group are needed for the computation of per capita incomes and expenditures Likewise, a decomposition of wages into estimates of employment and...

Ngày tải lên: 06/03/2014, 21:20

... subunits of the type 2A phosphatases PP2Aca (one cDNA) , PP2Acb (three cDNAs), the C-terminal region of PP2Aca ⁄ b (one cDNA) , PP4c (three cDNAs) and PP6c (two cDNAs) Initial mapping of the region of ... as a regulator of the TOR pathway in yeast cells [19], the role of a4 in the mTOR-dependent control of cell growth is still unclear The yeast Tip41 protein was identified in a yeast two-hybrid ... regulator of the rapamycin-sensitive signaling pathway by competing with Sit4 for Tap42 [20] The fission yeast homolog of Tip41 has been characterized as a regulator of the activity of type 2A...

Ngày tải lên: 07/03/2014, 05:20