construction and propagation of first generation viruses

... with 100 μl of DMEM medium free of serum and antibiotic These two solutions were mixed and incubated for 45 at room temperature and then added into another 800 μl DMEM A total of ml of the transfection ... for the advices and suggestions given by Dr Cui Z Z (Animal Science and Technology College, Shandong Agricultural University, China) and for the critical review and suggestions of Dr Xingquan ... promoter and schematic presentation of the bi-directional different The the The schematic presentation of the bi-directional promoter and the parts and directions of the promoter in different constructs...

Ngày tải lên: 12/08/2014, 04:21

... software without detail understanding Despite of the various standard and code, there is limited procedure and rules in designing the floating roof which result lots of floating roof failure and ... Project Limitations of Use The Council of the University of Southern Queensland, its Faculty of Engineering and Surveying, and the staff of the University of Southern Queensland, not accept any ... of the tank shell • Roof seals giving a gap as the result of out -of- roundness and/ or tilting of the roof • Loss of buoyancy of floating roofs due to liquid in pontoon • Roof drain leaking or being...

Ngày tải lên: 15/03/2014, 05:20

... into bands, the first band (about 2.7kb) is the linear backbone of vector pBT, and the another band (about 1.6kb) is 4CL1 gene In line 2, vector pPTN289 was also digested into bands, the first ... 4CL1 using PCR method (line and 2) and NcoI/XbaI double digestion (line and 4) 3.3 Transformation of vector pPTN289-4CL1 into Chinaberrytree and tested the existence of 4CL1 gene using PCR method ... Rifamycin 100mg/l and Spectinomycin 50mg/l) and extracted plasmid, tested using PCR method and NcoI/XbaI double digestion N.V Thanh et al / VNU Journal of Science, Natural Sciences and Technology...

Ngày tải lên: 22/03/2014, 09:20

... DESIGN AND DEVELOPMENT OF MEDICAL ELECTRONIC INSTRUMENTATION DESIGN AND DEVELOPMENT OF MEDICAL ELECTRONIC INSTRUMENTATION A Practical Perspective of the Design, Construction, and Test of Medical ... responsibility for the use of these circuits and/ or software or of systems incorporating these circuits and/ or software lies with the reader, who must apply for any and all approvals and certifications ... provide an assortment of desired passband and gain characteristics selection and preparation of electrode placement on the subject, and by keeping the bandpass characteristics of the biopotential...

Ngày tải lên: 29/03/2014, 11:20

Ngày tải lên: 09/05/2014, 17:04

... (lanes and 2, respectively); DNA amplification of plasmid T3 and the recombinant one (lanes and 4, respectively); RT-PCR on RNA of YF17D/E200T3 and YF17D/Esa/5.1glic 2P viruses without and with ... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... recombinant viruses expressing protozoan and other viral antigens of interest have been developed One of the hallmarks of YF 17D vaccine is its extremely low incidence of adverse events All YF 17D viruses...

Ngày tải lên: 18/06/2014, 18:20

... development and testing of candidate live attenuated vaccines and antiviral therapeutics against CVA16 Methods Cells and viruses RD and Vero cells were grown in DMEM (Gibco, Grand Island, NY, USA) ... 4392) and CV(4381–7410), and subsequently rejoin them via an XbaI site, to yield CV(1–7410) The first strategy is successful for the construction of infectious clones of a number of enteroviruses ... or processing of both viruses Figure Western blot analysis of capsid composition of the recovered viruses Lysates from cells infected with the R1 or R2 generation of recovered viruses or wild-type...

Ngày tải lên: 18/06/2014, 18:20

... domains of the short-form of BPH-QLS and SF-36 were significantly correlated (p < 0.01) with CCs of 0.267~0.773, most of which were > 0.300 The disease domain of the new scale and that of the ... 0.424 for disease and physical; 0.515 for physical and social; 0.777 for social and psychological; and 0.612 for psychological and satisfaction Criterion validity of the short-form of BPH-QLS is ... Health and Quality of Life Outcomes 2009, 7:24 development of shorter questionnaires The aim of this study was to construct and validate the short-form of BPHQLS and to compare its...

Ngày tải lên: 18/06/2014, 19:20

... (lanes and 2, respectively); DNA amplification of plasmid T3 and the recombinant one (lanes and 4, respectively); RT-PCR on RNA of YF17D/E200T3 and YF17D/Esa/5.1glic 2P viruses without and with ... were scraped off and lysed under nondenaturing conditions in the presence of the same protease inhibitors The volume of 250 µL of cell extracts and mL of the cell culture supernatant of each sample ... recombinant viruses expressing protozoan and other viral antigens of interest have been developed One of the hallmarks of YF 17D vaccine is its extremely low incidence of adverse events All YF 17D viruses...

Ngày tải lên: 20/06/2014, 01:20

... development and testing of candidate live attenuated vaccines and antiviral therapeutics against CVA16 Methods Cells and viruses RD and Vero cells were grown in DMEM (Gibco, Grand Island, NY, USA) ... 4392) and CV(4381–7410), and subsequently rejoin them via an XbaI site, to yield CV(1–7410) The first strategy is successful for the construction of infectious clones of a number of enteroviruses ... or processing of both viruses Figure Western blot analysis of capsid composition of the recovered viruses Lysates from cells infected with the R1 or R2 generation of recovered viruses or wild-type...

Ngày tải lên: 20/06/2014, 01:20

... using the cutoff of more than 1000 copies/ml reported a cumulative probability of failure by three years after ART initiation of 19.3% [13], and a study of children and adolescents in Uganda reported ... Bangkok, Thailand School of Public Health and Family Medicine, University of Cape Town, Cape Town, South Africa 3Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland Authors’ ... Graber and Olivia Keiser, Institute of Social and Preventive Medicine, University of Bern, Bern, Switzerland; Andrew Boulle, Morna Cornell, Mary-Ann Davies, Nicola Maxwell, Landon Myer and Anna...

Ngày tải lên: 20/06/2014, 08:20

... access and use in Asia and southern Africa J Int AIDS Soc 2011, 14(1):7 doi:10.1186/1758-2652-14-17 Cite this article as: and : A biregional survey and review of first- line treatment failure and ... 2011, 14:17 http://www.jiasociety.org/content/14/1/17 Page of Switzerland and the School of Public Health and Family Medicine, University of Cape Town, Cape Town, South Africa Received: 28 March ... and Journal of the International AIDS Society 2011, 14:17 http://www.jiasociety.org/content/14/1/17 Page of Table First- and second-line antiretroviral therapy regimens in use in TREAT Asia and...

Ngày tải lên: 20/06/2014, 08:20

... Length of a first- order branch Foliar biomass of a first- order branch Woody biomass of a first- order branch Total (foliage and woody) biomass of a first- order branch Height of the base of a first- order ... three-dimensional coordinates of the base of the main stem of an individual H: height of the leader shoot tip (tree height), z : height of the base of a branch the growth and mortality of first- order branches ... to the size and height of branches, the competitive status of individuals, and the size and growth of individuals MATERIALS AND METHODS 2.1 Study site and data collection At the end of the growing...

Ngày tải lên: 08/08/2014, 14:22

... implementation of Marker Assisted Selection (MAS) programs and the generation of transgenic and knockout cattle will benet from greater characterization of the bovine genome Identication of DNA variations ... shaking, three copies of each 96-well microtitre plate were prepared and stored at 80 C at different locations Superpools of 24 plates and pools of individual plates (24), 12 columns and rows were prepared ... screened for the 31 marker genes of the Texas standard Construction of a bovine BAC library 547 nomenclature of the bovine karyotype [4], providing an accurate set of BAC clones which were used...

Ngày tải lên: 09/08/2014, 18:21

... comprised μl of 5×buffer, μl of 10 mM dNTP mix, μl of each forward and reverse primer (10 pmol/μl), μl of 40 U/μl RNAguard (Invitrogen, Carlsbad, CA, USA), μl of enzyme, and μl of template The ... length of the NA stalk and that stalk deletion has resulted in adaptation of the virus to land-based poultry [26,31] No deletions were found in the stalk regions of the neuraminidase of the viruses ... activation of protein kinase R and the activation of transcription factors NF-κB and IRF-3 [53-57] On the other hand, others claim the primary function of NS1 RNA binding to be inhibition of the...

Ngày tải lên: 12/08/2014, 04:20

... The Netherlands) according to the manufacturer's instruction, and eluted in 55 μl Detection of influenza A virus was performed using primers and probe targeting part of the 5'-end of the Matrix ... Veterinaria Scandinavica 2010, 52:28 http://www.actavetscand.com/content/52/1/28 Page of Table 1: Overview of wild waterfowl sampled for influenza A virus examination in Norway 2006 and 2007 No of birds ... wild waterfowl in Norway 2006-2007 2006 Gulls No of positive/ No of tested 2007 Ducks No of positive/ No of tested 2007 Gulls No of positive/ No of tested 2/102 1/47 14/186 7/123 1.9% 2.1% 7.5%...

Ngày tải lên: 12/08/2014, 18:22

... between the standardized descriptors y (y being one of [Casp3*]max and τ in standardized form) and the standardized random parameters X, where each row of X is a concatenation of the 54 model ... Figure Steady-state profiles of DISC, tBid-Bax2, and apoptosome Steady-state concentrations of DISC, tBidBax2, and apoptosome, used for modularization of the DISC, MAC, and apoptosome modules, ... SG, KLH and KCT equally contributed in constructing and simplifying the model HAH and SG conducted analysis and KLH and KCT performed simulations HAH and KLH prepared the initial drafts of the...

Ngày tải lên: 13/08/2014, 16:21

... assayed by serial culture and restriction enzyme digestion NotI and HindIII restriction digest patterns of 14 randomly chosen BAC clones were compared before and after 100 generations in serial growth ... 10−2 and µL was used to inoculate a new overnight culture This was repeated on day 2, 3, 4, and until 100 generations had passed BAC DNA from each clone was prepared as above on day and NotI and ... Laboratory Two hundred nanograms of DNA was used and the cycling conditions were as follow: 95 ◦ C, and 99 cycles of 95 ◦ C, 30 s; 50 ◦ C, 20 s; 60 ◦ C for RESULTS AND DISCUSSION A channel catfish...

Ngày tải lên: 14/08/2014, 13:22

... Theoretical and practical development of competitive wholesale electricity market Chapter Current status of Vietnam’s electricity market and the necessity of construction and development of Vietnam ... characteristics and conditions of conversion, development of a market typical number of countries around the world each stage of development of the electricity market: competitive generation electricity ... characteristics and principles of operation, conditions for formation and development of the electricity market, the form of organization of the electricity market, going into the analysis of the characteristics...

Ngày tải lên: 15/08/2014, 23:53

... space part of a building from which air and smoke are extracted for the purposes of depressurization 3.9 depth (of a building) distance to the surface of the lowest point of the floor of the lowest ... Fire Brigades Union Health and Safety Executive Home Office Institute of Building Control Institution of Gas Engineers Institution of Structural Engineers London Fire and Civil Defence Authority ... National Association of Fire Officers National Council of Building Material Producers Royal Institute of British Architects Scottish Office (Building Directorate) Timber Research and Development Association...

Ngày tải lên: 28/09/2014, 23:27