constitutive expression of its agt1 gene paper ii iv

... required for maintenance of high PRDX5 gene expression under conditions of oxidative stress We previously found that expression of the PRDX5 gene is strongly inhibited by treatment of human or rat cells ... inducible but also for constitutive expression of some genes [23] About 20-fold silencing of NRF2 expression was reproducibly detected 72 h after NRF2 siRNA transfection of A549 cells (Fig 3A), ... other antioxidant response genes [21] As NRF2 sites in the PRDX5 promoter may be involved in the maintenance of constitutive expression of PRDX5 in the absence of oxidative stress, we examined whether...

Ngày tải lên: 30/03/2014, 11:20

... data on homologous expression of the nrdF gene of C ammoniagenes strain ATCC 6872 This is the first report of the successful purification of high amounts of the native C ammoniagenes R2F as a manganese- ... enzyme of C ammoniagenes in its native species Here, the source of the native R2F-protein of the C ammoniagenes ribonucleotide reductase were transformants from the reintroduction of the nrdF gene ... analysis of its metal composition and similarity of its absorption spectrum to di-manganese(III) model complexes [5] This Mn-RNR was considered as a prototype of an enzyme category of its own...

Ngày tải lên: 15/02/2014, 01:20

... Isolation of strains with altered pyrG expression Strains with different constitutive expression levels of the pyrG gene encoding CTP synthase were isolated using a PCR strategy where the pyrG gene ... stippled lines Fig Eects of changing pyrG expression on the concentration of dCTP The concentration of dCTP is shown as a function of relative b-galactosidase activity in percent of the wild-type level ... modulated pyrG expression at 15 C and 30 C The relative pyrG expression is given as per cent of wild-type b-galactosidase activity The activity in the pyrG strain CJ340 is dened as 0%; activity in...

Ngày tải lên: 16/03/2014, 16:20

... indicated that mutation of the Sp1-binding site decreased the promoter activity by 46.9%, whereas overexpression of Sp1 increased the promoter activity by 58.3% The expression of endogenous KCTD10 ... Alignment of the 5¢-flanking sequences upstream of the start codon of human and mouse KCTD10 B Fig In vivo ChIP assays of Sp1.1 and AP-2.2 binding sites in the proximal promoter region of human ... SP1 and AP-2a regulate KCTD10 Roles of Sp1 and AP-2a in the regulation of the promoter activity and the expression of human KCTD10 Fig Transcription start site of human KCTD10 Antisense primer from...

Ngày tải lên: 30/03/2014, 02:20

... defective in PKA activity and cotransfection of PKAc restores its PKA activity Finally we tested the effect of H-89, a known inhibitor of PKA, on the expression of both the proglucagon gene promoter ... would stimulate endogenous Cdx-2 mRNA 2747 Activation of Cdx-2 expression by cAMP A ii) GLUTag i) GLUTag Relative LUC Activity L Chen et al iii) HT-29 iv) GLUTag 1.2 ** 0.8 * v) GLUTag ** ** 1 Reporter ... 2746–2759 ª 2005 FEBS L Chen et al Activation of Cdx-2 expression by cAMP Fig Comparison of the effect of H-89 on expression of the proglucagon and Cdx-2 gene promoters Three micrograms )476 GLU-LUC...

Ngày tải lên: 30/03/2014, 16:20

... activation of NFκ-B [23] and IRF-3 [24] which are necessary for IFN-β synthesis in virus infected cells Influenza B virus NS1 protein also inhibits activation of IRF-3 [19], and the activity of ... Aspehaug VT: Characterization of major structural proteins of the infectious salmon anaemia virus (ISAV) In Doctoral Thesis University of Bergen, Norway; 2005 Paper III Clouthier SC, Rector T, Brown ... giving the virus an advantage in its fight against the antiviral host response to infection The complete ISAV protein profile has not yet been definitively established ISAV segment 8, like its...

Ngày tải lên: 19/06/2014, 08:20

... activation of NFκ-B [23] and IRF-3 [24] which are necessary for IFN-β synthesis in virus infected cells Influenza B virus NS1 protein also inhibits activation of IRF-3 [19], and the activity of ... Aspehaug VT: Characterization of major structural proteins of the infectious salmon anaemia virus (ISAV) In Doctoral Thesis University of Bergen, Norway; 2005 Paper III Clouthier SC, Rector T, Brown ... giving the virus an advantage in its fight against the antiviral host response to infection The complete ISAV protein profile has not yet been definitively established ISAV segment 8, like its...

Ngày tải lên: 20/06/2014, 04:20

... optical density (IOD) of mPER1 immunoreactivity, an index of mPER1 protein expression level, was analyzed by image pro plus software Time point means and SE of protein expression are shown along ... observed High expression of mPER1 is seen as brown-yellow, whereas low expression is seen as blue in the sections (Figure 3) Rhythmic expression of mPER1 was analyzed according to the mPER1 expression ... schedules could entrain the circadian rhythm of clock gene expression in the liver independent of the SCN [29,30] These findings suggest that the circadian rhythms of peripheral organs may be synchronized...

Ngày tải lên: 10/08/2014, 09:20

... to a square of cm2 in our experimental setup In experiment 1, we investigated the sensitivity and reproducibility of our device and software to detect the exact frequency of movement of a small ... United Kingdom) and a white square paper of variable surface This paper was placed perpendicularly in front of the camera We recorded a series of movements of the mobile at different speeds and ... http://www.jcircadianrhythms.com/content/4/1/10 The recordings of days of activity of different rats in the second experiment were similar to those presented in Figure 5B, top Actograms are plots of the number of pixels changed (y-axis)...

Ngày tải lên: 10/08/2014, 09:20

... numbers of individuals tested (60.7% of HAM/TSP patients, 34 out of 56; 71.1% of HCs, 27 out of 38; 30.0% of ATL patients, out of 10), whereas HTLV-1 HBZ mRNA was expressed in all the infected individuals ... HCs – ATL) The zero value of tax gene transcripts was observed in 60.7% of HAM/TSP patients (34 out of 56), 71.1% of HCs (27 out of 38) and 30.0% of ATL patients (3 out of 10) The medians are represented ... staff and blood donors of Kagoshima University Hospital We also thank Dr Ryuji Kubota for providing the clinical samples, Prof Masahiro Fujii of Niigata University for the gift of HTLV-1-infected...

Ngày tải lên: 12/08/2014, 23:20

... influenced (positively, or negatively) by the level of core expression as determined by gene dose (i), and gene expression efficacy (ii) The first issue was addressed in a series of immunizations ... recognition sites of HindIII and XhoI restriction endonucleases The amplified DNA was cleaved with HindIII/XhoI and inserted into pcDNA3 (Invitrogen, USA) cleaved with HindIII/XhoI resulting ... response is to deliver limited intracellular amounts of core, either by giving lower plasmid doses, or by giving vectors with low expression efficacy An additional option is the use of heterologous...

Ngày tải lên: 14/08/2014, 19:22

... transactivates the reporter gene expression (B) Two-hybrid gene switch: the chimeric genes, DBD:EcR LBD (C) and AD:RXR LBD (D) are under the control of constitutive promoters The heterodimer of these ... almost same as that of the background luciferase activity observed with CH9 and CfEcRWt (Fig 3A) The relative light units (RLU) per microgram of protein of luciferase reporter gene expression differed ... demonstration of its utility in regulating the expression of transcription factor in plants The ability to tightly regulate gene expression in plants is an essential tool for the elucidation of gene function...

Ngày tải lên: 16/03/2014, 06:20

... Evaluation of its presence in PSIIRC was allowed by the treatment of the wild-type preparation with SDS in the ratio SDS/ chlorophyll ˆ 10 Deriphat PAGE of this preparation led to the generation of PSIIRC ... by native Deriphat/PAGE of PSII complexes isolated from wild-type and IC7: A, PSII core ˆ PSII complex containing at least CP47, CP43, D1, D2 and cytochrome b-559; B, PSII subcore ˆ PSII core ... important factor regulating process of PSII repair Its removal from PSII could result in a complete disassembly of PSII during the D1 replacement while in its presence the D1 replacement could...

Ngày tải lên: 17/03/2014, 11:20

... CA: Inhibition of BCL11B expression leads to apoptosis of malignant but not normal mature T cells Oncogene 2007, 26:3797-3810 Livak KJ, Schmittgen TD: Analysis of relative gene expression data ... for The relative amounts of the genes of interest and the b2M reference gene were measured in two independent assays The 2(-ΔΔCT) method was used to present the data of the genes of interest Huang ... 2Department of Hematology, Guangdong General Hospital (Guangdong Academy of Medical Sciences), Guangzhou, 510080, PR China Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University,...

Ngày tải lên: 10/08/2014, 22:21

... of GLP-1 (7-37) peptide A sequence of nucleotide including 45 bases was used to connect the 3’ terminus of GLP-1-A gene and 5’ terminus of gAd gene The product of this nucleotide sequence was a ... However, the clinical application of native GLP-1 is hampered by its very short plasma half-life [5, 6, 13] This is due to its rapid inactivation by dipeptidyl peptidase IV and by renal clearance [5, ... terminus to form bioactive peptides [4] The principal biologically active forms of GLP-1 are: GLP-1 (7-37) and the predominant circulating active form GLP-1 (7-36) amide [4] In vivo, both peptides...

Ngày tải lên: 25/10/2012, 11:10

... [7] The number of mature liver schizonts increased in the presence of mAbs anti-CSP of P yoelii and the authors suggested that enhancement of infection could be a consequence of an interaction ... histocompatibility complex class II expression by disruption of the Jak/Stat pathway J Exp Med 1998; 187: 675-683 21 Lidbury BA, Mahalingam S Specific ablation of antiviral gene expression in macrophages ... relative number of DNA molecules Triplicates of each sample and standard curve were performed in all assays The quantity of cDNA for csp was normalised to the quantity of the house keeping gene...

Ngày tải lên: 02/11/2012, 10:14

... targeted disruption of the Dio2 gene have cold-induced overexpression of the uncoupling protein gene but fail to increase brown adipose tissue lipogenesis and adaptive thermogenesis Diabetes 2004, ... proton motive force as heat instead of ATP synthesis [23,24] As shown in Figure 6, consistent with the fact that PGC-1a induces mitochondrial biogenesis and thermogenesis [25], its gene expression ... 36.6 We next determined gene expression in different adipose depots in response to PM2.5 exposure, in terms of the expression of BAT-specific and WAT-specific gene profiles by real-time PCR analysis...

Ngày tải lên: 06/03/2014, 19:20

... profiles is given in arbitrary units (AU), in which case one unit of activity refers to an increase of 0.001 absorbance units per microlitre of sample and per min, but normalized for the volume of sample ... recommended use of butyrylcholinesterase overexpression as a predictive survival index in liver cancer [51] Considering the butyrylcholinesterase contribution to immortalization of several SV40-transformed ... according to the Heidelberg Classification of Renal Cell Tumours [2] Seven specimens of pRCC, seven of cRCC, six of chRCC, and six of RO, besides adjacent pieces of unaffected tissue, were used in this...

Ngày tải lên: 06/03/2014, 22:21

... ability of the AR to activate the transcription of AR-regulated genes [77] Because Fli-I does not enhance AR activity by itself, but requires the presence of a p160 coactivator, binding of Fli-I ... b-actin is located at the promoter sequences of an actively transcribed U6 gene in vivo, which suggests that it participates in the transcription of Pol III [27,29,30] Upon treatment with methane ... with the dissociation of Pol III, which suggests that b-actin dissociates from the Pol III complex Third, many experiments have shown that b-actin is required for Pol III transcription [27,29,32]...

Ngày tải lên: 07/03/2014, 01:20

... coactivators that regulate the expression of genes playing key roles in the biogenesis of the OXPHOS system In general, the transcription of nuclear genes encoding proteins involved in OXPHOS biogenesis ... correct expression of several homeotic genes It was first identified as an activator of the engrailed and Ultrabithorax promoters, and was later shown to regulate the activity of several constitutive, ... transcript The expression of the a-F1-ATPase gene during development is coordinated with the expression of the nuclearencoded b-F1-ATPase gene and the mitochondrial-encoded ATPase and genes [27]...

Ngày tải lên: 07/03/2014, 16:20