consequences of protein n homocysteinylation by hcy thiolactone

... heterogenous network by merging the three types of subnetworks (protein- protein interaction network, lncRNA-lncRNA co-expression network, and protein- lncRNA association network) The resulting network ... heterogeneous network Lei Deng1 , Junqiang Wang1 , Yun Xiao1 , Zixiang Wang1 and Hui Liu2* Abstract Background: Identifying the interactions between proteins and long non-coding RNAs (lncRNAs) is of ... consists of five steps (A) Collection of three types of data sources, including protein- protein interaction network, proteinlncRNA associations and lncRNA co-expression network (B) Construction

Ngày tải lên: 25/11/2020, 14:38

... did not cause a loss of function [21]. In animal cells, one type of Ser ⁄ Thr protein kinase, protein kinase C (PKC), is involved in signal transduc- tion pathways that govern a wide range of ... may depend on a decline in the number of active channels or on a lower single-channel conductance, as the voltage-dependent characteristics were not affected by PMA application in oocytes injected ... cells may also have an equivalent in plant cells. Ca 2+ plays an important role as an intracellular signal in both plants and animals, including its involvement in the regulation of CDPK activity

Ngày tải lên: 22/03/2014, 21:21

... Daolong Ren1,*, Ailan He1, Xiang Hu1, Wenfeng Zhang1, Liping Yang1, Mingjun Liu1, Hong Li1, Jianlin Zhou1, Shuanglin Xiang1 and Jian Zhang1,2 Key Laboratory of Protein Biochemistry and Development ... Technology of China (No 2005CB522505, 2006CB943506), the Hunan Provincial Natural Science Foundation of China (No 08JJ3082), Project of Hunan Science and Technology Commission: run foundation of ... interference, in which overexpression of one transcription factor resulted in inhibition of another, was often found in the regulation of eukaryotic gene expression The repression of AP-2 on Sp1dependent

Ngày tải lên: 30/03/2014, 02:20

... domains of unknown function. The residue numbering indicative of the beginning and end of each domain is shown on the right. D-Serine synthesis is measured under various conditions in the absence ... Ca 2+ concentrations, a clear indication that GRIP binding does not result in a higher sensitivity of SR towards this divalent cation. ATP binding does not involve significant changes either in the ... glutamate, ATP and d-serine. Released glutamate and d-serine occupy binding sites in the NMDA receptors of neurones hence regulating neuronal function. In this context, we analysed how brain SR becomes

Ngày tải lên: 30/03/2014, 03:20

... endothelial NOS relative to neuronal NOS The interaction of nitric oxide synthase (NOS) with a variety of proteins plays an important role in the regulation of NO production [1–4] Of these interacting proteins, ... shown that, in vivo, under conditions of calpain activation, the protection of NOS degradation occurs In addition, the extent of NOS degradation is a function of the level of HSP90 expression Thus, ... form, can be reproduced in in vitro conditions if nNOS digestion by calpain is carried out in the presence of HSP90 [27] This finding can also explain the large extent of digestion of eNOS in aorta,

Ngày tải lên: 30/03/2014, 04:20

... Determination of modulation of ligand properties of synthetic complex-type biantennary N- glycans by introduction of bisecting GlcNAc in silico , in vitro and in vivo Sabine Andre ´ 1 , Carlo Unverzagt 2, *, ... and in vivo procedures. The question of whether introduction of the bisecting GlcNAc changes the ligand properties (binding affinities) of biantennary N- glycans for branch-end-specific lectins as ... dampening effect on the extent of conformational fluctuations and dynamics of intramolecular mobility. The way in which the bisecting substituent shapes the population density of the biantennary nonasaccharide

Ngày tải lên: 30/03/2014, 13:20

... shifts of all assigned protons in D17|29 zinc rubredoxin were measured in the presence of different solute concentrations. Variation of NH amide chemical shifts along the protein backbone dem- onstrated ... We found that KCl had no significant effect on the half-life of the proteins examined (Table 1). Structure determination of mutant D17|29 by NMR Proton signal assignment was performed using the ... Therefore, the tendency to increase the coefficients in the presence of the stabil- izing solutes reflects the strengthening of the hydrogen bond network. These findings are in line with an ear- lier

Ngày tải lên: 30/03/2014, 15:20

... subunits of PP1 Searching completed genome sequences, with the sequences of known PP1 regulatory subunits, including the conserved PP1 and glycogen-binding regions of the glycogen-targeting subunits, ... genomic sequence and partial cDNAs in the database (B) Human PPP1R3E cDNA and the encoded protein determined by PCR of human brain and testis cDNA libraries The PP1 binding motif is underlined, ... magnetic-resonance spectroscopy New Eng J Med 322, 223–228 Cohen P (1999) The Croonian Lecture 1998 Identification of a protein kinase cascade of major importance in insulin signal transduction Phil Trans

Ngày tải lên: 30/03/2014, 16:20

... Stern–Volmer constant for collisional quenching and v is the static quenching constant. Iodide quenching did not show a significant static component, and then the exponential term was not included ... obtained by fitting of fluores- cence intensity at 335 nm vs. concentration of quenching agent (similar values were obtained by fitting the intensities at 336, 337 and 338 nm, data not shown). Experiments ... buffer and 150 m M NaCl. Protein concentration was 20–60 l M and absorbance was monitored at 280 nm. No differences in the elution volumes were observed when the protein concentration was increased.

Ngày tải lên: 31/03/2014, 01:20

... response Regulation of apoptosis Regulation of transcription Signal transduction MYA Number of distinct protein domains Number of distinct protein domains (a) LECA Unikonta Opisthokonta Metazoa Chordata ... [23]. Protein domains are minimal structural and evolutionary units in proteins, retai ning their structure and usually their function even when being part of proteins with different domain architec- ... Ed Ed Number of distinct protein domains Figure 3 Dynamics of genomes during animal evolution. The functional contents of inferred ancestral genomes from the LECA to hominids (humans and great

Ngày tải lên: 09/08/2014, 22:23

... contains a putative nuclear localization signal and a potential transmembrane spanning region (indicated in Figure 1), seven potential internal myristoylation sites, one N- glycosylation site and one ... silencing of the endogenous tobacco PKV gene by antisense expression resulted in a taller stature and increased numbers of influorescences in transgenic plants The combined results of reversal of ... producing more GA than the control, non-transformed plants Transcript levels of genes involved in GA biosynthesis and leaf expansion The dwarfing phenotype of XS plants and its reversal by exogenous

Ngày tải lên: 12/08/2014, 03:21

... PsbP protein levels and PSII functionality Conclusion Many of the proteins found to change in accumulation in response to NO3- were directly involved in the assimilation of this mineral nutrient ... could depend on the need of responding to the new nutritional status In leaves, many proteins were found to be (in)directly involved in the photosynthesis reactivation and in the maintenance of the ... accumulation changes induced by nitrate in both roots and leaves of Zea mays plants The attention was focused on the changes in the pattern of protein soluble fractions caused by the addition of 10 mM nitrate

Ngày tải lên: 12/08/2014, 03:21

... be more essential than non-central proteins in modular organization of the protein- protein interaction network Protein validation by Western blot and Immunohistochemistry As shown in Figure 4A, ... degree (7) belonging to the most central protein The most central protein tends to be more essential than non-central proteins in modular organization of the protein- protein interaction network These ... spanned by the assigned peptides divided by the sequence length TNFR1 signaling pathway, and p38 MAPK signaling pathway, etc (Additional file 3) Construction of the protein- protein interaction network

Ngày tải lên: 12/08/2014, 04:20

... using fluorescence Even at ATP concentrations of 15 lm in the phosphorylation reaction mix, resulting in a final concentration below 150 nm in the fluorescence measurements, the interaction between ... containing HSL, creating difference spectra reflecting the interaction between bisANS and HSL The amount of HSL used for each spectrum ranged between 0.8 lg and 2.4 lg, depending on the concentration ... bis-ANS to nonphosphorylated HSL, we measured the fluorescence of bis-ANS in complex with HSL With an excitation wavelength of 296 nm, the emission was scanned between 300 nm and 550 nm at bis-ANS...

Ngày tải lên: 18/02/2014, 11:20

... membrane and the inner membrane channel-forming proteins, TOM40 and translocase of inner membrane 23 (TIM23) [18–20] Our studies defined two distinct mechanisms of activation of cryptic mitochondria-targeting ... mitochondria-targeting signals when fused to the N- terminus of a reporter protein, DHFR [44] This does not mean that such internal signals are functional under physiologic settings while working with an intact protein ... incubated with increasing concentrations of digitonin (0–0.4%; 0–400 lgÆmg)1 protein) for 30 as described in the text The digitonin-insoluble (pellet, left panel) and digitonin-soluble (supernatant,...

Ngày tải lên: 18/02/2014, 16:20

... during intoxication of mammalian cells J Biol Chem 269, 20607–20612 16 Benson EL, Huynh PD, Finkelstein A & Collier RJ (1998) Identification of residues lining the anthrax protective antigen channel ... fragment in VVA2 was identified as the membrane-binding domain [6] These results suggest that the N- terminal and the C-terminal pair of a-helices of VVA1 can bind to VVA2 independently of each ... natural environment and was used for further experiments to determine interactions between VVA2 and VVA1 The input controls for the representative pull-down experiment shown in Fig 4A are lanes...

Ngày tải lên: 19/02/2014, 06:20

... regulation of translation by TRAIL has not previously been investigated We therefore examined the effect of increasing concentrations of TRAIL on the incorporation of [35S]methionine into total protein ... extensive dephosphorylation of 4E-BP1, and this too is a caspase-8-dependent phenomenon Consistent with its effects on the regulation of protein synthesis, IFNa enhances the extent of activation ... means ± SEM of three determinations Insets: percentage inhibition of protein synthesis as a function of TRAIL concentration in cells without IFN (squares) or with prior IFN treatment (triangles)...

Ngày tải lên: 19/02/2014, 06:20

... in buer B, pH 7.8 The inset shows uorescence intensity change in Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of ... Trp85 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used was ... cAMP-binding site at high concentration of the ligand [8] The idea of the four cAMP-binding sites in CRP, for its anti conformation (at low concentration of the ligand) and the next two binding it...

Ngày tải lên: 20/02/2014, 23:20

... absence of added Fe2+EDTA; (n) nonphotolysed/non H2O2 containing samples in presence of added Fe2+EDTA; (r) nonphotolysed/non H2O2 containing samples in absence of added Fe2+EDTA Statistical analyses ... preliminary report has appeared on the inhibition of glutathione reductase by radiation-generated protein peroxides [34], and evidence presented for inhibition of enzymes, by radiation-generated ... inactivation of GAPDH being metalion independent, whereas inhibition of GR and LDH by H2O2 occurs most rapidly in the presence of metal ions It is proposed that the inhibition of GAPDH and GR by these...

Ngày tải lên: 21/02/2014, 03:20

... Site-directed mutational analysis for the ATP binding of DnaA protein Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP-binding domain of DnaA protein in vitro and in vivo J ... comparison of the ATP dependence of EMSA and binding of ATP to purified protein under optimized conditions may not be appropriate Nor can the concentration of ATP necessary to disrupt IRP–IRE binding on ... Constable A, Hirling HK, Kuhn LC & Hentze MW (1993) Recombinant ¨ iron-regulatory factor functions as an iron-responsiveelement-binding protein, a translational repressor and an aconitase A functional...

Ngày tải lên: 07/03/2014, 09:20