consequences of protein n homocysteinylation by hcy thiolactone
Tài liệu Báo cáo khoa học: Stefin A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc
... the inhibition of the enzyme by their endogenous protein inhibitors cystatins and stefins [21] A further step in understanding of these mechanisms was provided by the crystal structures of human ... structure of cathepsin B appears to be rigid A comparison of the interaction constants of the binding of chagasin (Ki = 0.93 nm [25]) and stefins (1.7 and nm [33,34], 0.91 nm [35]) to cathepsin B indicates ... ste n interactions with exopeptidases are not unique The N- terminal trunk of ste n A can displace the A B C D Fig The extent of the occluding loop displacement in the unliganded and liganded...
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Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... using fluorescence Even at ATP concentrations of 15 lm in the phosphorylation reaction mix, resulting in a final concentration below 150 nm in the fluorescence measurements, the interaction between ... containing HSL, creating difference spectra reflecting the interaction between bisANS and HSL The amount of HSL used for each spectrum ranged between 0.8 lg and 2.4 lg, depending on the concentration ... bis-ANS to nonphosphorylated HSL, we measured the fluorescence of bis-ANS in complex with HSL With an excitation wavelength of 296 nm, the emission was scanned between 300 nm and 550 nm at bis-ANS...
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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf
... membrane and the inner membrane channel-forming proteins, TOM40 and translocase of inner membrane 23 (TIM23) [18–20] Our studies defined two distinct mechanisms of activation of cryptic mitochondria-targeting ... mitochondria-targeting signals when fused to the N- terminus of a reporter protein, DHFR [44] This does not mean that such internal signals are functional under physiologic settings while working with an intact protein ... incubated with increasing concentrations of digitonin (0–0.4%; 0–400 lgÆmg)1 protein) for 30 as described in the text The digitonin-insoluble (pellet, left panel) and digitonin-soluble (supernatant,...
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Tài liệu Báo cáo khoa học: Inhibitory effects of nontoxic protein volvatoxin A1 on pore-forming cardiotoxic protein volvatoxin A2 by interaction with amphipathic a-helix doc
... during intoxication of mammalian cells J Biol Chem 269, 20607–20612 16 Benson EL, Huynh PD, Finkelstein A & Collier RJ (1998) Identification of residues lining the anthrax protective antigen channel ... fragment in VVA2 was identified as the membrane-binding domain [6] These results suggest that the N- terminal and the C-terminal pair of a-helices of VVA1 can bind to VVA2 independently of each ... natural environment and was used for further experiments to determine interactions between VVA2 and VVA1 The input controls for the representative pull-down experiment shown in Fig 4A are lanes...
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Tài liệu Báo cáo khoa học: Interferon-a induces sensitization of cells to inhibition of protein synthesis by tumour necrosis factor-related apoptosis-inducing ligand ppt
... regulation of translation by TRAIL has not previously been investigated We therefore examined the effect of increasing concentrations of TRAIL on the incorporation of [35S]methionine into total protein ... extensive dephosphorylation of 4E-BP1, and this too is a caspase-8-dependent phenomenon Consistent with its effects on the regulation of protein synthesis, IFNa enhances the extent of activation ... means ± SEM of three determinations Insets: percentage inhibition of protein synthesis as a function of TRAIL concentration in cells without IFN (squares) or with prior IFN treatment (triangles)...
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Tài liệu Báo cáo khoa học: Steady-state and time-resolved fluorescence studies of conformational changes induced by cyclic AMP and DNA binding to cyclic AMP receptor protein from Escherichia coli ppt
... in buer B, pH 7.8 The inset shows uorescence intensity change in Trp13 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of ... Trp85 as a function of cAMP concentration F and F0 are the uorescence intensities of the protein in the presence and absence of the ligand, respectively The range of cAMP concentrations used was ... cAMP-binding site at high concentration of the ligand [8] The idea of the four cAMP-binding sites in CRP, for its anti conformation (at low concentration of the ligand) and the next two binding it...
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Tài liệu Báo cáo khoa học: Inhibition of glyceraldehyde-3-phosphate dehydrogenase by peptide and protein peroxides generated by singlet oxygen attack docx
... absence of added Fe2+EDTA; (n) nonphotolysed/non H2O2 containing samples in presence of added Fe2+EDTA; (r) nonphotolysed/non H2O2 containing samples in absence of added Fe2+EDTA Statistical analyses ... preliminary report has appeared on the inhibition of glutathione reductase by radiation-generated protein peroxides [34], and evidence presented for inhibition of enzymes, by radiation-generated ... inactivation of GAPDH being metalion independent, whereas inhibition of GR and LDH by H2O2 occurs most rapidly in the presence of metal ions It is proposed that the inhibition of GAPDH and GR by these...
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Tài liệu Báo cáo Y học: Enhancement by a-tocopheryl hemisuccinate of nitric oxide production induced by lypopolysaccharide and interferon-c through the upregulation of protein kinase C in rat vascular smooth muscle cells docx
... LPS/IFN (Fig 2B), as well as NO production Accordingly, we concluded that the enhancing effect of TS on LPS/IFN-induced NO production is caused by enhancement of iNOS protein induction Effects of ... TS-enhanced NO production In addition, a-T reduced the amount of TS-enhanced iNOS protein induced by LPS/IFN (data not shown) It is noteworthy that TA and TN also decreased the enhancing effect of ... TS-enhanced NO production (data not shown) Effects of TS on NO productions induced by single additions of various concentrations of LPS and IFN NO production is reported to be stimulated by LPS...
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Báo cáo khoa học: Inhibition of an iron-responsive element/iron regulatory protein-1 complex by ATP binding and hydrolysis docx
... Site-directed mutational analysis for the ATP binding of DnaA protein Functions of two conserved amino acids (Lys-178 and Asp-235) located in the ATP-binding domain of DnaA protein in vitro and in vivo J ... comparison of the ATP dependence of EMSA and binding of ATP to purified protein under optimized conditions may not be appropriate Nor can the concentration of ATP necessary to disrupt IRP–IRE binding on ... Constable A, Hirling HK, Kuhn LC & Hentze MW (1993) Recombinant ¨ iron-regulatory factor functions as an iron-responsiveelement-binding protein, a translational repressor and an aconitase A functional...
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Báo cáo khoa học: Activation of nematode G protein GOA-1 by the human muscarinic acetylcholine receptor M2 subtype Functional coupling of G-protein-coupled receptor and G protein originated from evolutionarily distant animals doc
... membrane fraction containing 10 lg recombinant protein was added to 100 lL of the phosphate buffer containing 0.1 nm [3H]QNB or nm [3H]NMS in the presence or absence of 0.2 mm atropine, a full antagonist ... CCTATGTTCTTATAATG-3¢ (An engineered EcoRI recognition site is single-underlined The start codon is double-underlined The modified original start codon of M2 is dot-underlined The engineered region containing mycepitope ... point represents the mean ± SEM (A) Binding of [3H]QNB and [3H]NMS to myc-M2 (N- D)I3del::GOA-1 The membrane fraction containing 10 lg recombinant protein was tested (B) Displacement by atropine...
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Báo cáo khoa học: Fluorescence quenching and kinetic studies of conformational changes induced by DNA and cAMP binding to cAMP receptor protein from Escherichia coli ppt
... high-afnity binding sites involves a sequence of protein conformational changes, which shift the protein from a low-afnity nonspecic DNA-binding protein to a state of the protein that binds DNA with ... different DNA sequences leads to long-distance conformational signal transmission from the C-terminal domain to the N- terminal domain of the protein Furthermore, we present the kinetics of DNACRP interactions, ... relative orientation of the subunits and a change in orientation of the DNA-binding domain relative to the cAMPbinding domain [6] Indeed, our Forster resonance ă energy transfer (FRET) measurements show...
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Novel Frontiers of Advanced Neuroimaging Edited by Kostas N. Fountas ppt
... field by a linear combination of low frequency functions based on cubic B-splines adjusted by a cost function based on the intensity and the gradient of the image (Manj n et al., 2007) 2.2.4.2 Non-linear ... processes of the disease: active inflammatory demyelination and neuronal injury in both lesional and non-lesional brain tissue [63, 64] Acute demyelinating lesions reveal increased Cho and Lac resonance ... of Advanced Neuroimaging localized in central and peripheral nervous system and it is synthesized in brain mitochondria Its concentration subtly varies in different parts of the brain [10] and...
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Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... differences between the different B¢¢ subunits are located However, this Xenopus protein has a long N- terminal extension, and so encodes a protein of about 70 kDa instead of 48 kDa Screening of Xenopus ... in Fig 2B, these fusion proteins run at the expected height, proving that no internal methionine was used as initiation start in the translation of the wild type mRNA of XN73 and that the XN73 ... subunit, independently of PR65/A, we developed the same blot as shown in Fig with a monoclonal antibody to PP2AC and found in lane (GST-XN73) no signal that was any higher than found in lane (GST)...
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Báo cáo Y học: High affinity binding between laminin and laminin binding protein of Leishmania is stimulated by zinc and may involve laminin zinc-finger like sequences doc
... laminin–LBP interaction (Fig 3A) The protein denaturant urea at M prevented binding, indicating again that the interaction is conformation-dependent Increasing the NaCl concentration to 0.3 M also significantly ... coated onto nitrocellulose discs and incubated with increasing concentrations of laminin under different conditions (shown on the right of the graph) (B) The influence of different divalent metal ions ... fragments of monoclonal antibodies against various chains of laminin are indicative of the presence of LBP binding site on the B1 chain of laminin Moreover, a number of small peptide recognition...
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Báo cáo khoa học: Bacitracin inhibits the reductive activity of protein disulfide isomerase by disulfide bond formation with free cysteines in the substrate-binding domain pptx
... percentage of the A562 nm of the control reaction containing no inhibitor after subtraction of the A562 nm of the uncatalyzed reaction Data are presented as mean ± standard error of independent ... mechanism of bacitracin binding as shown here for PDI applies to other proteins containing free cysteines In conclusion, we show a mechanism of action of bacitracin on PDI that involves covalent ... percentage of the absorbance of the control reaction containing no inhibitor, after subtraction of the absorbance of the uncatalyzed reaction containing no PDI IC50 values were determined by applying...
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Báo cáo khoa học: Inactivation of tyrosine phenol-lyase by Pictet–Spengler reaction and alleviation by T15A mutation on intertwined N-terminal arm docx
... depletion of the cofactor in the reaction solution, inactivating the enzyme depending on the cofactor binding affinity T15A mutation effect on C freundii TPL cofactor stability indole-lyase (TNA; ... hydrogen bond networks in vicinity of Thr15 on intertwined N- terminal arm H-bond information was extracted from entry code 1TPL of Protein Data Bank In summary, the proximate interaction of Thr15 ... the enzyme by preventing cyclic condensation between l-DOPA and PLP (Fig 1) Results Random mutagenesis and structural identification of T15A mutant An error-prone PCR of C freundii TPL and subsequent...
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![Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx](https://media.store123doc.com/images/document/14/rc/cf/small_cfu1394954409.jpg)
Báo cáo khoa học: Recognition of DNA modified by trans-[PtCl2NH3(4hydroxymethylpyridine)] by tumor suppressor protein p53 and character of DNA adducts of this cytotoxic complex potx
... fraction of noncross-linked DNA in combination with the rb values and the fragment size The DNA interstrand cross-linking efficiency of the new analogue of transplatin was almost independent Fig ... different DNA binding mode correlates with considerably enhanced efficiency of DNA adducts of this new complex to inhibit binding of p53 protein to its consensus DNA recognition sequence In conclusion, ... complex containing the leaving ligands in the trans configuration to inhibit binding of the p53 protein to its consensus DNA sequence Thus, in this respect the new transplatin analogue, trans-PtHMP,...
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Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt
... combination of amino acids at positions and on protein N- myristoylation, sequential vertical-scanning mutagenesis of the amino acids at positions and in a model substrate protein having a sequence ... Effect of the amino acid residue at position in N- myristoylation consensus motif on the efficiency of cotranslational N- myristoylation reaction The mRNAs encoding MG6X-TNF were translated in vitro in ... an amide bond by NMT However, not all proteins with an N- terminal glycine are N- myristoylated and the ability to be recognized by NMT depends on the downstream amino acid sequence In addition,...
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Báo cáo khoa học: Selective modulation of protein C affinity for EPCR and phospholipids by Gla domain mutation pdf
... sequential injections containing decreasing concentrations of protein C (concentration range 18–200 nM) (Lower) Lack of protein C binding sEPCR with substitution E86A The response level of this ... structure–function relationships have been identified by in vitro mutagenesis and expression of recombinant protein C variants Using this approach, loss- and gain -of- function variants have been characterized ... APC–EPCR interaction Selective perturbation of protein C ⁄ APC Gla domain functions therefore represents a potential means by which the anticoagulant and nonanticoagulant functions of APC can be altered...
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Báo cáo khoa học: Microperoxidase 8 catalysed nitrogen oxides formation from oxidation of N-hydroxyguanidines by hydrogen peroxide ppt
... oxidation by H2O2 to a new compound identied as Nx-nitroso-L-arginine without any signicant accumulation of NO or L-citrulline [19] N- aryl -N -hydroxyguanidines, N- aryl ureas and N- aryl cyanamides ... thus obtained by difference between the two measurements Calibration curves were made under identical conditions in the presence of known amounts of NaNO2 and KNO3 Separations of organic products ... mechanisms can generate nitric oxide NO and nitroxyl anion, NO Formation of N2 O could explain the marked differences observed between the amounts of nitrogenous compounds derived from NO (NO2+ NO3)...
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