connecting cell cycle control to cell death regulation in human leukemia

... Dual cell- death checkpoints during TNFR1 signaling M A O’Donnell and A T Ting (TRAFs) and cellular inhibitor of apoptosis protein (cIAPs) was identified as a key checkpoint in TNFR1 death signaling ... for binding K63-linked polyubiquitin chains than head to tail conjugated linear polyubiquitin [21] Because RIP1 is modified with predominantly K63-linked polyubiquitin chains [22], specific binding ... host to possess the capability to disrupt the first cell- death checkpoint in order to trigger apoptosis or necrosis of the infected cells Induction of programmed necrosis in virally infected cells...

Ngày tải lên: 28/03/2014, 23:20

... However, budding yeast Wee1 has recently been shown to delay entry into mitosis and to be required for cell size control, suggesting that mechanisms controlling entry into mitosis in budding yeast ... diploids in the mitotic cell cycle (reviewed in refs and 10) The Mitotic Cell Cycle of Yeasts 3.1 Budding Yeast In budding yeast, a point exists in mid-G1 after which the cell becomes committed to ... of the cell cycle in fungi and flies provided many of the major insights into cell cycle regulation This approach in plants is still in its early days, with cell cycle mutants resulting mainly...

Ngày tải lên: 08/04/2014, 12:50

... (cryopyrin) does not contain a caspase-activating recruiting domain (CARD), the adaptor protein termed apoptosis-associated speck-like protein containing a C-terminal CARD (ASC) is required together ... leads to inflammation but also causes an inflammatory form of cell death termed as ‘pyroptosis’ (Cookson and Brennan 2001) Pyroptosis occurs in response to infection by a number of intracellular ... (H1N1) and compared to wild-type controls To explore the roles of caspase-1 and other mediators of cell death by means of inhibitors in vitro, to complement the in vivo studies Inhibitory studies with...

Ngày tải lên: 13/10/2015, 16:41

... activity using an inhibitor did not lead to the accumulation of APP-CTFs in DRMs APP and APP-CTFs are generally more difficult to detect in human brain material than in rat brain, probably due to proteolysis ... marker in thin sections of human brain In summary, our data indicates that the active c-secretase complex is localized to lipid rafts in human brain Results The c-secretase complex is present in ... detected by binding of cholera toxin subunit B using a dot-blot assay In (C) and (D), the DRMs were isolated after treatment with 1% Triton X-100 of (C) rat brain and (D) SH-SY5Y cells brain onto a Superose...

Ngày tải lên: 30/03/2014, 04:20

... response of the epithelium to injury In asthma the chronic cycle of injury and repair is thought to lead to the deregulation of factors involved, resulting in airway remodeling [57] Our observations ... ng/ml to ng/ml Like the pattern observed in BEAS-2B cells, TGFβ1 induced a fall in E-cadherin and an increase in αSMA mRNA (Figure 6A) in the NHBE cells as well as the corresponding changes in the ... 48 h in contrast to untreated cells (Figure 5A) Next, we assessed the effect of TGFβ1 on cell invasion In an invasion assay utilizing Matrigel™ coated cell inserts we observed up to 100% increased...

Ngày tải lên: 12/08/2014, 14:20

... likely occurring in uninfected/ bystander cells given that a 1.5-fold increase in the number of infected cells is seen at 24 h post-infection with ICAM-1-bearing virions compared to infection with ... lead to massive and indiscriminate induction of apoptosis Instead, it can be postulated that an increase in p53 mRNA might prepare cells to undergo apoptosis rapidly would the presence of an incoming ... leading to induction of apoptosis As discussed above, viral proteins that can bind p53 might inhibit its pro-apoptotic activity, allowing more cells to become either productively or latently infected...

Ngày tải lên: 13/08/2014, 05:21

... assembly at the beginning of the ITGB4BP gene To classify the remaining sites within introns or in intergenic regions, enrichment sites were compared to other gene databases As summarized in Table 1, ... Williams AJ, et al.: Unbiased mapping of transcription factor binding sites along human chromosomes 21 and 22 points to widespread regulation of noncoding RNAs Cell 2004, 116:499-509 Sandoval J, ... bias towards terminal exons over initiating exons (Figure 4) This is probably reflecting the stalling of PolII during the coupled processes of transcription termination and 3'end processing [15]...

Ngày tải lên: 14/08/2014, 14:21

... Fig Increased NHE-1 expression leads to inhibition of staurosporine-induced cell death in NIH3T3 cells 49 Fig Silencing of NHE-1 gene leads to increased susceptibility to cell death in NIH3T3 cells ... correlates with NIH3T3 cells’ sensitivity to staurosporine-induced cell death 51 Fig Silencing of NHE-1 gene leads to increased susceptibility to 54 cell death in U87 cells Fig Time-dependent ... protein expression in U87 cells 63 Fig 12 Inhibition of intracellular superoxide production prevents NHE-1 expression in U87 cells and increases their susceptibility to etoposide-induced cell death...

Ngày tải lên: 16/09/2015, 08:31

... avail- Protein Name Cyclin dependant kinase Cell cycle regulating D-cyclin Cyclin E2 Proliferation of germ cells (POG) Cyclin D-dependant kinase inhibitors p27 Kip1 a Cdk inhibitor Cdk2 CyclinD2 Gcd/Pog ... Cdk inhibitors modulate Cdk activity during the mitotic cell cycle Members of the INK4 family (p15INK4b , p16 INK4a , p18 INK4c , p19INK4d ) specifically bind and regulate cyclin D dependant kinases ... receptor resulted in spermatogenic arrest predominantly at the diplotene stage Also results in low serum testosterone levels leading to azoospermia and infertility Cell Cycle Regulation in Mammalian...

Ngày tải lên: 25/10/2013, 21:20

... (2004) Cell- specific effects of RB or RB/p107 loss on retinal development implicate an intrinsically deathresistant cell- of-origin in retinoblastoma Cancer Cell 5:539–551 Modeling Cell Cycle Control ... Bilateral retinal and brain tumors in transgenic mice expressing simian virus 40 large T antigen under control of the human interphotoreceptor retinoid-binding protein promoter J Cell Biol 119:1681–1687 ... rendering insensitive to p16INK4 inhibition has been reported in melanomas (Zuo et al 1996) Modeling Cell Cycle Control and Cancer with pRB Tumor Suppressor 241 Inhibitors of Cdks or CKIs (cyclin-dependent...

Ngày tải lên: 25/10/2013, 21:20

... signaling, allowing cells to undergo transformation in response to Ras activation Interestingly, human mammary epithelial cells seem to have higher requirements for transformation, being insensitive ... Senescence and Cell Cycle Control 263 induction by controlling p16INK4a (and p19ARF in MEFs), while the detail of the promoter regulation requires further investigations In mouse cells, p19ARF ... checkpoints (Brookes et al 2002, 2004) Therefore, germline disruption of p16INK4a in human cells appears to affect senescence more significantly than in mouse cells Mouse Cells vs Human Cells:...

Ngày tải lên: 25/10/2013, 21:20

... Mitosis inhibitor G1-S transition inhibitor Mitosis inhibitor G1-S cyclin G1-S transition inhibitor G1-S transition inhibitor G1-S transition inhibitor DNA replication DNA replication APC/C inhibitor ... contain an F-box motif near their amino termini and one of several protein-protein interaction motifs carboxy ter- The ubiquitin-proteasome pathway in cell cycle control 157 minal to the F-box In ... Cdc4/Fbw7 does not appear to undergo cell cycle regulation in mammalian cells, it is upregulated in response to genotoxic stress The ubiquitin-proteasome pathway in cell cycle control 163 (Kimura et...

Ngày tải lên: 25/10/2013, 21:20

... during infection, the contribution of the minor proteins to cell destruction via apoptosis is only moderate, suggesting that toxicity of the minor proteins is controlled during the infectious cycle ... the inhibitor was 50 lm Cells were incubated in the presence of the inhibitor, and caspase activity and cell death were measured at the time-points indicated Incubation of cells with actinomycin ... contribute to apoptosis induced during MPyV infection To test whether the minor proteins function as inducers of apoptosis also during infection, we prepared the MPyV genome mutated in ATG codons...

Ngày tải lên: 16/02/2014, 09:20

... [44–46] It is interesting to note that in all cases MEK inhibitor-induced BIM expression alone was not sufficient to induce a dramatic increase in cell death; pronounced increases in apoptosis were ... required, at least in part, for imatinib-induced apoptosis in BCR–ABL-transformed murine progenitor cells [64] and in BCR–ABL+ K562 CML cells in response to both imatinib and nilotinib [65] INNO-406, ... Puma in tyrosine kinase inhibitor-induced apoptosis of human leukemic cells and transduced hematopoietic progenitors carrying oncogenic FLT3 Blood 113, 2302–2311 Kinkade CW, Castillo-Martin M,...

Ngày tải lên: 16/03/2014, 00:20

... adhesion factor in cell cell interaction therefore requires reappraisal [4,23] For instance, platelet binding to dysfunctional endothelium was found to be inhibited by the phosphatidylserine-binding ... life and death In addition to energy supply, they have also been demonstrated to be involved in execution of apoptosis via the release of apoptogenic factors such as cytochrome c [5] Human blood ... Barteggi A & Rosado JA (2006) Thrombin-induced caspases and translocation to the cytoskeleton is independent of changes in cytosolic calcium in human platelets Blood Cells Mol Dis 43, 7978–7985 FEBS...

Ngày tải lên: 16/03/2014, 04:20

... (GlcN)3 binding to the wild-type enzyme increased the transition temperature (Tm) by 5.7 °C, and its binding to D40G increased Tm by 2.8 °C Thus, (GlcN)3 binding to D40G stabilizes the protein structure ... resulting from substrate binding it could be in position to perform catalysis To date, the exact conformational changes occurring after substrate binding could not be described in GH46 chitosanases, ... growth of E coli strains expressing wild-type or V148T chitosanase although inhibiting strains expressing the T45H chitosanase or harboring the empty pAlter-1 vector During the optimization of the...

Ngày tải lên: 16/03/2014, 04:20

... (first rinse once to change the buffer; total volume used is 200 ml) Wash again in MMR and finally in XB Transfer into SW50.1 tubes (Beckman, Palo Alto, CA) containing XB and pack them by spinning ... to histone H1 over amount of kinase Determining Vm~ and Km Dilute the kinase in EB (dilution factor determined in previous step; here we use a kinase solution having an activity of 94 pmol/min/~l ... eukaryotic cell cycle is coordinated by several closely related serine/ threonine protein kinases, each consisting of a catalytic cyclin-dependent kinase (CDK) subunit and a regulatory cyclin subunit.1...

Ngày tải lên: 10/04/2014, 11:13

... recombinant proteins are quite effective at inducing B and Th immunity, viral vectors can be more effective at inducing cytotoxic T cells [14] DNA vaccine vectors offer several advantages, including ... CD62L- Exhausted T cells DNA Priming AntiInfection, Tumor Boosting Immune induction / amplification / re-induction, etc Vector inducing central memory PD-1lo T cells B Vector inducing peripheral ... easily restored through blocking PD-1/PD-1L interaction, speaking to the critical role of PD-1 in determining the fate of CD8+ T cells post-priming (summary of results in refs [42] and [48]) In this...

Ngày tải lên: 18/06/2014, 16:20

... Necrotic cell death (fold change vs control) G cells M cells Apoptotic cell death (fold change vs control) SM cells B 30 20 10 BrP IA 5-FU 15 10 BrP IA FU G- cells ns 0.9 0.6 0.3 * * Control 3BrP ... cell- death pathway in response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G-cells), medium (M-cells) and high (SM-cells) bioenergetic signatures Cells ... Specifically, in M-cells, 3BrP treatment was more effective than IA treatment at triggering cell death (Figure 1) In contrast, cell death in response to 5-FU treatment was found to directly correlate...

Ngày tải lên: 18/06/2014, 19:20

... Necrotic cell death (fold change vs control) G cells M cells Apoptotic cell death (fold change vs control) SM cells B 30 20 10 BrP IA 5-FU 15 10 BrP IA FU G- cells ns 0.9 0.6 0.3 * * Control 3BrP ... cell- death pathway in response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G-cells), medium (M-cells) and high (SM-cells) bioenergetic signatures Cells ... Specifically, in M-cells, 3BrP treatment was more effective than IA treatment at triggering cell death (Figure 1) In contrast, cell death in response to 5-FU treatment was found to directly correlate...

Ngày tải lên: 20/06/2014, 03:20