condensation reaction of ethanol and formic acid

low - level detection of ethanol and h2s with temperature - modulated wo3 nanoparticle gas sensors

low - level detection of ethanol and h2s with temperature - modulated wo3 nanoparticle gas sensors

... transients. After calibration of the sensor for low concentrations of ethanol and H 2 S, it was possible to detect as little as 200 ppb of ethanol and 20 ppb of H 2 S (both of them dry gases) with ... between 150 and 250 ◦ C for all further measure- ments. 3.2. Calibration curves for low concentrations of ethanol and H 2 S At first, different concentrations of ethanol (2, 5, 10, 20, 30, 40, and 50ppm) ... 2004 Abstract Low-level detection of ethanol and H 2 S was achieved with thermally modulated WO 3 nanoparticle gas sensors. Nanoparticle WO 3 films, with a mean grain size of ∼5 nm and a thickness of ∼20 ␮m, were...

Ngày tải lên: 20/03/2014, 13:04

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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"

Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"

... genomics of low concentrations of ethanol in HepG2 cells. (A) Reported interactions of each ethanol- regulated gene studied. (B) Indirect interaction of ITGB4, COBRA1 and ANK3 with ethanol, as ... concentrations of ethanol (100 mM) induced the expression of genes involved in the metabolism of ethanol [16]. In addition, the metabolism of ethanol results in an increased production of toxic ... or the ingestion of ethanol induces a completely different pattern due to the reported ethanol- induced expression and activation of cytokines and chemokines in monocytes and macrophages (including...

Ngày tải lên: 31/10/2012, 15:28

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Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc

Tài liệu Báo cáo khoa học: Exposure of IgG to an acidic environment results in molecular modifications and in enhanced protective activity in sepsis doc

... prevents brisk changes in the levels of components of inflamma- tion, coagulation, and other pathways [21]. The admini- stration of a single dose of 500 mgặkg )1 of the modied IVIg had significant ... Breeding Farm of the Bulgarian Academy of Sciences. The experimental protocols were approved by the Animal Care Commission of the Institute of Microbiology, in accordance with National and European ... CRP and low-pH buffer-exposed IVIg was 69.1 lm. The absence of detectable binding or negligible responses precluded the estimation of reli- able values of kinetic parameters for the native and the...

Ngày tải lên: 16/02/2014, 15:20

12 620 0
Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

Tài liệu Báo cáo khoa học: Purification and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... amida- tion, hydroxylation of Pro, Val or Lys, c-carboxylation of Glu, glycosylation of Ser or Thr, bromination of Trp, sulfation of Tyr, cyclization of N-terminal Gln, and epimerization of several different ... structure of conomarphin was similar to those of other conotoxin cDNAs. The cDNA-encoded precursor of conomarphin consisted of a conserved M-superfamily signal peptide of 25 resi- dues, a proregion of ... side chain of Lys9, Hyp10 and D-Phe13 of cono- marphin is shown in stick mode, and the side chain of L-Phe13 in L-Phe13-conomar- phin is shown as a gray stick. Y. Han et al. A D-amino acid- containing...

Ngày tải lên: 18/02/2014, 17:20

12 617 0
Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

Tài liệu Báo cáo khóa học: Active-site residues and amino acid specificity of the bacterial 4¢-phosphopantothenoylcysteine synthetase CoaB pptx

... Asn210 and Ala276 are important for the second-half reaction and are part of the active-site of CoaB; these residues may be directly involved in binding of one of the substrates, the amino acid cysteine ... 4Â-phosphopantothenic acid, CTP and cysteine. The synthesis of PPC occurs in two half reactions starting with the formation of 4Â-phosphopantothenoyl-CMP (activation of the carboxyl group of 4Â-phosphopantothenate). ... synthesis of 4Â-phosphopantothenoyl-CMP (cysteine, upper part of the gure) and of 4Â-phosphopantothenoylcysteine (+ cysteine, lower part of the figure) by the mutant His-CoaB proteins R206Q and A276V...

Ngày tải lên: 19/02/2014, 12:20

10 491 0
Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

Tài liệu Báo cáo khoa học: The SCO2299 gene from Streptomyces coelicolor A3(2) encodes a bifunctional enzyme consisting of an RNase H domain and an acid phosphatase domain pdf

... PIPES as an internal standard, and 2 lgof protein samples in a final volume of 100 lL. The reaction mixture was incubated for 10 min at 37 °C and transferred to 400 lL cold methanol. The contents ... Biology (Keio University)’ and a grant from New Energy and Industrial Technology Development Organization (NEDO) of the Ministry of Economy, Trade and Industry of Japan (Development of a Technological Infrastructure ... Rv2228c (or MT2287) of Mycobacterium tuberculosis; Mle, ML1637 of Mycobacterium leprae; Mav, MAP1980c of Mycobacterium avium; Nfa, nfa16400 of Nocardia farcinica, and Tfu, Tfus02000308 of Thermobifida...

Ngày tải lên: 19/02/2014, 18:20

10 561 1
Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf

Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf

... architec- ture of OYE1 and PETN reductase, and (ii) geometry for the binding of steroid compounds and 2-cyclohe- xen-1-one [2,19]. The structure of the Y186F enzyme is essentially identical to that of wild-type ... C. Bruce 2 , Andrew W. Munro 1, *, David Leys 1, † and Nigel S. Scrutton 1, † 1 Department of Biochemistry, University of Leicester, UK 2 CNAP, Department of Biology, University of York, UK Pentaerythritol ... 2-cyclohexen-1-one. The H181A and H184A enzymes retain activity in reductive and oxidative half-reactions, and thus do not play an essential role in catalysis. Ligand binding and catalysis is not substantially...

Ngày tải lên: 20/02/2014, 02:21

12 604 0
Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

Tài liệu Báo cáo Y học: The b-1,4-endogalactanase A gene from Aspergillus niger is specifically induced on arabinose and galacturonic acid and plays an important role in the degradation of pectic hairy regions pdf

... producing transformant and was used for the purification of GALA. Growth of this transformant and subsequent purification of the enzyme were performed as described in Materials and methods. Purity of the preparation ... to facilitate purification of GALA. The enzyme has a molecular mass of 48.5 kDa and a pH optimum between 4 and 4.5. Incubations of ara- binogalactans of potato, onion and soy with GALA resulted initially ... Maldi-TOF MS analysis of the hydrolysis of arabinogalactans by GALA. Three different arabinogalactans were used: potato (A), onion (B), and soy (C). Spectra were taken after 15 min (1) and 2 h (2) of...

Ngày tải lên: 21/02/2014, 01:21

9 669 0
Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx

Tài liệu Báo cáo khoa học: A point mutation in the ATP synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction pptx

... absence of Du.(C)Theratioof the best-fitting functions and of the data points of wild-type over mutant are plotted for data in the presence (d)andabsence(m)ofDu. 1988 P. Turina and B. A. Melandri ... synthase of Rhodobacter capsulatus results in differential contributions of DpH and Du in driving the ATP synthesis reaction Paola Turina and B. Andrea Melandri Department of Biology, Laboratory of ... uncoupler- and oligomycin-sensitive and can be attributed to the ATP synthase. This initial rate has been measured as a function of DpH in the presence and absence of Du for both mutant and wild-type...

Ngày tải lên: 21/02/2014, 03:20

9 580 0
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx

Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx

... of nucleotides (Table 1). The rate of CTP synthesis was dramatically influenced by the presence of GTP, and 20 and 41-fold increases in k cat were obtained with GTP concentrations of 0.1 m M and 1m M , ... the basis of the kinetic constants in Table 1, and those obtained for the glutaminase reaction in the presence of 0.1 m M each of UTP and ATP-cS (data not shown). Apparently, the value of K a , ... activation of the glutaminase activity in the presence of 0.1 m M (open circles) or 1 m M (closedcircles)eachofUTPandATP-cS. (C) GTP activation of CTP synthesis at 0.1 m M each of UTP and ATP. 4776...

Ngày tải lên: 21/02/2014, 15:20

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