... other factors and lead to differentKnowledge and Process Management CASE STUDY Transfer of Knowledge and the Role of Motivation 123‘Merchandise’ such as printed T-shirts, armbands and baseball ... between knowledge and suc-cessful knowledge transfer. CONCLUSIONThe purpose of this paper is to increase the under-standing of how knowledge transfers can be per-ceived and managed, and how choices ... 1994. Case study research: Design and methods (2ndedn). Sage: Thousand Oaks, CA.Zander U, Kogut B. 1995. Knowledge and the speed of transfer and imitation of organizational capabilities.An...
... rate and extent of back electron transfer from the ferrous NOSheme to FMNsq following flash photolysis of CO canindicate precisely the rate of electron transfer, but can-not reveal the extent of ... anaccurate measure of the koffparameter in CaM-boundeNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational changerates versus rates of FMNH2formation ... Model and simulations of cytochrome c reduction by NOSenzymes. (A) Scheme of cytochrome c reduction. The model usesfour kinetic rates: dissociation (k1) and association (k2) of the FMNand...
... and pyridine nucleotides is highlydependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of ... Forma-tion of these CTCs appears necessary for efficienthydride transferand the relative conformation and ori-entation of FNR and NADP+⁄ H during the interac-tion are critical [121]. Hydride transfer ... transfer. Mutation of these residues changesFld surface topology, and the module and orientation of the molecular dipole, contributing to their alteredbehaviour. Mutational studies on I59 and...
... betweendonor and acceptor dyes, will therefore unveil the sta-tus of physical union and separation of complementarystrands during renaturation and strand exchange,respectively, and thereby ... labeled Phi-W and rhodamine labeled Phi-C (1) with1.25 lM of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 mM of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ... strand exchange reaction.Effect of homology dependence and ATPhydrolysis on strand exchange activityStrand exchange was monitored as a function of reac-tion time, at a fixed concentration of...
... architec-ture of OYE1 and PETN reductase, and (ii) geometryfor the binding of steroid compounds and 2-cyclohe-xen-1-one [2,19]. The structure of the Y186F enzyme isessentially identical to that of wild-type ... C1 and C2; the double bond between C4 and C5 is not susceptible to reduction, and thusprogesterone and related steroids (e.g. 4-androstene-3,17-dione), which lack a double bond between C1 and ... formation and sub-sequent collapse of an oxidized enzyme–NADPHcharge -transfer complex (Fig. 4A). The rapid forma-tion of the charge -transfer intermediate preventedaccurate analysis of the rate...
... ERresidency of UGT1A mediated by the stop transfer sequence by analysingthe subcellular localization and sensitivity to endoglycosidases of chimericproteins formed by fusion of UGT1A stop transfer ... prevent membrane targeting and insertion of the enzyme. These findings resulted in theidentification of an internal signal sequence localizedbetween residues 140 and 240 and led us to suggestthat ... atpositions )3 and )5 from the C-terminus exposedon the cytosolic side of the ER membrane [18]. Wereport in this study that disruption of the dilysinemotif KSKTH of UGT1A by mutation of lysine...
... of the PSI core complex and the other forintegrity and stabilization of a luminal domain invol-ving at least PSI-N and the N-terminal part of PSI-Fwhich is required for efficient electron transfer. Fig. ... perturbation of theluminal side of PSI involving PSI-F and PSI-N. Theabsence of PSI-J might affect the conformation of PSI-F, which, in turn, changes the binding of PSI-N.PSI-F provides part of the ... between Pc in solution and PSI, and a slow phase corresponding to inactive PSI and acontribution from absorption of oxidized Pc at 834 nm[25–27]. For analysis of wild-type and mutants lackingPSI-J,...
... yielded values for KN of 37 mM and 69 mM and for (Vs/Vh)max of 3 and 10 for thewild-type and bF24A, respectively. This indicates that theimproved kinetics of acyl transferof bF24A are caused ... rate of conversion of the substrate (acyl donor) vs. the rate of conversion of the product (antibiotic), and (b) the rate of acyl transfer to a b-lactam nucleophile vs. the rate of acyl transfer ... mayinfluence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of theenzyme for PAA and derivatives thereof, while maintainingthe hydrophobicity of the binding...
... the deter-mination of both thermodynamic and kinetic properties of NR1, and as we have shown for CPR and P450 BM3(23,24,28) the properties of the flavin binding domains of NR1 are likely to ... 2003 Redox properties of human NR1 (Eur. J. Biochem. 270) 1169Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the humanoxidoreductase ... FMN-bindingdomains of NR1 have been expressed and purified and theirredox properties studied by stopped-flow and steady-statekinetic methods, and by potentiometry. The midpointreduction potentials of the...
... actions: Selection of a pair of words wo E V1 and vo E V2, and a head transducer 3,10 to start the entire derivation. Selection of a pair of dependent words w I and v ~ and transducer M I ... above: ) Selection of a word and acceptor to start an entire derivation. • Selection of a dependent word and acceptor given a head word and a dependency relation. 2.2 Transfer Transfer in this ... Figure 1: Head transducer M converts the sequences of left and right relations (r~ r~) and (r~+l rn 1) of w into left and right relations (r~ r]) and empty symbol e. While it is possible to construct...
... An example of dictionary transfer rules of popular verbs. Grobal sentential structures of Japanese and English are quite different, and correspondingly the internal structure of a Japanese ... sophisticated grammatical and semantic checking, especially when the completeness of semantic analy- sis is not assured and trial -and- error improvements are required at the transferand generation stages. ... Structural transformation of an embedded sentence of type 3. 425 DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION...
... batches of DDGS where approximately 0, 30, 60 and 100% of the maximum possible of syrup was added to the mash before drying. Actual rates of solubles addition to the mash were 0, 12, 25 and 42 ... (lightness of color) and b* (yellowness of color), with an increase in a* (redness of color). Similar results were also reported by Ganesan et al. (2005). Table 1. The Effect of the Rate of Solubles ... Chemical Characteristics of DDGS 2 Particle Size, Bulk Density and pH Particle size and particle size uniformity of feed ingredients are important considerations of livestock and poultry nutritionists...