Ngày tải lên: 05/09/2013, 16:11
... other factors and lead to different Knowledge and Process Management CASE STUDY Transfer of Knowledge and the Role of Motivation 123 ‘Merchandise’ such as printed T-shirts, armbands and baseball ... between knowledge and suc- cessful knowledge transfer. CONCLUSION The purpose of this paper is to increase the under- standing of how knowledge transfers can be per- ceived and managed, and how choices ... 1994. Case study research: Design and methods (2nd edn). Sage: Thousand Oaks, CA. Zander U, Kogut B. 1995. Knowledge and the speed of transfer and imitation of organizational capabilities. An...
Ngày tải lên: 24/01/2014, 00:20
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf
... rate and extent of back electron transfer from the ferrous NOS heme to FMNsq following flash photolysis of CO can indicate precisely the rate of electron transfer, but can- not reveal the extent of ... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational change rates versus rates of FMNH 2 formation ... Model and simulations of cytochrome c reduction by NOS enzymes. (A) Scheme of cytochrome c reduction. The model uses four kinetic rates: dissociation (k 1 ) and association (k 2 ) of the FMN and...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc
... and pyridine nucleotides is highly dependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of ... Forma- tion of these CTCs appears necessary for efficient hydride transfer and the relative conformation and ori- entation of FNR and NADP + ⁄ H during the interac- tion are critical [121]. Hydride transfer ... transfer. Mutation of these residues changes Fld surface topology, and the module and orientation of the molecular dipole, contributing to their altered behaviour. Mutational studies on I59 and...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: DNA strand exchange activity of rice recombinase OsDmc1 monitored by fluorescence resonance energy transfer and the role of ATP hydrolysis pptx
... between donor and acceptor dyes, will therefore unveil the sta- tus of physical union and separation of complementary strands during renaturation and strand exchange, respectively, and thereby ... labeled Phi-W and rhodamine labeled Phi-C (1) with 1.25 l M of OsDmc1 in absence of ATP; (2) with 1.25 lM of OsDmc1 in presence of 2.0 m M of AMP-PNP; (3) with 1.25 lM of OsDmc1 in presence of 2.0 mM ... strand exchange reaction. Effect of homology dependence and ATP hydrolysis on strand exchange activity Strand exchange was monitored as a function of reac- tion time, at a fixed concentration of...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khoa học: Proton transfer in the oxidative half-reaction of pentaerythritol tetranitrate reductase Structure of the reduced enzyme-progesterone complex and the roles of residues Tyr186, His181 and His184 pdf
... architec- ture of OYE1 and PETN reductase, and (ii) geometry for the binding of steroid compounds and 2-cyclohe- xen-1-one [2,19]. The structure of the Y186F enzyme is essentially identical to that of wild-type ... C1 and C2; the double bond between C4 and C5 is not susceptible to reduction, and thus progesterone and related steroids (e.g. 4-androstene- 3,17-dione), which lack a double bond between C1 and ... formation and sub- sequent collapse of an oxidized enzyme–NADPH charge -transfer complex (Fig. 4A). The rapid forma- tion of the charge -transfer intermediate prevented accurate analysis of the rate...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx
... ER residency of UGT1A mediated by the stop transfer sequence by analysing the subcellular localization and sensitivity to endoglycosidases of chimeric proteins formed by fusion of UGT1A stop transfer ... prevent membrane targeting and insertion of the enzyme. These findings resulted in the identification of an internal signal sequence localized between residues 140 and 240 and led us to suggest that ... at positions )3 and )5 from the C-terminus exposed on the cytosolic side of the ER membrane [18]. We report in this study that disruption of the dilysine motif KSKTH of UGT1A by mutation of lysine...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... of the PSI core complex and the other for integrity and stabilization of a luminal domain invol- ving at least PSI-N and the N-terminal part of PSI-F which is required for efficient electron transfer. Fig. ... perturbation of the luminal side of PSI involving PSI-F and PSI-N. The absence of PSI-J might affect the conformation of PSI-F, which, in turn, changes the binding of PSI-N. PSI-F provides part of the ... between Pc in solution and PSI, and a slow phase corresponding to inactive PSI and a contribution from absorption of oxidized Pc at 834 nm [25–27]. For analysis of wild-type and mutants lacking PSI-J,...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf
... yielded values for K N of 37 m M and 69 m M and for (V s /V h ) max of 3 and 10 for the wild-type and bF24A, respectively. This indicates that the improved kinetics of acyl transfer of bF24A are caused ... rate of conversion of the substrate (acyl donor) vs. the rate of conversion of the product (antibiotic), and (b) the rate of acyl transfer to a b-lactam nucleophile vs. the rate of acyl transfer ... may influence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the hydrophobicity of the binding...
Ngày tải lên: 17/03/2014, 23:20
Báo cáo khoa học: Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase NR1 doc
... the deter- mination of both thermodynamic and kinetic properties of NR1, and as we have shown for CPR and P450 BM3 (23,24,28) the properties of the flavin binding domains of NR1 are likely to ... 2003 Redox properties of human NR1 (Eur. J. Biochem. 270) 1169 Determination of the redox potentials and electron transfer properties of the FAD- and FMN-binding domains of the human oxidoreductase ... FMN-binding domains of NR1 have been expressed and purified and their redox properties studied by stopped-flow and steady-state kinetic methods, and by potentiometry. The midpoint reduction potentials of the...
Ngày tải lên: 23/03/2014, 21:20
Báo cáo khoa học: "A Comparison of Head Transducers and Transfer for a Limited Domain Translation Application" pptx
... actions: Selection of a pair of words wo E V1 and vo E V2, and a head transducer 3,10 to start the entire derivation. Selection of a pair of dependent words w I and v ~ and transducer M I ... above: ) Selection of a word and acceptor to start an entire derivation. • Selection of a dependent word and acceptor given a head word and a dependency relation. 2.2 Transfer Transfer in this ... Figure 1: Head transducer M converts the sequences of left and right relations (r~ r~) and (r~+l rn 1) of w into left and right relations (r~ r]) and empty symbol e. While it is possible to construct...
Ngày tải lên: 24/03/2014, 03:21
Báo cáo khoa học: "DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION PROJECT" potx
... An example of dictionary transfer rules of popular verbs. Grobal sentential structures of Japanese and English are quite different, and correspondingly the internal structure of a Japanese ... sophisticated grammatical and semantic checking, especially when the completeness of semantic analy- sis is not assured and trial -and- error improvements are required at the transfer and generation stages. ... Structural transformation of an embedded sentence of type 3. 425 DEALING WITH INCOMPLETENESS OF LINGUISTIC KNOWLEDGE IN LANGUAGE TRANSLATION TRANSFER AND GENERATION STAGE OF MU MACHINE TRANSLATION...
Ngày tải lên: 31/03/2014, 17:20
convective heat transfer mathematical and computational modelling of viscous fluids and porous media
Ngày tải lên: 01/04/2014, 10:51
principles and modern applications of mass transfer operations, second edition
Ngày tải lên: 02/04/2014, 15:44
mass and heat transfer analysis of mass contactors and heat exchangers (cambridge series in chemical engineering)
Ngày tải lên: 02/04/2014, 15:52
Báo cáo hóa học: " TRANSFER POSITIVE HEMICONTINUITY AND ZEROS, COINCIDENCES, AND FIXED POINTS OF MAPS IN TOPOLOGICAL VECTOR SPACES" pdf
Ngày tải lên: 23/06/2014, 00:20
Báo cáo hóa học: " Warped Linear Prediction of Physical Model Excitations with Applications in Audio Compression and Instrument Synthesis" pdf
Ngày tải lên: 23/06/2014, 01:20
founding and evaluating efficiency of training model to transfer technology from polyclinic hospital of hoa binh province to district hospitals
Ngày tải lên: 25/07/2014, 13:57